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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃義侑 | |
| dc.contributor.author | Chin Hui Li | en |
| dc.contributor.author | 李靚蕙 | zh_TW |
| dc.date.accessioned | 2021-06-08T06:22:17Z | - |
| dc.date.copyright | 2006-07-31 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-31 | |
| dc.identifier.citation | Reference
chang, c.-l. (2005). lysophosphatidic acid induces interleukin 1β expression, reactive oxygen species production, and oxidized LDL uptake in mouse macrophages. institute of zoology. taipei. Das, A. K., S. Horie, et al. (1992). 'Biosynthesis of glycerolipid precursors in rat liver peroxisomes and their transport and conversion to phosphatidate in the endoplasmic reticulum.' J Biol Chem 267(14): 9724-30. Gaits, F., O. Fourcade, et al. (1997). 'Lysophosphatidic acid as a phospholipid mediator: pathways of synthesis.' FEBS Lett 410(1): 54-8. Gizeli, E. and C. R. Lowe (2002). Biomolecular sensors. London ; New York, Taylor & Francis. Goetzl, E. J. and S. An (1998). 'Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate.' Faseb J 12(15): 1589-98. Gschwind, A., N. Prenzel, et al. (2002). 'Lysophosphatidic acid-induced squamous cell carcinoma cell proliferation and motility involves epidermal growth factor receptor signal transactivation.' Cancer Res 62(21): 6329-36. Inoue, C. N., M. Epstein, et al. (1999). 'Lysophosphatidic acid and mesangial cells: implications for renal diseases.' Clin Sci (Lond) 96(4): 431-6. Jalink, K., E. J. van Corven, et al. (1990). 'Lysophosphatidic acid, but not phosphatidic acid, is a potent Ca2(+)-mobilizing stimulus for fibroblasts. Evidence for an extracellular site of action.' J Biol Chem 265(21): 12232-9. Koh, J. S., W. Lieberthal, et al. (1998). 'Lysophosphatidic acid is a major serum noncytokine survival factor for murine macrophages which acts via the phosphatidylinositol 3-kinase signaling pathway.' J Clin Invest 102(4): 716-27. Kue, P. F., J. S. Taub, et al. (2002). 'Lysophosphatidic acid-regulated mitogenic ERK signaling in androgen-insensitive prostate cancer PC-3 cells.' Int J Cancer 102(6): 572-9. Lee, H., E. J. Goetzl, et al. (2000). 'Lysophosphatidic acid and sphingosine 1-phosphate stimulate endothelial cell wound healing.' Am J Physiol Cell Physiol 278(3): C612-8. Lin, J. H. (2005). study on the fabrication and application of a surface acoustic wave biosensor. graduate institute of microbiology and biochemistry. taipei, national taiwan university. Liu, Y. C., C. M. Wang, et al. (2001). 'Comparison of different protein immobilization methods on quartz crystal microbalance surface in flow injection immunoassay.' Anal Biochem 299(2): 130-5. Mills, G. B. and W. H. Moolenaar (2003). 'The emerging role of lysophosphatidic acid in cancer.' Nat Rev Cancer 3(8): 582-91. Minami, N., A. Kawasaki, et al. (2003). PROC. 51st Annual Conference on Mass Spectrometry: 14-16. Moolenaar, W. H., L. A. van Meeteren, et al. (2004). 'The ins and outs of lysophosphatidic acid signaling.' Bioessays 26(8): 870-81. Neves, S. R., P. T. Ram, et al. (2002). 'G protein pathways.' Science 296(5573): 1636-9. Noguchi, Y., A. Okamoto, et al. (2006). 'Lysophosphatidic acid cooperates with EGF in inducing branching morphogenesis of embryonic mouse salivary epithelium.' Dev Dyn 235(2): 403-10. Pages, C., M. F. Simon, et al. (2001). 'Lysophosphatidic acid synthesis and release.' Prostaglandins Other Lipid Mediat 64(1-4): 1-10. Sasagawa, T., K. Suzuki, et al. (1998). 