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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24269完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許瑞祥(Ruey-Shyang Hseu) | |
| dc.contributor.author | Chih-Ying Lay | en |
| dc.contributor.author | 賴志穎 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:20:16Z | - |
| dc.date.copyright | 2005-07-28 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-27 | |
| dc.identifier.citation | 參考文獻
1. Adaskaveg, J. E., R. L. Gilbertson, and R. A. Blanchette. 1990. Comparative studies of delignification caused by Ganoderma species. Appl Environ Microbiol 56:1932-1943. 2. Adaskaveg, J. E., and R. L. Gilbertson. 1994. Wood decay caused by Ganoderma species in the G. lucidum complex. Proceedings of Contributed Symposium 59A, B:79-93. 3. Aggelis, G., C. Ehaliotis, F. Nerud, I. Stoychev, G. Lyberatos, and G. I. Zervakis. 2002. Evaluation of white-rot fungi for detoxification and decolorization of effluents from the green olive debittering process. Appl Microbiol Biotechnol 59:353-60. 4. Ahuja, S. K., G. M. Ferreira, and A. R. Moreira. 2004. Utilization of enzymes for environmental applications. Crit Rev Biotechnol 24:125-54. 5. Alexopoulos, C. J., C. W. Mims, and M. Blackwell. 1996. Introductory Mycology. John Wiley & sons, Inc. 4th edition. 6. Al-Samarrai, T. H., and J. Schmid. 2000. A simple method for extraction of fungal genomic DNA. Lett Appl Microbiol 30:53-6. 7. Austin, S., E. T. Bingham, D. E. Mathews, M. N. Shahan, J. Will, and R. R. Burgess. 1995. Production and field performance of transgenic alfalfa (Medicago sativa L.) expression alpha-amylase and manganese-dependent lignin peroxidase. Euphytica 85:381-93. 8. Bai, Z., L. M. Harvey, and B. McNeil. 2003. Oxidative stress in submerged cultures of fungi. Crit Rev Biotechnol 23:267-302. 9. Bajpai, P. 2004. Biological bleaching of chemical pulps. Crit Rev Biotechnol 24:1-58. 10. Baldrian, P. 2004. Purification and characterization of laccase from the white-rot fungus Daedalea quercina and decolorization of synthetic dyes by the enzyme. Appl Microbiol Biotechnol 63:560-3. 11. Belinky, P. A., N. Flikshtein, S. Lechenko, S. Gepstein, and C. G. Dosoretz. 2003. Reactive oxygen species and induction of lignin peroxidase in Phanerochaete chrysosporium. Appl Environ Microbiol 69:6500-6. 12. Bidlack, J., M. Malone, and R. Benson. 1992. Molecular structure and component integration of secondary cell walls in plants. Proc. Okla. Acad. Sci. 72:51-56. 13. Boer, C. G., L. Obici, C. G. M. de Souza, and R. M. Peralta. 2004. Decolorization of synthetic dyes by solid state cultures of Lentinula (Lentinus) edodes producing manganese peroxidase as the main ligninolytic enzyme. Bioresource Technology 94:107-112. 14. Bogan, B. W., and R. T. Lamar. 1996. Polycyclic aromatic hydrocarbon-degrading capabilities of Phanerochaete laevis HHB-1625 and its extracellular ligninolytic enzymes. Appl Environ Microbiol 62:1597-603. 15. Bogan, B. W., B. Schoenike, R. T. Lamar, and D. Cullen. 1996. Manganese peroxidase mRNA and enzyme activity levels during bioremediation of polycyclic aromatic hydrocarbon-contaminated soil with Phanerochaete chrysosporium. Appl Environ Microbiol 62:2381-6. 16. Bollag, J. M., and A. Leonowicz. 1984. Comparative studies of extracellular fungal laccases. Appl Environ Microbiol 48:849-854. 17. Boudet, A. M., S. Kajita, J. Grima-Pettenati, and D. Goffner. 2003. Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends Plant Sci 8:576-81. 18. Boyce, J. S. 1948. Forest Pathology. McGraw-Hill Book company, Inc. 2nd edition. 19. Breen, A., and F. L. Singleton. 1999. Fungi in lignocellulose breakdown and biopulping. Curr Opin Biotechnol 10:252-8. 20. Brown, J. A., J. K. Glenn, and M. H. Gold. 1990. Manganese regulates expression of manganese peroxidase by Phanerochaete chrysosporium. J Bacteriol 172:3125-30. 21. Champagne, P. P., and J. A. Ramsay. 2005. Contribution of manganese peroxidase and laccase to dye decoloration by Trametes versicolor. Appl Microbiol Biotechnol. 22. Chen, I. C., H. C. Chang, H. W. Yang, and G. L. Chen. 2004. Evaluation of total antioxidant activity of several popular vegetables and Chinese herbs: A fast approach with ABTS/H2O2/HRP system in microplates. J Food and Drug Analysis 12:29-33. 23. Cohen, R., Y. Hadar, and O. Yarden. 2001. Transcript and activity levels of different Pleurotus ostreatus peroxidases are differentially affected by Mn2+. Environ Microbiol 3:312-22. 24. Cohen, R., L. Persky, and Y. Hadar. 2002. Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl Microbiol Biotechnol 58:582-94. 25. de Koker, T. H., J. Zhao, D. Cullen, and B. J. H. Janse. 1998. Biochemical and molecular characterization of South African strains of Phanerochaete chrysosporium. Mycroi. Res. 102:88-92. 26. Dittmer, N. T., R. J. Suderman, H. Jiang, Y. C. Zhu, M. J. Gorman, K. J. Kramer, and M. R. Kanost. 2004. Characterization of cDNAs encoding putative laccase-like multicopper oxidases and developmental expression in the tobacco hornworm, Manduca sexta, and the malaria mosquito, Anopheles gambiae. Insect Biochem Mol Biol 34:29-41. 27. Dix, N. J., and J. Webster. 1995. Fungal Ecology. Chapman & Hall 1st edition. 28. Doring, F., M. Klapper, S. Theis, and H. Daniel. 1998. Use of the glyceraldehyde-3-phosphate dehydrogenase promoter for production of functional mammalian membrane transport proteins in the yeast Pichia pastoris. Biochem Biophys Res Commun 250:531-5. 29. D'Souza, T. M., K. Boominathan, and C. A. Reddy. 1996. Isolation of laccase gene-specific sequences from white rot and brown rot fungi by PCR. Appl Environ Microbiol 62:3739-44. 30. D'Souza, T. M., C. S. Merritt, and C. A. Reddy. 1999. Lignin-modifying enzymes of the white rot basidiomycete Ganoderma lucidum. Appl Environ Microbiol 65:5307-13. 31. Edwards, S. L., R. Raag, H. Wariishi, M. H. Gold, and T. L. Poulos. 1993. Crystal structure of lignin peroxidase. Proc Natl Acad Sci U S A 90:750-4. 32. Fenice, M., G. G. Sermanni, F. Federici, and A. D'Annibale. 2003. Submerged and solid-state production of laccase and Mn-peroxidase by Panus tigrinus on olive mill wastewater-based media. J Biotechnol 100:77-85. 33. Gettemy, J. M., D. Li, M. Alic, and M. H. Gold. 1997. Truncated-gene reporter system for studying the regulation of manganese peroxidase expression. Curr Genet 31:519-24. 34. Glenn, J. K., L. Akileswaran, and M. H. Gold. 1986. Mn(II) oxidation is the principal function of the extracellular Mn-peroxidase from Phanerochaete chrysosporium. Arch Biochem Biophys 251:688-96. 35. Giardina, P., G. Palmieri, B. Fontanella, V. Rivieccio, and G. Sannia. 2000. Manganese peroxidaseisozymes produced by Pleurotus ostreatus grown on wood sawdust. Arch Biochem Biophys 376:171-9. 36. Glenn, J. K., and M. H. Gold. 1985. Purification and characterization of an extracelular Mn(II)-dependent peroxidase from the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Arch Biochem Biophys 242:329-41. 37. Glumoff, T., P. J. Harvey, S. Molinari, M. Goble, G. Frank, J. M. Palmer, J. D. G. Smit, and M. S. A. Leisola. 1990. Lignin peroxidase from Phanerochaete chrysosporium: Molecular and kinetic characterization of isozymes. Eur J Biochem 187:515-20. 38. Gomez-Toribio, V., A. T. Martinez, M. J. Martinez, and F. Guillen. 2001. Oxidation of hydroquinones by the versatile ligninolytic peroxidase from Pleurotus eryngii. H2O2 generation and the influence of Mn2+. Eur J Biochem 268:4787-93. 39. Gonzalez, T., M. C. Terron, S. Yague, E. Zapico, G. C. Galletti, and A. E. Gonzalez. 2000. Pyrolysis/gas chromatography/mass spectrometry monitoring of fungal-biotreated distillery wastewater using Trametes sp. I-62 (CECT 20197). Rapid Commun Mass Spectrom 14:1417-24. 40. Gu, L., C. Lajoie, and C. Kelly. 2003. Expression of a Phanerochaete chrysosporium manganese peroxidase gene in the yeast Pichia pastoris. Biotechnol Prog 19:1403-9. 41. Have, R. T., S. Hartmans, And J. A. Field. 