請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24241完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 常玉強(Yuh-Chyang Charng) | |
| dc.contributor.author | Kuan-Te Li | en |
| dc.contributor.author | 李冠德 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:19:27Z | - |
| dc.date.copyright | 2005-07-30 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-28 | |
| dc.identifier.citation | Aarts, M. G., W. G. Dirkse, W. J. Stiekema and A. Pereira (1993). Transposon tagging of a male sterility gene in Arabidopsis. Nature 363 : 715-717.
Bradshaw LD, Padgette SR, Kimball SL, Wells BH (1997) Perspectives on glyphosate resistance. Weed Technol. 11: 189–198. Balcells, L., E. Sundberg and G. Coupland (1994). A heat-shock promoter fusion to the Ac transposase gene drives inducible transposition of a Ds element during Arabidopsis embryo development. Plant J. 5: 755-764. Baerson SR, Rodriguez DJ, Tran M, Feng Y, Biest NA, Dill GM (2002) Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:1265-75. Charng, Y.-C., A. J. P. Pfitzner, U. M. Pfitzner, K.-F. Charng-Chang, C.-M. Chen, Jenn Tu and T.-T. Kuo (2000). Construction of an inducible transposon, INAc, to develop a gene tagging system in higher plants. Mol. Breed. 6: 353-367. Charng, Y.C. & Hsieh, C.S. (2004) Construction of an inducible transposon with promoterless reporter gene. Crop, Environment and Bioinformatics 1: 89-101. Chen, L., Marmey, P., Taylor, N.J., Brizard, J.-P., Espinoza, C., D’Cruz, P., Huet, H., Zhang, S., de Kochko, A., Beachy, R.N., Fauquet, C.M., (1998). Expression and inheritance of multiple transgens in rice plants. Nat. Biotechnol. 16:1060–1064. Cobb AH, Kirkwood RC (2000) Herbicides and their Mechanisms of Action. CRC Press, Boca Raton, FL. Cotsaftis, O., Sallaud, C., Breitler, J.C., Meynard, D., Greco, R., Pereira, A., Guiderdoni, E., (2002). Transposon-mediated generation of T-DNA- and marker-free rice plants expressing a Bt endotoxin gene. Mol. Breed. 10:165–180. Ebinuma, H., Sugita, K., Matsunaga, E., Yamakado, M., (1997). Selection of marker-free transgenic plants using the isopentyl transferase gene. Proc. Natl. Acad. Sci. U.S.A. 94, 2117–2121. Enoki, H., T. Izawa, M. Kawahara, M. Komatsu, S. Koh, J. Kyozuka and K. Shimamoto (1999). Ac as a tool for the functional genomics of rice. Plant J. 19: 605-613. Firek, S., D. J. Martin, M. R. Roberts, F. Sturgess, R. Scott and J. Draper (1996). Gametophyte-specific transposition of the maize Ds element in transgenic tobacco. Plant J. 10:569-578. Greco, R., P. B. Ouwerkerk, A. J. Taal, C. Favalli, T. Beguiristain, P. Puigdomenech, L. Colombo, J. H. Hoge and A. Pereira (2001). Early and multiple Ac transpositions in rice suitable for efficient insertional mutagenesis. Plant Mol. Biol. 46: 215-227. Greco, R., P. B. F. Ouwerkerk, R. J. d. Kam, C. Sallaud, C. Favalli, L. Colombo, E. Guiderdoni, A. H. Meijer, J. H. C. Hoge and A. Pereira (2003). Transpositional behaviour of an Ac/Ds system for reverse genetics in rice. Theor. Appl. Genet. 108: 10-24. Hajdukiewicz, P.T., (2001). Multiple pathways for Cre/lox-mediated recombination in plastids. Plant J. 27:161–170. Haring, M. A., C. M. Rommens, H. J. Nijkamp and J. Hille (1991). The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies. Plant Mol. Biol. 16: 449-461. Howe Arlene R., Charles S. Gasser, Sherri M. Brown, Stephen R. Padgette, Jesse Hart, Gregory B. Parker, Michael E. Fromm, Charles L. Armstrong (2002) Glyphosate as a selective agent for the production of fertile transgenic maize (Zea mays L.) plants. Mol. Breed. 10:153-164. Hu T., S. Metz, C. Chay, H. P. Zhou, N. Biest, G. Chen, M. Cheng, X. Feng, M. Radionenko, F. Lu, J. Fry (2003) Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection. Plant Cell Reports 21:1010-1019. Jones, D. A., C. M. Thomas, K. E. Hammond-Kosack, P. J. Balint-Kurti and J. D. Jones (1994). Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science 266: 789-793. Kohli, A., J. Xiong, R. Greco, P. Christou and A. Pereira (2001). Tagged Transcriptome Display (TTD) in indica rice using Ac transposition. Mol. Genet. Genomics 266: 1-11. Liu, Y.