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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李芳仁 | |
| dc.contributor.author | Meng-Ru Li | en |
| dc.contributor.author | 李孟儒 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:56:42Z | - |
| dc.date.copyright | 2011-10-05 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-08 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24808 | - |
| dc.description.abstract | The RNA binding protein, Rbp1p, was first identified as a negative regulator in Saccharomyces cerevisiae. RBP1 encodes a 672-amino acid, ~80-kD protein, which contains three RNA recognition motifs (RRM), two glutamine-rich stretches, and one asparagine-methionine-proline-rich (NMP-rich) region. Our lab previously has showed that overexpression Rbp1p RRM mutants in BY4741 induced agar-adhesion, a typical phenotype of yeast filamentous growth. In this study, we asked whether endogenous Rbp1p plays a role in filamentous growth of Σ1278b strain. Here we showed that deletion of RBP1 enhances agar adhesion, invasion and cell elongation. These phenotypes induced by rbp1 mutation depend on FLO11, which encodes a cell-wall glycoprotein regulating filamentous growth. The flo11 rbp1 double mutant fails to adhere to agar. Deletion of RBP1 significantly activates the FLO11 mRNA level, suggesting that Rbp1p negatively regulates filamentous growth through inhibition of FLO11 mRNA expression. The RRM2 motif and NMP-rich region are important for its adhesive phenotypes. Both transcription factors in MAPK pathway, Ste12p and cAMP-PKA pathway, Flo8p are required for rbp1-induced hyper-agar adhesion and FLO11 activation. Sfl1p, another transcription factor in cAMP-PKA pathway, which represses FLO11 transcripts. The FLO11 mRNA level in rbp1 sfl1 double mutant was increased much more than sfl1 mutant, implying that Rbp1p may regulate FLO11 expression in parallel with Sfl1p. Dhh1p, one of Rbp1p interacting proteins, might participate in Rbp1p-mediated adhesive growth. However, the exact mechanism for Rbp1p to regulate FLO11 expression still remains to be investigated. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:56:42Z (GMT). No. of bitstreams: 1 ntu-100-R98448010-1.pdf: 13251951 bytes, checksum: 2284a9b378f6e82489e7c37658db81c3 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | Table of Contents I
中文摘要 III Abstract IV Introduction 1 Materials and Methods 8 Results 14 Discussion 21 Figures 26 Figure 1. Deletion of RBP1 enhance agar adhesion and cell elongation in Σ1278b 26 Figure 2. rbp1 mutant strain invades agar more efficiently upon glucose starvation. 27 Figure 3. The hyper-agar adhesive growth caused by the rbp1 mutant was depend on FLO11 28 Figure 4. Deletion of RBP1 activates FLO11 expression during glucose 29 Figure 5. Construct of integrated Rbp1p, Rbp1p-rrm2, and Rbp1p-ΔNMP 30 Figure 6. Expression of wild-type Rbp1p, but not rrm2 or ΔNMP restores rbp1-induced hyper-adhesive growth 31 Figure 7. MAPK and PKA pathways are both required for rbp1-induced hyper-adhesion and FLO11 activation 32 Figure 8. Hyper-adhesion and FLO11 activation of rbp1 mutant are partially suppressed by deletion of DHH1 33 Figure 9. Construct of integrated Dhh1p, Dhh1p-ΔC81 and Dhh1p-ΔC106 34 Figure 10. Dhh1p-ΔC81 partially restores agar adhesion of the dhh1 mutant 35 Figure 11. Dhh1p-ΔC81 complements the temperature sensitive growth of the dhh1 mutant strain at 37℃ 36 Figure 12. Adhesion induced by overexpression of Rbp1-rrm2 in BY4741 is interaction-independent with Dhh1p 37 Figure 13. Sequence alignment of Rbp1p and its homolog in Candida albicans 38 Table 1. Candidate genes regulated by Rbp1p in Σ1278b 39 Table 2. Yeast strains used in this study 43 Table 2. Primers used in this study 44 Table 3. A brief summary of plasmids used in this study 46 Table 4. Antibodies used in this study 47 References 48 | |
| dc.language.iso | en | |
| dc.subject | 絲狀生長 | zh_TW |
| dc.subject | 核醣核酸蛋白 | zh_TW |
| dc.subject | filamentous growth | en |
| dc.subject | RNA-binding protein | en |
| dc.subject | Rbp1p | en |
| dc.title | 探討酵母菌核醣核酸蛋白Rbp1p對絲狀生長之調控 | zh_TW |
| dc.title | Characterization of Rbp1p-mediated filamentous growth | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄧述諄,林敬哲,羅?升 | |
| dc.subject.keyword | 核醣核酸蛋白,絲狀生長, | zh_TW |
| dc.subject.keyword | RNA-binding protein,Rbp1p,filamentous growth, | en |
| dc.relation.page | 54 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2011-08-08 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
| 顯示於系所單位： | 分子醫學研究所 | |
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