請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25836完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王淑美 | |
| dc.contributor.author | Yi-Shan Wu | en |
| dc.contributor.author | 吳怡珊 | zh_TW |
| dc.date.accessioned | 2021-06-08T06:32:40Z | - |
| dc.date.copyright | 2006-07-28 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-21 | |
| dc.identifier.citation | 1. 林傑, 柯立祥, 洪震國 (1999) 文心蘭栽培管理及採後處理。屏東科技大學農推會農業推展手冊21號。
2. 林立傑 (2004) 高溫影響文心蘭唇瓣發育之相關基因的選殖。國立台灣大學植物科學研究所碩士論文。 3. 陳加忠, 許玉妹 (2003) 累積溫度對文心蘭開花品質之影響。農林學報 52(1)：33-48。 4. Ali MB, Hahn EJ, Paek KY (2005) Effects of temperature on oxidative stress defense systems, lipid peroxidation and lipoxygenase activity in Phalaenopsis. Plant Physiol Biochem 43: 213-223 5. Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53: 1331-1341 6. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276-287 7. Bowler C, Montagu MV, Inz D (1992) Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol plant Mol Biol 43: 83-116 8. Campbell JL, Klueva NY, Zheng H, Nieto-Sotelo J, Ho THD, Nguyen HT (2001) Cloning of new members of heat shock protein HSP101 gene family in wheat (Triticum aestivum (L.) Moench) inducible by heat, dehydration, and ABA. Biochim Biophys Acta 1517: 270–277 9. Cao Y, Song F, Goodman RM, Zheng Z (2006) Molecular characterization of four rice genes encoding ethylene-responsive transcriptional factors and their expressions in response to biotic and abiotic stress. J Plant Physiol (in press) 10. Chase MW（1992）The Oncidium alliance of the Caribbean. Amer Orchid Soc Bull 99-103 11. Choi SM, Jeong SW, Jeong WJ, Kwon SY, Chow WS, Park YI (2002) Chloroplast Cu/Zn-superoxide dismutase is a highly sensitive site in cucumber leaves chilled in the light. Planta 216: 315-324 12. Clarke SM, Mur LA J, Wood JE, Scott, IM (2004) Salicylic acid dependent signaling promotes basal thermotolerance but is not essential for acquired thermotolerance in Arabidopsis thaliana. Plant J 38: 432-447 13. Crafts-Brandner SJ, Law RD (2000) Effect of heat stress on the inhibition and recovery of the ribulose-1,5-bisphosphate carboxylase/oxygenase activation state. Planta 212: 67-74 14. Dat JF, Foyer CH, Scott IM (1998) Changes in salicylic acid and antioxidants during induction of thermotolerance in mustard seedlings. Plant Physiol 118: 1455-1461 15. Dominguez-Solis JR, Gutierrez-Alcala G, Vega JM, Romero LC, Gotor C (2001) The cytosolic O-acetylserine(thiol)lyase gene is regulated by heavy metals and can function in cadmium tolerance. J Biol Chem 276: 9297-9302 16. Droog F (1997) Plant glutathione S-transferases, a tale of theta and tau. J Plant Growth Regul 16: 95-107 17. Elstner, EF (1991) “Mechanisms of oxygen activation in different compartments of plant cells: In Active Oxygen/Oxidative Stress and Plant Metabolism. Pell EJ and Steffen KL (eds) American Soc Plant Physiol Rockville, M.D. pp. 13-25. 18. Federico R, Medda R, Floris G (1985) Superoxide dismutase from Lens esculenta: purification and properties. Plant Physiol 78: 357-358 19. Feller U, Crafts-Brandner SJ, Salvucci ME (1998) Moderately high temperatures inhibit ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) activase-mediated activation of Rubisco. Plant Physiol 116: 539-546 20. Fridovich I (1986) Superoxide dismutases. Adv Enzymol Relat Areas Mol Biol 58: 61–97 21. Fujibe T, Saji H, Arakawa K, Yabe N, Takeuchi Y, Yamamoto KT (2004) A methyl viologen-resistant mutant of Arabidopsis, which is allelic to ozone-sensitive rcd1, is tolerant to supplemental ultraviolet-B irradiation. Plant Physiol 134: 275–285 22. Gomez JM, Jimenez