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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 廖秀娟 | |
| dc.contributor.author | Wen-Hsuan Li | en |
| dc.contributor.author | 李汶軒 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:48:49Z | - |
| dc.date.copyright | 2009-07-31 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-28 | |
| dc.identifier.citation | Allan CB, Lacourciere GM, Stadtman TC (1999) Responsiveness of selenoproteins to dietary selenium. Annu Rev Nutr 19: 1-16
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23232 | - |
| dc.description.abstract | 硒對於哺乳類動物是一個已知的微量必需元素，並且對於細胞的反應相當重要。作為selenoprotein的成分之一，硒有其結構上和酵素作用的角色，具有抗氧化和癌症的化學保護能力。本研究利用秀麗隱桿線蟲作為模式生物用以了解硒是如何影響線蟲。首先透過建立dose-response去了解硒的毒性，發現硒對秀麗隱桿線蟲的致死濃度為2.785 mM。在0.01和0.05 μM低劑量硒的暴露下，硒具有加速秀麗隱桿線蟲發育和增強抵抗可抑制神經傳遞訊息化學物質所造成的癱瘓的能力。然而，在20 μM的較高劑量暴露下，硒反而會對於發育產生明顯延遲的效應，並對生育力、體長和壽命造成顯著的減少。此外本研究也發現，20 μM的硒會對於由aldicarb和levamisole此兩種藥物所產生的癱瘓有更為敏感的影響，但0.01 μM 的硒提供線蟲抵抗、保護此癱瘓的能力。本研究說明硒對於發育、生育力、壽命和神經系統具有有利(或保護)和不利的特質。 | zh_TW |
| dc.description.abstract | Selenium (Se) is an universal essential trace element for mammals which is important for many cell processes. As a constituent of selenoproteins, Se has structural and enzymic roles and it has been recognized as an antioxidant and chemopreventive agent in cancer. In this study, we use a model organism Caenorhabditis elegans to understand how Se affects the nematodes. To understand the effects of Se(IV) on C. elegans, we established the dose response relationship and found that the LC50 of Se was 2.785mM. Low level of Se (0.01 and 0.05 μM) accelerated the development rate and enhanced the resistances to the paralysis caused by the chemicals related to cholinergic signaling. However, at the addition of 20 μM, Se caused significant delays of development and serious decreases in fertility, body sizes and life span. It caused the worms more hypersensitive to the paralysis caused by aldicarb and levamisole. But at the supplementation of 0.01 μM, Se could provide resistance to the paralysis. Our results showed that Se has both beneficial (or protective) and adverse properties on development, fertility, growth, lifespan, and nervous system. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:48:49Z (GMT). No. of bitstreams: 1 ntu-98-R96622045-1.pdf: 929215 bytes, checksum: 56264f428a30520c01d026ba62f23489 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | TABLE OF CONTENTS
致謝 I 中文摘要 III ABSTRACT IV TABLE OF CONTENTS V LIST OF TABLES VIII LIST OF FIGURES IX ABBREVIATION XI CHAPTER 1 INTRODUCTION 1 1.1The importance of essential trace elements 1 1.2Selenium 2 1.3Protective and essential roles for Se 4 1.4The toxicity of Se 11 1.5Caenorhabditis elegans as model organism 14 1.6Purpose of study 18 CHAPTER 2 MATERIALS AND METHODS 20 2.1Chemicals, strains, and culture condition 20 2.2Development synchronization 20 2.3Toxicity test 20 2.4Locomotion behavior assay 21 2.5Brood size 22 2.6Lifespan assay 23 2.7Morphology change 23 2.8Measurement of internal Se levels using ICP 23 2.9Monitoring development of the worms in the presence of exogenous Se(IV) 24 2.10Paralysis assay 25 CHAPTER 3 RESULTS 26 3.1Toxicity tests of Se(IV) and establishment of LC50 value 26 3.2The influence of Se(IV) on the locomotion of C. elegans 26 3.3The effects on the growth in the presence of exogenous Se(IV) 30 3.4Exogenous Se(IV) at certain concentrations decreases hermaphrodite self-fertility of wild type 33 3.5The changes of body size under Se exposure at certain concentrations 35 3.6Lifespan of worms is decreased by Se(IV) treatment 38 3.7Se accumulation in wild type worms as determined by ICP 41 3.8Different fates of locomotion of worms exposed to aldicarb or levamisole under Se(IV) pretreatment 43 CHAPTER 4 DISCUSSION 50 CHAPTER 5 CONCLUSION & FUTURE DIRECTION 58 REFERENCES 59 LIST OF TABLES Table 1 Mean life span of wild type worms with Se(IV) supplement 40 LIST OF FIGURES Fig. 1.1 Schematic representation of the Se metabolic pathway 5 Fig. 1.2 Dependence of biological functions on tissue concentration on intake of a nutrient 12 Fig. 1.3 Diagram of C. elegans 15 Fig. 1.4 Life cycle of C. elegans 17 Fig. 3.1 C. elegans Se(IV) toxicity assay 27 Fig. 3.2 The influence of Se toxicity on the locomotion behavior of C. elegans at 72-hour exposure 29 Fig. 3.3 Effect of Se(IV) supplementation on the development of wild type worms from egg to adulthood at 22℃ 32 Fig. 3.4 Effect of Se(IV) on the fertility of wild type worms at 22℃ 34 Fig. 3.5 (A) Influence of Se(IV) on growth of wild type animals by measuring the body size at 22℃ 36 Fig. 3.5 (B) Morphological change associated with C. elegans exposed to various concentrations of Se(IV) 37 Fig. 3.6 Effects of Se(IV) supplementation on the adult life span of wild type C. elegans hermaphrodites at 22℃ 39 Fig. 3.7 Internal Se concentration determination using ICP 42 Fig. 3.8 Locomotion behavior changes of worms exposured to 1mM aldicarb under 24 hr Se(IV) pretreatment at 22℃ 46 Fig. 3.9 Locomotion behavior changes of worms exposured to 300 μM levamisole under 24 hr Se(IV) pretreatment at 22℃ 48 | |
| dc.language.iso | en | |
| dc.subject | 有利 | zh_TW |
| dc.subject | 硒 | zh_TW |
| dc.subject | 不利 | zh_TW |
| dc.subject | 秀麗隱桿線蟲 | zh_TW |
| dc.subject | Se | en |
| dc.subject | adverse | en |
| dc.subject | beneficial | en |
| dc.subject | Caenorhabditis elegans | en |
| dc.title | 微量元素無機四價硒對秀麗隱桿線蟲
之有益及毒性效應探討 | zh_TW |
| dc.title | The Ameliorative and Toxic Responses of Selenite in the Nematode Caenorhabditis elegans | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 羅時成,陳昌熙,陳佩貞 | |
| dc.subject.keyword | 硒,秀麗隱桿線蟲,有利,不利, | zh_TW |
| dc.subject.keyword | Se,Caenorhabditis elegans,beneficial,adverse, | en |
| dc.relation.page | 69 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2009-07-29 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物環境系統工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物環境系統工程學系 | |
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