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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 費昌勇(Chang-Young Fei) | |
| dc.contributor.author | Chao-Chun Cheng | en |
| dc.contributor.author | 鄭兆君 | zh_TW |
| dc.date.accessioned | 2021-06-08T06:17:18Z | - |
| dc.date.copyright | 2007-02-02 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-01-25 | |
| dc.identifier.citation | 1.杜文圓。蠕蟲。醫用寄生蟲學手冊。286-287，2003。
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Parasitol 117: 73-83, 2003. 12.Bazzocchi C, Jamnongluk W, O’Neill SL, Anderson Tim JC, Genchi C, Bandi C. wsp Gene Sequences from the Wolbachia of Filarial Nematodes. Curr. Opin. Microbiol. 41: 96-100, 2000. 13.Bowman DD. Phylum Nematoda, Georgis’ Parasitology for Veterinarians. 8th ed., W.B. Saunders Company, USA. 216-222, 1999. 14.Bowman DD, Hendrix CM, Lindsay DS, Barr SC. The Nematodes, Feline Clinical Parasitology. A Blackwell Science Company, USA. 331-334, 2002. 15.Brunner CJ, Hendrix CM, Blagburn BL, Hanralan LA. Comparison of serologic tests for detection of antigen in canine heartworm infection. JAVMA 192: 1423-1427, 1988. ( 摘自楊， 2000 ) 16.Cross HF, Haarbrink M, Egerton G, Yazdanbakhsh M, Taylor MJ. Severe reaction to filarial chemotherapy and release of Wolbachia endosymbionts into blood. Lancet. 385: 1873-1875, 2001. 17.Foster JM, Kumar S, Ganatra MB, Kamal IH, Ware J, Ingram J, Pope-Chappell J, Guiliano D, Whitton C, Daub J, Blaxter ML, Slatko BE. Construction of bacterial artificial chromosome libraies from the parasitic nematode Brugia malayi and physical mapping of the genome of its Wolbachia endosymbiont. Parasitol. 34: 733-746, 2004. 18.Gillis JM, Smith RD, Todd KS. Diagnostic criteria for an anzyme-linked immunosorbent assay for occult heartworm disease: Standardizatuon of the test system in naturally exposed dogs. Am J Vet Res 45: 2289-2292, 1984. 19.Giordano R, Jackson JJ, Robertson HM. The role of Wolbachia bacteria in reproductive incompatilities and hybrid zones of Diabrotica beetles and Gryllus crickets. Proc. Natl. Acad. Sci. USA 94: 11439-44, 1995. 20.Grieve RB, Lok JB, Glickman LT. Epidemiology of canine heartworm infection. Epidemiol Rev 5: 220-246, 1983. 21.Herting M.The rickettsia, Wolbachia pipiens ( gen. et sp. n. ) and assosiatedn inclusions of the mosquito, Culex pipiens. Parasitology 28: 453-486, 1936. (摘自 Stouthamer R. 1999.) 22.Johanowicz DL, Hoy MA. Experimental induction and termination of nonreciprocal reproductive incompatilities in a parahaploid mite. Entomol. Exp. Appl. 87: 51-58, 1998. 23.Kramer LH, Passeri B, Corona S, Simoncini L. Immunohistochemical/ immunogold detection and distribution of the endosymbiont Wolbachia of Dirofilaria immitis and Brugia pahangi using a polyclonal antiserum rasied against WSP ( Wolbachia surface protein ). Parasitol Res. 89: 381-386, 2004. 24.Mar PH, Yang IC, Chang GN, Andrew Fei CY. Specific polymerase chain reaction for differential diagnosis of Dirofilaria immitis and Dipetalonema reconditum using primers derived from internal transcribed spacer region 2 (ITS2). Vet. Parasitol. 106: 243-252, 2002. 25.Marcos-Atxutegi C, Kramer LH, Fernandez I, Simoncini L, Genchi M, Prieto G., Simón F. Th1 response in BALB/c mice immunized with Dirofilaria immitis soluble antigens: a possible role for Wolbachia? Vet. Parasitol. 112: 117-130, 2003. 26.McGraw EA, O’Neill SL. Wolbachia pipientis: intercellular infection and pathogenesis in Drosophila, Curr. Opin. Microbiol. 7: 67-70, 2004. 27.Morchón R, Ferreria AC, Martín-Pacho JR, Montoya A, Mortarino M, Genchi C, Simón F. Specific IgG antibody response antigens of Dirofilaria immitis and its Wolbachia endosymbiont bacterium in cats with natural and experimental infections. Vet. Parasitol. 