-G., N. Mitsukawa, T. Oosumi and R. F. Whittier (1995). Efficient isolationand mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J. 8: 457-463. Matthews, P.R., Waterhouse, P.M., Thornton, S., Fieg, S.J., Gubler, F., Jacobsen, J.V., (2001). Marker gene elimination from transgenic barley, using co-transformation with adjacent ‘twin T-DNAs’ on a standard Agrobacterium transformation vector. Mol. Breed. 7：195–202. McCormac, A. C., Elliott, M.C., and Chen, D.F.(1998) A simple method for the production of highly competent cells of Agrobacterium for transformation via electroporation. Mol. Biotechnol. 9:155-159. Miki, B. and S. McHugh (2004). 'Selectable marker genes in transgenic plants: applications, alternatives and biosafety.' J. of Biotechnology 107(3): 193-232. Miller, M., Tagliani, L., Wang, N., Berka, B., Bidney, D., Zhao, Z.Y., (2002). High efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumifaciens 2 T-DNA binary system. Trans. Res. 11:381–396. Nakagawa, Y., C. Machida, Y. Machida and K. Toriyama (2000). Frequency and pattern of transposition of the maize transposable element Ds in transgenic rice plants. Plant Cell Physiol. 41: 733-742. Nishal, B., T. Tantikanjana and V. Sundaresean (2005). An Inducible Targeted Tagging System for Localized Saturation Mutagenesis in Arabidopsis. Plant Physiol. 137:3-12. Ochman, H., A. S. Gerber and D. L. Hartl (1988). Genetic applications of an inverse polymerase chain reaction. Genetics 120: 621-623. Ow, D.W., (2001). The right chemistry for marker gene removal? Nat. Biotechnol. 19:115–116. Padgette SR, Re DB, Gasser CS, Eichholtz DA, Frazier RB, Hironaka CM, Levine EB, Shah DM, Fraley RT, Kishore GM (1991) Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site. J. Biol. Chem. 266: 22364–22369. Padgette SR, Re DB, Barry GF, Eichholtz DE, Delannay X, Fuchs RL, Kishore GM, Fraley RT (1996) New weed control opportunities: development of soybeans with a Roundup Ready gene. In S Duke, ed, Herbicide Resistant Crops: Agricultural, Economic, Environmental, Regulatory, and Technological Aspects. CRC Press, Boca Raton, FL, pp53–84. Sambrook, J., Fritsch E. F., and Maniatis T. (1989) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Schönbrunn E, Eschenburg S, Shuttleworth WA, Schloss JV, Amrhein N, Evans JNS, Kabsch W (2001) Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proc Natl Acad Sci USA 98: 1376–1380. Sha, Y., S. Li, Z. Pei, L. Luo, Y. Tian and C. He (2004). Generation and flanking sequence analysis of a rice T-DNA tagged population. Theor. Appl. Genet. 108:306-314. Sheu, J. J., Yu T. S., and Yu S. M. (1996) Carbohydrate starvation stimulates differential expression of rice alpha-amylase genes that is modulates through complicated transcriptional and posttranscriptional processes. J. Biol. Chem. 271:26998-7004. Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-17. Tissier, A. F., S. Marillonnet, V. Klimyuk, K. Patel, M. A. Torres, G. Murphy and J. D. Jones (1999). Multiple independent defective suppressor-mutator transposon insertions in Arabidopsis: a tool for functional genomics. Plant Cell 11: 1841-1852. Triglia, T., M. G. Peterson and D. J. Kemp (1988). A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16: 186. Upadhyaya, N. M., X.-R. Zhou, Q.-H. Zhu, K. Ramm, L. Wu, A. Eamens, R. Sivakumar, T. Kato, D.-W. Yun, C. Santhoshkumar, K. K. Narayanan, J. W. Peacock and E. S. Dennis (2002). An iAc/Ds gene and enhancer trapping system for insertional mutagenesis in rice. Funct. Plant Biol. 29: 547-559. Whitham, S., S. P. Dinesh-Kumar, D. Choi, R. Hehl, C. Corr and B. Baker (1994). The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor. Cell. 78: 1101-1115. Wu L, Nagano H, Yoshida K, Kawasaki S, Kishima Y, Sano Y (2001) Mapping of the sequences expressed in the rice callus onto a BAC contig: identification of a genomic segment carrying closely linked genes encoding EPSP synthase and rps20. Unpublished. Yan, Y., C. An, L. Li, J. Gu, G. Tan and Z. Chen (2003). T-linker-speciAc ligation PCR (T-linker PCR): an advanced PCR technique for chromosome walking or for isolation of tagged DNA ends. Nucleic Acids Res. 31(12): e68, 7 pp. Ye, G.-N., S. M. Colburn, et al. (2003). Persistence of Unselected Transgenic DNA during a Plastid Transformation and Segregation Approach to Herbicide Resistance. Plant Physiol. 133: 402-410. Yuan CI, Chiang MY, Chen YM (2002) Triple mechanisms of glyphosate-resistance in a naturally occurring glyphosate-resistant plant Dicliptera chinensis. Plant Sci. 163: 543-554. Zubco, E., Scutt, C., Meyer, P., (2000). Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat. Biotechnol. 18:442–445. Zuo, J., Niu, Q.-W., Moller, S.G., Chua, N.-H., (2001). Chemical-regulated, site-specific DNA excision in transgenic plants. Nat. Biotechnol. 19:157–161. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24241 | - |
