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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃健雄(Jan-Hsiung Huang) | |
| dc.contributor.author | Pei-Ju Lee | en |
| dc.contributor.author | 李培儒 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:57:25Z | - |
| dc.date.copyright | 2007-11-15 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-11-02 | |
| dc.identifier.citation | 侯廷鉞。2006。利用Pichia pastoris 生產Candida rugosa 脂肪酶第三型。國立台灣大學微生物與生化學研究所碩士論文。
胡紹陽。2004。吳郭魚類胰島素生長因子第二型在大腸桿菌及飼料酵母菌之表現與生產條件探討。國立台灣大學微生物與生化學研究所博士論文。 陳長志。2005。大腸桿菌植酸酶於重組酵母菌Pichia pastoris 中大量表現之硏究。國立台灣大學微生物與生化學研究所博士論文。 陳佳良。2005。混合辦公室廢紙脫墨性質之研究。國立屏東科技大學木材工業系碩士學位論文。 郭亭君。2007。Pichia pastoris 生產Candida rugosa 脂肪酶第三型的純化與性質分析及其在廢報紙脫墨上之應用。國立台灣大學微生物與生化學研究所碩士論文。 劉明啟，孫建義，翁曉燕，高慧。2006。重組畢赤酵母產木聚糖酶條件的優化。浙江大學學報 (農業與生命科學版) 32 (2) : 222 - 226。 Antonian, E. 1988. Recent advances in the purification, chracterization and structure determination of lipases. Lipids 23: 1101-1106. Benjamin, S. and A. Pandey. 1998. Candida rugosa lipases: molecular biology and versatility in biotechnology. Yeast 14 : 1069 -1087. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254 Brady, L., A. M. Brzozowski, Z. S. Derewenda, E. Dodson, G. Dodson, S. Tolley, J. P. Turkenburg, L. Christiansen, B. Huge-Jensen, L. Norskov, L. Thim and U. Menge. 1990. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343 : 767 – 770. Brocca, S., R. Grandori, D. Breviario, and M. Lotti.1995. Localization of lipase genes on Candida rugosa chromosomes. Curr. Genet. 28:454-457. Brocca, S., C. Schmidt-Dannert, M. Lotti, L. Alberghina, and R. D. Schmid. 1998. Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase. Protein Sci. 7:1415-1422. Brocca, S., F. Secundo, M. Ossola, L.Alberghina, G. Carrea ,and M. Lotti. 2003. Sequence of the lid affects activity and specificity of Candida rugosa lipase isoenzymes. Protein Sci. 12:2312-2319. Brzozowski, A. M., U. Derewenda, Z. S. Derewenda, G. G. Dodson, D. M. Lawson, J. P. Turkenburg, F. Bjorkling, B. Huge-Jensen,S.A. Patkar and L. Thim. 1991. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Nature 351: 491 – 494. Cereghino, J.L., and J.M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:45-66. Chapus, C., M. SCmCriva, C. Bovier-Lapierre and P. Desnuelle. 1976. Mechanism of pancreatic lipase action. 1. interfacial activation of pancreatic lipase. Biochemistry 15:4980-4987. Chang, S. W., G. C. Lee, and J. F. Shaw. 2006. Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase. J Agric Food Chem. 54:815-822. Clare, J.J., F. B. Rayment, S. P. Ballantine, K. Sreekrishna and M. A. Romanos.1991. High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnol. 9: 455-460. Cordle, R.A. and M. E. Lowe. 1998. Purification and characterization of human procolipase expressed in yeast cells. Protein Expr. Purif. 13: 30-35. Couderc, R., and J. Baratti. 1980. Oxidation of methanol by the yeast Pichia pastoris : Purification and properties of alcohol oxidase. Agric. Biol. Chem. 44: 2279-2289. Cregg, J.M., K.R. Madden, K.J. Barringer, G.P. Thill, and C.A. Stillman. 1989. Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol Cell Biol. 9:1316-23. Derewenda, Z.S. and A. M. Sharp.1993. News from the Interface: the molecular structures of triacylglyceride lipases. Trends Biochem Sci. 18:20-25. Diczfalusy, M. A., U. Hellman, and S. E. H. Alexson. 1997. Isolation of carboxylester lipase (CEL) isoenzymes from Candida rugosa and identification of the corresponding genes. Arch. Biochem. Biophys. 348:1-8. Dominguez de Maria, P., J.M. Sanchez-Montero, J.V. Sinisterra, and A.R. Alcantara. 2006. Understanding Candida rugosa lipases: an overview. Biotechnol Adv. 24:180-96. Dubois, M., K. A. Gilles, J. K. Hamilton, P.A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356. Dziezak, J. D.1986. Enzyme modification of dairy products. Food Technol. 4:114- 120. Ellis, S. B., P. F. Brust, P. J. Koutz, A. F. Waters, M. M. Harpold, and T. R. Gingeras. 1985. Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast, Pichia pastoris. Mol Cell. Biol. 5:1111-1121. Essamri, M., V. Deyris, and L. Comeau.1998.Optimization of lipase production by Rhizopus oryzae and study on the stability of lipase activity in organic solvents. J. Biotechnol. 60: 97–103 Gordillo, M.A., N. Obradors, J.L. Montesinos, F. Valero, J. Lafuente, and C. Sola. 1995. Stability studies and effect of the initial oleic acid concentration on lipase production by Candida rugosa. Appl Microbiol Biotechnol. 43:38-41. Grochulski, P., Y. Li, J.D. Schrag, F. Bouthillier, P. Smith, D. Harrison, B. Rubin, and M Cygler. 1993. Insights into interfacial activation from an open structure of Candida rugosa lipase. J. Biol. Chem., 268: 12843-12847. Haraldsson, G.G., P.A. Höskuldsson, S.T. Sigurdsson, F. Thorsteinsson, and S. Gudbjarnason. 1989. The preparation of triglycerides highly enriched with ω-3 polyunsaturated fatty acids via lipase catalyzed interesterifiacation. Tetrahedron Lett. 30:1671–1674. Hernáiz, M. J., J. M. Sánchez-Montero, and J. V. Sinisterra . 1997. Influence of the nature of modifier in the enzymatic activity of chemical modified semipurified lipase from Candida rugosa. Biotechnol. Bioeng. 55:252-260. Higgins, D.R. and J. M. Cregg. 1998. Methods in Molecular Biology : Pichia protocols. 107-119. Humana Press Inc. Totowa New Jersey, USA. Hitzeman, R. A., F. E. Hagie, H. L. Levine, D. V. Goeddel, G. Amerer and B. D. Hall. 1981. Expression of a human gene for interferon in yeast. Nature 293:717-722 Holmquist, M., D. C. Tessier and M. Cygler. 1997. High-level production of recombinant Geotrichum candidum lipases in yeast Pichia pastoris. Protein Expr. Purif. 11:35-40. Jaeger, K.E., B. W. Dijkstra and M. T. Reetz. 1999. Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases. Annu. Rev. Microbiol.53:315 - 351. Jaeger, K.E., S. Ransac, B.W. Dijkstra, C. Colson, M. van Heuvel, and O. Misset. 1994. Bacterial lipases. FEMS Microbiol Rev. 15:29-63. Jahic, M., M. Gustavsson, A. K. Jansen, M. Marinelle and S. O. Engors. 2003. Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J. Biotechnol. 102: 45-53. Kakugawa, K., M. Shobayashi, O. Suzuki, T. Miyakawa. 2002. Cloning, characterization, and expression of cDNA encoding a lipase from kurtzmanomyces sp. I-11. Bisci. Biotechnol. Biochem. 66(6): 1328-1336. Kawaguchi, Y., H. Honda, J. Taniguchi-Morimura, and S. Iwasaki. 1989. The codon CUG is read as serine in an asporogenic yeast Candida cylindracea. Nature 341: 164 - 166. Koutz, P., G.R. Davis, C. Stillman, K. Barringer, J. Cregg, and G. Thill. 1989. Structural comparison of the Pichia pastoris alcohol oxidase genes. Yeast. 5:167-177. Lee, G., L. Lee., V. Sava and J. Shaw. 2002. Multiple mutagenesis of non-universal serine codons of the Candida rugosa lip2 gene and biochemical characterization of purified recombinant lip2 lipase overexpressed in Pichia pastoris. Biochem. J. 366: 603-611. Lemontt, J. F., C.M. Wei, and W.R. Dackowski. 