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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 葉信宏 | |
dc.contributor.author | Yu-Tzu Liu | en |
dc.contributor.author | 劉于滋 | zh_TW |
dc.date.accessioned | 2021-06-08T05:35:26Z | - |
dc.date.copyright | 2005-02-02 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-01-27 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24659 | - |
dc.description.abstract | 香蕉萎縮病係由香蕉萎縮病毒(banana bunchy top virus, BBTV)所引起,在香蕉植株上造成葉片邊緣黃化、葉脈透化、中肋及葉柄處產生濃綠色條斑、嚴重者甚至不結果等病徵。目前研究BBTV之基因體組成已發現至少六條DNA components,分別為DNA1 ~ 6,在某些BBTV分離株上甚可分離到額外的DNA components (additional Rep),根據病徵及利用PCR三對引子對(C1、S、SR)之檢測結果可將BBTV分成五種系統(type I ~ V)。然而在台灣,不同BBTV系統之完整基因體組成尚未被報導。本研究針對BBTV不同系統之分離株,利用保守性及專一性引子對,對BBTV不同系統分離株之香蕉總核酸進行PCR反應分析,增幅出可能具有的基因體,建立出PCR library,再利用restriction fragment length polymorphism (RFLP)對PCR library內之選殖株進行分析、選殖並定序來自不同RFLP群之DNA components,從type I-1與II-1兩分離株當中找到六個DNA components,並發現兩者之間除DNA2a外,各DNA components全長序列、major common region (CR-M)與stem-loop common region (CR-SL)相似度極高,主要差異在SR引子對產物(即additional Rep)之有無,進一步分析其中所含有之additional Rep種類並加以分群,同一type不同additional Rep群之間病徵並無顯著差異,對於影響type I與II之間病徵強弱之差異仍需進一步研究;從type III分離株當中只找到兩個序列特異性之DNA components (L1與L2),且無發現任何已知additional Rep的存在,其中L1為本研究中新發現之DNA component;type IV分離株只找到DNA1 ~ 5,並發現部分DNA1 與DNA3 ~ 5之CR-SL序列具有缺失,而DNA2則具有特異性CR-SL序列,並從type IV分離株發現一新的additional Rep (Ly1)。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-08T05:35:26Z (GMT). No. of bitstreams: 1 ntu-94-R89628131-1.pdf: 25934977 bytes, checksum: 95dd85d17905e328a284ae1b7527e024 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目次
、前 研究................................................................................................................1 ( ) 香蕉萎縮毒病簡介................................................................................1 ( ) BBTV基因體之研究..............................................................................2 ( ) BBTV病毒系統之研究..........................................................................9 、材料與方法............................................................................................................9 ( ) 植物材料..............................................................................................11 ( ) 總核酸抽取..........................................................................................11 ( ) 熱循環反應..........................................................................................11 1. C1及SR引子對........................................................................11 2. S引子對....................................................................................12 3. Y、S1、S2、S3之各專 性引子對.......................................12 4. 其他各DNA專 性引子對…................................................12 ( ) 核酸之選殖..........................................................................................12 ( ) 製備大腸桿菌Top10F’或XL1-Blue勝任細胞...................................12 ( ) 大腸桿菌Top10F’或XL1-Blue勝任細胞之轉形...............................13 ( ) 菌落熱循環反應..................................................................................13 ( ) 質體少量製備......................................................................................13 ( ) DNA定序.............................................................................................14 ( ) DNA序列分析.....................................................................................14 、結果......................................................................................................................15 ( ) BBTV各系統主要基因體之選殖與分析方法....................................15 ( ) BBTV各系統分離 | |
dc.language.iso | zh-TW | |
dc.title | 香蕉萎縮病毒系統之基因體研究 | zh_TW |
dc.title | Genome characterization of strains of Banana bunchy top virus | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蘇鴻基,黃新川,洪挺軒 | |
dc.subject.keyword | 香蕉,香蕉萎縮病毒,基因體研究, | zh_TW |
dc.subject.keyword | genome study,banana,banana bunchy top virus, | en |
dc.relation.page | 195 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2005-01-27 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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