1997. Interference of peptone and tyrosine with the lignin peroxidase assay. Appl Environ Microbiol 63:3301-3303. 42. Hofrichter, M. 2002. Review: lignin conversion by manganese peroxidase (MnP). Enzyme and Microbial Technology 30:454-466. 43. Hofrichter, M., T. Lundell, and A. Hatakka. 2001. Conversion of milled pine wood by manganese peroxidase from Phlebia radiata. Appl Environ Microbiol 67:4588-93. 44. Honda, Y., T. Matsuyama, T. Irie, T. Watanabe, and M. Kuwahara. 2000. Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus. Curr Genet 37:209-12. 45. Huang, X., Dan Wang, Caixia Liu, Ming Hu, Yinbo Qu, and Peiji Gao. 2003. The roles of veratryl alcohol and nonionic surfactant in the oxidation of phenolic compounds by lignin peroxidase. Biochemical and Biophysical Research Communications 311:491-494. 46. Huttermann, A., C. Mai, and A. Kharazipour. 2001. Modification of lignin for the production of new compounded materials. Appl Microbiol Biotechnol 55:387-94. 47. Iimura, Y., S. Ikeda, T. Sonoki, T. Hayakawa, S. Kajita, K. Kimbara, K. Tatsumi, and Y. Katayama. 2002. Expression of a gene for Mn-peroxidase from Coriolus versicolor in transgenic tobacco generates potential tools for phytoremediation. Appl Microbiol Biotechnol 59:246-51. 48. Irie, T., Y. Honda, T. Watanabe, and M. Kuwahara. 2001. Homologous expression of recombinant manganese peroxidase genes in ligninolytic fungus Pleurotus ostreatus. Appl Microbiol Biotechnol 55:566-70. 49. Janse, B. J. H., J. Gaskell, M. Akhtar, and D. Cullen. 1998. Expression of Phanerochaete chrysosporium genes encoding lignin peroxidases, manganese peroxidases, and glyoxal oxidase in wood. Appl Environ Microbiol 64:3536-8. 50. Johansson, T., Per Olof Nyman, and Daniel Cullen. 2002. Differential regulation of mnp2, a new manganese peroxidase-encoding gene from the ligninolytic fungus Trametes versicolor PRL 572. Appl Environ Microbiol 68:2077-2080. 51. Johansson, T., and P. O. Nyman. 1996. A cluster of genes encoding major isozymes of lignin peroxidase and manganese peroxidase from the white-rot fungus Trametes versicolor. Gene 170:31-8. 52. Johnson, T. M., and J. K. Li. 1991. Heterologous expression and characterization of an active lignin peroxidase from Phanerochaete chrysosporium using recombinant baculovirus. Arch Biochem Biophys 291:371-8. 53. Kamitsuji, H., Y. Honda, T. Watanabe, and M. Kuwahara. 2004. Production and induction of manganese peroxidase isozymes in a white-rot fungus Pleurotus ostreatus. Appl Microbiol Biotechnol 65:287-94. 54. Kilstrup, M., and K. N. Kristiansen. 2000. Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer. Nucleic Acids Res 28:E55. 55. Kirk, T. K., E. Schultz, W. J. Conners, L. F. Lorenz, and J. G. Zeikus. 1978. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Arch Microbiol 117:277-85. 56. Kirk, T. K., and R. L. Farrell. 1987. Enzymatic 'combustion': the microbial degradation of lignin. Ann Rev Microbiol. 41:465-505. 57. Kondo, R., K. Harazono, K. Tsuchikawa, and K. Sakai. 1996. Biological bleaching of kraft pulp with lignin-degrading enzymes. Enzymes in Pulp and Paper Processing, ACS Symposium Series. 655.:228-40. 58. Krause, D. O., S. E. Denman, R. I. Mackie, M. Morrison, A. L. Rae, G. T. Attwood, and C. S. McSweeney. 2003. Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics. FEMS Microbiol Rev 27:663-93. 59. Ladisch, M. R., K. W. Lin, M. Voloch, and G. T. Tsao. 1983. Process considerations in the enzymatic hydrolysis of biomass. Enzyme Microb Technol 5:82-96. 60. Laemmli, U. K. 1970. Cleavage of structural proteins durinf the assembly of the head of bacteriophage T4. Nature 227:680-5. 61. Larrondo, L. F., M. Avila, L. Salas, D. Cullen, and R. Vicuna. 2003. Heterologous expression of laccase cDNA from Ceriporiopsis subvermispora yields copper-activated apoprotein and complex isoform patterns. Microbiology 149:1177-82. 62. Larrondo, L. F., S. Lobos, P. Stewart, D. Cullen, and R. Vicuna. 2001. Isoenzyme multiplicity and characterization of recombinant manganese peroxidases from Ceriporiopsis subvermispora and Phanerochaete chrysosporium. Appl Environ Microbiol 67:2070-5. 