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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 費昌勇 | |
| dc.contributor.author | Chia-Ju Chung | en |
| dc.contributor.author | 鍾佳儒 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:15:59Z | - |
| dc.date.copyright | 2006-02-09 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-02-03 | |
| dc.identifier.citation | 1. Andrasko, J., and B. Rosen. 1994. Sensitive identification of hemoglobin in bloodstains from different species by high performance liquid chromatography with combined UV and fluorescence detection. J Forensic Sci 39:1018-25.
2. Aranishi, F. 2005. PCR-RFLP analysis of nuclear nontranscribed spacer for mackerel species identification. J Agric Food Chem 53:508-11. 3. Bataille, M., K. Crainic, M. Leterreux, M. Durigon, and P. de Mazancourt. 1999. Multiplex amplification of mitochondrial DNA for human and species identification in forensic evaluation. Forensic Sci Int 99:165-70. 4. Bell, J. L., and J. P. Grassle. 1998. A DNA probe for identification of larvae of the commercial surfclam (Spisula solidissima). Mol Mar Biol Biotechnol 7:127-37. 5. Bellagamba, F., V. M. Moretti, S. Comincini, and F. Valfre. 2001. Identification of species in animal feedstuffs by polymerase chain reaction-restriction fragment length polymorphism analysis of mitochondrial DNA. J Agric Food Chem 49:3775-81. 6. Bellis, C., K. J. Ashton, L. Freney, B. Blair, and L. R. Griffiths. 2003. A molecular genetic approach for forensic animal species identification. Forensic Sci Int 134:99-108. 7. Branicki, W., T. Kupiec, and R. Pawlowski. 2003. Validation of cytochrome b sequence analysis as a method of species identification. J Forensic Sci 48:83-7. 8. Bravi, C. M., J. P. Liron, P. M. Mirol, M. V. Ripoli, P. Peral-Garcia, and G. Giovambattista. 2004. A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene. Leg Med (Tokyo) 6:246-51. 9. Buntjer, J. B., J. A. Lenstra, and N. Haagsma. 1995. Rapid species identification in meat by using satellite DNA probes. Z Lebensm Unters Forsch 201:577-82. 10. Calvo, J. H., P. Zaragoza, and R. Osta. 2001. Random amplified polymorphic DNA fingerprints for identification of species in poultry pate. Poult Sci 80:522-4. 11. Cota-Rivas, M., and B. V. Vallejo-Cordoba. 1997. Capillary electrophoresis for meat species differentiation. J Capillary Electrophor 4:195-9. 12. Dooley, J. J., H. D. Sage, M. A. Clarke, H. M. Brown, and S. D. Garrett. 2005. Fish species identification using PCR-RFLP analysis and lab-on-a-chip capillary electrophoresis: application to detect white fish species in food products and an interlaboratory study. J Agric Food Chem 53:3348-57. 13. Du, S. J., Shih,Y.C. 1993. Development of cooked meat species identification. Proceedings of the 1993 fiscal year symposium on food hygiene and analysis science, pp 43-54. 14. Ellis, D. I., D. Broadhurst, S. J. Clarke, and R. Goodacre. 2005. Rapid identification of closely related muscle foods by vibrational spectroscopy and machine learning. Analyst 130:1648-54. 15. Fugate, H. G., Jr., and S. R. Penn. 1971. Immunodiffusion technique for the identification of animal species. J Assoc Off Anal Chem 54:1152-6. 16. Hoyem, T., and B. Thorson. 1970. Myoglobin electrophoretic patterns in identification of meat from different animal species. J Agric Food Chem 18:737-9. 17. Hsieh, H. M., H. L. Chiang, L. C. Tsai, S. Y. Lai, N. E. Huang, A. Linacre, and J. C. Lee. 2001. Cytochrome b gene for species identification of the conservation animals. Forensic Sci Int 122:7-18. 18. Hsieh, H. M., L. H. Huang, L. C. Tsai, Y. C. Kuo, H. H. Meng, A. Linacre, and J. C. Lee. 2003. Species identification of rhinoceros horns using the cytochrome b gene. Forensic Sci Int 136:1-11. 19. Krcmar, P., and E. Rencova. 