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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王愛玉(Ai-Yu Wang) | |
| dc.contributor.author | Ching-Lin Tsai | en |
| dc.contributor.author | 蔡青霖 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:57:57Z | - |
| dc.date.copyright | 2007-09-03 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-08-29 | |
| dc.identifier.citation | Albrecht G, Mustroph A (2003) Localization of sucrose synthase in wheat roots: increased in situ activity of sucrose synthase correlates with cell wall thickening by cellulose deposition under hypoxia. Planta 217: 252-260
Amor Y, Haigler CH, Johnson S, Wainscott M, Delmer DP (1995) A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc Natl Acad Sci U S A 92: 9353-9357 Anguenot R, Yelle S, Nguyen-Quoc B (1999) Purification of tomato sucrose synthase phosphorylated isoforms by Fe(III)-immobilized metal affinity chromatography. Arch Biochem Biophys 365: 163-169 Asano T, Kunieda N, Omura Y, Ibe H, Kawasaki T, Takano M, Sato M, Furuhashi H, Mujin T, Takaiwa F, Wu Cy CY, Tada Y, Satozawa T, Sakamoto M, Shimada H (2002) Rice SPK, a calmodulin-like domain protein kinase, is required for storage product accumulation during seed development: phosphorylation of sucrose synthase is a possible factor. Plant Cell 14: 619-628 Baroja-Fernandez E, Munoz FJ, Zandueta-Criado A, Moran-Zorzano MT, Viale AM, Alonso-Casajus N, Pozueta-Romero J (2004) Most of ADP x glucose linked to starch biosynthesis occurs outside the chloroplast in source leaves. Proc Natl Acad Sci U S A 101: 13080-13085 Barratt DH, Barber L, Kruger NJ, Smith AM, Wang TL, Martin C (2001) Multiple, distinct isoforms of sucrose synthase in pea. Plant Physiol 127: 655-664 Bieniawska Z, Paul Barratt DH, Garlick AP, Thole V, Kruger NJ, Martin C, Zrenner R, Smith AM (2007) Analysis of the sucrose synthase gene family in Arabidopsis. Plant J 49: 810-828 Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513-1523 Bologa KL, Fernie AR, Leisse A, Loureiro ME, Geigenberger P (2003) A bypass of sucrose synthase leads to low internal oxygen and impaired metabolic performance in growing potato tubers. Plant Physiol 132: 2058-2072 Bordier C (1981) Phase separation of integral membrane proteins in Triton X-114 solution. J Biol Chem 256: 1604-1607 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254 Cardini CE, Leloir LF, Chiriboga J (1955) The biosynthesis of sucrose. J Biol Chem 214: 149-155 Carlson SJ, Chourey PS, Helentjaris T, Datta R (2002) Gene expression studies on developing kernels of maize sucrose synthase (SuSy) mutants show evidence for a third SuSy gene. Plant Mol Biol 49: 15-29 Chan HY, Ling TY, Juang RH, Ting IN, Sung HY, Su JC (1990) Sucrose Synthase in Rice Plants : Growth-Associated Changes in Tissue Specific Distributions. Plant Physiol 94: 1456-1461 Chiu WB, Lin CH, Chang CJ, Hsieh MH, Wang AY (2006) Molecular characterization and expression of four cDNAs encoding sucrose synthase from green bamboo Bambusa oldhamii. New Phytol 170: 53-63 Chourey PS (1981) Genetic