請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25332完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭石通 | |
| dc.contributor.author | An-Shan Hsiao | en |
| dc.contributor.author | 蕭安珊 | zh_TW |
| dc.date.accessioned | 2021-06-08T06:09:22Z | - |
| dc.date.copyright | 2007-07-19 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-12 | |
| dc.identifier.citation | 林耀輝（1994）我所了解的甘藷。科學農業 25：357-360
藍逸青（1999）利用轉殖菸草分析甘藷sporamin 啟動子之受傷誘導調控機制。國立台灣大學植物學研究所碩士論文。 曾博文 (2000) 甘藷傷害誘導基因Ipomoelin的表現與性質測定。國立台灣大學植物學研究所碩士論文。 邱宜芳 (2003) 甘薯傷害誘導之蛋白質Ipomoelin的功能測定。國立台灣大學植物學研究所碩士論文。 陳玉琪 (2003) 甘薯Ipomoelin基因之定性及調控。國立台灣大學植物學研究所博士論文。 李雨霖（2005）甘藷Ipomoelin 啟動子特性分析。國立台灣大學植物學研究所碩士論文。 李培芬等（2006）台灣的自然資源與生態資料庫.III農林漁牧。行政院農業委員會林務局。 Arimura Gen-ichiro, Kost C., Boland W. (2005) Herbivore-induced, indirect plant defences. Biochem. Biophys. Acta. 1734: 91-111. Baker, C. J., Orlandi, E. W., (1995) Active oxygen in plant/pathogen interactions. Annu. Rev. Phytopathol. 33 : 299-321. Barber, M. S., Bertram, R. E., Ride, J. P. (1989) Chitin oligosaccharides elicit lignification in wounded wheat leaves. Physiol. Mol. Plant Pathol. 34: 3-12. Bergey D. R., Orozco-Cardenas M, De Moura D. S., and Ryan C.A. (1999) A wound- and systemin-inducible polygalacturonase in tomato leaves. Proc. Natl. Acad. Sci. USA 96 : 1756-1760. Biondi S., Scaramagli S., Capitani F., Altamura M. M., Torrigiani P. (2001) Methyl jasmonate upregulates biosynthetic gene expression, oxidation and conjugation of polyamines, and inhibits shoot formation in tobacco thin layers. J. Exp. Bot. 52: 231-242. Birnboim, H. C., and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7: 1513-1518. Bradley, D. J., Kjellbom, P., and Lamb, C. J. (1992) Elicitor- and wound- induced oxidative cross-linking of a praline-rich plant cell wall protein: a novel, rapid defense response. Cell 70 : 21-30. Brisson, L. F., Tenhaken, R., Lamb, C, J. (1994) Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance. Plant Cell 6 : 1703-1712. Chabannes M., Barakate A., Lapierre C., Marita J. M., Ralph J., Pean M., Danoun S., Halpin C., Grima-Pettenati J., Boudet A. M. (2001) Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants. Plant J. 28: 257-270. Chang C., Bleecker A. B. (2004) Ethylene biology. More than a gas. Plant Physiol. 136: 2895-2899. Cronje M. J., Snyman M., Bornman L., Weir I. E. (2003) A rapid and reliable flow cytometric method for determining Hsp70 levels in tobacco protoplasts. Methods Cell Sci. 25: 237-246. Cruz Castillo M., Martinez C., Buchala A., Metraux J. P., Leon J. (2004) Gene-specific involvement of beta-oxidation in wound-activated responses in Arabidopsis. Plant Physiol. 135: 85-94. Delledonne, M., Xia, Y., Dixon, R. A. and Lamb, C. (1998) Nitric oxide functions as a signal in plant disease resistance. Nature (London) 394 : 585-588. Devoto A., Turner J. G. (2001) Regulation of jasmonate-mediated plant responses in arabidopsis. Ann. Bot. (Lond) 92: 329-337. Dixon, R. A. and Harrison, M. J. (1990a) Activation, structure and organization of genes involved in microbial defense in plants. Adv Genet 28 : 165-234. Dixon, R.A. and Lamb, C. J. (1990b) Molecular communication on interactions between plants and microbial pathogens. Annu Rev Plant Physiol. Plant Mol. Biol. 41 : 339-367. Doares, S. H., Narváez-Vásquez, J., Conconi, A. and Ryan, C. A. (1995) Salicylic acid inhibits synthesis of proteinase inhibitors in tomato leaves induced by systemin and jasmonic acid. Plant Physiol. 