1985. Expression of active human uterine tissue plasminogen activator in yeast. DNA. 4:419-428. Linko, Y. and X. Y. Wu. 1996. Biocatalytic production of useful esters by two forms of lipase from Candida rugosa. J. Chem. Tech. Biotechnol. 65:163-170. Longhi, S., F. Fusetti, R. Grandori, M. Lotti, M. Vanoni and L. Alberghina .1992. Cloning and nucleotide sequences of two lipase genes from Candida cylindracea. Biochem. Biophys. Acta 1131:227-232. Lotti, M., R. Grandori, F. Fusetti, S. Longhi, S. Brocca, A. Tramontano and L. Alberghina.1993. Cloning and analysis of Candida cylindracea lipase sequences. Gene 124:45-55. Lotti, M., S. Brocca, F. Fusetti, and L. Alberghina.1994.Expression of recombinant Candida rugosa lipase. Med. Fac. Landbouww Univ. Gent. 59:2313-2319. Macrae, A. R. 1983. Lipase-catalyzed interesterification of oils and fats. J. Am. Oil Chem. Soc. 60:291-294. Miller, C., H. Ausrin, L. Posorske and J. Gonzlez. 1988. Characteristics of an immobilized lipase for the commercial synthesis of esters. J. Am. Oil Chem. Soc. 65 : 927-931. Mukherjee, K.D. 1994. Plant lipases and their application in lipid biotransformations. Prog Lipid Res. 33:165-174. Murasugi, A., Y. Asami and Y. Mera-Kikuchi. 2001. Production of recombinant human bile salt-stimulated lipase in Pichia pastoris. Protein Expr Purif. 23: 282-288. Nardini, M., and B.W. Dijkstra. 1999. Alpha/beta hydrolase fold enzymes: the family keeps growing. Curr Opin Struct Biol. 9:732-737. Ogata, K., H. Nishikawa ,and M. Ohsugi. 1969. A yeast capable of utilizing methanol. Agric. Biol.Chem. 33:1519-1520. Pal, Y., A. Khushoo, and K.J. Mukherjee. 2006. Process optimization of constitutive human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture. Appl Microbiol Biotechnol. 69:650-657. Paasonen, P.K. 1967. On the extractives of birch wood and brich sulphate pulp. Pap Puu 49:3-15. Plou, F.J., P. Sogo, M. V. Calvo, F. J. Burguillo and A. Ballesteros. 1997. Kinetic and enantioselective behaviour of isoenzymes A and B from Candida rugosa lipase in the hydrolysis of lipids and esters. Biocatal. Biotransform. 15:75-89. Quyen, D.T., C. Schmidt-Dannert, and R.D. Schmid. 2003. High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase. Protein Expr Purif. 28:102-110. Ramchuran, S.O., V.A. Vargas, R. Hatti-Kaul, and E.N. Karlsson. 2006. Production of a lipolytic enzyme originating from Bacillus halodurans LBB2 in the methylotrophic yeast Pichia pastoris. Appl Microbiol Biotechnol. 71:463-472. Roggenkamp, R., Z. Janowicz, B. Stanikowski, and C.P. Hollenberg. 1984. Biosynthesis and regulation of the peroxisomal methanol oxidase from the methylotrophic yeast Hansenula polymorpha. Mol Gen Genet. 194:489-493. Romanos, M.A., F.J. Hughes, S.A. Comerford, and C.A. Scorer. 1995. Production of a phosphorylated GST::HPV-6 E7 fusion protein using a yeast expression vector and glutathione S-transferase fusions. Gene. 152:137-138. Rotticci-Mulder, J.C., M. Gustavsson, M. Holmquist, K. Hult and M. Martinelle . 2001. Expression in Pichia pastoris of Candida antarctica lipase B and lipase B fused to a cellulose-binding domain. Protein Expr Purif. 3:386-392. Roukas, T. 1998. Pretreatment of beet molasses to increase pullulan production. Pro. Biochem. 