請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24995完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 姚宗珍(Chung-Chen Yao) | |
| dc.contributor.author | Kuo-Jung Tseng | en |
| dc.contributor.author | 曾國榮 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:59:50Z | - |
| dc.date.copyright | 2007-08-08 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-30 | |
| dc.identifier.citation | 1. Akhouayri, O., Lafage-Proust, M.H., Rattner, A., Laroche, N., Caillot-Augusseau, A., Alexandre, C. and Vico, L. (1999) Effects of static or dynamic mechanical stresses on osteoblast phenotype expression in three-dimensional contractile collagen gels. J Cell Biochem, 76, 217-230.
2. Andrian, E., Mostefaoui, Y., Rouabhia, M. and Grenier, D. (2007) Regulation of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases by Porphyromonas gingivalis in an engineered human oral mucosa model. J Cell Physiol, 211, 56-62. 3. Angel, P., Baumann, I., Stein, B., Delius, H., Rahmsdorf, H.J. and Herrlich, P. (1987) 12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region. Mol Cell Biol, 7, 2256-2266. 4. Angel, P., Imagawa, M., Chiu, R., Stein, B., Imbra, R.J., Rahmsdorf, H.J., Jonat, C., Herrlich, P. and Karin, M. (1987) Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor. Cell, 49, 729-739. 5. Angel, P. and Karin, M. (1991) The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochim Biophys Acta, 1072, 129-157. 6. Baillat, D., Leprivier, G., Regnier, D., Vintonenko, N., Begue, A., Stehelin, D. and Aumercier, M. (2006) Stromelysin-1 expression is activated in vivo by Ets-1 through palindromic head-to-head Ets binding sites present in the promoter. Oncogene, 25, 5764-5776. 7. Banes, A.J., Gilbert, J., Taylor, D. and Monbureau, O. (1985) A new vacuum-operated stress-providing instrument that applies static or variable duration cyclic tension or compression to cells in vitro. J Cell Sci, 75, 35-42. 8. Benbow, U. and Brinckerhoff, C.E. (1997) The AP-1 site and MMP gene regulation: what is all the fuss about? Matrix Biol, 15, 519-526. 9. Bergelson, S. and Daniel, V. (1994) Cooperative interaction between Ets and AP-1 transcription factors regulates induction of glutathione S-transferase Ya gene expression. Biochem Biophys Res Commun, 200, 290-297. 10. Bond, M., Chase, A.J., Baker, A.H. and Newby, A.C. (2001) Inhibition of transcription factor NF-kappaB reduces matrix metalloproteinase-1, -3 and -9 production by vascular smooth muscle cells. Cardiovasc Res, 50, 556-565. 11. Bord, S., Horner, A., Hembry, R.M. and Compston, J.E. (1998) Stromelysin-1 (MMP-3) and stromelysin-2 (MMP-10) expression in developing human bone: potential roles in skeletal development. Bone, 23, 7-12. 12. Bord, S., Horner, A., Hembry, R.M., Reynolds, J.J. and Compston, J.E. (1996) Production of collagenase by human osteoblasts and osteoclasts in vivo. Bone, 19, 35-40. 13. Boutahar, N., Guignandon, A., Vico, L. and Lafage-Proust, M.H. (2004) Mechanical strain on osteoblasts activates autophosphorylation of focal adhesion kinase and proline-rich tyrosine kinase 2 tyrosine sites involved in ERK activation. J Biol Chem, 279, 30588-30599. 14. Chakraborti, S., Mandal, M., Das, S., Mandal, A. and Chakraborti, T. (2003) Regulation of matrix metalloproteinases: an overview. Mol Cell Biochem, 253, 269-285. 15. Chakraborti, T., Das, S., Mandal, M., Mandal, A. and Chakraborti, S. (2002) Role of Ca2+-dependent metalloprotease-2 in stimulating Ca2+ ATPase activity under peroxynitrite treatment in bovine pulmonary artery smooth muscle membrane. IUBMB Life, 53, 167-173. 