63. Larrondo, L. F., L. Salas, F. Melo, R. Vicuna, and D. Cullen. 2003. A novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity. Appl Environ Microbiol 69:6257-63. 64. Lau, K. L., Y. Y. Tsang, and S. W. Chiu. 2003. Use of spent mushroom compost to bioremediate PAH-contaminated samples. Chemosphere 52:1539-46. 65. Levin, L., L. Papinutti, F. Forchiassin. 2004. Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes. Bioresource Technology 94:169-76. 66. Li, D., Heather L. Youngs, and Michael H. Gold. 2001. Heterologous Expression of a Thermostable Manganese Peroxidase from Dichomitus squalens in Phanerochaete chrysosporium. Arch Biochem Biophys 385:348-356. 67. Li, D., M. Alic, J. A. Brown, and M. H. Gold. 1995. Regulation of manganese peroxidase gene transcription by hydrogen peroxide, chemical stress, and molecular oxygen. Appl Environ Microbiol 61:341-5. 68. Lopez, C., I. Mielgo, M. T. Moreira, G. Feijoo, and J. M. Lema. 2002. Enzymatic membrane reactors for biodegradation of recalcitrant compounds. Application to dye decolourisation. J Biotechnol 99:249-57. 69. Ma, B., M. B. Mayfield, and M. H. Gold. 2003. Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker. Curr Genet 43:407-14. 70. Machii, Y., H. Hirai, and T. Nishida. 2004. Lignin peroxidase is involved in the biobleaching of manganese-less oxygen-delignified hardwood kraft pulp by white-rot fungi in the solid-fermentation system. FEMS Microbiol Lett 233:283-7. 71. Machwe, A., Anna Marie Senczuk, Manju Kapoor. 2002. Induction profiles and properties of a novel stress-induced peroxidase in Neurospora crassa. Mycoscience 43:103-111. 72. Maijala, P. 2000. Heterobasidion annosum and wood decay: Enzymology of cellulose, hemicellulose, and lignin degradation. Doctor Thesis. Department of Biosciences, Division of Plant Physiology, University of Helsinki. 73. Maijala, P., T. C. Harrington, and M. Raudaskoski. 2003. A peroxidase gene family and gene trees in Heterobasidion and related genera. Mycologia 95:209-21. 74. Martinez, A. T., J. M. Barrasa, M. J. Martinez, G. Almendros, M. Blanco, and A. E. Gonzalez. 1994. Ganoderma australe: A fungus responsible for extensiv delignification of some austral hardwoods. Proceedings of Contributed Symposium 59A, B:67-77. 75. Martinez, A. T. 2002. Molecular biology and structure-function of lignin-degrading heme peroxidases. Enzyme and Microbial Technology 30:425-444. 76. Mayer, A. M., and R. C. Staples. 2002. Laccase: new functions for an old enzyme. Phytochemistry 60:551-65. 77. Mayfield, M. B., K. Kishi, M. Alic, and M. H. Gold. 1994. Homologous expression of recombinant manganese peroxidase in Phanerochaete chrysosporium. Appl Environ Microbiol 60:4303-9. 78. Mester, T., and Jim A. Field. 1998. Characterization of a novel manganese peroxidase-lignin peroxidase hybrid isozyme produced by Bjerkandera species strain BOS55 in the absence of manganese. J Biol Chem 273:15412-17. 79. Miller, R. N. G., M. Holderness, P. D. Bridge, R. R. M. Paterson, M. Sariah, M. Z. Hussin, and E. J. Hilsley. 1994. A multi-disciplinary approach to the characterization of Ganoderma in oil-palm cropping systems. Proceedings of Contributed Symposium 59A, B:57-66. 80. Min, K. L., Y. H. Kim, Y. W. Kim, H. S. Jung, and Y. C. Hah. 2001. Characterization of a novel laccase produced by the wood-rotting fungus Phellinus ribis. Arch Biochem Biophys 392:279-86. 81. Murrill, W. A. 1902. The Polyporaceae of North America. I. The Genus Ganoderma. New York Botanical Garden. 82. Ohkuma, M., Yoshimasa Maeda, Toru Johjima, and Toshiaki Kudo. 2001. Lignin degradation and roles of white rot fungi: Study on an efficient symbiotic system in fungus-growing termites and its application to bioremediation. RIKEN Review 42:39-42. 83. Orth, A. B., D. J. Royse, and M. Tien. 1993. Ubiquity of Lignin-degrading peroxidases among various wood-degrading fungi. Appl Environ Microbiol 59:4017-4023. 