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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王亞男 | |
dc.contributor.author | Hong-Chyi Jhou | en |
dc.contributor.author | 周宏祈 | zh_TW |
dc.date.accessioned | 2021-06-08T05:24:45Z | - |
dc.date.copyright | 2005-07-29 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-23 | |
dc.identifier.citation | 田中秀夫、高山貞策、真也佳博、林隆久、豬口雅彥,1992,植物細胞工學p 261-320
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24404 | - |
dc.description.abstract | 喜樹(Camptotheca acuminate Decaisne)富含camptothecin與10-Hydroxycamptothecin等生物鹼成分。
以胚為培殖體誘導癒合組織結果顯示:胚在MS基礎培養基中添加IBA 0.5mg/l與BA 4mg/l、 Picloram 0.5mg/l與Kinetin 4mg/l、NAA 4mg/l與BA 2mg/l及單獨添加NAA 1mg/l之處理下,可以得到較佳的癒合組織生長,其中以NAA 4mg/l與BA 2mg/l組合所誘導出癒合組織較為鬆軟且生長快速,可作為懸浮培養試驗的材料。在多芽體誘導方面,以MS基礎培養基添加IBA 0.5 mg/l與BA 0.5mg/l的誘導效果最佳。根的誘導則以MS基礎培養基添加IBA 2 mg/l效果最佳。 利用喜樹癒合組織細胞建立懸浮培養系統,選擇salicylic acid、chitin、jasmonic acid、methyl jasmonate等四種誘發劑,來探討其10-hydroxycamptothecin生產的影響。添加100μM salicylic acid於MS液態培養基中,經7天培養後10-Hydroxycamptothecin產量887.5μg/g。而加入chitin懸浮3ml/25ml至MS液態培養基中時,10-Hydroxycamptothecin含量可達2502.7μg/g效果較佳。另外jasmonic acid濃度為100μM10-Hydroxycamptothecin之含量可達10246μg/g,為四種誘發劑當中效果最為顯著。而當methyl jasmonate濃度為200μM時,10-Hydroxycamptothecin之含量可達3836.6μg/g。 | zh_TW |
dc.description.abstract | The happy tree (Camptotheca acuminate Decaisne) is one of the plants containing many alkloids, famous as camptothecin and 10-hydroxycamptothecin.
An experiment of embryo in vito was conducted to induce callus from the happy tree. The results showed that the callus grew well under all MS medium treatments of 0.5 mg/l IBA + 4 mg/l BA, 0.5 mg/l picloram + 4 mg/l kinetin, 4 mg/l NAA + 2 mg/l BA, and 1 mg/l NAA, However, the callus obtained from the treatment of 4 mg/l NAA + 2 mg/l BA was soft and facilitated to be used as a material for the suspension culture experiment. In the multiple buds induce culture, the best result was obtained on the MS medium adding 0.5 mg/l IBA + 0.5 mg/l BA. The adventitious roots occurred best on the MS medium with 2 mg/l IBA in the culture. Four elicitor of salicylic acid, chitin, jasmonic acid and methyl jasmontate were selected to investigate 10-hydroxycamptothecin production from the happy tree using obtained callus suspension culture. The best productions of 10-hydroxycamptothecin were 887.5, 2579.5, 10246, 3836.6μg/g under the treatments of 100 μM salicylic acid, 3 ml/25ml chitin, 100 μM jasmocin acid and 200 μM methyl jasmonate, respectively, after a week incubation. Obviously, jasmonic acid has the best effect in inducing 10-hydroxycamptothecine production from the callus suspension culture. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T05:24:45Z (GMT). No. of bitstreams: 1 ntu-94-R90625040-1.pdf: 4591980 bytes, checksum: 71746695a133d039a22bfba62ab7ec41 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目錄………………………………………………………………………Ⅰ
表目錄……………………………………………………………………Ⅱ 圖目錄……………………………………………………………………Ⅴ 中文摘要…………………………………………………………………Ⅵ 英文摘要…………………………………………………………………Ⅶ 縮寫字……………………………………………………………………Ⅷ 壹、前言…………………………………………………………………1 貳、前人研究……………………………………………………………2 一、喜樹之分布、形態及藥理成分……………………………………2 二、植物二次代謝物……………………………………………………6 三、植物組織培養生產二次代謝物……………………………………8 參、材料與方法…………………………………………………………16 一、材料…………………………………………………………………16 二、方法…………………………………………………………………16 三、喜樹鹼測定…………………………………………………………21 肆、結果與討論…………………………………………………………24 一、喜樹組織養 ………………………………………………………24 二、喜樹鹼成份測定……………………………………………………34 伍、結論…………………………………………………………………61 陸、參考文獻……………………………………………………………63 柒、照片…………………………………………………………………68 捌、附錄…………………………………………………………………82 | |
dc.language.iso | zh-TW | |
dc.title | 喜樹組織培養與喜樹鹼含量之研究 | zh_TW |
dc.title | Tissue Culturesof Camptotheca acuminate Decaisne and Camptothecin contents | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李明仁,廖宇賡,蕭英倫,林敏宜 | |
dc.subject.keyword | 喜樹,組織培養,喜樹鹼, | zh_TW |
dc.subject.keyword | Camptotheca acuminate Decaisne,tissue cultures,camptothecin, | en |
dc.relation.page | 92 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2005-07-24 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 森林學研究所 | zh_TW |
顯示於系所單位: | 森林環境暨資源學系 |
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