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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳昭瑩(Chao-Ying Chen) | |
| dc.contributor.author | Yi-Ting Lien | en |
| dc.contributor.author | 連顗婷 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:09:30Z | - |
| dc.date.copyright | 2011-07-29 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-25 | |
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Pix Plus and mepiquat chloride effects on physiology, growth, and yield of field-grown cotton. J. Plant Growth Regul. 19: 415-422. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23734 | - |
| dc.description.abstract | 已知臘狀芽孢桿菌C1L菌株可以促進玉米植株生長，並有助於玉米抗南方葉枯病及臺灣百合、葵百合、阿卡波克百合抗灰黴病。為了發展可以穩定保存之製劑，本研究探討C1L內孢子的生產條件、製劑型式及應用功效。首先選擇C1L菌株產孢最適條件並擴大培養體積至3公升，以添加0.01%消泡劑之NB液態培養基進行發酵，培養2天的產孢率可達81%。將自發酵液所得的菌體冷凍乾燥並存放於室溫，計量之內孢子數由初始濃度1011 CFU/g至第十三個月仍維持在1010 CFU/g。由溫室試驗確認C1L內孢子促進玉米植株抗病之作用，以內孢子懸浮液處理1天後，對葉枯病即有明顯的防治效果，所誘導之抗病性可持續6天；並於菸草與阿拉伯芥上確認C1L內孢子與細菌懸浮液對植物之生長均有促進作用。進一步，選取多孔性載體試製C1L內孢子製劑，以吸附內孢子之真珠石有較佳的抑病作用；有機質肥料則可輔助吸附內孢子之真珠石的抑病作用。兩種測試之有機質肥料(I、II)無法增加植物抗病性，但可加強吸附內孢子之真珠石對植物抗病性的促進作用。玉米與葵百合之田間試驗結果指出，有機質肥料(I、II)可促進植株生長，但無法增加植株之抗病性；內孢子懸浮液不但能促進植株生長，並有很好的病害防治效果；當吸附內孢子之真珠石混拌有機質肥料I施用時，對植株生長、抗病的促進效果與澆灌內孢子懸浮液相當。本研究所建立之載體與輸送方式將可作為C1L菌株製劑化與應用之參考依據。 | zh_TW |
| dc.description.abstract | Bacillus cereus C1L could promote maize growth, induce disease resistance to southern corn leaf blight and Botrytis leaf blight in Lilium formosanum, Lilium ‘Star gazer’ and Lilium ‘Acapulco’. Previous studies demonstrated the great efficacy of strain C1L in promoting plant growth and inducing systemic resistance of maize against southern leaf blight in field conditions. This research aimed to produce endospores of high density, develop endospore-based bioagent and test on activities of plant growth promotion and induced systemic resistance. The endospores of strain C1L produced in 3-L culture with 0.01% anti-foaming agent reached about 81% sporulation rate after 2-day incubation. The freeze-dried powder of 1011 endospores per gram, maintained 1010 endospores per gram over 13-month storage period. In greenhouse assays, application of endospore suspensions could reduce disease severity of southern corn leaf blight when fungal pathogen was challenge-inoculated 1-6 days after bacterial treatment. Seed bacterization of tobacco and Arabidopsis with 1´108 CFU/ml of C1L endospores as well as vegetative cells significantly conducted better plant height, dry weight, and root length as compared to the untreated control. Porous media were tested as carriers of C1L endospores. Among them, perlite was best for delivery of C1L endospores as shown by the biocontrol assay in maize. Two kinds of organic fertilizers (I and II) were unable to enhance disease resistance of maize. However, combined application with organic fertilizer I could increase the effect of endospore-carried perlite on the induction of systemic resistance against southern corn leaf blight. The field assays of maize and Lilium ‘Star Gazer’ indicated that organic fertilizers could promote plant growth, but unable to reduce disease severity. However, application of endospore-carried perlite not only conferred better plant protection, but also promoted plant growth as applied with organic fertilizer I. The delivery system of C1L endospores developed in this study would become a valuable basis for the formulation and application of B. cereus C1L. | en |
