請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23022
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳弘成(Hung-Cheng Chen) | |
dc.contributor.author | Jui-Kuo Kao | en |
dc.contributor.author | 高瑞國 | zh_TW |
dc.date.accessioned | 2021-06-08T04:38:10Z | - |
dc.date.copyright | 2009-08-19 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-15 | |
dc.identifier.citation | 冉繁華編,2007。石斑,台灣漁業經濟發展協會,行政農委員會。
胡興華,2000。話漁台灣,行政院農業委員會漁業署,P140-151。 吳清熊,1982。養魚和飼料脂質。水本水產學會編著。國立編譯館。 陳紫媖與蘇惠美,2005。水產種苗的生產。科學發展,385, 32-41。 漁業署,2006。漁業署年報。行政院農業委員會漁業署。 蘇惠美,1999。餌料生物之培養與利用。台灣省水產試驗所東港分所。 蘇惠美,蘇茂森,廖一久,1994。極小型輪蟲之篩選及培養條件。水產研究,2:19-29。 Bell, M.V., Batty, R.S., Dick, J.R., Fretwell, K., Navarro, J.C., Sargent, J.R., 1995. Dietary deficiency of docosahexaenoic acid impairs vision at low light intensities in juvenile herring (Clupea harengus L.). Lipids 30, 373–476. Carić, M., Sanko-Njire, J., Skaramuca, B., 1993. Dietary effects of different feeds on the biochemical composition of the rotifer (Brachionus plicatilis Müller). Aquaculture 112, 195-206 Darias, M.J., Zambonino-Infante, J.L., Hugot, K., Cahu, C.L., Mazurais, D., 2008. Gene Expression Patterns During the Larval Development of European Sea Bass (Dicentrarchus Labrax) by Microarray Analysis. Marine Biotechnology 10, 416–428 Fernández-Reiriz,, M.J., Labarta, U., Ferreiro, M.J., 1993. Effects of commercial enrichment diets on the nutritional value of the rotifer (Brachionus plicatilis). Aquaculture 112, 195-206 Fielder, D.S., Purser, G.J., Battaglene, S.C., 2000. Effect of rapid changes in temperature and salinity on availability of the rotifers Brachionus rotundiformis and Brachionus plicatilis. Aquaculture 189, 85-99 Hirayama, K., Watanabe, K., 1973. Fundamental studies on the physiology of rotifers in mass culture-IV. Nutritional effect of yeast on population growth of rotifer. Bulletin of the Japanese society of scientific fisheries. 39, 1129-1133. Koven, W.M., Tandler, A., Kissil, G., Sklan, D., Friezlander, O., Harel, M., 1990. The effect of dietary_n-3. polyunsaturated fatty acids on growth, survival and swim bladder development in Sparus aurata larvae. Aquaculture 91, 131–141. