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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳信銘 | |
| dc.contributor.author | Yu-Ping Hsieh | en |
| dc.contributor.author | 謝郁平 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:59:29Z | - |
| dc.date.copyright | 2010-09-09 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-18 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23344 | - |
| dc.description.abstract | 口腔黏膜下纖維化症( Oral submucous fibrosis，OSF)是一種慢性發炎的口腔黏膜病變， 因嚼食檳榔而使口腔黏膜下的結締組織層產生過多的膠原蛋白，並過度堆積所造成的潛在性癌變障礙。 在許多研究中發現，在肺臟、肝臟、皮膚的纖維化症疾病中，其纖維母細胞都有過度的α-Smooth muscle actin (α-SMA)表現。因此，本研究的目的在探討 α-SMA與口腔黏膜下纖維化症致病機轉的關聯性。首先我們利用α-SMA抗體進行免疫組織化學染色法，偵測α-SMA在正常口腔頰黏膜組織(normal oral mucosa；NOM )與口腔黏膜下纖維化症組織(OSF)的表現情形。結果發現 NOM組織只在血管的平滑肌細胞( positive control )有表現α-SMA ，而OSF 組織其纖維母細胞卻有α-SMA 過度表現。其次，我們將NOM纖維母細胞以TGF-β或Arecoline處理之後， 萃取其蛋白質以西方墨點法分析之，發現這兩者都能誘導 α-SMA 的表現量增加， 並且呈現 dose-dependent 的情形。 最後， 我們利用 Curcumin和Epigallocatechin-3-gallate ( EGCG )， 以及 Histone deacetylase (HDAC) 的抑制劑 Trichostatin A (TSA) 這幾種藥物作用於NOM纖維母細胞， 發現它們都能抑制Arecoline或TGF-β誘導α-SMA的表現量增加。 在本研究中，我們證實了口腔黏膜下纖維化症的纖維母細胞會比正常口腔頰黏膜纖維母細胞表現較多的α-SMA。而Arecoline 和TGF-β 確實可以誘導口腔頰黏膜纖維母細胞表現較多的α-SMA蛋白質，最後我們發現EGCG、Curcumin 及TSA都可以有效的降低正常口腔頰黏膜纖維母細胞α-SMA蛋白質的表現量。
關鍵字：口腔黏膜下纖維化症、OSF、α-SMA | zh_TW |
| dc.description.abstract | Oral submucous fibrosis (OSF) is a chronic and inflammatory disease of oral mucous. Epidemiological evidences strongly indicate the association of chewing areca quid habit and OSF which is a kind of potentially malignant disorders. Many evidences have showed that α-smooth muscle actin (α-SMA) is overexpressed in some organs, such as lung, liver, and skin, but the α-SMA expression in OSF is not clear, therefore, in this study, we investigated the correlation of α-SMA expression and the pathogenesis in OSF by immunohistochemistry (IHC). The result shows that the expression of α-SMA in normal buccal mucosa only exists in the smooth muscle cells (positive control) of blood vessels, however, α-SMA is overexpressed in OSF. For detecting the effect of arecoline and TGF-β in OSF we treated normal oral mucous fibroblasts (NOM) with above components, and then analyzed the expression of α-SMA by Western Blot. The result showed that both arecoline and TGF-β were able to induce the expression of α-SMA in the dose-dependent manner. Finally, we treated NOM with Curcumin, Epigallocatechin-3-gallate (EGCG), or Trichostatin A (TSA), the result showed that all of them were able to reduce the expression of α-SMA induced by arecoline or TGF-β. In our study, we found that OSF expressed much more α-SMA than NOM. Besides, both arecoline and TGF-β can induce the expression of α-SMA. The induced expression of α-SMA by arecoline or TGF-β can be reduced by Curcumin, EGCG, or TSA.
Keywords: oral submucous fibrosis, OSF, α-SMA | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:59:29Z (GMT). No. of bitstreams: 1 ntu-99-R97450016-1.pdf: 1563980 bytes, checksum: 4c1b9868b7a3f63fd0c921a6d5066a5a (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 中文摘要...........................................................................................................................1
Abstract.............................................................................................................................2 第一章 導論.....................................................................................................................3 第一節 口腔黏膜下纖維化症.................................................................................3 1-1 口腔黏膜下纖維化症的簡介....................................................................3 1-2 口腔黏膜下纖維化症的組織學................................................................3 1-3 口腔黏膜下纖維化症的致病機轉............................................................3 1-4 口腔黏膜下纖維化症的文獻回顧............................................................4 第二節 α-SMA(α-smooth muscle actin)..................................................................7 2-1 α-SMA的簡介............................................................................................7 2-2 α-SMA的文獻回顧....................................................................................7 第三節 薑黃素 ( Curcumin ).................................................................................11 第四節 兒茶素EGCG............................................................................................12 第二章 實驗目的...........................................................................................................14 第三章 實驗材料與方法...............................................................................................15 第一節 實驗組與控制組檢體的收集...................................................................15 第二節 免疫組織化學染色法 ( Immunohistochemical stain ) ...........................15 第三節 人類頰黏膜纖維母細胞之初代培養.......................................................17 第四節 人類頰黏膜纖維母細胞之繼代培養.......................................................17 第五節 藥物的處理...............................................................................................18 第六節 細胞毒性試驗...........................................................................................18 第七節 西方墨點法...............................................................................................18 第八節 統計方法...................................................................................................20 第四章 結果...................................................................................................................21 OSF比NOM表現較多的α-SMA.........................................................................21 免疫組織化學染色之統計分析.............................................................................21 不同濃度的Arecoline影響NOM生長的情形.....................................................21 Arecoline會誘導α-SMA蛋白質的表現量增加.................................................. 22 Curcumin和EGCG會抑制Arecoline誘導α-SMA蛋白質的表現....................22 TGF-β會誘導α-SMA蛋白質的表現量增加.........................................................23 TSA會抑制TGF-β誘導α-SMA蛋白質的表現...................................................23 Curcumin會抑制TGF-β誘導α-SMA蛋白質的表現...........................................24 EGCG會抑制TGF-β誘導α-SMA蛋白質的表現.................................................24 不同濃度的TSA、Curcumin、EGCG影響NOM生長的情形...............................25 第五章 討論...................................................................................................................26 第六章 總結...................................................................................................................30 第七章 圖與表...............................................................................................................32 第八章 Reference...........................................................................................................46 | |
| dc.language.iso | zh-TW | |
| dc.title | 口腔黏膜下纖維化症中α-SMA表現之研究 | zh_TW |
| dc.title | The Research of α-SMA Expression in
Oral Submucous Fibrosis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 郭彥彬 | |
| dc.contributor.oralexamcommittee | 江俊斌 | |
| dc.subject.keyword | 口腔黏膜下纖維化症,OSF,α-SMA, | zh_TW |
| dc.subject.keyword | oral submucous fibrosis,OSF,α-SMA, | en |
| dc.relation.page | 54 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2010-08-18 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 目前未授權公開取用 | 1.53 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。