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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭石通 | |
| dc.contributor.author | Fang-Yi Chiang | en |
| dc.contributor.author | 江芳怡 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:39:11Z | - |
| dc.date.copyright | 2009-08-18 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-14 | |
| dc.identifier.citation | 王怡潔 (2006). 耐熱番茄與不耐熱番茄中SAM3與SUS3基因之分析及比較。國立台灣大學植物科學研究所碩士論文。
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G., Hoffmann, G.F., Braugitan, C., Wevers, R. A., and Gennip, A. H. V. (2001). Dectection of β-Ureidopropionase deficiency with HPLC-electrospray tandem mass spetectrometry and cocfirmation of the defect at the enzyme level. J. Inherit. Metab. Dis. 24: 725-732. Mayer, R. R., Cherry, J. H., and Rhodes, D. (1990). Effects of heat shock on amino acid metabolism of cowpea cells. Plant Physiol. 94:796-810. Moore, E.L., and Thomas, W.O. (1952). Some effects of shading and para-chlorophenoxy acetic acid on fruitfulness of tomatoes. Proceedings of the Amer. Soc. for Hor. Sci. 60: 289-294. Ohse, M., Matsuo, M., Ishida, A., and Kuhara, T. (2002). Screening and diagnosis of β-ureidopropionase deficiency by gas chromatographic/mass spectrometric analysis of urine. J. Mass Spectrom. 37: 954-962. Polowick, P. L., Boloria, R., and Sawhney, V. K. (1990). Stamen ontogeny in the temperaturesensitive 'stamenless-2' mutant of tomato (Lycopersicon esculentum L.). New Phytol. 115: 625-631. Peet, M. M., Sato, S., and Gardner, R.G. (1998). Comparing heat stress effects on male-fertile and male-sterile tomatoes. Plant Cell Environ. 21: 225-231. Rizhsky, L, Liang, H, Shuman, J, Shulaev, V, Davletova, S, and Mittler, R. (2004). When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiol. 134:1683-1696. Rychlik, M. (2000). Quantification of free and bound pantothenic acid in foods and blood plasma by a stable isotope dilution assay. J. Agric. Food Chem. 48: 1175-1181. Santos, H., and da Costa, M.S. (2002). Compatible solutes of organisms that live in hot saline environments. Environ. Microbiol. 4: 501-509. Sato, S., Kamiyama, M., Iwata, T., Makita, N., Furukawa, H., and Ikeda, H. (2006). Moderate increase of mean daily temperature adversely affects fruit set of Lycopersicon esculentum by disrupting specific physiological processes in male reproductive development. Ann. Bot. 97: 731-738. Schwacke, R., Grallath, S., Breitkreuz, K.E., Stransky, E., Stransky, H., Frommer, W.B., and Rentsch, D. (1999). LeProT1, a transporter for proline, glycine betaine, and gamma-amino butyric acid in tomato pollen. Plant Cell 11: 377-391. Song, J., Nada, K., and Tachibana, S. (2001). The early increase of S-adenosylmethionine decarboxylase activity is essential for the normal germination and tube growth in tomato (Lycopersicon esculentum Mill.) pollen. Plant Sci. 161: 507-515. Spry, C., Kirk, K., and Saliba, K. J. (2008). CoenzymeA biosynthesis: an antimicrobial drug target. FEMS Microbiol. Rev. 32: 56-106. Walsh, T. A., Green, S. B., Larrinua, I. M., and Schmitzer, P.R. (2001). Characterization of plant β-ureidopropionase and fumctional overexpression in Escherichia coli. Plant Physiol. 125: 1001-1011. Willits, D.H., and Peet, M.M. (1998). The effect of night temperature on greenhouse grown tomato yields in warm climates. Agri. Forest Meteorol. 92: 191-202. Zrenner, R., Riegler, H., Marquard, C. R., Lange, P. R., Geserick, C., C. Bartosz, E., Chen, C. T., and Slocum, R. D. (2009). A functional analysis of the pyrimidine catabolic pathway in Arabidopsis. New Phytol. 183: 117-132. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23044 | - |