A, Olmos E, Sevilla F (2004) Location and effects of long-term NaCl stress on superoxide dismutase and ascorbate peroxidase isoenzymes of pea (Pisum sativum cv Puget) chloroplasts. J Exp Bot 55: 119-130 23. Gounaris K, Brain AR, Quinn PJ, Williams WP (1984) Structural reorganisation of chloroplast thylakoid membranes in response to heat stress. Biochim Biophys Acta 766: 198–208 24. Gupta AS, Heinen JL, Holaday AS, Burke JJ, Allen RD (1993) Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. Proc Natl Acad Sci USA 90: 1629-1633 25. Havaux M, Greppin H, Strasser RJ (1991) Functioning of photosystem I and photosystem II in pea leaves exposed to heat stress in the presence or absence of light. Planta 186: 88–98 26. Hew CS, Yong JWH（1994）Growth and photosynthesis of Oncidium “Goldiana”. J Hort Sci 69: 809-819 27. Hong SW, Vierling E (2000) Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress. Proc Natl Acad Sci USA 97: 4392–4397 28. Hong SW, Vierling E (2001) Hsp101 is necessary for heat tolerance but dispensable for development and germination in the absence of stress. Plant J 27: 25 35 29. Houter NC, Pons TL (2005) Gap size effects on photoinhibition in understorey saplings in tropical rainforest. Plant Ecology 179: 43-51 30. Jurivich DA, Sistonen L, Kroes RA, Morimoto RI (1992) Effect of sodium salicylate on the human HS response. Science 255: 1243-1245 31. Kaiser R（1993） Orchids of the American tropics. In：The Scent of Orchid Roche Bacel ed 115-116 32. Kaminaka H, Morita S, Tokumoto M, Masumura T, Tanaka K (1999) Differential gene expression of rice superoxide dismutase isoforms to oxidative and environmental stresses. Free Radic Res 31: 219–225 33. Kanematsu S, Asada K (1989) Cu/Zn SOD in rice: occurrence of an active monomeric enzyme and two types of isozyme in leaf and non photosynthetic tissue. Plant Cell Physiol 30: 381–391 34. Kardish N, Magal N, Aviv D, Galun E (1994) The tomato gene for the chloroplastic Cu/Zn superoxide dismutase: regulation of expression imposed in transgenic tobacco plants by a short promoter. Plant Mol Biol 25: 887-897 35. Kliebenstein DJ, Monde RA, Last RL (1998) Superoxide Dismutase in Arabidopsis: An eclectic enzyme family with disparate regulation and protein localization. Plant Physiol 118: 637-650 36. Larkindale J, Knight MR (2002) Protection against heat stress-induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol 128: 682–695 37. Levitt J (1980) Responses of Plants to Environmental Stress, Vol. 1: Chilling, Freezing and High Temperature Stress. Academic Press, New York, NY, pp. 137–141 38. Lu CM, Zhang JH (2000) Heat-induced multiple effects on PSII in wheat plants. J Plant Physiol 156: 259–265 39. May MJ, Vernoux T, Leaver C, Van MM, Inzé D (1998) Glutathione homeostasis in plants: implications for environmental sensing and plant development. J Exp Bot 49: 649-667 40. Mittler R, Zilinskas BA (1994) Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought and following recovery from drought. Plant J 5: 397-405 41. Mittler R, Vanderauwera S, Gollery M, Van-Breusegem F (2004) The reactive oxygen gene network of plants. Trends Plant Sci 9: 490-498 42. Nieto-Sotelo J, Martínez LM, Ponce G, Cassab GI, Alagón A, Meeley RB, Ribaut JM, Yanga R (2002) Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633 43. Ogawa K, Kenematsu K, Takabe K, Asada K (1995) Attachment of CuZn-superoxide dismutase to thylakoid membranes at the superoxide generation (PSI) in spinach chloroplasts: detection by immuno-gold labeling after rapid freezing and substitution method. Plant Cell Physiol 36: 