108: 1741-1746. Durrant W. E., Dong X. (2004) Systemic acquired resistance. Annu. Rev. Phytopathol. 42: 185-209. Etheridge N., Chen Y. F, Schaller G. E. (2005) Dissecting the ethylene pathway of Arabidopsis. Brief Funct Genomic Proteomic. 3: 372-381. Eulgem, T., Rushton, P.J., Schmelzer, E., Hahlbrock, K., and Somssich, I.E. (1999). Early nuclear events in plant defense: Rapid gene activation by WRKY transcription factors. EMBO J. 18: 4689–4699. Farmer E. E., Almeras E., Krishnamurthy V. (2003) Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Cur.r Opin. Plant Biol. 6: 372-378. Franco O. L., Rigden D. J., Melo F. R., Grossi-De-Sa M. F. (2002) Plant alpha-amylase inhibitors and their interaction with insect alpha-amylases. Eur. J. Biochem. 269: 397-412. Gheysen, G., Eycken, W. van der, Barthels, N., Karimi, M., Montagu, M. van (1996) The exploitation of nematode-responsive plant genes in novel nematode control methods. Pestic Sci. 47 : 95-101. Giovannoni J. J., Yagi K., Kobayashi Y., Howe G. A. (2005) Role of {beta}-oxidation in jasmonate biosynthesis and systemic wound signaling in tomato. Plant Cell 17: 971-986. Goddijn, O. J. M., Lindsey, K. van der, Lee, F. M., Klap, J. C. And Gheysen, G. Med. Rac. Landbouww. (1992) Unit. Gent 57/3a: 895. Gruden K., Kuipers A. G., Guncar G., Slapar N., Strukelj B., Jongsma M. A. (2004) Molecular basis of Colorado potato beetle adaptation to potato plant defence at the level of digestive cysteine proteinases. Insect Biochem. Mol. Biol. 34: 365-375. Guerineau F., Benjdia M., Zhou D. X. (2003) A jasmonate-responsive element within the A. thaliana vsp1 promoter. J. Exp. Bot. 54: 1153-1162. Guilley H., Dudley R.K., Jonard G., Balazs E. and Richards K.E. (1982). Transcription of Cauliflower Mosaic Virus DNA: Detection of promoter sequences, and characterization of transcripts. Cell: 30:763-773. Guo H., Ecker J. R. (2004) The ethylene signaling pathway: new insights. Curr. Opin. Plant Biol. 7: 40-49. Hahlbrock, K. and Scheel, D. (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol Plant Mol Biol. 40 : 347-369. Himmelbach A., Yang Y., Grill E. (2003) Relay and control of abscisic acid signaling. Curr. Opin. Plant Biol. 6: 470-479. Horsch, R. B., Fry, J. E., Hoffmann, N. L. E, D., Rogers, S. F. and Fraley, R. T. (1985) A simple and general method for transferring genes into plants. Science 227 : 1229-1231. Iwamoto M., Higo H., Higo K. (2004) Strong expression of the rice catalase gene CatB promoter in protoplasts and roots of both a monocot and dicots. Plant Physiol. Biochem. 42: 241-249. Ish-Horowicz, D. and Burke, J. F. (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res. 9: 2989-2998. Jefferson, R. A., Kavanagh, T. A., and Bevan, M. W. (1987) GUS fusions : -Glucuronidase as a sensitive and versatile gene fusion marker. EMBO J. 6 : 3901-3908. Jin, J.B., Kim, Y.A., Kim, S.J., Lee, S.H., Kim, D.H., Cheong, G.W., and Hwang, I. (2001). A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis. Plant Cell 13: 1511–1526. Klement, Z. (1982) Hypersensitivity. In MS Mount, GH lacy, eds, Phytopathogenic Prokaryotes. Academic Press, New