16. Charles, J.M. and Key, L.L. (1998) Developmental spectrum of children with congenital osteopetrosis. J Pediatr, 132, 371-374. 17. Chaussain-Miller, C., Fioretti, F., Goldberg, M. and Menashi, S. (2006) The role of matrix metalloproteinases (MMPs) in human caries. J Dent Res, 85, 22-32. 18. Chiu, R., Angel, P. and Karin, M. (1989) Jun-B differs in its biological properties from, and is a negative regulator of, c-Jun. Cell, 59, 979-986. 19. Clark, J.M. (1988) Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res, 16, 9677-9686. 20. Couillard, J., Demers, M., Lavoie, G. and St-Pierre, Y. (2006) The role of DNA hypomethylation in the control of stromelysin gene expression. Biochem Biophys Res Commun, 342, 1233-1239. 21. Das, S., Chakraborti, T., Mandal, M., Mandal, A. and Chakraborti, S. (2002) Role of membrane-associated Ca+ dependent matrix metalloprotease-2 in the oxidant activation of Ca2+Atpase by tertiary butylhydroperoxide. Mol Cell Biochem, 237, 85-93. 22. De Croos, J.N., Dhaliwal, S.S., Grynpas, M.D., Pilliar, R.M. and Kandel, R.A. (2006) Cyclic compressive mechanical stimulation induces sequential catabolic and anabolic gene changes in chondrocytes resulting in increased extracellular matrix accumulation. Matrix Biol, 25, 323-331. 23. Dempster, D.W., Cosman, F., Parisien, M., Shen, V. and Lindsay, R. (1993) Anabolic actions of parathyroid hormone on bone. Endocr Rev, 14, 690-709. 24. Dittmer, J. and Nordheim, A. (1998) Ets transcription factors and human disease. Biochim Biophys Acta, 1377, F1-11. 25. Doyle, G.A., Pierce, R.A. and Parks, W.C. (1997) Transcriptional induction of collagenase-1 in differentiated monocyte-like (U937) cells is regulated by AP-1 and an upstream C/EBP-beta site. J Biol Chem, 272, 11840-11849. 26. Fermor, B., Weinberg, J.B., Pisetsky, D.S., Misukonis, M.A., Banes, A.J. and Guilak, F. (2001) The effects of static and intermittent compression on nitric oxide production in articular cartilage explants. J Orthop Res, 19, 729-737. 27. Gack, S., Vallon, R., Schaper, J., Ruther, U. and Angel, P. (1994) Phenotypic alterations in fos-transgenic mice correlate with changes in Fos/Jun-dependent collagenase type I expression. Regulation of mouse metalloproteinases by carcinogens, tumor promoters, cAMP, and Fos oncoprotein. J Biol Chem, 269, 10363-10369. 28. Garrington, T.P. and Johnson, G.L. (1999) Organization and regulation of mitogen-activated protein kinase signaling pathways. Curr Opin Cell Biol, 11, 211-218. 29. Goga, Y., Chiba, M., Shimizu, Y. and Mitani, H. (2006) Compressive force induces osteoblast apoptosis via caspase-8. J Dent Res, 85, 240-244. 30. Hatton, J.P., Pooran, M., Li, C.F., Luzzio, C. and Hughes-Fulford, M. (2003) A short pulse of mechanical force induces gene expression and growth in MC3T3-E1 osteoblasts via an ERK 1/2 pathway. J Bone Miner Res, 18, 58-66. 31. Heath, J.K., Reynolds, J.J. and Meikle, M.C. (1990) Osteopetrotic (grey-lethal) bone produces collagenase and TIMP in organ culture: regulation by vitamin A. Biochem Biophys Res Commun, 168, 1171-1176. 32. Hill, P.A., Murphy, G., Docherty, A.J., Hembry, R.M., Millican, T.A., Reynolds, J.J. and Meikle, M.C. (1994) The effects of selective inhibitors of matrix metalloproteinases (MMPs) on bone resorption and the identification of MMPs and TIMP-1 in isolated osteoclasts. J Cell Sci, 107 ( Pt 11), 3055-3064. 33. Hosseini, M., Rose, A.Y., Song, K., Bohan, C., Alexander, J.P., Kelley, M.J. and Acott, T.S. (2006) IL-1 and TNF induction of matrix metalloproteinase-3 by c-Jun N-terminal kinase in trabecular meshwork. Invest Ophthalmol Vis Sci, 47, 1469-1476. 