565-573 44. Ogawa K, Kanematsu S, Asada K (1996) Intra- and extra-celluar localization of ‘cytosolic’ CuZn superoxide dismutase in spinach leaf and hypocotyls: their association with lignification. Plant Cell Physiol 37: 790-799 45. Pastori GM, Foyer CH (2002) Common components, networks and pathways of cross-tolerance to stress the central role of 'redox' and abscisic acid-mediated controls. Plant Physiol 129: 460-468 46. Perl-Treves R, Galun E (1991) The tomato Cu,Zn superoxide dismutase genes are developmentally regulated and respond to light and stress. Plant Mol Biol 17: 745-760 47. Perl A, Perl-Treves R, Galili S, Aviv D, Shalgi E, Malkin S, Galun E (1993) Enhanced oxidative-stress defence in transgenicthat overexpress chloroplastic Cu/Zn superoxide dismutase. Proc Natl Acad Sci USA 90: 1629-1633 48. Rizhsky L, Liang H, Mittler R (2003) The water-water cycle is essential for chloroplast protection in the absence of stress. J Biol Chem 278: 38921–38925 49. Schinkel H, Streller S, Wingsle G (1998) Multiple forms of extracellular superoxide dismutase in needles, stem tissues and seedlings of Scots pine. J Exp Bot 49: 931-936 50. Stevens R, Creissen G, Mullineaux PM (1997) Cloning and characterization of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L) and its expression in response to stress. Plant Mol Biol 35: 641-654 51. Swidzinski JA, Sweetlove LJ, Leaver CJ (2002) A custom microarray analysis of gene expression during programmed cell death in Arabidopsis thaliana. Plant J 30: 431-446 52. Vacca RA, De-Pinto MC, Valenti D, Passarella S, Marra E, De-Gara L (2004) Production of reactive oxygen species, alteration of cytosolic ascorbate peroxidase and impairment of mitochondria metabolism are early events in heat shock-induced programmed cell death in tobacco bright-Yellow 2 cells. Plant Physiol 134: 1100-1112 53. Vallelian-Bindschedler L, Schweizer P, Mosinger E, Metraux JP (1998) Heat induced resistance in barley to powdery mildew (Blumeria graminis f sp hordei) is associated with a bust of AOS. Physiol Mol Plant Pathol 52: 185-199 54. Yong JWH, Hew CS （1995） Partitioning of 14C assimilates between sources and sinks during different growth stages in the sympodial thin-leaved orchid Oncidium goldiana Int. J Plant Sci 156: 188-196 55. Youssefian S, Nakamura M, Orudgev E, Kondo N (2001) Increased cysteine biosynthesis capacity of transgenic tobacco overexpressing an O-acetylserine(thiol) lyase modifies plant responses to oxidative stress. Plant Physiol 126: 1001-1011 56. Walker JL, McLennan KM, Robinson DS (1991) Isolation and purification of superoxide dismutase purified from brussels sprouts (Brassica oleracea L var bullata sub var gemmifera). Food Chem 41: 1–9 57. Wang H, Schotz MC (2002) The lipase gene family. J Lipid Res 43: 993-999 58. Winkel BSJ (2004) Metabolic channeling in plants. Annu Rev Plant Biol 55: 85–107 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25836 | - |
| dc.description.abstract | 文心蘭在夏季高溫的環境下，可縮短花芽發育期，但花序之品質卻明顯降低，花軸分叉數和總花朵數都顯著減少，部分花朵亦有畸形的現象，且花軸長度也明顯短於在常溫下生長的花序。迄今我們對於影響文心蘭花序品質的機制毫無所知。為期改善夏季高溫影響花序發育的情形，本論文選殖在高溫下影響文心蘭花軸品質之相關基因，做為日後研究之基礎。實驗採用文心蘭（Oncidium）切花品種南西（Gower Ramsey）三年株齡之植物個體為材，分別培養在適溫（28/23℃）及高溫（35/30℃）之下，然後取其花軸尖端部位，利用DDRT-PCR（differential display reverse transcription PCR）方法，選殖獲得298個基因建構體，經定序比對，再從中挑選與細胞氧化逆境相關之基因具有高度相似性者，期望以轉殖株測試轉殖之基因在高溫下的生理功能。針對數個與氧化逆境相關的基因（BEAM09、BFAM09、BHGM05、BHGM22、BIAM14）經由5’RACE (rapid amplification of cDNA ends)的技術得到文心蘭3個基因lipase (class3) (Olipase)、cysteine synthase (OOASTL)及chloroplast Cu-Zn superoxide dismutase(OSOD)之cDNA序列，進一步將HSP101:: OSOD及HSP101:: antiOSOD構築於pBI101中轉殖至阿拉伯芥，連同阿拉伯芥葉綠體型CuZnSOD(csd2)突變株一同觀察高溫逆境下的基因表現和外表型態。另外，依不同型態的SOD對於抑制劑的敏感性和電泳中移動速率之快慢來鑑定SOD同功酶，可知文心蘭至少有四個CuZnSOD、一個FeSOD和一個MnSOD，這六個SOD同功酶在文心蘭不同組織部位的表現強弱也不盡相同。即使文心蘭和阿拉伯芥的葉綠體型CuZnSOD都會受到高溫逆境的誘導，但是高溫仍會對文心蘭和阿拉伯芥造成光氧化逆境傷害。CSD2突變株意外地有較野生型高的CSD2表現，然而文心蘭SOD或CSD2在高溫環境下阿拉伯芥中的表現強弱雖然對於植株外表型態沒有直接的影響，但是可有助於抵抗部分的光氧化逆境，並延緩高溫對阿拉伯芥所造成的傷害。對於阿拉伯芥或文心蘭在高溫所表現的花序差異目前沒有詳細的研究報導，本文初步探討受高溫影響之文心蘭花序品質的相關基因及文心蘭SOD在高溫逆境可能參與的角色，影響文心蘭花序品質的詳細機制尚待進一步的研究。 | zh_TW |
