精緻農業品管用拋棄式生物感測試片之開發

Abstract

本研究開發兩種拋棄式檢測試片應用於茶品與蝴蝶蘭的品質管理。茶品品管檢測試片係利用拋棄式網印碳電極搭配流動注射系統檢測茶湯的氧化還原電位，藉此評定茶品發酵度。此法可減少電極表面上多元酚類的吸附(CV<1.2%)且達到高通量檢測 (樣品檢測數量 >20 hr-1)。透過檢測不同發酵度之自製包種茶，顯示此法與高效能液相層析法呈現高度相關性。 本研究另一檢測試片為蘭花蕙蘭嵌紋病毒(CymMV)檢測用免疫層析試片。將多株抗體結合40nm金粒子，可利用肉眼觀察到抗原抗體結合之呈色變化。本研究中，利用肌紅蛋白固定於金奈米粒子，透過肌紅蛋白內之heme基來增強luminol- H2O2 之化學發光強度，藉此降低試片檢測極限。

Two types of disposable sensing strips were developed for the quality control of two economic agricultural products, tea and moth orchids. The sensing strip for tea quality is a screen-printed carbon-paste disposable electrode that can be incorporated into a flow injection manifold to estimate tea fermentation degrees based on the redox potentials of tea infusions. The flow-injection analytical manner reduced the adsorption of polyphenols on the electrode surface, reproducible (CV<1.2%) and high-throughput analytical results (>20 hr-1) were obtained. For pouchong tea samples with different fermentation degrees, the data show a high correlation with those obtained by HPLC. The second one is an immunochromatographic strip for detecting the Cymbidium mosaic virus in moth orchids (phalaenopsis spp.). To visualize the immunochromatographic process, polyclonal antibodies against the coat protein of the virus were attached onto 40nm gold nanoparticles. In the present study, myoglobin was immobilized onto the gold nanoparticles to enhance the luminol-H2O2 chemiluminescence by the prosthetic heme group of myoglobin.