34. Kanzaki, H., Chiba, M., Shimizu, Y. and Mitani, H. (2002) Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis. J Bone Miner Res, 17, 210-220. 35. Kapur, S., Baylink, D.J. and Lau, K.H. (2003) Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways. Bone, 32, 241-251. 36. Karin, M., Liu, Z. and Zandi, E. (1997) AP-1 function and regulation. Curr Opin Cell Biol, 9, 240-246. 37. Kim, S.J., Lafyatis, R., Kim, K.Y., Angel, P., Fujiki, H., Karin, M., Sporn, M.B. and Roberts, A.B. (1990) Regulation of collagenase gene expression by okadaic acid, an inhibitor of protein phosphatases. Cell Regul, 1, 269-278. 38. Kinoshita, S., Finnegan, M., Bucholz, R.W. and Mizuno, K. (1999) Three-dimensional collagen gel culture promotes osteoblastic phenotype in bone marrow derived cells. Kobe J Med Sci, 45, 201-211. 39. Lee, J.S., See, R.H., Deng, T. and Shi, Y. (1996) Adenovirus E1A downregulates cJun- and JunB-mediated transcription by targeting their coactivator p300. Mol Cell Biol, 16, 4312-4326. 40. Lee, T.H., Lee, G.W., Ziff, E.B. and Vilcek, J. (1990) Isolation and characterization of eight tumor necrosis factor-induced gene sequences from human fibroblasts. Mol Cell Biol, 10, 1982-1988. 41. Leppa, S., Saffrich, R., Ansorge, W. and Bohmann, D. (1998) Differential regulation of c-Jun by ERK and JNK during PC12 cell differentiation. Embo J, 17, 4404-4413. 42. Li, Y.C., Amling, M., Pirro, A.E., Priemel, M., Meuse, J., Baron, R., Delling, G. and Demay, M.B. (1998) Normalization of mineral ion homeostasis by dietary means prevents hyperparathyroidism, rickets, and osteomalacia, but not alopecia in vitamin D receptor-ablated mice. Endocrinology, 139, 4391-4396. 43. Liacini, A., Sylvester, J. and Zafarullah, M. (2005) Triptolide suppresses proinflammatory cytokine-induced matrix metalloproteinase and aggrecanase-1 gene expression in chondrocytes. Biochem Biophys Res Commun, 327, 320-327. 44. Mandal, M., Mandal, A., Das, S., Chakraborti, T. and Sajal, C. (2003) Clinical implications of matrix metalloproteinases. Mol Cell Biochem, 252, 305-329. 45. Maroulakou, I.G. and Bowe, D.B. (2000) Expression and function of Ets transcription factors in mammalian development: a regulatory network. Oncogene, 19, 6432-6442. 46. Masi, L., Franchi, A., Santucci, M., Danielli, D., Arganini, L., Giannone, V., Formigli, L., Benvenuti, S., Tanini, A., Beghe, F. et al. (1992) Adhesion, growth, and matrix production by osteoblasts on collagen substrata. Calcif Tissue Int, 51, 202-212. 47. Massova, I., Kotra, L.P., Fridman, R. and Mobashery, S. (1998) Matrix metalloproteinases: structures, evolution, and diversification. Faseb J, 12, 1075-1095. 48. McLeod, K.J., Rubin, C.T., Otter, M.W. and Qin, Y.X. (1998) Skeletal cell stresses and bone adaptation. Am J Med Sci, 316, 176-183. 49. Meikle, M.C., Bord, S., Hembry, R.M., Compston, J., Croucher, P.I. and Reynolds, J.J. (1992) Human osteoblasts in culture synthesize collagenase and other matrix metalloproteinases in response to osteotropic hormones and cytokines. J Cell Sci, 103 ( Pt 4), 1093-1099. 50. Minden, A. and Karin, M. (1997) Regulation and function of the JNK subgroup of MAP kinases. Biochim Biophys Acta, 1333, F85-104. 51. Mitsui, N., Suzuki, N., Koyama, Y., Yanagisawa, M., Otsuka, K., Shimizu, N. and Maeno, M. (2006) Effect of compressive force on the expression of MMPs, PAs, and their inhibitors in osteoblastic Saos-2 cells. Life Sci, 79, 575-583. 52. Nuki, K., Soskolne, W.A., Raisz, L.G., Kornman, K.S. and Alander, C. (1981) Bone resorbing activity of gingiva from beagle dogs following metronidazole and indomethacin therapy. J Periodontal Res, 16, 205-212. 