125: 313-321, 2004. 28.Muro A, Genchi C, Cordero M, Simon F. Human dirofilariasis in the European Union. Parasitol Today 15: 286-389, 1999. 29.O’Nell SL, Hoffman AA, Werren JH,eds. Influential Passenger: Inherited Microorganisms and Arthropod Reproduction, New York: Oxford Univ. Press, 1-214, 1997. ( 摘自 Stouthamer R. 1999. ) 30.Owen J, Slocombe D. Heartworm in dogs. http://www.heartwormsociety.org/ 美國心絲蟲協會 31.Simón F, Prieto G., Morchón R, Bazzocchi C, Bandi C, Genchi C. Immunoglobulin G Antibodies against the Endosymbionts of Filarial Nematodes（ Wolbachia ）in Patients with Pulmonary Dirofilariasis, Am. Soc. Microbiol .180-181, 2003. 32.Snyder PS, Levy JK, Salute ME, Gorman SP, Kubilis PS, Smail PW, George LL. Performance of serologic tests used to detect heartworm infection in cats. J Am Vet Med Assoc. 216: 693-700, 2000. 33.Stouthamer R, Breeuwer JAJ, Hurst GDD. Wolbachia Pipientis: Microbial Mainpulator of Arthropod Reproduction. Annu. Rev. Microbiol. 53: 71-102, 1999. 34.Taylor MJ, Helen FC, Bilo K. Inflammatory Responses Induced by the Filarial Nematode Brugia malayi Are Mediated by Lipopolysaccharide-like Activity from Endosymbiontic Wolbachia bacteria. J. Exp. Med. 191: 1429-1435, 2000. 35.Taylor MJ, Hoerauf. Wolbachia Bacteria of Filarial Nematodes.Parasitol. Today. 15: 437-442, 1999. 36.Tilly LP, Goodwin JK. Canine Heartworm Disease, Manual of Canine and Feline Cardiology. 3rd ed., W.B. Saunders Company, USA, 215-249, 2001. 37.Wang LC. Canine filarial infections in north Taiwan. Acta Trop 68: 115-120, 1997. 38.Wang LC. Comparison of whole-blood agglutination test and an ELISA for the dection og the antigens of Dirifilaria immitis in dogs. Ann Trop Med Parasitol 92: 73-77, 1998. 39.Werren JH, Windsor D, Guo L. Distribution of Wolbachia among neotropical arthropods. Proc. R. Soc. London Ser. B. 262: 197-204,1995. 40.Wu CC, Fan PC. Pervalence of canine dirofilariasis in Taiwan. J Helminthol 77: 83-88, 2003. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25527 | - |
| dc.description.abstract | 犬心絲蟲（ Dirofilaria immitis ）為一種寄生在狗、貓、狐、狼心臟及肺動脈的寄生蟲疾病。屬於 Filaroididae 科的 Dirofilaria 屬，往往造成感染動物的臨床症狀且影響其心臟功能。
目前犬心絲蟲的判定為臨床症狀、血液濕抹片中見到microfilaria的出現，加上商品化的ELISA 試劑（enzyme-linked immunosorbent assay）偵測。狗為 D.immitis 的固有宿主，可由以上方法得知心絲蟲的感染與否。在貓，大部份的貓在表皮血液中幾乎看不到幼絲蟲，但可能有具代表性的放射線學檢查結果或陽性的心絲蟲抗原 ELISA 試驗結果。 由於貓是偶然宿主, 故感染的蟲體數量很少 ， 所呈現的抗原量相對也低 ， 所以我們很可能會忽略貓的心絲蟲感染症 。在貓感染D.immitis 在血中見到 microfilaria 的機會很低，加上貓感染 D.immitis 只要幾隻成蟲寄生即可造成死亡。 Wolbachia spp.為一種垂直傳播的絕對細胞內寄生性立克次體，寄生在節肢動物及絲蟲體上，而它在絲蟲症上扮演著十分重要的角色。 D. immitis 蟲體上帶有 Wol. spp. ，兩者之間的關係目前仍有許多人探討。 本研究的目的在於利用聚合酶鍊鎖反應（ polymerase-chain-reaction；PCR ）之檢測 , 以利於日後建立犬、貓心絲蟲感染症在臨床上的診斷方法。 並於建立診斷方法後調查犬、貓感染 D. immitis 的盛行率以及探討 D. immitis 上存有Wol. spp. 的比率 。 | zh_TW |
| dc.description.abstract | Abstract
The heartworm, Dirofilaria immitis, is a mosquito-borne pathogenic parasite of canids. It has also been found in other animals, such as seals, raccoons, as well as cats. The adult worms normally habitat in the right ventricle and pulmonary arteries. Cats usually tend to harbor very few and unisex adult worms and thus usually to remain amicrofilaremic. Additionally, cats usually fall victims to sudden death as a result of aberrantly migrating heatworms. Consequently, there is hardly reliable diagnostic method capable to identify the status of infection. Wolbachia is an obligate endosymbiont bacteria harbored by many arthropods and filarial worms, including D. immitis. The first description of Wol. spp. was made in 1924, when it was detected in the ovaries of the mosquito Culex pipiens and classified as an unnamed Rickettsia. All isolates of filarial parasites studied so far by PCR are infected with this bacteria. We develop nucleotide diagnostic technique of dirofilariasis in cat and dog by study the relationships between Wol. spp. and D. immitis . The purpose of the study contain: (1) Prevalance survey of cat and dog dirofilariasis. (2) Try to construct a clinical diagnostic technique of D.immitis infection in cat by PCR. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T06:17:18Z (GMT). No. of bitstreams: 1 ntu-96-R93629015-1.pdf: 1646759 bytes, checksum: 1f61e765afd1a3cc587f8f775ebee01b (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目錄…………………………………………………………………………………..Ⅰ
圖次…………………………………………………………………………………..Ⅲ 表次…………………………………………………………………………………..Ⅳ 中文摘要………………………………………………………………………………1 英文摘要………………………………………………………………………………2 前言……………………………………………………………………………………4 第一章 文獻回顧 第一節 Dirofilaria immitis 簡介……………………………………..6 第二節 Wolbachia spp. 簡介……………………………………….10 第三節 Dirofilaria immitis及 Wolbachia 診斷方法………………16 第二章 試驗設計……………………………………………………………………19 第三章 材料與方法 第一節 血液抹片之製作及鏡撿……………………………………21 第二節 蟲體之蒐集及透化…………………………………………23 第三節 Dirofilaria immitis ELISA套組…………………………….24 第四節 DNA 之萃取……………………………………………….26 第五節 PCR技術之建立……………………………………………28 第六節 PCR 特異性試驗…………………………………………..32 第七節 瓊脂膠體電泳………………………………………………33 第四章 結果 第一節 蟲體之蒐集………………………………………………..35 第二節 檢驗套組之結果…………………………………………..36 第三節 PCR 結果………………………………………………..37 第四節 引子之特異性試驗………………………………………..38 第五節 核酸定序結果……………………………………………..39 第五章 討論 第一節 試驗設計……………………………………………………40 第二節 PCR 反應條件……………………………………………..41 第三節 引子之特異性………………………………………………43 第四節 核酸序列之比對……………………………………………44 第五節 臨床應用……………………………………………………45 結論與未來展望……………………………………………………………………..46 參考文獻 附錄…………………………………………………………………………………..52 圖次 圖 1. a. 心絲蟲生活史 b. 肺組織切片 …………………………………………………………….52 圖 2. 犬檢驗套組之判讀與貓檢驗套組的陰性結果……………………………...53 圖3. 透化技術區分心絲蟲成蟲公母……………………………………………..54 圖4. 免疫組織化學染色 ……………………………………………………….55 圖 5. 電子顯微鏡切片……………………………………………………………..56 圖 6.以 Wol-0218 引子跑出之心絲蟲成蟲結果………………………………….57 圖 7.以 Wol-0218 引子跑出之犬血第一次 PCR 結果………………………….58 圖 8.以 2Wol-0218 引子跑出之犬血巢式 PCR 結果…………………………...59 圖 9.以 2Wol-0218 引子跑出之 A 貓血液結果…………………………………60 圖 10.以 2Wol-0218 引子跑出之 A 貓腹水結果………………………………..61 圖 11.以 2Wol-0218 引子跑出之 B 貓血液結果 ……………………………….62 圖 12.以 2Wol-0218 引子跑出之 2 ( A, B ) 隻貓血液結果……………………..63 圖 13.以 Ftsz 引子跑出之心絲蟲成蟲結果………………………………………64 圖 14.以 Ftsz 引子跑出之 2 ( A, B ) 隻貓血液結果…………………………….65 圖 15.以 ITS-2 引子跑 Wolbachia 陽性的貓血液……………………………….66 圖 16. ftsz 基因序列………………………………………………………………...67 圖 17. 貓血液定序後上網比對 ftsz 基因序列 …………………………………..69 圖 18. Ehrlichia spp. 及 Wolbachia spp. 序列之 blast 結果…………………..71 圖 19. 引子之特異性試驗: 以 2Wol-0218 引子跑出之血液結果……………..75 圖 20.引子之特異性試驗: 以 Ftsz 引子跑出之血液結果……………………….76 表次 表1. 貓心絲蟲的臨床症狀…………………………………………………………77 表2. 貓抗原與抗體檢測之比較……………………………………………………78 表 3. Wolbachia 在各種絲蟲體上以 PCR、電子顯微鏡、免疫組織化學染色的結果…………………………………………………………79 表 4. 犬、貓心絲蟲之比較……………………………………………………80 表 5. 以 PCR、 ELISA kit 、Blood smear 、放射線學等方式診斷心絲蟲感染之比較…………………………………………………………..81 | |
| dc.language.iso | zh-TW | |
| dc.subject | 引子 | zh_TW |
| dc.subject | 心絲蟲 | zh_TW |
| dc.subject | wolbachia spp. | en |
| dc.subject | heartworm disease | en |
| dc.subject | D. immitis | en |
| dc.title | 利用Wolbachia spp. 之特異性引子建立犬、貓心絲蟲症( Canine and Feline heartworm disease)感染之核酸診斷技術 | zh_TW |
| dc.title | A study of the specific polymerase chain reaction for dirofilariasis in dogs and cats by using specific primers of Wolbachia spp. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林大盛(Dah-Sheng Lin),蘇耀期(Yao-Chi Shu) | |
| dc.subject.keyword | 心絲蟲,引子, | zh_TW |
| dc.subject.keyword | wolbachia spp.,D. immitis,heartworm disease, | en |
| dc.relation.page | 81 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2007-01-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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