control of sucrose synthase in maize endosperm. Molecular and general genetics 184: 372-376 Dejardin A, Sokolov LN, Kleczkowski LA (1999) Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis. Biochem J 344 Pt 2: 503-509 Duncan KA, Hardin SC, Huber SC (2006) The three maize sucrose synthase isoforms differ in distribution, localization, and phosphorylation. Plant Cell Physiol 47: 959-971 Echt CS, Chourey PS (1985) A Comparison of Two Sucrose Synthetase Isozymes from Normal and shrunken-1 Maize. Plant Physiol 79: 530-536 Elling L (1995) Effect of metal ions on sucrose synthase from rice grains--a study on enzyme inhibition and enzyme topography. Glycobiology 5: 201-206 Elling L, Grothus M, Kula MR (1993) Investigation of sucrose synthase from rice for the synthesis of various nucleotide sugars and saccharides. Glycobiology 3: 349-355 Etxeberria E, Gonzalez P (2003) Evidence for a tonoplast-associated form of sucrose synthase and its potential involvement in sucrose mobilization from the vacuole. J Exp Bot 54: 1407-1414 Fu H, Kim SY, Park WD (1995) High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 5' and 3' flanking sequences and the leader intron. Plant Cell 7: 1387-1394 Fu H, Kim SY, Park WD (1995) A potato Sus3 sucrose synthase gene contains a context-dependent 3' element and a leader intron with both positive and negative tissue-specific effects. Plant Cell 7: 1395-1403 Geigenberger P (2003) Regulation of sucrose to starch conversion in growing potato tubers. J Exp Bot 54: 457-465 Hanahan D (1985) Techniques for transformation of E. coli. In DM Glover, BD Hames, eds, DNA Cloning: A Practical Approach, Vol 1 : Core Techniques. IRL Press, pp 103-109 Hanggi E, Fleming AJ (2001) Sucrose synthase expression pattern in young maize leaves: implications for phloem transport. Planta 214: 326-329 Hardin SC, Tang GQ, Scholz A, Holtgraewe D, Winter H, Huber SC (2003) Phosphorylation of sucrose synthase at serine 170: occurrence and possible role as a signal for proteolysis. Plant J 35: 588-603 Hardin SC, Winter H, Huber SC (2004) Phosphorylation of the amino terminus of maize sucrose synthase in relation to membrane association and enzyme activity. Plant Physiol 134: 1427-1438 Horst I, Welham T, Kelly S, Kaneko T, Sato S, Tabata S, Parniske M, Wang TL (2007) TILLING mutants of Lotus japonicus reveal that nitrogen assimilation and fixation can occur in the absence of nodule-enhanced sucrose synthase. Plant Physiol 144: 806-820 Huang DY, Wang AY (1998) Purification and characterization of sucrose synthase isozymes from etiolated rice seedlings. Biochem Mol Biol Int 46: 107-113 Huang JW, Chen JT, Yu WP, Shyur LF, Wang AY, Sung HY, Lee PD, Su JC (1996) Complete structures of three rice sucrose synthase isogenes and differential regulation of their expressions. Biosci Biotechnol Biochem 60: 233-239 Huang YH, Picha DH, Kilili AW (1999) A continuous method for enzymatic assay of sucrose synthase in the synthetic direction. J Agric Food Chem 47: 2746-2750 Hubbard NL, Huber SC, Pharr DM (1989) Sucrose Phosphate Synthase and