York, pp149-177. Kiraly, Z., El-Zahaby, J., Galal, A., Abdou, S. And Adam, A. (1993) Drrect of oxygen free radicals on plant pathogenic bacteria and fungi and on some plant diseases. In G Mozsik, J emerit, J Feher, B Matkovics, A Vincze, eds, Oxygen Free Gadicals and Scavengers in the Natural Sciences. Akademiai Kiado, Budapest, pp9-19. Li C., Schilmiller A. L., Liu G., Lee G. I., Jayanty S., Sageman C., Vrebalov J., Liechti R., Farmer E. E. (2003) The jasmonate biochemical pathway. Sci. STKE. 7: CM18. Low, P. S., Merida, J. R. (1996) The oxidative burst in plant defence : function and signal transduction. Physiol Plant 96 : 532-542. Mehdy, M. C. (1994) Active oxygen species in plant defense against pathogens. Plant Physiol 105 : 467-472. Meyer, P. (1995) Understanding and controlling transgene expression. Transgenics 3 : 322-327. Mitsuhara I., Ugaki M., Hirochika H., Ohshima M., Murakami T., Gotoh Y., Katayose Y., Nakamura S., Honkura R., Nishimiya S., Ueno K., Mochizuki A., Tanimoto H., Tsugawa H., Otsuki Y., and Ohashi Y. (1996) Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol. 37: 49-59. Moon J., Salzman RA., Ahn JE., Koiwa H , Zhu-Salzman K. (2004) Transcriptional regulation in cowpea bruchid guts during adaptation to a plant defence protease inhibitor. Insect Mol. Biol. 13: 283-291. O'Donnell, P. J., Calvert C., Atzorn R., Wasternack C., Leyser H. M. O., and Bowles D. J. (1996) Ethylene as a signal mediating the wound response of tomato plants. Science 274: 1914-1917. Papadakis A. K., Roubelakis-Angelakis K. A. (2005) Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide. Planta 220: 826-837. Pearce G., Ryan C. A. (2003) Systemic signaling in tomato plants for defense against herbivores. Isolation and characterization of three novel defense-signaling glycopeptide hormones coded in a single precursor gene. J. Biol. Chem. 278: 30044-30050. Peng, M. and Kuc, J. (1992) Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks. Phytopathology 82 : 696-699. Rietz S., Holk A., Scherer G. F. (2004) Expression of the patatin-related phospholipase A gene AtPLA IIA in Arabidopsis thaliana is up-regulated by salicylic acid, wounding, ethylene, and iron and phosphate deficiency. Planta. 219: 743-753. Ringli C., Keller B., Ryser U. (2001) Glycine-rich proteins as structural components of plant cell walls. Cell Mol. Life Sci. 58: 1430-1441. Rushton, P.J., Reinstadler, A., Lipka, V., Lippok, B., and Somssich, I.E. (2002). Synthetic plant promoters containing defined regulatory elements provide novel insights into pathogen- and wound-induced signaling. Plant Cell 14: 749–762. Ryan C. A. (2000) The systemin signaling pathway: differential activation of plant defensive genes. Biochim. Biophys. Acta. 1477: 112-121. Ryan, C. A. and Pearce, G. (1998) Systemin: a polypeptide signal for plant defensive genes. Annual Review of Cell and Developmental Biology 14 : 1-17. Schilmiller A. L., Howe G. A. (2005) Systemic signaling in the wound response. Curr. Opin. Plant Biol. 8: 1-9. Stephen, L. C., Hannapel, D. J., Krell, S. L. and Shogren, D. R. (1996) Agrobacterium T-DNA mutation causes the loss of GUS expression in transgenic tobacco. Plant Cell Rep. 15 : 414-417. Strittmatter, G., Gheysen, G., Gianinazzi-Pearson, V., Hahn, K., Niebel, A., Rohde, W., and Tacke, E. (1996). Infections with various types of organisms stimulate transcription from a short promoter fragment of the potato gst1 gene. Mol. Plant-Microbe Interact. 