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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 湯志永(Chih-Yung Tang) | |
dc.contributor.author | Cheng-Hsin Liu | en |
dc.contributor.author | 劉政昕 | zh_TW |
dc.date.accessioned | 2021-06-08T06:16:53Z | - |
dc.date.copyright | 2011-10-05 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-03 | |
dc.identifier.citation | Ahmed N, Ramjeesingh M, Wong S, Varga A, Garami E & Bear CE. (2000). Chloride channel activity of CIC-2 is modified by the actin cytoskeleton. Biochemical Journal (352), 789-794.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25519 | - |
dc.description.abstract | CLC2氯離子通道廣泛地分布在各種組織,並且會受到過極化電位、細胞膨脹、細胞外微酸環境以及細胞內氯離子濃度增加而活化。近來研究發現,CLC2在大鼠海馬迴的錐狀細胞中,具有協助胞內氯離子向外排除之作用。此外,CLC2被推測在老年小鼠的神經保護機制中可能扮演角色。然而CLC2在神經細胞中確切的分子調控機轉仍有待釐清。因此,本篇論文的研究方向在尋找大鼠腦中與CLC2有交互作用之蛋白質,並探討這些蛋白質如何影響CLC2,進而對CLC2的基本性質與生理角色有更深入的了解。
本實驗室過去利用酵母菌雙雜交技術,篩選出大鼠腦部cDNA library中幾個可能與CLC2具有交互作用的蛋白質,本研究更進一步利用共同免疫沉澱法與GST pull-down確認其交互作用關係。結果顯示其中的四個蛋白質與CLC2具有交互作用。我們由免疫螢光染色實驗得知,共同表現交互作用蛋白質並不會影響CLC2在細胞內的分佈位置。然而我們目前尚無法確定交互作用蛋白質是否會影響CLC2總表現量,因此需要日後的實驗結果佐證。未來我們將利用電生理技術以及其他生化實驗方法,分析交互作用蛋白在CLC2的生物物理特性與細胞內的生合成所扮演之角色。 | zh_TW |
dc.description.abstract | CLC2 channel is a ubiquitously expressed voltage-gated chloride channel that is activated by hyperpolarization, cell swelling, extracellular acidification, and rise of intracellular chloride concentration. In recent studies, CLC2 has been suggested to assist chloride extrusion in hippocampal pyramidal cells. In addition, CLC2 has been implicated to have neuroprotective roles in aging mice. However, the precise cellular regulatory properties of CLC2 channel in neurons remain elusive. Therefore, we aimed to understand the physiological roles and cellular signaling mechanisms of CLC2 channels by searching for novel CLC2-interacting proteins.
Previous studies in our lab utilizing yeast-two hybrid screening of rat brain cDNA library has identified several potential CLC2-interacting proteins. In this study, we further confirmed these interactions by using co-immunoprecipitation and GST pull-down assay. Our data suggest that four of these candidate proteins display significant interaction with CLC2. Immunofluorescence analysis revealed that these candidate proteins failed to affect the subcellular localization of CLC2 in HEK293T cells. It is still unclear whether total CLC2 expression may be affected in the presence of candidate proteins and thus required further studies. In the future, we plan to apply electrophysiological and biochemical assays to determine whether these interacting candidates can affect the biophysical and biosynthetic properties of CLC2 channels. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T06:16:53Z (GMT). No. of bitstreams: 1 ntu-100-R98441001-1.pdf: 2005425 bytes, checksum: a2e6fd03e954c8f48a8748f4d6e428ad (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iii 圖次 vi 緒論(Introduction) 1 1. CLC氯離子通道 1 2. CLC2氯離子通道 2 3. 影響CLC2功能的分子以及交互作用蛋白 5 4. 以酵母菌雙雜交實驗尋找可能與CLC2有交互作用之蛋白質 6 5. 