'The significance of plasma lysophospholipids in patients with renal failure on hemodialysis.' J Nutr Sci Vitaminol (Tokyo) 44(6): 809-18. Shida, D., J. Kitayama, et al. (2005). 'Lysophospholipids transactivate HER2/neu (erbB-2) in human gastric cancer cells.' Biochem Biophys Res Commun 327(3): 907-14. Si, S., L. Si, et al. (2002). 'Study of adsorption behavior of bilirubin on human-albumin monolayer using a quartz crystal microbalance.' J Colloid Interface Sci 253(1): 47-52. Storri, S., T. Santoni, et al. (1998). 'surface modifications for the development of piezoimmunosensors.' Biosensors & Bioelectronics 13: 347-357. Takeda, H., A. Kawasaki, et al. (2003). 'Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of phosphorylated compounds using a novel phosphate capture molecule.' Rapid Commun Mass Spectrom 17(18): 2075-81. Tanaka, T., H. Tsutsui, et al. (2004). 'Quantitative analysis of lysophosphatidic acid by time-of-flight mass spectrometry using a phosphate-capture molecule.' J Lipid Res 45(11): 2145-50. Thumser, A. E., J. E. Voysey, et al. (1994). 'The binding of lysophospholipids to rat liver fatty acid-binding protein and albumin.' Biochem J 301 ( Pt 3): 801-6. Tigyi, G. and R. Miledi (1992). 'Lysophosphatidates bound to serum albumin activate membrane currents in Xenopus oocytes and neurite retraction in PC12 pheochromocytoma cells.' J Biol Chem 267(30): 21360-7. Vallee, B. (1986). In Zinc Enzymes. Boston, Birkhauser. van Meeteren, L. A., F. Frederiks, et al. (2004). 'Spider and bacterial sphingomyelinases D target cellular lysophosphatidic acid receptors by hydrolyzing lysophosphatidylcholine.' J Biol Chem 279(12): 10833-6. Velasco-Garcia, M. N. and T. Mottram (2003). 'biosensor technology addressing agriculture problems.' biosystems engineering 84: 1-12. Vogt, W. (1963). 'Pharamacologically active acidic phospholipids and glycolipids.' Biochem Pharmacol 12: 415-20. Weiner, J. A. and J. Chun (1999). 'Schwann cell survival mediated by the signaling phospholipid lysophosphatidic acid.' Proc Natl Acad Sci U S A 96(9): 5233-8. Wilkinson, T. C. and D. C. Wilton (1986). 'Studies on fatty acid-binding proteins. The detection and quantification of the protein from rat liver by using a fluorescent fatty acid analogue.' Biochem J 238(2): 419-24. Wilkinson, T. C. and D. C. Wilton (1987). 'Studies on fatty acid-binding proteins. The binding properties of rat liver fatty acid-binding protein.' Biochem J 247(2): 485-8. Wilton, D. C. (1990). 'The fatty acid analogue 11-(dansylamino)undecanoic acid is a fluorescent probe for the bilirubin-binding sites of albumin and not for the high-affinity fatty acid-binding sites.' Biochem J 270(1): 163-6. Wu, W. T., C. N. Chen, et al. (2005). 'Lysophospholipids enhance matrix metalloproteinase-2 expression in human endothelial cells.' Endocrinology 146(8): 3387-400. Xiao, S. J., M. Wieland, et al. (2005). 'Surface reactions of 4-aminothiophenol with heterobifunctional crosslinkers bearing both succinimidyl ester and maleimide for biomolecular immobilization.' J Colloid Interface Sci 290(1): 172-83. Xie, Y., T. C. Gibbs, et al. (2002). 'Role for 18:1 lysophosphatidic acid as an autocrine mediator in prostate cancer cells.' J Biol Chem 277(36): 32516-26. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25634 | - |
| dc.description.abstract | 摘要
水解磷脂質是一種構造十分簡單的磷脂質。由於水解磷脂質是訊息傳遞系統中相當重要的調節因子，因此與許多生理功能密切相關。水解磷脂質在人體中存在於血清、腦髓液及其他體液；在體內循環時則多數與血清白蛋白相結合。由於在許多研究中發現，部分疾病患者的血清水解磷脂質含量較正常個體有顯著的增加，因此關於水解磷脂質與其主要結合之蛋白質 – 血清白蛋白間之互動關係之研究，對於未來針對水解磷脂質之親和材料的發展與設計自有其貢獻。然而在過去的研究中，多數的研究著重在水解磷脂質其生物性功能的研究，關於水解磷脂質與血清白蛋白之互動關係的研究較為少見，同時此類研究多只專注於兩者靜態親和關係與其他結合位競爭脂質的比較。在本研究中，利用可行即時動態監測的生物感測器 – 石英晶體微天平，於金電極上所固定之自組裝白蛋白單層膜偵測白蛋白對水解磷脂質的動態吸附行為。並配合phosphate capture complex加重水解磷脂質，以增加偵測之靈敏度。 | zh_TW |