84. Pease, E. A., S. D. Aust, and M. Tien. 1991. Heterologous expression of active manganese peroxidase from Phanerochaete chrysosporium using the baculovirus expression system. Biochem Biophys Res Commun 179:897-903. 85. Pease, E. A., and M. Tien. 1991. Lignin-degrading enzymes from the filamentous fungus Phanerochaete chrysosporium. Biocatalysis for industry, I. S. Dordick edt.:115-135. 86. Pelaez, P., M. J. Martinez, and A. T. Martinez. 1995. Screening of 68 species of basidiomycetes for enzymes involved in lignin degradation. Mycol Res 99:37-42. 87. Perumal, K., and P. T. Kalaichelvan. 1996. Production of extracellular lignin peroxidase and laccase by Ganoderma lucidum PTK3 on sugarcane bagasse lignin. Indian J Exp Bio 34:1121-25. 88. Piontek, K., T. Glumoff, and K. Winterhalter. 1993. Low pH crystal structure of glycosylated lignin peroxidase from Phanerochaete chrysosporium at 2.5 Å resolution. FEBS Lett 315:119-24. 89. Podgornik, H., M. Stegu, A. Podgornik, and A. Perdih. 2001. Isolation and characterization of Mn(III) tartrate from Phanerochaete chrysosporium culture broth. FEMS Microbiol Lett 201:265-9. 90. Poulos, T. L., S. L. Edwards, H. Wariishi, and M. H. Gold. 1993. Crystallographic refinement of lignin peroxidase at 2 Å. J Biol Chem 268:4429-40. 91. Pribnow, D., M. B. Mayfield, V. J. Nipper, J. A. Brown, and M. H. Gold. 1989. Characterization of a cDNA encoding a manganese peroxidase, from the lignin-degrading basidiomycete Phanerochaete chrysosporium. J Biol Chem 264:5036-40. 92. Ruiz, J. C., T. de la Rubia, J. Perez, and J. Martinez Lopez. 2002. Effect of olive oil mill wastewater on extracellular ligninolytic enzymes produced by Phanerochaete flavido-alba. FEMS Microbiol Lett 212:41-5. 93. Ruiz-Duenas, F. J., F. Guillen, S. Camarero, M. Perez-Boada, M. J. Martinez, and A. T. Martinez. 1999. Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii. Appl Environ Microbiol 65:4458-63. 94. Ruiz-Duenas, F. J., M. J. Martinez, and A. T. Martinez. 1999. Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn(2+) and different aromatic substrates. Appl Environ Microbiol 65:4705-7. 95. Sack, U., M. Hofrichter, and W. Fritsche. 1997. Degradation of polycyclic aromatic hydrocarbons by manganese peroxidase of Nematoloma frowardii. FEMS Microbiol Lett 152:227-34. 96. Saparrat, M. C. N., Ana M. M. Bucsinszky, H. A. Tournier, M. N. Cabello and A. M. Arambarri. 2000. Extracellular ABTS-oxidizing activity of autochthonous fungal strains from Argentina in solid medium. Rev Iberoam Micol 17:64-68. 97. Smith, A. T., N. Santama, S. Dacey, M. Edwards, R. C. Bray, R. N. Thorneley, and J. F. Burke. 1990. Expression of a synthetic gene for horseradish peroxidase C in Escherichia coli and folding and activation of the recombinant enzyme with Ca2+ and heme. J Biol Chem 265:13335-43. 98. Smith, R. A., M. J. Duncan, and D. T. Moir. 1985. Heterologous protein secretion from yeast. Science 229:1219-24. 99. Sollewijn-Gelpke, M. D., M. Mayfield-Gambill, G. P. Lin-Cereghino, and M. H. Gold. 1999. Homologous expression of recombinant lignin peroxidase in Phanerochaete chrysosporium. Appl Environ Microbiol 65:1670-4. 100. Steffen, K. T., A. Hatakka, and M. Hofrichter. 2002. Degradation of humic acids by the litter-decomposing basidiomycete Collybia dryophila. Appl Environ Microbiol 68:3442-8. 101. Steffen, K. T., A. Hatakka, and M. Hofrichter. 2003. Degradation of benzo[a]pyrene by the litter-decomposing basidiomycete Stropharia coronilla: role of manganese peroxidase. Appl Environ Microbiol 69:3957-64. 102. Stephenson, J. N. 1950. Preparation & Treatment of Wood Pulp. McGraw-Hill Book company, Inc. 1st edition. 103. Stewart, P., and D. Cullen. 1999. Organization and differential regulation of a cluster of lignin peroxidase genes of Phanerochaete chrysosporium. J Bacteriol 181:3427-32. 104. Stewart, P., R. E. Whitwam, P. J. Kersten, D. Cullen, and M. Tien. 1996. Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. Appl Environ Microbiol 62:860-4. 