9: 68–73. Tang Z., Sadka A., Morishige D. T., Mullet J. E. (2001). Homeodomain leucine zipper proteins bind to the phosphate response domain of the soybean VspB tripartite promoter. Plant Physiology 125: 797-809. van der Hoorn R. A., Jones J. D. (2004) The plant proteolytic machinery and its role in defence. Curr. Opin. Plant Biol. 7: 400-407. Walling L. L. (2000) The myriad plant responses to herbivores. J. Plant Growth Regul. 19: 195-216. Wasternack C., Hause B. (2000) Jasmonates and octadecanoids: signals in plant stress responses and development. Prog. Nucleic Acid Res. Mol. Biol. 72: 165-221. Wendehenne D., Durner J., Klessig D. F. (2004) Nitric oxide: a new player in plant signalling and defence responses. Curr. Opin. Plant Biol. 7:449-455. Wojdyla A. T. (2004) Chitosan (biochikol 020 PC) in the control of some ornamental foliage diseases. Commun. Agric. Appl. Biol. Sci. 69: 705-715. Wu, G., Shortt B. J., Lawrece, E. B., Elaine, E. B., Fitzaimmons, K. C. and Shah, D. M. (1995) Disease resistance conferred by expression of a gene encoding H2O2-generation glucose oxidase in transgenic potato plants. Plant Cell 7 : 1357-1368. Xu B. and Timko M. (2004) Methyl jasmonate induced expression of the tobacco putrescine N-methyltransferase genes requires both G-box and GCC-motif elements. Plant Mol.Biol. 55: 743-761. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25332 | - |
| dc.description.abstract | Ipomoelin (IPO) 是一個甘藷(Ipomoea batatas cv. Tainung 57)傷害誘導的防禦蛋白，IPO可以被機械性傷害、茉莉酸甲酯(Methyl Jasmonate)以及乙烯等多種植物逆境與訊息分子所誘導表現，所以是一個可以研究植物如何開啟防禦機制的良好材料。針對實驗室李雨霖學長所釣取的甘藷IPO 啟動子序列全長片段A (位置-1240∼-1)，以及其所建構的啟動子缺失片段B (-1136∼-1 )、 C (-836∼-1 )、D (-496∼-1)、和E (-239∼-1)，進行菸草原生質體暫時性表現，並且進一步建構缺失順向調控區的啟動子長度1232 bp (片段F)，片段F缺失 -1136 bp∼-836 bp之間順向調控序列(cis-acting element)的ethylene response element (ERE, -1107∼-1100）；又建構長度為1235 bp的G片段，缺失 -496 bp∼ -239 bp之間的TGACG-motif (-404~-400)。針對全長啟動子和啟動子缺失片段B、C、D和E，以及含有缺失順向調控序列的片段F與 G，在接上b-glucunonidase(GUS) gene後，分別進行菸草轉殖，分析IPO 啟動子受傷害誘導之機制。在菸草原生質體暫時性表現以及轉殖菸草的實驗中，皆可證實IPO的啟動子是可被茉莉酸甲酯和乙烯所誘導表現的，同時發現茉莉酸甲酯誘導IPO的啟動子表現的倍率比乙烯來得高，感受的時間也較為迅速。而比較不同片段的缺失序列更可以證實：IPO的啟動子感受乙烯誘導的區域為位於-1107 bp 與-1100 bp 之間的ERE；IPO的啟動子感受茉莉酸甲酯誘導的區域為位於-404 bp∼-400 bp 之間的TGACG-motif。且TGACG-motif具有雙重角色：在平時，不受藥劑誘導的情況下，是作為一負向調控者，抑制IPO啟動子，使IPO啟動子在正常的情況下不表現，受傷害才表現；而在處理茉莉酸甲酯的情況之下，TGACG-motif則作為IPO啟動子感受茉莉酸甲酯的調控區域。 | zh_TW |
| dc.description.abstract | Ipomoelin (IPO), a wound-inducible defense protein from sweet potato (Ipomoea batatas cv. Tainung 57), can be induced by wounding, Methyl Jasmonate (MeJA), and ethylene, and is an excellent material to study plant defense system. The full length of IPO promoter, fragment A (position -1240~-1), and its serial deletion fragments B (-1136~-1 ), C (-836~-1 )、D (-496~-1 ), and E (-239~-1 ) were isolated and constructed by Yu-Lin Li, and their tansient expression in tobacco protoplast has been proceeded. Furthermore, motif deletion fragment F, which has deletion of a ethylene response element (ERE, -1107~-1100 ) between fragments B and C and motif deletion fragment G, of which the length is 1235 bp and has deletion of TGACG motif between fragments D and E (-496~-239), were also analyzed.