Acid Invertase as Determinants of Sucrose Concentration in Developing Muskmelon (Cucumis melo L.) Fruits. Plant Physiol 91: 1527-1534 Huber SC, Huber JL, Liao PC, Gage DA, McMichael RW, Jr., Chourey PS, Hannah LC, Koch K (1996) Phosphorylation of serine-15 of maize leaf sucrose synthase. Occurrence in vivo and possible regulatory significance. Plant Physiol 112: 793-802 Klotz KL, Haagenson DM (2007) Wounding, anoxia and cold induce sugarbeet sucrose synthase transcriptional changes that are unrelated to protein expression and activity. J Plant Physiol Koch K (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7: 235-246 Koch KE, Nolte KD, Duke ER, McCarty DR, Avigne WT (1992) Sugar Levels Modulate Differential Expression of Maize Sucrose Synthase Genes. Plant Cell 4: 59-69 Konishi T, Ohmiya Y, Hayashi T (2004) Evidence that sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem. Plant Physiol 134: 1146-1152 Kutschera U, Heiderich A (2002) Sucrose metabolism and cellulose biosynthesis in sunflower hypocotyls. Physiol Plant 114: 372-379 Larsen AE, Salerno GL, Pontis HG (1985) Sucrose synthase from wheat leaves. Comparison with the wheat germ enzyme Physiologia Plantarum 67: 37-42 Liao YC, Wang AY (2003) Sugar-modulated gene expression of sucrose synthase in suspension-cultured cells of rice. Physiologia Plantarum 118: 319-327 Martin T, Frommer WB, Salanoubat M, Willmitzer L (1993) Expression of an Arabidopsis sucrose synthase gene indicates a role in metabolization of sucrose both during phloem loading and in sink organs. Plant J 4: 367-377 Matic S, Akerlund HE, Everitt E, Widell S (2004) Sucrose synthase isoforms in cultured tobacco cells. Plant Physiol Biochem 42: 299-306 McCarty DR, Shaw JR, Hannah LC (1986) The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci U S A 83: 9099-9103 Nakai T, Konishi T, Zhang XQ, Chollet R, Tonouchi N, Tsuchida T, Yoshinaga F, Mori H, Sakai F, Hayashi T (1998) An increase in apparent affinity for sucrose of mung bean sucrose synthase is caused by in vitro phosphorylation or directed mutagenesis of Ser11. Plant Cell Physiol 39: 1337-1341 Nakai T, Tonouchi N, Konishi T, Kojima Y, Tsuchida T, Yoshinaga F, Sakai F, Hayashi T (1999) Enhancement of cellulose production by expression of sucrose synthase in acetobacter xylinum. Proc Natl Acad Sci U S A 96: 14-18 Nolte KD, Hendrix DL, Radin JW, Koch KE (1995) Sucrose Synthase Localization during Initiation of Seed Development and Trichome Differentiation in Cotton Ovules. Plant Physiol 109: 1285-1293 Nolte KD, Koch KE (1993) Companion-Cell Specific Localization of Sucrose Synthase in Zones of Phloem Loading and Unloading. Plant Physiol 101: 899-905 Pozueta-Romero J, Yamaguchi J, Akazawa T (1991) ADPG formation by the ADP-specific cleavage of sucrose-reassessment of sucrose synthase. FEBS Lett 291: 233-237 Romer U, Schrader H, Gunther N, Nettelstroth N, Frommer WB, Elling L (2004) Expression, purification and characterization of recombinant sucrose synthase 1 from Solanum tuberosum L. for carbohydrate engineering. J Biotechnol 107: 