53. O'Hagan, R.C., Tozer, R.G., Symons, M., McCormick, F. and Hassell, J.A. (1996) The activity of the Ets transcription factor PEA3 is regulated by two distinct MAPK cascades. Oncogene, 13, 1323-1333. 54. Oikawa, T. and Yamada, T. (2003) Molecular biology of the Ets family of transcription factors. Gene, 303, 11-34. 55. Pacifici, R. (1998) Cytokines, estrogen, and postmenopausal osteoporosis--the second decade. Endocrinology, 139, 2659-2661. 56. Prestwood, K.M., Pilbeam, C.C. and Raisz, L.G. (1995) Treatment of osteoporosis. Annu Rev Med, 46, 249-256. 57. Raisz, L.G. (1988) Hormonal regulation of bone growth and remodelling. Ciba Found Symp, 136, 226-238. 58. Raisz, L.G. (1997) The osteoporosis revolution. Ann Intern Med, 126, 458-462. 59. Raisz, L.G. (1999) Physiology and pathophysiology of bone remodeling. Clin Chem, 45, 1353-1358. 60. Ravanti, L. and Kahari, V.M. (2000) Matrix metalloproteinases in wound repair (review). Int J Mol Med, 6, 391-407. 61. Reunanen, N., Li, S.P., Ahonen, M., Foschi, M., Han, J. and Kahari, V.M. (2002) Activation of p38 alpha MAPK enhances collagenase-1 (matrix metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) expression by mRNA stabilization. J Biol Chem, 277, 32360-32368. 62. Ribbens, C., Andre, B., Kaye, O., Kaiser, M.J., Bonnet, V., Jaspar, J.M., de Groote, D., Franchimont, N. and Malaise, M.G. (2000) Synovial fluid matrix metalloproteinase-3 levels are increased in inflammatory arthritides whether erosive or not. Rheumatology (Oxford), 39, 1357-1365. 63. Robinson, M.J. and Cobb, M.H. (1997) Mitogen-activated protein kinase pathways. Curr Opin Cell Biol, 9, 180-186. 64. Rodan, S.B., Rodan, G.A., Simmons, H.A., Walenga, R.W., Feinstein, M.B. and Raisz, L.G. (1981) Bone resorptive factor produced by osteosarcoma cells with osteoblastic features is PGE2. Biochem Biophys Res Commun, 102, 1358-1365. 65. Saus, J., Quinones, S., Otani, Y., Nagase, H., Harris, E.D., Jr. and Kurkinen, M. (1988) The complete primary structure of human matrix metalloproteinase-3. Identity with stromelysin. J Biol Chem, 263, 6742-6745. 66. Sharrocks, A.D., Brown, A.L., Ling, Y. and Yates, P.R. (1997) The ETS-domain transcription factor family. Int J Biochem Cell Biol, 29, 1371-1387. 67. Silence, J., Lupu, F., Collen, D. and Lijnen, H.R. (2001) Persistence of atherosclerotic plaque but reduced aneurysm formation in mice with stromelysin-1 (MMP-3) gene inactivation. Arterioscler Thromb Vasc Biol, 21, 1440-1445. 68. Sirum, K.L. and Brinckerhoff, C.E. (1989) Cloning of the genes for human stromelysin and stromelysin 2: differential expression in rheumatoid synovial fibroblasts. Biochemistry, 28, 8691-8698. 69. Soskolne, W.A., Nuki, K., Raisz, L.G. and Alander, C. (1980) The appearance of bone resorbing activity in ligature induced gingivitis in dogs. J Periodontal Res, 15, 373-379. 70. Tasevski, V., Sorbetti, J.M., Chiu, S.S., Shrive, N.G. and Hart, D.A. (2005) Influence of mechanical and biological signals on gene expression in human MG-63 cells: evidence for a complex interplay between hydrostatic compression and vitamin D3 or TGF-beta1 on MMP-1 and MMP-3 mRNA levels. Biochem Cell Biol, 83, 96-107. 71. Thomson, B.M., Atkinson, S.J., McGarrity, A.M., Hembry, R.M., Reynolds, J.J. and Meikle, M.C. (1989) Type I collagen degradation by mouse calvarial osteoblasts stimulated with 1,25-dihydroxyvitamin D-3: evidence for a plasminogen-plasmin-metalloproteinase activation cascade. Biochim Biophys Acta, 1014, 125-132. 72. Tiilikainen, P., Pirttiniemi, P., Kainulainen, T., Pernu, H. and Raustia, A. (2005) MMP-3 and -8 expression is found in the condylar surface of temporomandibular joints with internal derangement. J Oral Pathol Med, 34, 39-45. 