With attachment to the b-glucunonidase(GUS) gene, the full length, serial deletion, and motif deletion promoters were transformed into tobacco, and their expressions stimulated by ethylene and MeJA were studied in the stable transformants. Both the experiments of tobacco transient expression and stable transformation proved the effects of both MeJa and ethylene upon the induction of IPO promoter. Also, ERE that is located between -1107~-1100 was responsible for the induction of ethylene, and TGACG motif positioned -496~-239 was involved in MeJA stimulation in IPO promoter. Also, the induction magnification of MeJA upon IPO promoter was higher than that of ethylene, and the induction response of MeJA to IPO promoter was faster than that of ethylene. Interestingly, TGACG-motif functioned as a negative cis-element repressing the activity of IPO promoter in the absence of MeJA, but upon the addition of MeJA TGACG-motif positively helped IPO promoter expression. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T06:09:22Z (GMT). No. of bitstreams: 1 ntu-96-R94b42005-1.pdf: 992491 bytes, checksum: 3a17c0da504100e090bdb65935d71c59 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目 錄
中文摘要………………………………………………………………………………2 英文摘要………………………………………………………………………………3 第一章、前言 第一節 植物的防禦機制………………………………………………………….6 第二節 植物防禦基因轉殖的啟動子…………………………………………….9 第三節 甘藷ipomoelin的簡介…………………………………………………10 第四節 實驗方向與目的 ……………………………………………………….13 第二章、材料與方法 第一節 實驗材料………………………………………………………………..14 第二節 質體的製備………………………………………………………………15 第三節 菸草原生質體中的暫時性表現 2-3.1 原生質體的製備………………………………………………….20 2-3.2 電擊法…………………………………………………………….21 2-3.3 GUS螢光活性分析………………………………………………22 2-3.4 luciferase活性分析………………….………………………22 第四節 農桿菌的轉型 2-4.1 農桿菌勝任細胞的製備…………………………………………25 2-4.2 農桿菌勝任細胞的轉型…………………………………………26 2-4.3 農桿菌質體的抽取……….………………………………………26 第五節 菸草之基因轉殖與鑑定 2-5.1 葉圓片轉殖法…………….………………………………………27 2-5.2 菸草基因組DNA的萃取….………………………………………29 2-5.3 聚合脢連鎖反應法鑑定轉殖菸草植株………………………….29 第六節 轉殖菸草中ipomoelin啟動子受傷誘導機制之分析…………………30 2-6.1 GUS 螢光活性分析……………………………….………………30 2-6.2 相關誘導劑處理………………………………….………………33 2-6.3 GUS 活性染色分析……………………………….………………34 第七節 純化與ipomoelin啟動子專一性結合之核蛋白 2-7.1 純化核蛋白……………………………………….………………35 2-7.2 純化專一性結合之核蛋白……………………….………………36 2-7.3 蛋白質SDS膠體電泳分析……………………….………………40 2-7.4 銀染蛋白法……………………………………….………………42 第三章、結果 第一節、ipomoelin啟動子全長之簡介……………………….……………….45 第二節、菸草原生質體中的暫時性表現……………………….……………….46 第三節、PROIPO::pBI101轉型至農桿菌與其鑑定…………………………….47 第四節、轉殖菸草的轉殖及其篩選與鑑定……………………………………..48 第五節、ipomoelin啟動子全長（PROIPOA::GUS）傷害誘導機制的分析…..49 第六節、ipomoelin啟動子各缺失片段之傷害誘導機制的分析………………50 第七節、純化與ipomoelin啟動子專一性結合之核蛋白………………………54 第四章、討論 第一節、菸草原生質體中的暫時性表現與菸草基因轉殖………………………56 第二節、轉殖菸草中PROIPO-GUS基因的表現情形…………………………….59 第三節、甘藷ipomoelin啟動子的特性…………………………………………60 第四節、甘藷ipomoelin訊息調控機制及其生理功能…………………………61 第五節、未來展望…………………………………………………………………63 第五章、參考文獻…………………………………………………………………..65 圖表………………………………………………………………………………….72 附錄………………………………………………………………………………….91 | |
| dc.language.iso | zh-TW | |
| dc.subject | 啟動子 | zh_TW |
| dc.subject | promoter | en |
| dc.title | 甘藷Ipomoelin啟動子功能性分析 | zh_TW |
| dc.title | Characterization of the Ipomoelin promoter from
sweet potato | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張自忠,王淑美,謝旭亮 | |
| dc.subject.keyword | 啟動子, | zh_TW |
| dc.subject.keyword | promoter, | en |
| dc.relation.page | 93 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2007-07-16 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 969.23 kB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。