135-149 Rowland LJ, Chen YC, Chourey PS (1989) Anaerobic treatment alters the cell specific expression of Adh-1, Sh, and Sus genes in roots of maize seedlings. Molecular and General Genetics 218: 1432-1874 Ruan YL, Llewellyn DJ, Furbank RT (2003) Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell 15: 952-964 Salnikov VV, Grimson MJ, Seagull RW, Haigler CH (2003) Localization of sucrose synthase and callose in freeze-substituted secondary-wall-stage cotton fibers. Protoplasma 221: 175-184 Shaw JR, Ferl RJ, Baier J, St Clair D, Carson C, McCarty DR, Hannah LC (1994) Structural features of the maize sus1 gene and protein. Plant Physiol 106: 1659-1665 Stobrawa K, Lorenc-Plucinska G (2007) Changes in carbohydrate metabolism in fine roots of the native European black poplar (Populus nigra L.) in a heavy-metal-polluted environment. Sci Total Environ 373: 157-165 Su JC, Wu JL, L. LYC (1977) Purification and Characterization of Sucrose Synthetase from the Shoot of Bamboo Leleba oldhami. plant physiology 60: 17-21 Subbaiah CC, Palaniappan A, Duncan K, Rhoads DM, Huber SC, Sachs MM (2006) Mitochondrial localization and putative signaling function of sucrose synthase in maize. J Biol Chem 281: 15625-15635 Subbaiah CC, Sachs MM (2001) Altered patterns of sucrose synthase phosphorylation and localization precede callose induction and root tip death in anoxic maize seedlings. Plant Physiol 125: 585-594 Sung HY, Su JC (1977) Sucrose synthase isozymes of pea seedling -- purification and general properties. Journal Chinese Biochemistry Society 6: 22-37 Tsai ZC, Wang AY (2003) Identification of rice manganese-dependent protein kinases that phosphorylate sucrose synthase at multiple serine residues. Botanical bulletin of Academia Sinica 44: 141-150 Uggla C, Magel E, Moritz T, Sundberg B (2001) Function and dynamics of auxin and carbohydrates during earlywood/latewood transition in scots pine. Plant Physiol 125: 2029-2039 Wachter R, Langhans M, Aloni R, Gotz S, Weilmunster A, Koops A, Temguia L, Mistrik I, Pavlovkin J, Rascher U, Schwalm K, Koch KE, Ullrich CI (2003) Vascularization, high-volume solution flow, and localized roles for enzymes of sucrose metabolism during tumorigenesis by Agrobacterium tumefaciens. Plant Physiol 133: 1024-1037 Wang AY, Kao MH, Yang WH, Sayion Y, Liu LF, Lee PD, Su JC (1999) Differentially and developmentally regulated expression of three rice sucrose synthase genes. Plant Cell Physiol 40: 800-807 Wang AY, Yu WP, Juang RH, Huang JW, Sung HY, Su JC (1992) Presence of three rice sucrose synthase genes as revealed by cloning and sequencing of cDNA. Plant Mol Biol 18: 1191-1194 Winter H, Huber JL, Huber SC (1997) Membrane association of sucrose synthase: changes during the graviresponse and possible control by protein phosphorylation. FEBS Lett 420: 151-155 Winter H, Huber JL, Huber SC (1998) Identification of sucrose synthase as an actin-binding protein. FEBS Lett 430: 205-208 Wolosiuk RW, Pontis HG (1971) Evidence of the existence of two forms of sucrose synthetase. FEBS Lett 16: 237-240 Yu WP, Wang