多孔性幾丁聚醣微粒製備、分析及應用

Abstract

Abstract In this research, porous chitosan microparticles were prepared by using a emulsification/freeze-gelation method. The effects of various processes parameters were investigated. The BSA-loading capability of the porous chitosan microparticles and that of a commercial powder were compared. First, the optimum HLB value of the surfactant utilized in this method was found to be in the range of 8.6-11.0. The size of the porous chitosan microparticles became smaller as the HLB values of the different surfactants approached the optimum HLB value. These microparticles prepared by using different surfactants had different structures；some were dense porous and some were loose porous. Second, Span 20/Tween 21 was found to be the optimum combination of surfactants with the HLB value of 10. The microparticles had homogeneous and loose porous structure, and the particle size was 153.4 μm and C.V. was 33.9%. Third, in the emulsification system when the proportion of petroleumbenzin (mL) / 1 wt% chitosan solution (mL) / surfactants (g) was adjusted to 100/20/1.5, the particle yield was increased by 3.2 folds with size of 163.3 μm (C.V. = 37.3%). Fourth, this research also investigated the influence of freezing temperature. The water uptake capability of microparticles increased obviously when the freezing temperature decreased. The water uptake capability was about 2300% when the freezing temperature was -25℃, which was 5.5 folds of that of commercial powder. Finally, the application of the porous chitosan microparticles in BSA loading indicated that BSA loading was complete at low BSA concentration (＜0.8 mg/mL) and was 190 mg BSA/g chitosan at high BSA concentration (＞2.0 mg/mL). The loading capacity of these microparticles was 4 folds of that of commercial powder. This research demonstrated that the properties of the porous chitosan microparticles could be modulated by varying the process parameters for application in various fields.