| dc.description.abstract | 番茄在熱帶及亞熱帶區域夏季的生長情形和著果率，明顯較溫帶區域或冬季差，前人研究指出，高溫會影響花粉和雄蕊的發育，導致番茄產量下降。為了分析影響花粉活性的熱干擾基因為何，本實驗室先前已藉由抑制性扣減雜交法建立 cDNA 基因庫，從耐熱品系 5915 番茄中分離出數個 cDNA 片段，並且利用 real-time PCR 和北方墨點法分析溫度對這些基因的影響。本研究挑選在日溫 35℃/ 夜溫 30℃ 下的熱誘導基因 Clone 7 做進一步的研究。經由 NCBI 蛋白質保守序列資料庫比對，得知 Clone 7 可能是阿拉伯芥的 β-ureidopropionase, PYD3 同源基因，它含有保守性的 C-N hydrolase 區域，功能為產生 β-alanine。藉由基因轉殖實驗，在不耐熱番茄品系 4783 及耐熱番茄品系 5915 中大量表現 Clone 7 基因，同時在耐熱番茄品系 5915 中抑制 Clone 7 基因的表現，植物種植在適溫及高溫逆境下進行分析比較。利用掃描式電子顯微鏡觀察番茄雄蕊筒內部構造，將轉殖株及野生型做比較，可觀察到 Clone 7基因的表現量與花粉粒外觀、雄蕊筒內側的絨毛數量及成熟花粉釋放處開裂的情形成正相關，推測花粉釋放處之開裂程度可能會影響花粉釋放進而影響到番茄的授粉率。而比較轉殖株和野生型雄蕊筒之鮮重與長度及檢測花粉萌發率得知，Clone 7基因的表現量和雄蕊筒鮮重與長度及花粉萌發率皆成正相關，因此熱誘導基因 Clone 7 可影響雄蕊筒的發育和花粉的萌發率。另外添加 β-alanine 至花粉萌發培養基中，發現 β-alanine 會增加 5915 野生型花粉在 35℃ 時的萌發率，本研究為第一個探討 β-alanine 與番茄生長發育之間的關係。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:39:11Z (GMT). No. of bitstreams: 1 ntu-98-R96b42007-1.pdf: 7239369 bytes, checksum: a22313cf1ca443929a07f8ccceab1913 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 目錄
中文摘要……………………………………………………………….………..…..I 英文摘要……………………………………………………………….………..….II 縮寫與中英對照表…………………………………………………………….…..III 第一章 前言 一、番茄與熱逆境…………………………………………….…………..1二、高溫生長下雄蕊筒差異表現基因之分離……..…………..….……2 三、在番茄中具有耐熱潛力之基因 Clone 7 ……………….………..….2 四、研究目的與方向……………………………………………….……..5 第二章 材料與方法 一、材料…………………………………………………..……........6 二、番茄轉殖用載體與目標基因於轉殖番茄的確認…………......6 三、轉殖番茄…………………………………………..…..….........9 四、抽取植物基因組（genomic）DNA 並檢測轉殖株…...........11 五、番茄雄蕊筒 RNA 的萃取……………………...…..………......12 六、RT（reverse transcriptase）PCR…………………………........13 七、比較番茄野生型與轉殖株之外表型………………..…..… ...13 第三章 結果 一、轉殖番茄………………………………..…….……………...16 二、轉殖株之耐熱性分析……….………………….……………...16 2.1 野生型與轉殖株生長狀況之比較….……….……………...16 2.2 野生型與轉殖株雄蕊筒內部發育之比較……...…………...16 2.3 花粉萌發與花粉管生長分析………………….…………....20 2.4 添加 β-alanine 可促進 5915 野生型高溫時之萌發率....22 2.5 測量野生型與轉殖株雄蕊筒重量及長度之比較……….….....22 第四章 討論 一、高溫對於番茄生長發育的影響………..……..………………….25 二、Clone 7 對於耐熱的功能探討………………………..….….26 三、Clone 7大量表現及抑制表現之轉殖株分析……….….……28 四、結論………………..………………………………..…..…….29 第五章 參考文獻………………………………………………….….………..…30 表一 Clone 7進行 PCR 所使用的引子序列……….………………..….....33 表二 轉殖株名稱、特性及其建構與分析者….………………………..…...34 圖一至圖十四..….………………………..…………………………...35-48 附圖一至附圖六..….………………………..…………………………...49-54 | |
| dc.language.iso | zh-TW | |
| dc.title | 番茄中熱干擾的 Clone 7 基因對於雄蕊發育和花粉萌發的影響 | zh_TW |
| dc.title | Effect of heat-interference Clone 7 gene on stamen development and pollen germination in tomato | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林讚標,鄭秋萍,張孟基,洪傳揚 | |
| dc.subject.keyword | 番茄,花粉,雄蕊筒,熱,β-丙氨酸, | zh_TW |
| dc.subject.keyword | tomato,pollen,stamen,heat,β-alanine, | en |
| dc.relation.page | 54 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2009-08-14 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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