73. Topcu, Z. (2000) An optimized recipe for cloning of the polymerase chain reaction-amplified DNA inserts into plasmid vectors. Acta Biochim Pol, 47, 841-846. 74. Trojanowska, M. (2000) Ets factors and regulation of the extracellular matrix. Oncogene, 19, 6464-6471. 75. Twining, S.S. (1994) Regulation of proteolytic activity in tissues. Crit Rev Biochem Mol Biol, 29, 315-383. 76. Ungefroren, H., Groth, S., Ruhnke, M., Kalthoff, H. and Fandrich, F. (2005) Transforming growth factor-beta (TGF-beta) type I receptor/ALK5-dependent activation of the GADD45beta gene mediates the induction of biglycan expression by TGF-beta. J Biol Chem, 280, 2644-2652. 77. Van Coillie, E., Van Aelst, I., Fiten, P., Billiau, A., Van Damme, J. and Opdenakker, G. (1999) Transcriptional control of the human MCP-2 gene promoter by IFN-gamma and IL-1beta in connective tissue cells. J Leukoc Biol, 66, 502-511. 78. Vincenti, M.P., White, L.A., Schroen, D.J., Benbow, U. and Brinckerhoff, C.E. (1996) Regulating expression of the gene for matrix metalloproteinase-1 (collagenase): mechanisms that control enzyme activity, transcription, and mRNA stability. Crit Rev Eukaryot Gene Expr, 6, 391-411. 79. Wadhwa, S., Godwin, S.L., Peterson, D.R., Epstein, M.A., Raisz, L.G. and Pilbeam, C.C. (2002) Fluid flow induction of cyclo-oxygenase 2 gene expression in osteoblasts is dependent on an extracellular signal-regulated kinase signaling pathway. J Bone Miner Res, 17, 266-274. 80. Webb, S.J., McPherson, J.R., Pahan, K. and Koka, S. (2002) Regulation of TNF-alpha-induced IL-6 production in MG-63 human osteoblast-like cells. J Dent Res, 81, 17-22. 81. Westermarck, J., Holmstrom, T., Ahonen, M., Eriksson, J.E. and Kahari, V.M. (1998) Enhancement of fibroblast collagenase-1 (MMP-1) gene expression by tumor promoter okadaic acid is mediated by stress-activated protein kinases Jun N-terminal kinase and p38. Matrix Biol, 17, 547-557. 82. Westermarck, J. and Kahari, V.M. (1999) Regulation of matrix metalloproteinase expression in tumor invasion. Faseb J, 13, 781-792. 83. Westermarck, J., Lohi, J., Keski-Oja, J. and Kahari, V.M. (1994) Okadaic acid-elicited transcriptional activation of collagenase gene expression in HT-1080 fibrosarcoma cells is mediated by JunB. Cell Growth Differ, 5, 1205-1213. 84. Westermarck, J., Seth, A. and Kahari, V.M. (1997) Differential regulation of interstitial collagenase (MMP-1) gene expression by ETS transcription factors. Oncogene, 14, 2651-2660. 85. Woessner, J.F., Jr. (1991) Matrix metalloproteinases and their inhibitors in connective tissue remodeling. Faseb J, 5, 2145-2154. 86. Yabe, H., Fukuma, M., Urano, F., Yoshida, K., Kato, S., Toyama, Y., Hata, J. and Umezawa, A. (2002) Lack of matrix metalloproteinase (MMP)-1 and -3 expression in Ewing sarcoma may be due to loss of accessibility of the MMP regulatory element to the specific fusion protein in vivo. Biochem Biophys Res Commun, 293, 61-71. 87. Yang, C.M., Chien, C.S., Yao, C.C., Hsiao, L.D., Huang, Y.C. and Wu, C.B. (2004) Mechanical strain induces collagenase-3 (MMP-13) expression in MC3T3-E1 osteoblastic cells. J Biol Chem, 279, 22158-22165. 88. Yoshikawa, H. and Takaoka, K. (1994) [Bone remodelling and its regulators]. Nippon Rinsho, 52, 2262-2267. 89. You, J., Reilly, G.C., Zhen, X., Yellowley, C.E., Chen, Q., Donahue, H.J. and Jacobs, C.R. (2001) Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium mobilization and activation of mitogen-activated protein kinase in MC3T3-E1 osteoblasts. J Biol Chem, 276, 13365-13371. 90. 翁竹音：週期性壓力刺激對類成骨母細胞基因表現的影響。國立台灣大學醫學院臨床牙醫學研究所碩士論文。2005。 91. 汪文琲：細胞間質代謝酶在矯正治療中的角色。國立台灣大學醫學院臨床牙醫學研究所碩士論文。2006。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24995 | - |
