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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳炳煇(Ping-Hei Chen) | |
| dc.contributor.author | Cheng-Hao Yang | en |
| dc.contributor.author | 楊証皓 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:09:52Z | - |
| dc.date.copyright | 2011-08-22 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-20 | |
| dc.identifier.citation | References
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23768 | - |
| dc.description.abstract | A polymeric DNA sensor using radio frequency detection was developed in this thesis. This biosensor presents an electrical DNA detection method for a coplanar wave guide structures fabricated on a multi-layer polymer. Gold nanoparticles and magnetic nanoparticles are employed to immobilize on the surface of the biosensor to enhance overall detection sensitivity. The surface of the proposed biochip used thioglycolic acid (TGA) chemical reagent to form thiolated modified sensing surface for DNA hybridization. Since the electromagnetic characteristic of the biosensors can be altered through the establishment of multilayer nanoparticles, the performance of the proposed biosensor can be evaluated by the shift of the center frequency of the RF biosensor.
The shift of the center frequency is increasing with the raise of the target DNA concentrations. Among the experimental results, the detection limit of the developed biosensor for the DNA detection is 10pM. Moreover, the measured center frequency of the biosensor without nanoparticles is about 6.4 GHz, which is close to the simulated results. The shifts of center frequency using AuNPs and MNPs triple-layer are about 0.9 and 0.7 GHZ, which exceed the shift of the double-layer AuNPs and MNPs by 0.6 and 0.5 GHz, respectively. The polymer biosensor for DNA detection using nano-electromechanical system (NEMS), Radio Frequency (RF), surface modification, and biochemical detection technology is successfully completed. The polymer materials are utilized as substrates to replace conventional substrate such as glass and silicon wafers. Therefore, the price of each biochip is effectively educed by using fully polymeric materials. In this study, the developed biosensor can attain inexpensive, disposable, and high-sensitive advantages for biomedicine diagnostic system. As biotechnology becomes more and more indispensable nowadays, the developed biosensor has potential applications for precise recognizing, fast sensing, and reliable quantifying in disease diagnosis and health care. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:09:52Z (GMT). No. of bitstreams: 1 ntu-100-D95522002-1.pdf: 5707531 bytes, checksum: fe0f88eeb84f455dbeac9680e1d83210 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | Table of Contents
Acknowledgement I Abstract II Nomenclature IV Table of Content V List of Tables VIII List of Figures IX Chapter 1 Introduction 1 1.1 General Remarks 1 1.2 Motivation and Objectives 3 1.3 Literature Survey 6 1.3.1 Functional Probes for DNA Detection 6 1.3.2 Polymeric Materials for Biosensor 9 1.3.3 Multilayer RF Components for Biosensor 12 1.4 Contributions 14 1.5 Thesis Outline 14 Chapter 2 Design of the Polymeric RF biosensor 22 2.1 Selection of the Polymeric Materials for RF Application 22 2.2 Principles of the RF Biosensor 23 2.2.1 Passive Filters for Biosensor 23 2.2.2 Scattering Parameters 24 2.2.3 Definition of the center frequency 25 2.3 Design of the biosensor structures 26 2.4 Electromagnetic Simulation for the polymeric RF Biosensor 28 2.4.1 Simulation Tool 28 2.4.2 Simulation Procedures and Parameters for Biosensor 29 Chapter 3 Experimental Design and Fabrication Process 38 3.1 Micromachining Process Flow of the Polymer Biochip 39 3.1.1 CVD Process for Parylene Deposition 41 3.1.2 Isotropic Wet Etching for Metal Layer 42 3.2 Surface Treatment of the Biosensor 42 3.2.1 Surface Treatment with Silane A-174 43 3.2.2 Surface Treatment with O2 Plasma 43 3.2.3 Surface Modification with Thioglycolic Acid 44 Chapter 4 Experimental Apparatuses and Procedures 63 4.1 Experimental and measurement apparatuses 63 4.1.1 Experimental apparatuses 63 4.1.2 Measurement apparatuses 64 4.2 Experimental Materials 65 4.3 Experimental Procedures 66 4.3.1 DNA Hybridization with Self-assembly Multilayer AuNPs 66 4.3.2 DNA Hybridization with Self-assembly Multilayer MNPs 67 Chapter 5 Results and Discussion 81 5.1 Measurement Results of the Polymer Biosensor 81 5.2 Measurement results of the DNA detection 83 5.2.1 DNA Detection with AuNPs 83 5.2.2 DNA Detection with MNPs 85 Chapter 6 Conclusions and Future Prospects 105 References 107 | |
| dc.language.iso | en | |
| dc.title | 應用於DNA檢測之高分子射頻生醫感測器之開發 | zh_TW |
| dc.title | Development of a Polymeric Radio Frequency Biosensor for DNA Detection | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 戴慶良,楊啟榮,李達生,蔡作敏 | |
| dc.subject.keyword | 高分子感測器,射頻,DNA檢測,功能性奈米顆粒,生物感測器, | zh_TW |
| dc.subject.keyword | Polymeric sensor,Radio frequency,DNA detection,Function nanoparticles,Biosensor, | en |
| dc.relation.page | 114 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2011-07-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 機械工程學研究所 | zh_TW |
| 顯示於系所單位： | 機械工程學系 | |
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