105. Sigoillot, C., S. Camarero, T. Vidal, E. Record, M. Asther, M. Perez-Boada, M. J. Martinez, J. C. Sigoillot, M. Asther, J. F. Colom, and A. T. Martinez. 2005. Comparison of different fungal enzymes for bleaching high-quality paper pulps. J Biotechnol 115:333-43. 106. Sun, Y., and J. Cheng. 2002. Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1-11. 107. Sundaramoorthy, M., K. Kishi, M. H. Gold, and T. L. Poulos. 1994. The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-Å resolution. J Biol Chem 269:32759-67. 108. Takano, M., M. Nakamura, A. Nishida, and M. Ishihara. 2004. Manganese peroxidase from Phanerochaete crassa WD1694. Bulletin of FFPRI 3:7-13. 109. Tien, M., and T. K. Kirk. 1983. Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science 221:661-3. 110. Tien, M., and T. K. Kirk. 1984. Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proc Natl Acad Sci USA 81:2280-4. 111. Tien, M., T. K. Kirk, C. Bull, and J. Fee. 1986. Steady-state and transient-state kinetic studies on the oxidation of 3,4-Dimethoxybenzyl alcohol catalyzed by the ligninase of Phanerochaete chrysosporium Burds. J Biol Chem 261:1687-93. 112. Tien, M., and C. P. D. Tu. 1987. Cloning and sequencing of a cNA for a ligninase from Phanerochaete chrysosporium. Nature 326:520-3. 113. Timofeevski, S. L., G. Nie, N. S. Reading, and S. D. Aust. 1999. Addition of veratryl alcohol oxidase activity to manganese peroxidase by site-directed mutagenesis. Biochem Biophys Res Commun 256:500-4. 114. Timofeevski, S. L., N. S. Reading, and S. D. Aust. 1998. Mechanisms for protection against inactivation of manganese peroxidase by hydrogen peroxide. Arch Biochem Biophys 356:287-95. 115. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4. 116. Urzúa, U., P. J. Kersten, and R. Vicuña. 1998. Manganese peroxidase-dependent oxidation of glyoxylic and oxalic acids synthesized by Ceriporiopsis subvermispora produces extracellular hydrogen peroxide. Appl Environ Microbiol 64:68-73. 117. Wang, H., F. Lu, Y. Sun, and L. Du. 2004. Heterologous expression og lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica. Biotech Lett 26:1569-73. 118. Wang, Y., R. Vazquez-Duhalt, and M. A. Pickard. 2002. Purification, characterization, and chemical modification of manganese peroxidase from Bjerkandera adusta UAMH 8258. Curr Microbiol 45:77-87. 119. Whitwam, R., and M. Tien. 1996. Heterologous expression and reconstitution of fungal Mn peroxidase. Arch Biochem Biophys 333:439-46. 120. Whitwam, R. E., K. R. Brown, M. Musick, M. J. Natan, and M. Tien. 1997. Mutagenesis of the Mn2+-binding site of manganese peroxidase affects oxidation of Mn2+ by both compound I and compound II. Biochemistry 36:9766-73. 121. Whitwam, R. E., I. G. Gazarian, and M. Tien. 1995. Expression of fungal Mn peroxidase in E. coli and refolding to yield active enzyme. Biochem Biophys Res Commun 216:1013-7. 122. Wunderwald, U., G. Kreisel, M. Braun, M. Schulz, C. Jager, and M. Hofrichter. 2000. Formation and degradation of a synthetic humic acid derived from 3-fluorocatechol. Appl Microbiol Biotechnol 53:441-6. 123. Wuyep, P. A., A. U. Khan, and A. J. Nok. 2003. Production and regulation of lignin degrading enzymes from Lentinus squarrosulus (mont.) Singer and Psathyrella atroumbonata Pegler. African Journal of Biotechnology 2:444-447. 124. Yee, D. C., D. Jahng, and T. K. Wood. 1996. Enhanced expression and hydrogen peroxide dependence of lignin peroxidase from Streptomyces viridosporus T7A. Biotechnol. Prog 12:40-46. 125. Zhao, J., T. H. de Koker, and B. J. H. Janse. 1996. Comparative studies of lignin peroxidases and manganese-dependent peroxidases produced by selected white rot fungi in solid media. FEMS Microbiology Letters 145:393-399. 126. Zhao, J. D., and X. Q. Zhang. 1994. Importance, distribution and taxonomy of Ganodermataceae in China. Proceedings of Contributed Symposium 59A, B:3-5. 127. Ziegenhagen, D., and M. Hofrichter. 1998. Degradation of humic acids by manganese peroxidase from the white-rot fungus Clitocybula dusenii. J Basic Microbiol 38:289-99. 