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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡懷楨 | |
| dc.contributor.author | Tsung-Che Hsieh | en |
| dc.contributor.author | 謝宗哲 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:21:56Z | - |
| dc.date.copyright | 2005-07-29 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-26 | |
| dc.identifier.citation | Cueva-Mendez, G., D.Mills, A., Clay-Farrace, L., Diaz-Orejas, R. and A.Laskey, R. (2003). Regulatable killing of eukaryotic cells by the prokaryotic proteins Kid and Kis. EMBO J. 22, 246-251
Devreotes, P. and Janetopoulos, C. (2003). Eukaryotic chemotaxis:distinctions between directional sensing and polarization. J. Biol. Chem. 278, 20445-20448 Doitsidou, M., Reichman-fried, M., Stebler, J., Koprunner, M., Dorries, J., Meyer, D., Esguerra, C.V., Leung, T., and Raz, E. (2002). Guidance of primordial germ cell migration by the chemokine SDF-1. Cell 111, 647-659 Furth, P.A., St-Onge, L., Boger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H. and Hennighausen, L. (1994). Temporal control of gene expression in transgenic mice by a tetracycline- responsive promoter. Proc. Natl. Acad. Sci. USA 91, 9302-9306 Gossen, M. and Bujeard, H. (1992). Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89, 5547-5551 Gossen, M., Freundlieb, S., Bender, G., Muller, G., Hillen, W. and Bujard, H. (1995). Transcriptional activation by tetracyclines in mammalian cells. Science 268, 1766-1769 Horvitz, H.R., and Herskowitz, I. (1992). Mechanisms of asymmetric cell division: two Bs or not two Bs, that is the question. Cell 68, 237–255 Horwitz, R., and Webb, D. (2003). Cell migration. Curr. Biol. 13, 756-759 Huang, C.J., Jou, T.S., Lee, W.H., Jeng, Y.T., Ho, Y.L., Hsieh, F.J. and Tsai, H.J. (2005). Conditional expression of myocardium-specific transgene in the zebrafish transgenic line. Dev. Dyn. inpress Iijima, M., Huang, Y. E. and Devreotes, P. (2002). Temporal and spatial regulation of chemotaxis. Dev. Cell 3, 469-478 Inoue, H., Nojima, H., Okayama, H. (1990). High efficiency transformation of Escherichia coli with plasmids. Gene 96, 23-28 Jensen, R.B. and Gerdes, K. (1995). Programmed cell death in bacteria : proteic plasmid stabilization systems. Mol. Microbiol. 17, 205-210 Jost, M., Kari, C. and Rodeck, U. (1997). An episomal vector for stable tetracycline-regulated gene expression. Nucleic Acids Res. 25, 3131-3134 Kistner, A., Gossen, M., Zimmermann, F., Jerecic, J., Ullmer, C., Lubbert, H. and Bujard, H. (1996). Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc. Natl. Acad. Sci. USA 93, 10933-10938 Knaut, H., Pelegri, F., Bohmann, K., Schwarz, H. and Nusslein-Volhard, C. (2000). Zebrafish vasa RNA but not Its protein is a component of the germ plasm and segregates asymmetrically before gremline specification. J. Cell Biol. 149, 875-888 Knaut, H., Steinbeisser, H., Schearz, H. and Nusslein-Volhard, C. (2002). An evolutionary conserved region in the vasa 3’utr targets RNA translation to the germ cells in the zebrafish. Curr. Biol. 12, 454-466 Koprunner, M., Thisse, C., Thisse, B. and Raz, E. (2001). A zebrafish nanos-related gene is essential for the development of primordial germ cells. Genes Dev. 15, 2877-2885 Krovel, A.V. and Olsen, L.C. (2002). Expression of a vas::EGFP transgene in primordial germ cells of the zebrafish. Mech. Dev. 116, 141–150 Lee, Y.H. (2003). Conditional expression in the zebrafish heart with tet-on regulatory system. Master thesis, National Taiwan University, Taipei, Taiwan Meissner, M., Brecht, S., Bujard, H. and Soldati, D. (2001). Modulation of Myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii. Nucleic Acids Res. 29, e115 Nakayama, H., Mio, T., Nagahashi, S., Kokado, M. and Arisawa, M. (2000). Tetracycline- regulatable system to tight control gene expression in the pathogenic fungus Candida aibicans. Infect Immun. 68, 6712-6719 Passman, R.S. and Fishman, G.I. (1994). Regulated expression of foreign genes in vivo after germline transfer. J. Clin. Invest. 94, 2421-2425 Raz, E. (2003). Primordial germ-cell development: the zebrafish perspective. Nat. Rev. Genet. 4, 690-700 Salucci, V., Scarito, A., Aurisicchio, L., Lamartina, S., Nicolaus, G., Giampaoli, S., Gonzalez-Paz, O., Toniatti, C., Bujard, H., Hillen, W., Ciliberto, G. and Palombo, F. (2002). Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2S-M2 tetracycline transactivator and resressor system. Gene Ther. 9, 1415-1421 Schulte-Merker, S., Lee, K.J., McMahon, A.P. and Hammerschmidt, M. (1997). The zebrafish organizer requires chordino. Nature 387, 862–863. Seydoux, G. and Strome, S. (1999). Launching the germline in Caenorhabditis elegans: regulation of gene expression in early germ cells. Development 126, 3275–3283 Slanchev, K., Stebler, J., Cueva-Mendez, G. and Raz, E. (2005). Development without germ cells: the role of the germ line in zebrafish sex differentiation. Proc. Natl. Acad. Sci. USA 102, 1074-1079 Starz-Gaiano, M. and Lehmann, R. (2001). Moving towards the next generation. Mech. Dev. 105, 5–18 Wang, C.L. (2004). Knock down the expression of zebrafish cardiac troponin C in heart by conditionally generating antisense strand in vivo. Master thesis, National Taiwan University, Taipei, Taiwan Weidinger, G., Stebler, J., Slanchev, K., Dumstrei, K., Wise, C., Lovell-Badge, R., Thisse, C., Thisse, B. and Raz, E. (2002). dead end, a novel vertebrate germ plasm component, is required for zebrafish primordial germ cell migration and survival. Curr. Biol. 13, 1429-1434 Weidinger, G., Wolke, U., Koprunner, M., Klinger, M. and Raz, E. (1999). Identification of tissues and patterning events required for distinct steps in early migration of zebrafish primordial germ cells. Development 126, 5295-5307 Weidinger, G., Wolke, U., Koprunner, M., Thisse, C., Thisse, B. and Raz, E. (2002). Regulation of zebrafish primordial germ cell migration by attraction towards an intermediate target. Development 129, 25-36 Weinmann, P., Gossen, M., Hillen, W., Bujard, H. and Gatz, C. (1994). A chimeric transactivator allows tetracycline-responsive gene expression in whole plants. Plant J. 5, 559-569 Wylie, C. (2000). Germ cells. Curr. Opin. Genet. Dev. 10, 410-413 Yang, J., Zhou, W., Zhang, Y., Zidon, T., Ritchie, T. and Engelhardt, J.F. (1999). Concatamerization of adeno-associated virus circular genomes occurs through intermolecular recombination. J. Virol. 73, 9468-9477 Yoon, C., Kawakami, K. and Hopkins, N. (1997). Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells. Development 124, 3157–3165 Yu, Z., Redfern, C.S. and Fishman, G.I. (1996). Conditional transgene expression in the heart. Circ. Res. 79, 691-697 Zhu, Z., Zheng, T., Lee, C.G., Homer, R.J. and Elias, J.A. (2002). Tetracycline-controlled transcriptional regulation system: advances and application in transgenic animal modeling. Cell. Dev. Biol. 13, 121-128 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24323 | - |
| dc.description.abstract | 在斑馬魚胚胎發育4 cells至1000 cells時，生殖母細胞(Primordial Germ Cells, PGCs)在胚胎裡形成4群，之後會經由遷移，到達將來成體生殖腺發育的位置，經減數分裂產生配子，負責把親代的遺傳訊息傳遞到下一代。本實驗構築了pZBrBISRK質體。將此構築好的質體，以BstBI線性化，經顯微注射後，篩選出5株轉殖魚品系，並挑選了其中遺傳率(~45%)較高的ZBrBISRK2轉殖品系。在ZBrBISRK2的F2胚胎，在30 hpf時，在個體的頭部、軀幹以及尾部可以偵測到異位性nanos mRNA的表現，然而在野生型控制組中，nanos mRNA僅僅在gonad的位置有表現。進一步地，在Western blot的實驗中，能夠偵測到下游質體蛋白質專一的訊號。藉由以上的證據可知，利用誘導式調控系統確實造成PGCs在遷移時受到影響而產生異位性的遷移。 | zh_TW |
| dc.description.abstract | In embryogenesis of zebrafish, primordial germ cells (PGCs) formed four clumps before 1000-cell stage. PGCs would migrate and form two bilateral rows of cells close to the future gonad site. We micro-injected with a BstBI-cut pZBrBISRK plasmid and generated 5 transgenic line. One of which, ZBrBISRK2, having 45 % heredity rate, the highest among 5 transgenic lines, was selected. The nanos mRNA was detected not only in future gonad but also in head, trunk, and tail in the F2 embryos at 30 hpf derived from transgenic line ZBrBISRK2, whereas nanos mRNA was only detected in the gonad of wild type embryos, suggesting there were the mis-migrated PGCs. In Western blot, we could detect the positive bands in the total proteins extracted from ZBrBISRK2 F2 embryos. Therefore, we conclude that using conditionally regulatory system results in interfering the PGCs migration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:21:56Z (GMT). No. of bitstreams: 1 ntu-94-R92b45021-1.pdf: 1684279 bytes, checksum: 7c5f54179085de574215e76e7d8710d5 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 中文摘要 ---------------------------------------------------------- 1
英文摘要 ---------------------------------------------------------- 2 壹、前言 ---------------------------------------------------------- 3 貳、實驗材料 ---------------------------------------------------- 11 參、實驗方法 ---------------------------------------------------- 15 肆、結果 ---------------------------------------------------------- 27 伍、討論 ---------------------------------------------------------- 31 陸、參考資料 ---------------------------------------------------- 38 圖表 ---------------------------------------------------------------- 44 附錄 ---------------------------------------------------------------- 50 | |
| dc.language.iso | zh-TW | |
| dc.subject | 斑馬魚 | zh_TW |
| dc.subject | zebrafish | en |
| dc.title | 利用可誘導式調控系統影響斑馬魚生殖母細胞的遷移 | zh_TW |
| dc.title | Conditionally interfere the migration of zebrafish (Danio rerio) Primordial Germ Cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 宣大衛,蔡錦玲,羅秀婉 | |
| dc.subject.keyword | 斑馬魚, | zh_TW |
| dc.subject.keyword | zebrafish, | en |
| dc.relation.page | 58 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2005-07-26 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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