番茄中的S-adenosylmethionine decarboxylase (SAMDC) 和Wun1基因對於雄蕊發育和花粉萌發的影響

Ming-Yuan Cheng; 鄭名淵

請用此 Handle URI 來引用此文件： http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23095

完整後設資料紀錄

DC 欄位	值	語言
dc.contributor.advisor	鄭石通
dc.contributor.author	Ming-Yuan Cheng	en
dc.contributor.author	鄭名淵	zh_TW
dc.date.accessioned	2021-06-08T04:41:40Z	-
dc.date.copyright	2009-08-14
dc.date.issued	2009
dc.date.submitted	2009-08-11
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dc.identifier.uri	http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23095	-
dc.description.abstract	番茄雄蕊在高溫底下發育會受損，花粉萌發率及著果率也會跟著下降，實驗室之前利用Suppression Subtractive Hybridization (SSH)所建立之cDNA library中，已找到會被熱誘導之基因S-adenosylmethionine synthetase (SAMS) 和 Wun1基因。S-adenosylmethionine decarboxylase (SAMDC)基因是SAMS的下游基因，被認為是製造多元胺之關鍵酵素，而多元胺含量則對於花朵發育有很重要的影響，但多元胺對於雄蕊之發育影響之研究卻極為有限。目前研究指出，多元胺參與對抗多種非生物性逆境，包含鹽類、低溫、酸性逆境等，而SAMDC的大量表現，卻可以抵抗這些生物逆境，關於多元胺及溫度對於花發育的影響目前的了解都極有限。為探討SAMDC基因的功能，本研究以轉殖番茄的方式大量表現SAMDC，以研究此基因的表現對於雄蕊和花粉發育的影響。但有研究指出若僅表現SAMDC基因主要編碼區而沒有表現其上游編碼區(uORF)，則可能會造成毒性讓植物死亡，因此進而製作了包含及不包含uORF的兩個SMADC基因構築。同時，文獻指出以35S啟動子過量表現SAMDC，植物的生長情況會比野生型快速，為了避免營養器官生長造成之影響，選用雄蕊專一表現啟動子將之表現在不耐熱番茄4783中，結果發現，不含上游閱讀框架的SAMDC轉殖番茄花朵無法正常發育；包含uORF的SAMDC轉殖番茄則會產生較多的花粉、較好之花粉萌發率且高溫下萌發花粉管生長情形良好，而花粉產量提升可能因為SAMDC表現量增加進而造成多元胺增加，加速細胞週期的進行，導致花粉數目增加。本研究之另一個對象Wun1是經傷害後會大量表現的蛋白，推測其功能為可保護植物細胞壁，其表現也會受到高溫的誘導，但是在4783以轉殖番茄的方式大量表現Wun1在植物中，卻發現轉殖植物的花粉萌發率降低，且花粉的型態發生改變，可能是因為callose生合成受影響所造成。	zh_TW
dc.description.provenance	Made available in DSpace on 2021-06-08T04:41:40Z (GMT). No. of bitstreams: 1 ntu-98-R96b42013-1.pdf: 1466113 bytes, checksum: 329e13248a4cea8ee7d7881c2747ca3d (MD5) Previous issue date: 2009	en
dc.description.tableofcontents	目錄中文摘要.............................................................................................................I 英文摘要.............................................................................................................II 縮寫與中英對照表…………………………………………………………...III 第一章前言.....................................................................................................1 一、番茄與熱境.........................................................................................1 二、高溫生長下雄蕊差異表現基因之分離………….............................1 三、在番茄中具有耐熱潛力之基因.........................................................2 四、多元胺（polyamine）與SAMDC (S-adenosyl-methionine decarboxylase) …………………………………………………………………………….2 五、TomA 108啟動子…………………………………………………………7 六、Wun1………………………………………………………………………7 七、研究目的與方向………………………………………………….…7 第二章材料與方法.........................................................................................9 一、材料.....................................................................................................9 二、番茄雄蕊RNA的萃取......................................................................9 三、聚合酵素連鎖反應(The polymerase chain reaction, PCR)及real-time PCR……………………..…..............................................................10 四、多元胺之萃取與測量……………………………………..…….....12 五、質體構築與挑選………………………………..……………….....13 六、轉殖番茄所用之質體構築………………………………….…......15 七、農桿菌之轉形……………………………....………………….......16 八、轉殖番茄………………………………………..……..….…..........17 九、植物基因組DNA抽取…………………………...…….…………19 十、番茄花粉外表型的觀察………………………………...……........20 第三章結果............................................................................................................22 I SAMDC之功能研究…………………………………………………...22 一、不同溫度下生長之番茄雄蕊，在未開花期的SAMDC基因表現量及多元含量.……......….……………………………………….....22 二、確認溫度對TomA 108啟動子的影響………………………….…23 三、番茄轉殖用載體的構築………….……………….……….…….....23 四、目標基因於轉殖番茄的篩選與表現……………….……….….…24 五、雄蕊內多元胺與乙烯生合成路徑各基因表現分析………….......27 II Wun1之功能研究…….………..………………………….……….….27 一、番茄轉殖用載體的構築………….………….…….……………......27 二、目標基因於轉殖番茄的篩選與表現……………….………...........28 第四章討論……………………………………………………….………...31 一、高溫對於番茄雄蕊的影響……..……..……..……………….…….31 SAMDC之功能探討..……………………………….………………..…31 二、溫度對於番茄雄蕊SAMDC表現及多元胺多寡之影響………….…....31 三、SAMDC對於雄蕊及花粉發育之探討………………………………....33 四、SAMDC對於耐熱性質之探討………………………………………..…..34 五、番茄雄蕊內多元胺與乙烯生合成路徑之基因交互關係…………..……35 Wun1之功能探討 .………….…………………….……………………36 六、Wun1對於耐熱性質之探討…………………………………………..36 七、結論………………………………………………………………...37 第五章參考文獻………………………………………….………….....….39 圖表…………………………………………….……………………………..46
dc.language.iso	zh-TW
dc.title	番茄中的S-adenosylmethionine decarboxylase (SAMDC) 和Wun1基因對於雄蕊發育和花粉萌發的影響	zh_TW
dc.title	Effect of S-adenosylmethionine decarboxylase (SAMDC) and Wun1 genes on stamen development and pollen germination in tomato	en
dc.type	Thesis
dc.date.schoolyear	97-2
dc.description.degree	碩士
dc.contributor.oralexamcommittee	林讚標,鄭秋萍,張孟基,洪傳揚
dc.subject.keyword	番茄,熱,多元胺,雄蕊,花粉,	zh_TW
dc.subject.keyword	tomato,heat,polyamine,stamen,pollen,	en
dc.relation.page	66
dc.rights.note	未授權
dc.date.accepted	2009-08-11
dc.contributor.author-college	生命科學院	zh_TW
dc.contributor.author-dept	植物科學研究所	zh_TW
顯示於系所單位：	植物科學研究所

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