33:803-810. Rúa, M.L., T. Díaz-Mauriño, V. M. Fernández, C. Otero, and A. Ballesteros .1993. Purification and characterization of two distinct lipases from Candida cylindracea. Biochim. Biophys. Acta 1159:181-189. Rubin, B. 1994. Grease pit chemistry exposed. Nat Struct Biol. 1: 568-572. Schrag, J. D., Y. Li, S. Wu, and M. Cygler. 1991. Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum. Nature 351: 761 - 764. Shagger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 203:368-369. Sharma, R., Y. Chisti, and U.C. Banerjee. 2001. Production, purification, characterization, and applications of lipases. Biotechnol Adv. 19:627-62. Shaw, J. F., C. H. Chang, and Y. J. Wang. 1989. Characterization of three distinct forms of lipolytic enzyme in a commercial Candida lipase preparation. Biotechnol. Lett.11:779-784. Sinha, J., B.A. Plantz, M. Inan, and M.M. Meagher. 2005. Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: case study with recombinant ovine interferon-tau. Biotechnol. Bioeng. 89:102-12. Tang, S.J., K.H. Sun, G.H. Sun, T.Y. Chang, and G.C. Lee. 2000. Recombinant expression of the Candida rugosa lip4 lipase in Escherichia coli. Protein Expr Purif. 20:308-13. Tang, S., J. Shaw, K. Sun, G. Sun, T. Chang, C. Lin, Y. Lo and G. Lee. 2001. Recombinant expression and characterization of the Candida rugosa lip4 Lipase in Pichia pastoris: comparison of glycosylation, activity, and stability. Arch. Biochem. Biophys. 387: 93-98. Tenkanen, M., H. Kontkanen, R. Isoniemi, P. Spetz, and B. Holmbom. 2002. Hydrolysis of steryl esters by a lipase (Lip 3) from Candida rugosa. Appl Microbiol Biotechnol. 60:120-127. Tomizuka, N., Y. Ota, and K. Yamada. 1966. Studies on lipase from Candida cylindracea : Part I. Purification and properties. Agric. Biol. Chem. 30:576-584. Tschopp, J.F., P.F. Brust, J.M. Cregg, C.A. Stillman, and T.R. Gingeras. 1987. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 15:3859-3876. Van Arsdell, S.W., G.L. Stetler, and J. Thorner. 1987. The yeast repeated element sigma contains a hormone-inducible promoter. Mol Cell Biol. 7:749-759. Van Den Hazel, H.B., M.C. Kiellanbrandt, and J.R. Winther. 1996. Biosynthesis and function of yeast vacuolar proteases: review. Yeast 12:1– 16. Veenhuis, M., J.P. Van Dijken, and W. Harder. 1983. The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts. Adv Microb Physiol. 24:1-82. Veeraragavan, K. and B. F. Gibbs. 1989. Detection and partial purification of two lipases from Candida rugosa. Biotechnol. Lett.5:345-348. Waterham, H.R., M.E. Digan, P.J. Koutz, S.V. Lair, and J.M. Cregg. 1997. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene. 186:37-44. Winkler, F. K. , A. D'Arcy, and W. Hunziker.1990. Structure of human pancreatic lipase. Nature 343 : 771 - 774. Wooley, P. and S. B. Petersen. 1994. Lipases : their structure, biochemistry and Application. Cambridge : Cambridge Univ. Press, pp. 103 - 110. Yadwad, V.B., O. P. Ward, and L. C. Noronha. 1991. Application of lipase to concentrate the docosahexaenoic acid (DHA) fraction of fish oil. Biotechnol. Bioeng. 38:956-959. Yang, Y., and M.E. Lowe. 1998. Human pancreatic triglyceride lipase expressed in yeast cells: purification and characterization. Protein Expr Purif. 13:36-40. Yang, J., K. Kobayashi, Y. Iwasaki, H. Nakano, and T. Yamane. 2000. In vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase. J Bacteriol. 182:295-302. Zabriskie, D.W., W. B. Armiger, D. H. Philips, and Albano. 1980. Trader's guide to fermentation media formulation. Traders Protein Inc., PA, USA. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24860 | - |
