以有機光電半導體微型化表面電漿共振檢測系統

Abstract

近年來由於許多傳染病的出現，造成全球大恐慌以及死亡人數激增。傳染性疾病的檢驗，最主要就是確定病原體的種類。病原體是指引起疾病的微小生物，而確定病原體種類之後才能對症下藥。目前疾病的檢測法分為基因檢測與蛋白質檢測兩大類技術，應用於體外檢測的產品市場已近300億美元，並近年預估可以7％的幅度繼續攀升，仍具有相當大的發展空間。 在本研究中，應用高分子有機光電半導體材料與微機電製程技術製作100μm線寬的PLED平面式環型光源及感測金膜，依晶片與元件尺寸特性直接做光學對準，具有0.25°的準確度，並進行定角度反射光強度監測以達成生物分子的檢定。將原本使用大型儀器與電腦分析之表面電漿共振系統簡化為一4x5cm2晶片，並具有使不需額外進行檢體標誌的表面電漿共振檢測技術具有向戶外即時檢測發展的可能性，同時在檢測中也可減少光路對準的步驟，並省去稜鏡、濾光片及極化片多數光學器材的使用，成為一種非常簡便之即時檢測晶片。此外，再進行微流道系統之整合，可減低樣本需求，並發展平行檢測之技術。未來此技術可朝疾病檢驗、藥物篩選為主要發展目標。

In recent years, the appearance of many epidemics have risen a disturbance and increased the casualties in the whole world. In epidemic diagnosis, the most important is to identify the pathogen which is a tiny organism to cause a disease. To prescribe medicine, it must be confirm with the pathogen. The methods of disease diagnosis classified into gene diagnosis and protein diagnosis at present The market of the in vitro diagnostic (IVD) technologies is approximately 30 billion U.S. dollars, and will see an annual average growth of 7% for the near future. There are still plenty room for IVD advancement. In this research, we apply the polymer light-emitting diode and the surface micro-machining technology into the field of surface plasmon resonance (SPR), to construct a planar circular light source and a sensing gold film both 100um in width which produce SPR signal by aiming with the dimensional characterization in a resolution about 0.25 degree . Such that we can operate a fixed-angle SPR test with bio-molecule. The miniaturized SPR system has an opportunity for non-labeling outdoor sensing. The sensing chip is convenient in examination by saving not only the manpower of aiming optical pathway but the usage of lots optical equipments, prism, filter, and polarizer. Furthermore, it can lower the sample requirement and develop parallel diagnosis method by integrating the chip with the micro-fluidic channel system. This lab-on-a-chip system can be utilized in disease diagnosis and drug screen in the future.