2001. Identification of bovine-specific DNA in feedstuffs. J Food Prot 64:117-9. 20. Krcmar, P., and E. Rencova. 2003. Identification of species-specific DNA in feedstuffs. J Agric Food Chem 51:7655-8. 21. Krzynowek, J., and K. Wiggin. 1979. Identification of species in cooked crabmeat by thin layer isoelectric focusing. J Assoc Off Anal Chem 62:630-6. 22. Lee, J. C., and J. G. Chang. 1994. Random amplified polymorphic DNA polymerase chain reaction (RAPD PCR) fingerprints in forensic species identification. Forensic Sci Int 67:103-7. 23. Lundstrom, R. C. 1981. Fish species identification by isoelectric focusing: sarcoplasmic protein polymorphism in monkfish (Lophius americanus). J Assoc Off Anal Chem 64:32-7. 24. Mackie, I. M. 1968. Species identification of cooked fish by disc electrophoresis. Analyst 93:458-60. 25. Naito, E Dewa, K Yamanouchi, H Takagi, S and Kominami R, 1993. Sex determination using the hypomethylation of a human macro-satellite DXZ4 in female cells.Nucleic Acids Res. 21: 2533–34. 26. Novick, G.E. Novick, C.C. Yunis, J. Yunis, E. Martinez, K. Duncan, G.G. Troup, G.M. Deininger, P.L. Stoneking, M. Batzer, M.A. et al., 1995. Polymorphic human specificity Alu insertions as markers for human identification, Electrophoresis 16: 1596–1601. 27. Parson, W., K. Pegoraro, H. Niederstatter, M. Foger, and M. Steinlechner. 2000. Species identification by means of the cytochrome b gene. Int J Legal Med 114:23-8. 28. Rehbein, H. 1992. Fish species identification by peptide mapping of the myosin heavy chain. Electrophoresis 13:805-6. 29. Shaw, F. D., E. M. Deane, and D. W. Cooper. 1983. An immunodiffusion method for the identification of the species of origin of meat samples. Aust Vet J 60:25. 30. Sinclair, A. J., and W. J. Slattery. 1982. Identification of meat according to species by isoelectric focusing. Aust Vet J 58:79-80. 31. Slattery, W. J., and A. J. Sinclair. 1983. Differentiation of meat according to species by the electrophoretic separation of muscle lactate dehydrogenase and esterase isoenzymes and isoelectric focusing of soluble muscle proteins. Aust Vet J 60:47-51. 32. Swart, K. S., and C. R. Wilks. 1982. An immunodiffusion method for the identification of the species of origin of meat samples. Aust Vet J 59:21-2. 33. Terol, J., R. Mascarell, V. Fernandez-Pedrosa, and M. Perez-Alonso. 2002. Statistical validation of the identification of tuna species: bootstrap analysis of mitochondrial DNA sequences. J Agric Food Chem 50:963-9. 34. Trotta, M., S. Schonhuth, T. Pepe, M. L. Cortesi, A. Puyet, and J. M. Bautista. 2005. Multiplex PCR method for use in real-time PCR for identification of fish fillets from grouper (Epinephelus and Mycteroperca species) and common substitute species. J Agric Food Chem 53:2039-45. 35. Ukishima, Y., M. Kino, H. Kubota, S. Wada, and S. Okada. 1991. Identification of whale species by thin-layer isoelectric focusing of sarcoplasmic proteins. J Assoc Off Anal Chem 74:943-50. 36. . Wan, Q. H., and S. G. Fang. 2003. Application of species-specific polymerase chain reaction in the forensic identification of tiger species. Forensic Sci Int 131:75-8. 37. Waye, J.S. Presley, L.A. Budowle, B. Shutler, G.G. Fourney, R.M. 1989. A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts, Biotechniques 7: 852–855. 38. Zehner, R., S. Zimmermann, and D. Mebs. 1998. RFLP and sequence analysis of the cytochrome b gene of selected animals and man: methodology and forensic application. Int J Legal Med 111:323-7. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24103 | - |
| dc.description.abstract | 在許多東亞社會中，因為肉用、毛皮或者為其他需要，許多狗貓被不人道地任意屠宰。隨著台灣動物保護法的實施，肉品之物種鑑定技術便成為行政機關對不法業者處罰時的重要依據。此外，有一些有害外來物種如美洲巨水鼠(Myocastor coypus)遠赴重洋地從原棲地經由走私入侵台灣，並且可能逐漸對台灣溼地生態系統造成傷害。對於此類有害外來種生物的鑑定結果，亦為其非法進口、飼養及販賣之違法行為之重大證據。本論文係以增幅粒線體DNA上之cytochrome b基因片段來作為鑑定方法，對一些品質不良的檢體，例如腐壞或者過度烹煮的肉或者毛皮仍然適用，在整個鑑定過程中，每個檢體只需要萃取出15 ng的DNA便足以辨識。本鑑定法係利用三對專一性的引子將各標的物種cytochrome b基因片段以是否分別增幅出252、292及297 bp大小的片段來判斷為標的物種與否。本實驗已將所得PCR產物的定序結果及與已在EMBL DNA資料庫上發表的序列進行比對，結果均確實無誤，以確保各鑑定結果的正確性。 | zh_TW |