| dc.description.abstract | 使用中央研究院植物暨微生物學研究所蕭介夫教授研究室所構築之Pichia pastoris GS115/CRL3，以前人 (侯，2006) 最適化之培養條件為基礎，進一步探討Candida rugosa 脂肪酶三 (CRL3) 之最佳醱酵生產條件。在Hinton 氏瓶的探討中，於前人最適化之YPG 培養基中額外添加2 % beef extract 時，菌體濃度與酵素活性均上升，其中酵素活性達到255 U/mL，較前人搖瓶探討所得之最佳活性 (180 U/mL) 提升42 %，產量為110.2 mg/L；當再添加1.5 % 橄欖油，酵素活性再提升至295 U/mL。添加1 - 5 mM PMSF (Phenylmethylsulphonyl fluoride)、1 -5 μg/mL Pepstatin A 與 1 - 5 mM EDTA (Ethylenediaminetetraacetic acid) 等蛋白
酶抑制劑均無法有效提升酵素活性。粗酵素液在中性環境有較佳活性，且較適合保存在中性與30 oC 以下的環境中。為了降低生產成本，使用製糖副產物甘蔗糖蜜進行CRL3 生產。於7 % (以蔗糖濃度計) 甘蔗糖蜜、 0.2 % 尿素、0.15 % (v/v)之 85 % 磷酸與1.5 % (w/v) 玉米浸漬液之培養基中，25 oC、125 rpm 下，以Hinton 氏瓶震盪培養120 小時，OD600 為69.3，酵素活性為45.4 U/mL。 | zh_TW |
| dc.description.abstract | Pichia pastoris GS115/CRL3, constructed by Prof. Shaw’s lab, Institute of Plant and Microbial Biology, Academia Sinica, is used to produce Candida rugosa lipase 3 (CRL3). Based on the cultivation condition optimized by Hou (2006) in Hinton’s flask, the activity of CRL3 increases from 180 to 255 U/mL when 2 % beef extract is added as extra nitrogen source and yield of CRL3 is 110.2 mg/L, a 42 % increase over previous study. Furthermore, in the presence of 1.5 % olive oil, the activity reaches 295 U/mL. However, addition of protease inhibitors in the range of 1-5 mM PMSF, 1-5 μg/mL pepstatin A and 1-5 mM EDTA, respectively, do not improve the enzyme expression. Crude CRL3 has an optimal reaction and storage conditions at pH 7.0 and temperature below 30 oC. While a low-cost medium, composed of 7 % cane molasses (as sucrose), 0.2 % urea, 0.15 % (v/v) 85 % phosphoric acid and 1.5 % corn steep liquor, was used, OD600 of 69.3 and CRL3 activity of 45.4 U/mL were achieved in Hinton flasks at 25 oC, 125 rpm for 120 hours. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:57:25Z (GMT). No. of bitstreams: 1 ntu-96-R94b47104-1.pdf: 1011455 bytes, checksum: d113a1bdcb028a8b09de64926121e2e4 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 口試委員會審定書i
誌謝 ii 摘要 I Abstract II 目錄 III 圖次 V 表次VII 縮寫表VIII 第一章 前言1 第一節 脂肪酶1 第二節 Candida rugosa 脂肪酶14 第三節 嗜甲醇酵母菌Pichia 異源蛋白表現系統15 第四節 糖蜜在醱酵工業的應用25 第五節 研究動機與目的26 第六節 研究架構28 第二章 材料與方法29 第一節 使用菌株與菌株保存方法29 第二節 藥品與試劑29 第三節 儀器設備30 第四節 Hinton’s 三角瓶之培養、表現以及各種探討方法33 第五節 分析方法49 第三章 結果與討論62 第一節 YPG 培養基之組成份探討62 第二節 P. pastoris GS115/CRL3 於糖蜜培養基之三角瓶培養76 第三節 蛋白酶抑制劑作用的探討89 第四節 YPG 培養基之CRL3 粗酵素性質分析96 參考文獻102 | |
| dc.language.iso | zh-TW | |
| dc.subject | 甘蔗糖蜜 | zh_TW |
| dc.subject | Pichia pastoris | zh_TW |
| dc.subject | Candida rugosa | zh_TW |
| dc.subject | 脂肪脢 | zh_TW |
| dc.subject | 蛋白脢 | zh_TW |
| dc.subject | molasses | en |
| dc.subject | Pichia pastoris | en |
| dc.subject | Candida rugosa | en |
| dc.subject | lipase | en |
| dc.subject | protease | en |
| dc.title | 利用複合培養基與甘蔗糖蜜以 Pichia pastoris 搖瓶培養生產Candida rugosa 脂肪 | zh_TW |
| dc.title | Production of Candida rugosa lipase 3 from complex medium and molasses by Pichia pastoris in shaking flasks | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李敏雄(Min-Hsiung Lee),潘子明(Tzu-Ming Pan) | |
| dc.subject.keyword | Pichia pastoris,Candida rugosa,脂肪脢,蛋白脢,甘蔗糖蜜, | zh_TW |
| dc.subject.keyword | Pichia pastoris,Candida rugosa,lipase,protease,molasses, | en |
| dc.relation.page | 111 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2007-11-02 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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