128. Zou, P., and H. Schrempf. 2000. The heme-independent manganese-peroxidase activity depends on the presence of the C-terminal domain within the Streptomyces reticuli catalase-peroxidase CpeB. Eur. J. Biochem. 267:2840-2849. 129. 呂鋒洲. 1994. 嘉南烏腳病患區景水中螢光腐植物質之研究:對於抗氧化作用酵素之影響. 行政院國家科學委員會專題研究計劃成果報告. 130. 呂鋒洲. 1995. 腐植酸及砷引起大白鼠末梢血管病變之研究. 行政院國家科學委員會專題研究計畫成果報告. 131. 卯曉嵐. 2000. 中國大型真菌. 河南科學技術出版社 1st edition. 132. 許瑞祥. 1990. 靈芝屬菌株鑑定系統之研究. 國立台灣大學農業化學研究所博士論文. 133. 張東柱. 1983. 台灣數種靈芝生物學上之研究. 台灣大學植物病蟲害學研究所碩士論文. 134. 王惠芳. 1996. 靈芝屬含錳超氧歧化酶基因之研究. 台灣大學農業化學研究所碩士論文. 135. 李時珍. 1976. 本草綱目. 國立中國醫藥研究所 3rd edition. 136. 傅昭憲. 1991. 台灣三種靈芝之培養性狀、病原性與腐朽之研究. 台灣大學森林學研究所碩士論文. 137. 施照輝. 2003. 白腐菌前處理對硫酸鹽紙漿漂白促進效果之研究. 國立臺灣大學森林學研究所博士論文. 138. 葉增勇. 1990. 台灣產南方靈芝複合種之分類學研究. 國立台灣大學植物學研究所博士論文. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24269 | - |
| dc.description.abstract | 靈芝屬(Ganoderma spp.)是一種白腐型真菌，具有各種可分解木材的外泌酵素，包括纖維素水解酵素(cellulase)和半纖維素水解酵素(hemicellulase),、果膠質水解酵素(pectinase)、漆氧化酶(laccase)、木質素過氧化酶(lignin peroxidase)和錳過氧化酶(manganese peroxidase)。其中錳過氧化酶是一種含有血基質(heme)的酵素，在二價錳離子以及過氧化氫存在的環境中能將酚類多環物質氧化分解，白腐型真菌利用此酵素分解木質素結構使其菌絲能有效深入木質纖維中分解纖維素等多醣以獲得養分。本實驗以含有ABTS的培養皿測試十株靈芝屬菌株的胞外酵素，發現所測試靈芝屬真菌都有可分解ABTS(2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid))的外泌酵素。並利用Genome Walking、五端RACE和PCR增幅技術自台灣靈芝(G. formosanum 0109, BZ)、新日本靈芝(G. neo-japonicum 0813, VZ)和南方靈芝(G. australe 0705)中選殖出六段新的錳過氧化酶基因序列。以RT-PCR增幅基因首尾端具有限制酶切位的台灣靈芝0109錳過氧化酶的cDNA，並將此基因轉殖入大腸桿菌(Escherichia coli)以及嗜甲醇畢赤氏酵母(Pichia methanolica)進行異源表達，得到在C端接有六個組胺酸(histidine)的錳過氧化酶以利後續純化的重組蛋白。 | zh_TW |
| dc.description.abstract | Lignin, component of plant secondary cell wall, binds to hemicellulose by covalent bonds and defends bacteria or fungi invading into the core of the wood. Lignin is a complicated polymer composed of aromatic compounds, which are ferulic acid, sinapic acid and p-coumaric acid etc. In paper pulping industry, lignin is hard-degradable and influences the brightness of paper. Generally, people use concentrated acid or alkali to precipitate or dissolve lignin. In recent study, the paper would be brighter and whiter by using manganese peroxidase in pulping procedure.
Manganese peroxidase, which was first isolated from white rot fungi Phanerochaete chrysosporium by Glenn and Gold in 1985, belongs to heme-containing peroxidase family. This extracellular enzyme is a glycoprotein with five disulfide bonds in general and contains two calcium ions in the structure. When it catalyses the substrates, hydrogen peroxide which produced by glyoxal oxidase or aryl-alcohol oxidase is necessary as electron donor. Mn(II) would be oxidized to Mn(III) by heme-oxygen radical complex. Mn(III) cations would be stabilized by alfa-hydroxyl acids, such as succinic acid or lactic acid, and oxidize lignin or lignin-like compounds. Manganese peroxidase has been found in many of white rot fungi, including Trametes versicolor, Pleurotus ostreatus, Armillaria mellea, etc, however, the studies of manganese peroxidase from Ganoderma spp. are few and most of them were focus on preotein level. The only published cDNA sequence was from G. applanatum. In this study, we cloned six new sequences of manganese peroxidase from G. formosanum, G. neo-japonicum and G. australe. Furthermore, we expressed the manganese peroxidase of G. formosanum in Escherichia coli and Pichia methanolica and produced recombinant enzyme. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:20:16Z (GMT). No. of bitstreams: 1 ntu-94-R92b47409-1.pdf: 1818604 bytes, checksum: fbd8e20e4248b3764a5942a579da33b1 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄
中文摘要……………………………………………..………………….V 英文摘要…………………………………………..…………...……….VI 表目錄…………………………………………..………………….......VII 圖目錄………………………………………..………...…………......VIII 第一章 前言…………………………………………………..…..……1 1.木質素………………………………………………………..…..…….1 1.1.木質素的來源及構造………………………………………..………1 1.2木質素在構型上的相似物…………………………………..………5 1.3木質素及其相似物對工業環境或生命之負面影響………..………5 1.4分解木質素的方法…………………………………………..…..…...7 2.錳過氧化酶…………………………………………………..…..…….7 2.1錳過氧化酶的研究歷史……………………………………..…..…...7 2.2與錳過氧化酶功能相似的酵素……………………………..…..…...9 2.3錳過氧化酶的特性及適用條件……………………………..……….9 2.4錳過氧化酶的基質和應用…………………………………..…..….14 2.5已開發之錳過氧化酶重組表現系統………………………..…..….18 2.5.1同源表達系統..………………………………………………..…..18 2.5.2異源表達系統..………………………………………………..…..19 2.5.2.1原核表達系統..………….…………………………………..…..19 2.5.2.2真核表達系統.…………….……………………..…………..….20 3.靈芝屬真菌..…………………………………………….………..…..22 3.1靈芝屬真菌的特性…………………………………….………..…..22 3.2靈芝屬真菌對植物的病害………………..…………….………..…24 3.3靈芝屬真菌木質分解酵素的研究………..…………….………..…24 4.異源表達系統……………………………..……………….……..…..26 4.1原核表達系統…………………………………………….……..…..26 4.2真核表達系統…………………………………………….……..…..26 4.2.1嗜甲醇畢赤氏酵母表達系統…………………….…….……..…..26 5.研究動機與目的…………………………………………….……..…27 第二章 材料與方法……………………………………….……..…...28 1.實驗材料…………………………………………………….……..…28 1.1實驗菌株………………………………………………….……..…..28 1.2實驗質體………………………………………………….……..…..30 1.3菌種保存………………………………………………….……..…..30 2.實驗流程及方法…………………………………………...……..…..30 2.1靈芝屬真菌ABTS分解能力測定..........………………...……..…..30 2.1.1培養皿測試…………………………………………......……..…..30 2.1.2胞外液酵素活性測試………………………………......……..….32 2.2錳過氧化酶基因選殖.........................................................................33 2.2.1真菌DNA製備……………………………………….......…..…..33 2.2.2錳過氧化酶片段聚合酶連鎖反應增幅………………......…..…..34 2.2.3 PCR產物純化及定序…………………………………….......…..34 2.2.4真菌RNA製備……….………………………………….......……34 2.2.5各菌株錳過氧化酶選殖方法…..…………….......………..…..….35 2.2.5.1台灣靈芝(G. formosanum 0109, BZ)錳過氧化酶基因選殖……35 2.2.5.1.1 Rapid Genome Walking……………………………………….35 2.2.5.1.2片段cDNA合成………………………………………...……36 2.2.5.1.3五端RACE ( Rapid Amplification of cDNA Ends)……..……36 2.2.5.1.4 台灣靈芝(G. formosanum BZ)錳過氧化酶基因選殖……….37 2.2.5.2 南方靈芝(G. australe 0705)錳過氧化酶基因選殖……………37 2.2.5.3新日本靈芝(G. neo-japonicum 0813, VZ)錳過氧化酶基因選殖………………………………………………………………………..37 2.2.6胺基酸序列分析…………………………………………………..38 2.3錳過氧化酶基因之異源表達系統建構及表達......……..………….42 2.3.1.1大腸桿菌表達系統建構………………….......….…..………….42 2.3.1.2以大腸桿菌表達錳過氧化酶…………….......…...…………….42 2.3.2.1嗜甲醇畢赤氏酵母表達系統建構……….....….…..…..……….43 2.3.2.2以嗜甲醇畢赤氏酵母表達錳過氧化酶….......…...…..…..…….43 2.3.2.2.1培養皿測試………………………..…….......……..………….44 2.3.3蛋白質分析……………………………….......……..……...…….44 2.3.3.1活性測試……………………………..........……..………..…….44 2.3.3.2 蛋白質定量…………….………………………………………44 2.3.3.3十二烷基磺酸鈉聚丙烯醯胺膠體電泳...…...…….……...…….44 2.3.3.4.西方雜合分析………………………….….......……..…...…….45 2.3.3.5重組蛋白之純化……………………………...…………………45 第三章 結果…………………………………….......……..…....…….46 1.靈芝屬真菌分解ABTS能力之初步測定………….......……....…….46 1.1培養皿測試………………………….......……..…...……………….46 1.2 菌絲體胞外酵素活性測定………….......……..…......……………50 2. 靈芝屬真菌錳過氧化酶基因選殖…….......……..…...…….………50 2.1 錳過氧化酶基因片段選殖………….......……..…...……...………51 2.2 錳過氧化酶轉譯區間選殖………….......……..…...……...………51 2.2.1 台灣靈芝0109基因選殖…………………...……………………51 2.2.1.1Genome Walking…………..…………….………………………51 2.2.1.2 RT-PCR和五端RACE…..…………….………………………..54 2.2.1.3台灣靈芝BZ錳過氧化酶基因選殖………….…………………54 2.3.2 南方靈芝0705錳過氧化酶基因選殖…………….…..…………57 2.3.3 新日本靈芝0813, VZ錳過氧化酶基因選殖….…...……………57 2.3.4 錳過氧化酶片段基因分析………….………………..…….……60 2.3.5 南方靈芝0705和台灣靈芝0109錳過氧化酶轉譯區間比較..…60 2.3.6 靈芝屬物種錳過氧化酶胺基酸序列和其他物種之比較…....…69 3.靈芝錳過氧化酶的異源表達………….………………..……..……..72 3.1大腸桿菌表達系統………….………………..……..…………..…..72 3.1.1表達載體建構………….………………..……..…………..……...72 3.1.2 目標蛋白表達………….………………..……..…………..…….72 3.2嗜甲醇畢赤氏酵母菌表達系統……………..……..……………….77 3.2.1表達載體建構………….………………..……..…………..……...77 3.2.2目標蛋白表達………….………………..……..…………..……...77 第四章 綜合討論與結果…………..……..……….…………..……...80 第五章 未來展望……………………………………………………..84 參考文獻………….……………………..……..………………..……...85 | |
| dc.language.iso | zh-TW | |
| dc.title | 靈芝屬菌株錳過氧化酶的選殖與表達 | zh_TW |
| dc.title | Cloning and Expression of Manganese Peroxidase Genes from Ganoderma spp. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 趙維良(Wei-Liang Chao),黃慶璨(Ching-Tsan Huang) | |
| dc.subject.keyword | 錳過氧化酶,靈芝,白腐型真菌,畢赤氏酵母,木質素, | zh_TW |
| dc.subject.keyword | Manganese peroxidase,MnP,Ganoderma,White rot fungi,Pichia methanolica,lignin, | en |
| dc.relation.page | 95 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2005-07-28 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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