AY, Juang RH, Sung HY, Su JC (1992) Isolation and sequences of rice sucrose synthase cDNA and genomic DNA. Plant Mol Biol 18: 139-142 Zeng Y, Wu Y, Avigne WT, Koch KE (1998) Differential regulation of sugar-sensitive sucrose synthases by hypoxia and anoxia indicate complementary transcriptional and posttranscriptional responses. Plant Physiol 116: 1573-1583 Zeng Y, Wu Y, Avigne WT, Koch KE (1999) Rapid repression of maize invertases by low oxygen. Invertase/sucrose synthase balance, sugar signaling potential, and seedling survival. Plant Physiol 121: 599-608 Zhang XQ, Lund AA, Sarath G, Cerny RL, Roberts DM, Chollet R (1999) Soybean nodule sucrose synthase (nodulin-100): further analysis of its phosphorylation using recombinant and authentic root-nodule enzymes. Arch Biochem Biophys 371: 70-82 王愛玉 (1989) 水稻蔗糖合成酶之分子生物學研究. 博士論文. 國立臺灣大學農業化學研究所 張睿哲 (2004) 水稻蔗糖合成酶 RSus1 基因調控區域中 cis-element 之研究. 碩士論文. 國立臺灣大學微生物與生化學研究所 莊榮輝 (1985) 水稻蔗糖合成酶之研究 － 其純化、生物化學及免疫性質之比較. 博士論文. 國立臺灣大學農業化學研究所 陳姿利 (2005) 水稻蔗糖合成酶 RSuS2 在酵母菌 Pichia pastoris 中表現及生化性質檢定. 碩士論文. 國立臺灣大學微生物與生化學研究所 黃玉嬌 (2006) 水稻蔗糖合成酶 RSuS1 野生型與突變型蛋白質之表現與檢定. 碩士論文. 國立臺灣大學微生物與生化學研究所 黃如瑋 (1994) 水稻蔗糖合成酶異構基因之選殖及影響表現生理條件的研究. 博士論文. 國立臺灣大學農業化學研究所 黃卓萱 (2007) 水稻蔗糖合成酶 RSuS3 突變株之分析與結構性質探討. 碩士論文. 國立臺灣大學微生物與生化學研究所 黃德宜 (2003) 水稻蔗糖合成酶 RSuS3 基因表現與酵素功能之探討. 博士論文. 國立台灣大學農業化學研究所 蔡承佳 (2003) 蛋白質磷酸化對水稻蔗糖合成酶酵素功能及基因表現的影響. 博士論文. 國立臺灣大學農業化學研究所 蔡逸君 (2006) 酵母菌 Pichia pastoris 中表現重組水稻蔗糖合成酶 RSuS3 之性質與結構探討. 碩士論文. 國立臺灣大學微生物與生化學研究所 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24893 | - |
| dc.description.abstract | 蔗糖合成酶催化蔗糖及 UDP 轉換為果糖及 UDPG 的可逆反應。在目前已知的六種水稻蔗糖合成酶異構基因 (RSus) 中，RSus1 及 RSus3 已被轉殖至酵母菌 Pichia pastoris 並表現重組 RSuS 蛋白質。然而核苷酸定序結果顯示位於 P. pastoris 表現質體中的 RSus1 及 RSus3 cDNA 皆帶有突變。本研究建構帶有正確 RSus1 及 RSus3 ORF 序列之表現質體，並成功以 P. pastoris 表現重組野生型 RSuS1 及 RSuS3 蛋白質，同時兩重組蛋白質皆以 DEAE-Sephacel 離子交換層析、 Sephacryl S-300 膠體過濾層析及 Resource-Q 陰離子交換快速液相層析進行純化。
重組野生型 RSuS3 蛋白質的蔗糖分解與合成方向比活性皆比突變型 RSuS3 蛋白質 (mRSuS3) 高出許多，同時兩者間蔗糖分解與合成方向比活性之比值也有明顯差異。由酵素動力學的分析結果，比較對 UDPG 的親和力，重組野生型 RSuS3遠高於重組 mRSuS3。這些分析結果顯示位於 mRSuS3 上的變異，包括 T4P、V39A、D129G 及 F680S，在 RSuS3 與 UDPG 的結合上，以及 RSuS3 在蔗糖合成及分解方向的活性影響上，皆扮演著重要的角色。 由酵素動力學的研究結果亦發現，重組 RSuS1 對 UDPG 及果糖的親和力遠高於 mRSuS1；在蔗糖合成方向比活性的比較上，重組 RSuS1 亦高於 mRSuS1。重組 RSuS1 與 mRSuS1 之 C 端序列的差異可能為影響活性及與基質結合的重要原因。 | zh_TW |
| dc.description.abstract | Sucrose synthase (SuS) catalyzes the reversible conversion of sucrose and UDP into fructose and UDP-glucose. Among six known rice Sus genes (RSus), RSus1 and RSus3 were previously transformed into yeast Pichia pastoris and the recombinant RSuS proteins were expressed successfully. However, the results of nucleotide sequencing of expression plasmids containing RSuS1 or RSuS3 cDNA showed that there were mutations in both RSus1 and RSus3. In this research, the expression plasmids carrying the wild-type open reading frame of RSus1 and RSus3 were constructed and transformed into P. pastoris for expression. Both recombinant proteins were purified by DEAE-Sephacel ion-exchange chromatography, Sephacryl S-300 gel filtration chromatography and Resource-Q FPLC system.