| dc.description.abstract | 機械力量的刺激對骨骼在生長發育中的重塑扮演重要的角色，但力量刺激對細胞內基因變化所受到的調控，目前仍然不清楚。之前的研究已利用基因微陣列分析，篩選出43 個基因在MG-63 類成骨細胞受壓力刺激後有差異性的表現，其中細胞外間質代謝酶三(Matrix Metalloproteinase 3, MMP-3)受壓力刺激後在RNA層次（上升2.3 倍）及Protein 層次上會有上升的情形。本實驗先從人類細胞設計
兩段帶有酵素切位的MMP-3 promoter primer，利用聚合酶連鎖反應（PCR）夾出約3.2K bp 的片段後，接到pGL3-basic vector 及pEGFP-1 vector 上，純化出轉殖成功的pGL3 basic-MMP-3 及pEGFP-1-MMP-3 plasmid。將上述plasmid 轉染到MG-63 類成骨細胞內，之後進行張力及壓力實驗。當帶有pGL3 basic-MMP-3 及pEGFP-1-MMP-3 plasmid 的細胞受到張力刺激，分別利用冷光分析儀讀取冷光數 值及倒立式螢光顯微鏡直接觀察後，發現MMP-3 promoter 活性調控受到張力刺激會有微量下降的情形。在西方墨點法分析中亦可見細胞內MMP-3 protein 受張力刺激後，有微量的下降。而在受到壓力的刺激後，利用冷光分析儀讀取冷光數值及西方墨點法分析細胞內MMP-3 protein 表現量，發現會有明顯的上升。因此，週期性張力及壓力刺激在對MMP-3 promoter 基因調控，蛋白質的表現量反應不同，前者為正向調控，後者為負向調控。 | zh_TW |
| dc.description.abstract | It is widely accepted that mechanical loading is necessary to construct the architecture of bone and to maintain bone mass. Mechanical strain plays an important role in bone remodeling during growth and development. However, the cellular gene expression that controls the tissue response to mechanical force remains elusive. Previous experiments detected increased expression of matrix metalloproteinase-3 (mmp-3) when cyclic compression force was applied to MG-63 osteoblast-like cell in 3D collagen gel. Here, I cloned a 3.2 K-mmp-3 promoter fragment from human normal and HeLa cell by polymerase chain reaction and inserted this DNA into pGL3-Basic and pEGFP-1 vectors. Purified promoter-reporter constructs were transfected into MG-63 cells and cells were then processed through cyclic tension and compression stimulation for one day. The results indicate that the MMP-3 promoter activity was slightly down regulated by cyclic tension stimulation. However, promoter activity was up regulated by cyclic compression. Hence, mmp-3 promoter activity could be differentially regulated when osteoblasts experience different patterns of mechanical stimulation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:59:50Z (GMT). No. of bitstreams: 1 ntu-96-R94450009-1.pdf: 2862697 bytes, checksum: 4c386f1255fe3683e1f17b4a3f1e60e9 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目錄………………………………………………………………………….. I
表次目錄………………………………………………………………… …. II 圖次目錄……………………………………………………………………..III 中文摘要……………………………………………………………………VII 英文摘要…………………………………………………………………...VIII 第一章 引言…………………………………………………………................1 第二章 實驗目的……………………………………………………………20 第三章 實驗材料與方法………………………………………………….…21 第四章 實驗結果………………………………………………………...…..36 第五章 討論……………………………………………………………...…..42 第六章 結論…………………………………………………………………52 第七章 未來研究方向……………………………………………………….54 第八章 參考文獻……………………………………………………………80 | |
| dc.language.iso | zh-TW | |
| dc.subject | 週期性壓力 | zh_TW |
| dc.subject | 細胞外基質金屬蛋白 | zh_TW |
| dc.subject | 週期性張力 | zh_TW |
| dc.subject | MMP-3 promoter | en |
| dc.subject | Cyclic Tension | en |
| dc.subject | MG-63 cells | en |
| dc.subject | Cyclic Compression | en |
| dc.title | 週期性張力與壓力刺激對細胞外基質金屬蛋白酶-3基因啟動子的調控 | zh_TW |
| dc.title | Regulation of Extracellular Matrix Metalloproteinase-3
（MMP-3）Promoter in MG-63 cells under Cyclic Tension and Compression Force Stimulation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳羿貞(Yi-Jane Chen),郭彥彬(Yen-Ping Kuo),張百恩(Bei-En Chang) | |
| dc.subject.keyword | 週期性張力,週期性壓力,細胞外基質金屬蛋白, | zh_TW |
| dc.subject.keyword | Cyclic Tension,Cyclic Compression,MMP-3 promoter,MG-63 cells, | en |
| dc.relation.page | 92 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2007-07-31 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 2.8 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。