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| dc.description.tableofcontents | 壹、中文摘要 1
貳、英文摘要 2 參、前言 4 肆、前人研究 6 一、植物促生根圈細菌 6 二、根圈細菌促進植物生長與生物防治之機制 6 三、芽孢桿菌屬之內孢子與其應用 8 四、細菌性農用生物製劑之載體研究 9 五、B. cereus農用生物製劑之應用發展 10 伍、材料與方法 11 一、供試菌株之培養與保存 11 1. 供試菌株之培養 11 2. 供試菌株之保存 11 二、C1L菌株最適產孢條件之探討 11 1. 不同培養基對於C1L菌株產孢之影響 11 2. 起始pH值對C1L菌株產孢之影響 12 3. 消泡劑對於C1L菌株產孢之影響 12 三、C1L菌株形成內孢子與內孢子特性探討 13 1. 內孢子形成之觀察 13 2. 臘狀芽孢桿菌C1L內孢子對80℃之耐受性測試 13 3. 比較C1L菌株與內孢子於玉米根內纏據之試驗 13 四、內孢子生長活性測試 14 五、C1L菌株促進植物生長試驗(模式植物) 14 1. 溫室試驗之植株栽培 14 2. C1L菌液與內孢子懸浮液對菸草生長之影響 15 3. C1L菌液與內孢子懸浮液對阿拉伯芥生長之影響 15 六、溫室病害防治試驗(模式植物) 15 1. 溫室試驗之植株栽培 15 2. 罹病嚴重度與統計方式 16 3. C1L內孢子對玉米之ISR作用 16 七、內孢子製劑對玉米之ISR作用 17 1. 不同載體製備內孢子製劑對玉米之ISR作用 17 2. 添加有機質肥料對內孢子製劑之作用 17 八、C1L內孢子製劑之田間玉米試驗 17 1. 促進植株生長試驗 17 2. 病害防治試驗 18 九、C1L內孢子製劑之田間葵百合試驗 18 1. 促進植株生長試驗 18 2. 病害防治試驗 18 陸、結果 20 一、臘狀芽孢桿菌C1L菌株最適產孢條件之探討 20 1. 不同液態培養基對C1L菌株產孢之影響 20 2. 不同起始pH值對C1L菌株產孢之影響 20 3. 不同濃度消泡劑對C1L菌株產孢之影響 20 二、C1L菌株內孢子特性測試 21 1. 生長曲線與產孢觀察 21 2. C1L內孢子對於高溫耐受性試驗 21 3. 施用C1L內孢子之玉米根內細菌數計量 22 三、內孢子生長活性測試 22 四、C1L菌株促進植物生長試驗(模式植物) 22 五、溫室防治試驗(模式植物) 23 1. 不同濃度之內孢子懸浮液對於玉米之ISR作用 23 2. 澆灌C1L內孢子懸浮液對於玉米之ISR持續性試驗 23 3. 不同載體製備內孢子製劑對ISR作用之影響 24 4. 有機質肥料對內孢子製劑ISR作用之影響 24 六、玉米田間試驗 24 1. C1L內孢子製劑對玉米生長之影響 25 2. 施用C1L內孢子製劑對南方玉米葉枯病之防治試驗 26 七、葵百合田間試驗 26 1. C1L內孢子製劑對葵百合生長之影響 26 2. 施用C1L內孢子製劑對百合灰黴病之防治試驗 27 柒、討論 28 捌、參考文獻 32 玖、圖表集 41 表一、臘狀芽孢桿菌C1L菌株於玉米根內纏據試驗 42 表二、臘狀芽孢桿菌C1L菌株處理圓葉菸草種子之促進生長效果 43 表三、臘狀芽孢桿菌C1L內孢子懸浮液處理阿拉伯芥種子之促進生長效果 44 表四、臘狀芽孢桿菌C1L內孢子懸浮液誘導玉米產生系統性抗病之持續性測試 45 表五、以多孔性載體吸附臘狀芽孢桿菌C1L內孢子對南方玉米葉枯病之防治效果 46 表六、施用臘狀芽孢桿菌內孢子製劑對玉米生長之影響(田間試驗) 47 圖一、不同液態培養基對臘狀芽孢桿菌C1L菌株產孢之影響 48 圖二、不同起始pH值對臘狀芽孢桿菌C1L菌株產孢之影響 49 圖三、不同濃度消泡劑對臘狀芽孢桿菌C1L菌株產孢之影響(50毫升培養基) 50 圖四、不同濃度消泡劑對臘狀芽孢桿菌C1L菌株產孢之影響(100毫升培養基) 51 圖五、不同濃度消泡劑對臘狀芽孢桿菌C1L菌株產孢之影響(3公升培養基) 52 圖六、臘狀芽孢桿菌C1L菌株之生長曲線與產孢數 53 圖七、臘狀芽孢桿菌C1L內孢子之孔雀綠染色圖 54 圖八、臘狀芽孢桿菌C1L內孢子於80℃下之存活率 55 圖九、室溫下儲存之臘狀芽孢桿菌內孢子生長活性測試 56 圖十、不同濃度內孢子懸浮液對玉米之ISR作用 57 圖十一、臘狀芽孢桿菌C1L內孢子懸浮液與菌液誘導玉米之系統性抗病效果比較 58 圖十二、臘狀芽孢桿菌C1L內孢子懸浮液誘導玉米系統性抗病之持續性測試 59 圖十三、於溫室測試臘狀芽孢桿菌C1L內孢子製劑對南方玉米葉枯病之防治效果(有機質肥料I) 60 圖十四、於溫室測試臘狀芽孢桿菌C1L內孢子製劑對南方玉米葉枯病之防治效果(有機質肥料II) 61 圖十五、於田間測試臘狀芽孢桿菌C1L內孢子製劑對玉米果實鮮重之影響 63 圖十六、施用臘狀芽孢桿菌C1L內孢子製劑之田間玉米葉片外觀 64 圖十七、於田間測試臘狀芽孢桿菌C1L內孢子製劑對南方玉米葉枯病之防治效果 66 圖十八、田間測試臘狀芽孢桿菌C1L內孢子製劑對葵百合生長之影響 67 圖十九、田間測試臘狀芽孢桿菌C1L內孢子製劑對葵百合花苞數之影響 68 圖二十、田間測試臘狀芽孢桿菌C1L內孢子製劑對百合灰黴病之防治效果 69 | |
| dc.language.iso | zh-TW | |
| dc.title | 臘狀芽孢桿菌C1L內孢子製劑之發展與應用 | zh_TW |
| dc.title | Development and application of Bacillus cereus C1L endospore-based bioagents | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳文希(Wen-Shi Wu),謝廷芳(Ting-Fang Hsieh),黃祥恩(Hsiang-En Huang) | |
| dc.subject.keyword | 植物促生根圈細菌,生物防治,內孢子製劑,載體,輸送系統, | zh_TW |
| dc.subject.keyword | Bacillus cereus,biocontrol,endospore formulation,carrier,delivery system, | en |
| dc.relation.page | 69 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2011-07-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| 顯示於系所單位： | 植物病理與微生物學系 | |
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