The specific activity of recombinant RSuS3 in both sucrose cleavage and synthesis direction were much higher than those of mutated RSuS3 (mRSuS3), and the ratio of sucrose-cleavage to sucrose-synthesis specific activity was also different from mRSuS3. Moreover, the binding affinity for UDPG of RSuS3 was much higher than that of mRSuS3, as revealed by the kinetic analysis data. These results suggested that the mutations, T4P, V39A, D129G and F680S, in mRSuS3 may affect the substrate binding and also the catalytic activity in both sucrose cleavage and synthesis directions. The kinetic analysis data also showed that the binding affinity of both UDPG and fructose of RSuS1 are much higher than those of mRSuS1. The specific activity of recombinant RSuS1 in sucrose synthesis direction is much higher than that of mRSuS1. The results indicated that the mutations in the C terminus of mRSuS1 may affect the binding of UDPG and fructose to the enzyme and its activity. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:57:57Z (GMT). No. of bitstreams: 1 ntu-96-R94b47211-1.pdf: 2356733 bytes, checksum: e375be3bdf574d1bd77661322913ad76 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目錄 I
縮寫表 IV 摘要 VI Abstract VII 第一章 研究背景 1 第一節 蔗糖合成酶之生化性質 1 第二節 蔗糖合成酶之生理角色 1 2.1 參與細胞壁多醣類的合成 2 2.2 參與澱粉生合成 2 2.3 參與蔗糖運輸 3 第三節 蔗糖合成酶異構酶 3 第四節 蔗糖合成酶的磷酸化修飾 4 第五節 蔗糖合成酶活性調控因子 5 第六節 蔗糖合成酶基因的調控 5 第七節 水稻蔗糖合成酶的研究 6 第八節 本論文之研究方向及目的 8 第二章 材料與方法 9 第一節 實驗材料與藥品 9 1.1 菌種 9 1.2 質體 9 1.3 藥品 9 第二節 實驗儀器設備 10 2.1 核酸電泳設備 10 2.2 蛋白質電泳與轉印設備 10 2.3 離心機 10 2.4 其他 10 第三節 實驗方法 11 3.1 重組 RSuS 表現質體之建構 11 3.2 質體對大腸桿菌之轉形與轉形株檢定 14 3.3 質體對酵母菌 Pichia pastoris 之轉形與轉形株篩選 15 3.4 重組 RSuS 蛋白質之大量表現 18 3.5 重組 RSuS 蛋白質之純化 18 3.6 重組 RSuS 分析 20 第三章 結果與討論 29 第一節 以酵母菌 Pichia pastoris 表現重組 RSuS1 及 RSuS3 29 1.1 宿主及表現載體 29 1.2 RSuS1 及 RSuS3 表現質體之序列差異 29 1.3 表現載體 pPICZA-RSuS1 及 pPICZA-RSuS3 之建構 30 1.4 P. pastoris 轉形株之鑑定及篩選 30 1.5 P. pastoris 轉形株最適表現條件探討 31 1.6 重組 RSuS1 及 RSuS3 蛋白質之大量表現及純化 32 第二節 重組 RSuS1 及 RSuS3 蛋白質之蔗糖分解與合成方向活性比較 33 第三節 中間代謝物對重組 RSuS1 活性之影響 34 第四節 重組 RSuS1 蛋白質進行 Triton X-114 分層 35 第五節 重組 RSuS1 及 RSuS3 之酵素動力學分析 35 第六節 討論 36 第四章 結論與未來展望 39 第一節 結論 39 第二節 未來展望 39 2.1 野生型 RSuS1 及 RSuS3 生化性質及酵素動力學分析 39 2.2 野生型重組 RSuS1 及 RSuS3 之膜結合性質分析 39 2.3 重組 RSuS3 之 T4P、V39A、D129G 及 F680S 單點突變株之活性分析 40 2.4 RSuS 之結構解析 40 參考文獻 41 圖與表.............................................................................................................................................49 | |
| dc.language.iso | zh-TW | |
| dc.subject | 水稻蔗糖合成酶 | zh_TW |
| dc.subject | RSuS | en |
| dc.title | 酵母菌 Pichia pastoris 中表現之重組水稻蔗糖合成酶 RSuS1 及 RSuS3 之性質探討 | zh_TW |
| dc.title | Characterization of Recombinant Rice Sucrose Synthase RSuS1 and RSuS3 Expressed in Pichia pastoris | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 宋賢一(Hsien-Yi Sung),楊健志(Chien-Chih Yang),張珍田(Chen-Tien Chan) | |
| dc.subject.keyword | 水稻蔗糖合成酶, | zh_TW |
| dc.subject.keyword | RSuS, | en |
| dc.relation.page | 78 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2007-08-30 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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