| dc.description.abstract | 轉位子可分為兩部分：轉位酶基因及受轉位酶作用之兩端點。本研究之目的及原理為將可誘導轉位子的端點構築在目標基因的隱子（intron）中，目標基因經由剪接作用（splicing），插入隱子的端點不影響基因正常表現；當目標基因表現後，以誘導劑驅動可誘導轉位子表現，使其轉位至染色體的其他位置，帶走部份目標基因，使該基因不完整，以達到移除目標基因的目的。
本研究實例以EPSPS ( 5-enolpyruvylshikimate-3-phosphate synthase )作為轉基因篩選標記基因，水稻內生EPSPS基因經二點突變（將胺基酸序列位置168之Glycine突變為Alanine；位置211之Glycine突變為Aspartic acid），使其由對嘉磷塞（glyphosate）敏感型轉為抵抗型，而成為篩選標記基因。將 Ac 轉位子的 5’端插入修飾之 EPSPS 基因的第一個隱子，並以 CaMV 35S promoter作為啟動子；另將PR-1a啟動子（pathogenesis-related protein 1a, 可受水楊酸誘導表現）與 Ac 轉位酶融合，構築於 Ac的兩端點之間。完成上述構築後，以農桿菌法將此構築轉殖至水稻癒傷組織，於添加嘉磷塞之篩選培養基，在野生型癒傷組織的致死濃度下（5 mM），篩選出轉殖系後，證明修飾之EPSPS基因可作為篩選標記。其後，將癒傷組織移至含誘導劑水楊酸的培養基，誘導轉位，在T0世代的癒傷組織中，經 PCR 分析，的確觀察有轉位事件（transposition event），即包含轉位子本身，CaMV 35S 啟動子與修飾之 EPSPS 基因的第一個顯子（exon）同時隨之轉位，修飾之 EPSPS 基因的基因序列不再完整。據此，癒傷組織發育成完整植株後，可在後代取得完全轉位的轉殖株，達到篩選標記基因移除的目的。 | zh_TW |
| dc.description.abstract | The maize transposable element Ac can move to new location within genome. In this work, an Ac–based inducible transposon was constructed to develop a one-time gene expression system. Firstly, the 5’ end of the Ac was inserted in the first intron of the modified rice EPSPS gene, which triggered by the CaMV35S promoter. The inducible transposon was completed by locating the PR-1a promoter-transposase fusion and the CaMV35S promoter was between the 5’ and 3’ ends. Thus, this inducible transposon contains not only the PR-TPase fusion but also the first exon of the modified EPSPS gene and its promoter. This construct, which termed as KCEH, was introduced into rice. The modified EPSPS gene can be transcribed normally and allow the transgenic rice to be Glyphosate-resistant. After the Glyphosate screening, the transgenic rice plants were treated with salicylic acid for the excision of the inducible transposon. As a result of this, the modified EPSPS gene was truncated by losing its first exon and the 35S promoter. Emphasis has been placed on the theoretical and practical aspects of the approach that may be relevant to its application to other gene expression regulatory system. It provides a means to investigate a one-time gene expression system. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:19:27Z (GMT). No. of bitstreams: 1 ntu-94-R92621109-1.pdf: 3667234 bytes, checksum: 03c6825d3c753d014d597eaf9661f829 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄……………………………………………………………………………I
圖表目錄、附錄目錄…………………………………………………………II 中文摘要………………………………………………………………………1 英文摘要………………………………………………………………………2 前言……………………………………………………………………………3 前人研究………………………………………………………………………4 材料與方法………………………………………………………………….12 結果………………………………………………………………………….30 討論………………………………………………………………………….37 未來展望…………………………………………………………………….42 引用文獻…………………………………………………………………….43 | |
| dc.language.iso | zh-TW | |
| dc.subject | 水稻 | zh_TW |
| dc.subject | 篩選標記基因去除 | zh_TW |
| dc.subject | 篩選標記基因 | zh_TW |
| dc.subject | 嘉磷塞 | zh_TW |
| dc.subject | 可誘導轉位子 | zh_TW |
| dc.subject | 5-enolpyruvylshikimate-3-phosphate synthase | en |
| dc.subject | selection marker | en |
| dc.subject | inducible transposon | en |
| dc.subject | marker-free | en |
| dc.subject | glyphosate | en |
| dc.subject | rice | en |
| dc.title | 可誘導轉位子單次移除篩選標記基因之研究 | zh_TW |
| dc.title | Investigation of inducible transposon to remove selection marker | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 杜鎮(Jenn Tu),林納生(Na-Sheng Lin),黃鵬林(Pung-Ling Huang),盧虎生(Huu-Sheng Lur) | |
| dc.subject.keyword | 篩選標記基因,可誘導轉位子,篩選標記基因去除,嘉磷塞,水稻, | zh_TW |
| dc.subject.keyword | selection marker,inducible transposon,marker-free,glyphosate,rice,5-enolpyruvylshikimate-3-phosphate synthase, | en |
| dc.relation.page | 70 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2005-07-28 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-94-1.pdf 未授權公開取用 | 3.58 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。