| dc.description.abstract | Oncidium Gower Ramsey is one of major cut flower species in Taiwan, and the flower production is all over the year. However, high temperature of summer and autumn results in the poor quality of cut flowers, by that not only the branching and floral number of inflorescence drop severely, but the length of inflorescence is shorter than that developed in cool seasons. In order to understand high temperature-related genes in inflorescence of Oncidium, DDRT-PCR technique was used. 298 gene fragments shown differential expression were cloned and sequenced. After Blast alignment, six clones possessing high homology with known genes related to oxidative stress were selected. Among several oxidative stress related genes, three complete cDNA sequences coding for Oncidium lipase(Olipase)、cysteine synthase(OOASTL) and chloroplast CuZnSOD(OSOD) were cloned by 5’RACE. For high temperature stress, AtHSP101 promoter were used to drive sense and antisense OSOD in Arabidopsis thaliana. Even though OSOD and CSD2 (chloroplast CuZnSOD of Arabidopsis) were induced by high temperature, photooxidative stress still occurred in Oncidium and Arabidopsis. csd2 mutant unexpectedly had higher expression of CSD2 than wild type, and had more branch number in both moderate and high growth temperature(35℃). The phenotype of Arabidopsis is not directly affected by OSOD or CSD2, but the enhancement of the active oxygen-scavenging system that leads to increased oxidative stress protection and delayed the harm caused by high temperature in transgenic plants. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T06:32:40Z (GMT). No. of bitstreams: 1 ntu-95-R92b42018-1.pdf: 1525168 bytes, checksum: 44dc6d8def03855532e4dfef9622fce0 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 目 錄
中文摘要…………………………………………………………………..1 英文摘要…………………………………………………………………..2 前言………………………………………………………………………..3 材料與方法…………………………………………………......................9 結果…………………………………………………………....................25 一、 文心蘭DDRT差異性片段選殖定序與序列比對…………………….25 二、 與氧化逆境相關之基因的全長選殖………………………………...26 三、 文心蘭高溫下生理的差異性表現…………………………………...27 四、 阿拉伯芥轉殖株的分析……………………………………………..28 五、 阿拉伯芥轉殖株於高溫下之表現…………………………………...29 討論…………………………………………………………....................31 參考文獻……………………………………………………....................36 圖表…………………………………………………………....................41 附圖…………………………………………………………....................64 | |
| dc.language.iso | zh-TW | |
| dc.subject | 超氧歧化酶 | zh_TW |
| dc.subject | 氧化逆境 | zh_TW |
| dc.subject | 活性氧 | zh_TW |
| dc.subject | ROS | en |
| dc.subject | heat stress | en |
| dc.subject | SOD | en |
| dc.title | 受高溫影響之文心蘭葉綠體型銅鋅超氧歧化酶基因表現之分析 | zh_TW |
| dc.title | Functional Analysis of a Chloroplast Copper/Zinc Superoxide Dismutase of Oncidium Gower Ramsey under Heat Stress | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳枝乾,林讚標 | |
| dc.subject.keyword | 氧化逆境,活性氧,超氧歧化酶, | zh_TW |
| dc.subject.keyword | ROS,SOD,heat stress, | en |
| dc.relation.page | 65 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2006-07-24 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-95-1.pdf 未授權公開取用 | 1.49 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。