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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡懷楨(Huai-Jen Tsai) | |
| dc.contributor.author | En-Sheng Dai | en |
| dc.contributor.author | 戴恩聖 | zh_TW |
| dc.date.accessioned | 2021-06-08T06:26:10Z | - |
| dc.date.copyright | 2006-07-31 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2006-07-27 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25713 | - |
| dc.description.abstract | alpha-2 macroglobulin在對蝦為一血淋巴球專一性且具免疫可誘導性表現的基因，本實驗中由草蝦血球萃取genomic DNA，並建立一嵌入片段長度範圍約11.5 kb至14.5 kb，平均長度約13 kb的genomic DNA library (1×108 pfu/ml)。利用extender PCR及library screening的方式分別獲得在轉譯起始位前535 bp和1.2 kb的alpha-2 macroglobulin DNA片段；將此兩DNA片段構築在EGFP綠螢光報導基因後進行斑馬魚卵顯微注射，結果顯示兩DNA片段皆可驅動EGFP綠螢光報導基因在斑馬魚表現，表現率分別為30%和40%(n=300)，在螢光表現率上1.2 kb片段表現能力較535 bp片段高出約10%；而在表現綠螢光的個體中，兩不同長度的片段均有70%為專一性強表現在肌肉束上。利用果蠅類巨噬細胞Schneider 2 (S2)細胞株進行轉染實驗，此兩DNA片段皆可驅動EGFP及firefly luciferase報導基因的表現；而在luciferase活性測試上，若以未含有啟動子的pGL3-basic empty vector為基礎比較，535 bp片段和1.2 kb片段表現能力分別為110倍(SD=16.3)和150倍(SD=18.0)。由上述斑馬魚螢光表現率和S2細胞luciferase活性測試，可推測1.2 kb的片段中可能包含有535 bp片段所沒有的cis-element來加強基因的表現。藉由斑馬魚系統和果蠅S2細胞株的實驗，可以證明草蝦A2M基因轉譯起始位前1.2 kb的DNA片段為一具有驅動基因強表現的DNA片段。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-08T06:26:10Z (GMT). No. of bitstreams: 1 ntu-94-R93b43014-1.pdf: 3308775 bytes, checksum: 2ff674a2e62305d442ef265dba121cd6 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………………… 1
英文摘要……………………………………………………………… 2 引言…………………………………………………………………… 3 實驗材料……………………………………………………………… 9 實驗方法……………………………………………………………… 14 結果…………………………………………………………………… 29 討論…………………………………………………………………… 35 參考資料……………………………………………………………… 41 圖表…………………………………………………………………… 52 | |
| dc.language.iso | zh-TW | |
| dc.subject | 對蝦 | zh_TW |
| dc.subject | 斑馬魚 | zh_TW |
| dc.subject | (S2)細胞株 | zh_TW |
| dc.subject | A2M基因 | zh_TW |
| dc.subject | genomic DNA library | en |
| dc.subject | luciferase | en |
| dc.subject | alpha-2 macroglobulin | en |
| dc.title | 草蝦alpha-2 macroglobulin基因啟動子的選殖與分析 | zh_TW |
| dc.title | Cloning and Analysis of the Alpha-2 Macroglobulin Promoter
of the Black Tiger Shrimp (Penaeus monodon) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡惠珍(Huey-Jen Tsay),繆希椿(Shi-Chuen Miaw),陳曜鴻(Yau-Hung Chen) | |
| dc.subject.keyword | A2M基因,對蝦,(S2)細胞株,斑馬魚, | zh_TW |
| dc.subject.keyword | alpha-2 macroglobulin,genomic DNA library,luciferase, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2006-07-28 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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