研究目的 12 材料與方法(Materials and Methods) 13 1. Molecular biology 13 1.1 CLC2相關plasmid製備 13 1.2 GST-CLC2 plasmid製備 13 1.3 Candidate protein製備 14 2. Protein Biochemistry 15 2.1 將plasmid DNA轉殖進入HEK293T細胞 15 2.2 共同免疫沉降法 (Co-Immunoprecipitation, Co-IP) 15 2.3 GST pull-down 17 2.4 Biotinylation 18 2.5免疫螢光染色(Immunofluorescence microscopy) 19 2.6 西方墨點法 (Western blotting) 20 2.7 資料分析 20 結果(Results) 21 1.以西方墨點法確認CLC2以及可能交互作用蛋白質在HEK293T細胞中之表現 21 2.檢驗CLC2與可能交互作用蛋白之間交互作用情形 21 2.1 以GST pull-down assay以及共同免疫沉降法 (Co-Immunoprecipitation)檢視10個交互作用蛋白質與CLC2之交互作用情形 22 2.2以共同免疫沉降法 (Co-Immunoprecipitation)檢視小鼠腦中CLC2與NSF之間交互作用關係 23 3.觀察具交互作用之蛋白質對CLC2在細胞中分佈位置的影響 24 4.檢視具交互作用之蛋白質對CLC2總表現量的影響 25 討論(Discussions) 27 1. 蛋白質交互作用實驗系統的比較 27 2. NSF與CLC2交互作用的探討 28 3. SMAD1與CLC2交互作用的探討 29 4. LRRC46與CLC2交互作用的探討 30 5. Calpain2與CLC2交互作用的探討 31 6. 其他蛋白質與CLC2交互作用的探討 31 7. CLC2與蛋白質的交互作用區域 32 8. 待解決之問題與未來目標 32 結論(Conclusion) 34 參考資料(References) 35 圖表(Figures and Tables) 47 表一. 實驗中所使用的primer列表 47 圖一. Flag-CLC2以及十種可能交互作用蛋白在HEK293T細胞的表現 48 圖二. 分次純化GST與GST-CLC2-C2蛋白以及與glutathione beads結合量判定 49 圖三. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-NSF之間互作用情形 50 圖四. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-SMAD1之間交互作用情形 51 圖五. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-LRRC46之間交互作用情形 52 圖六. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-Calpain2之間交互作用情形 53 圖七. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-U1snRNP A之間交互作用情形 54 圖八. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC與Myc-Carhsp1之間交互作用情形 55 圖九. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-Stip1之間交互作用情形 56 圖十. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-MPP8之間交互作用情形 57 圖十一. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-CD2BP2之間交互作用情形 58 圖十二. 以GST pull-down assay與免疫沉降法(Co-IP)檢視CLC2與Myc-Calmodulin之間交互作用情形 59 圖十三. 以免疫沉降法(Co-IP)檢視小鼠腦部中CLC2與NSF之交互作用關係 60 圖十四. 以免疫螢光染色(immunofluorescence)觀察Flag-CLC2在HEK293T細胞中的分佈情形 61 圖十五. 以免疫螢光染色(immunofluorescence)觀察Myc-NSF以及共同表現Flag-CLC2後兩者在HEK293T細胞中的分佈情形 62 圖十六. 以免疫螢光染色(immunofluorescence)觀察Myc-SMAD1以及共同表現Flag-CLC2後兩者在HEK293T細胞中的分佈情形 63 圖十七. 以免疫螢光染色(immunofluorescence)觀察Myc-LRRC46以及共同表現Flag-CLC2後兩者在HEK293T細胞中的分佈情形 64 圖十八. 以免疫螢光染色(immunofluorescence)觀察Myc-Calpain2以及共同表現Flag-CLC2後兩者在HEK293T細胞中的分佈情形 65 圖十九. 觀察以1:1比例共同表現Flag-CLC2與交互作用蛋白質,對Flag-CLC2的總表現量影響 66 附表一. 以Yeast two hybrid篩選到在大鼠腦部cDNA library中可能與CLC2有交 互作用之蛋白質 67 附表二. 以X-gal test與leucine-requirement test,檢視篩選出的可能交互作用蛋白質與ClC-2在酵母菌中的交互作用關係 68 | |
dc.language.iso | zh-TW | |
dc.title | CLC2氯離子通道交互作用蛋白質之鑑定 | zh_TW |
dc.title | Characterization of Putative CLC2 Interacting Proteins | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鄭瓊娟(Chung-Jiuan Jeng),王淑美(Seu-Mei Wang),繆希椿(Shi-Chuen Miaw) | |
dc.subject.keyword | 氯離子通道,交互作用蛋白質, | zh_TW |
dc.subject.keyword | CLC2,interacting proteins, | en |
dc.relation.page | 68 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2011-08-03 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 生理學研究所 | zh_TW |
顯示於系所單位: | 生理學科所 |
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