| dc.description.abstract | Many dogs and cats are slaughtered in East-Asia because of the needs in their meat for food or other purposes. Identification of the species is required to determine that a crime against Animal Protection Law in Taiwan has been committed. Otherwise, some harmful species like nutria (Myocastor coypus) has invaded Taiwan far from the place they originally inhabit, and may damage the ecological system of wetland in Taiwan gradually. This thesis details a good DNA technique using part of the cytochrome b gene on the mitochondrial genome that can work on poor-quality samples such as decayed or over-cooked meat. An appropriate deoxyribonucleic acid (DNA) extraction technique is required to obtain at least 15 ng of DNA. Amplification of part of the cytochrome b gene using specific primers produces a fragment of 252/292/297 bp in size. Direct sequencing of the PCR products allows confirmation of the DNA sequences to those already described on the GENbank-EMBL DNA database to ensure the results of identification. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:15:59Z (GMT). No. of bitstreams: 1 ntu-95-R91629016-1.pdf: 1310483 bytes, checksum: 10af2df295c23bb0cd62197e9a188057 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | Table of contents……………………………………………………I
List of illustration…………………………………………………………II Abstract in Chinese ……………………………………………III Abstract in English ………………………………………………IV 1. Introduction 1.1 Significances of identification of these meat species like domestic dogs, domestic cats and nutria………………………………………………..1 1.2 Identification of animals whose keep, export, or import is banned in Taiwan (Myocastor coypus) …….……………………………………………...2 1.3 Identification of canine and feline flesh for Animal Protection Law enforcement in Taiwan……………..………………………………......4 2. Literature Reviews 2.1 History of identification of meat species………………………………7 2.2 Properties mitochondrial DNA ............………………………………..9 2.3 Application of cytochrome b gene amplification by PCR……………10 3. Materials and Methods 3.1 Experiments designation and samples collection…….………………11 3.2 Deparaffin, DNA extraction and purification………………………...13 3.3 PCR amplification, sensitivity and specificity test…………………...19 3.4 Gel electrophoresis and retrieve of PCR products from gel.....……....23 3.5 Sequencing and sequences computation with GCG………………….27 4. Results 4.1 Experiments designation and samples collection…………………….28 4.2 PCR amplification, sensitivity and specificity test.………………….34 4.3 Sequencing and sequences computation with GCG………………….39 5.Discussion………………………………………………………43 6.Conclusion and Future works…………………………………47 7.Reference…………………………………………………………48 8.Appendix…………………………………………………………52 | |
| dc.language.iso | en | |
| dc.subject | 種別鑑定 | zh_TW |
| dc.subject | 狗肉 | zh_TW |
| dc.subject | 貓肉 | zh_TW |
| dc.subject | 美洲巨水鼠 | zh_TW |
| dc.subject | 肉品 | zh_TW |
| dc.subject | identification | en |
| dc.subject | nutria | en |
| dc.subject | cat meat | en |
| dc.subject | dog meat | en |
| dc.title | 以增幅細胞色素b基因片段鑑定家犬、家貓及美洲巨水鼠來源肉品 | zh_TW |
| dc.title | Identification of Meat Species by Amplifying Partial Cytochrome b Gene Fragments for Domestic Dogs, Domestic Cats and Nutria | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許桂森,廖震元 | |
| dc.subject.keyword | 狗肉,貓肉,美洲巨水鼠,肉品,種別鑑定, | zh_TW |
| dc.subject.keyword | dog meat,cat meat,nutria,identification, | en |
| dc.relation.page | 65 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2006-02-04 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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