| dc.description.abstract | Abstract
Lysophosphatidic acid (LPA) is a simple structure phospholipid. It plays a crucial role in signaling pathway which is related to numerous drown stream biological functions. LPA presents in serum, saliva, and other body fluids. In circulation, most LPA binds to serum albumin. Because of the notable increasing LPA concentration in clients with cancers, renal disease et al., the need to eliminate the excessive LPA in those populations is apparent. To understand the interaction relation between LPA and its carrier – serum albumin can contribute to the development of the bioaffinity materials to remove the LPA from circulation. However, the previous researches solely aim at the static affinity properties; the dynamic information remains unknown. In this study, we use a real time monitoring device – quartz crystal microbalance, to observe the dynamic adsorption behavior of LPA onto a self assembly albumin monolayer. Moreover, we combine the phosphate capture complex to weight the LPA and thus, increase the detection sensitivity of LPA. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T06:22:17Z (GMT). No. of bitstreams: 1 ntu-95-R93548048-1.pdf: 1391398 bytes, checksum: 1c74a7a99f1416c98d58d0383e9fbaf8 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | Contents
摘要 3 Abstract 4 1. Introduction 1.1 Research background & Literature review 5 1.1.1 Lysophosphatidic acid 5 1.1.2 Phosphate capture molecule 1.1.3 Biosensor 10 1.1.3.1 Principle of biosensors 10 1.1.3.2 Classification of biosensors 11 1.1.3.3 Introduction of quartz crystal microbalance 14 1.2 Purpose 18 2. Materials and methods 2.1 Materials 20 2.1.1 Lysophosphatidic acid 20 2.1.2 Phosphate capture molecule 20 2.1.3 Quartz crystal microbalance 21 2.1.3.1 Apparatus 21 2.1.3.2 Electrons fabrication 24 2.2 Method 2.2.1 Experiment design 24 2.2.2 Procedure 26 2.2.2.1 Fabricate the albumin immobilized QCM chips 26 2.2.2.1.1 The comparison of surface clean methods 26 2.2.2.1.2 The surface immobilized methods 26 2. 2.2.1.3 Experimental Procedure 27 2.2.2.2 Prepare of the LPA solution 28 2.2.2.3 Use FIA system to introduce the LPA solution 28 2.2.2.4 Synthesize the phosphate capture molecule 29 3. Result 3.1 QCM detection result 3.1.1 Albumin monolayer generation 31 3.1.1.1 QCM frequency shift after treating of every steps 31 3.1.1.2 Morphology information 31 3.1.1.3 The QCM frequency diversity of the treated electrodes 32 3.1.2 Subjects adsorption onto the albumin monolayer 33 3.1.2.1 The QCM frequency decrease due to the acquired subjects. 33 3.1.2.2 The real time monitoring of the adsorption 34 3.2 Phosphate capture molecule 35 3.3 Combine the phosphate capture complex to weight LPA 38 3.4 The other parameters which influence the adsorption 39 3.4.1 The pH value 40 3.4.2 The LPA concentration 40 4. Conclusion & Future work 41 References 42 | |
| dc.language.iso | en | |
| dc.title | 應用石英晶體微天平於水解磷脂質在白蛋白自組裝單層膜上吸附行為之研究 | zh_TW |
| dc.title | A Study on Adsorption Behavior of Lysophosphatidic Acid on Albumin Self Assemble Monolayer using Quartz Crystal Microbalance | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鍾次文,劉得任,梁有志 | |
| dc.subject.keyword | 水解磷脂質,石英晶體微天平, | zh_TW |
| dc.subject.keyword | Lysophosphatidic acid,Quartz crystal microbalance, | en |
| dc.relation.page | 43 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2006-07-31 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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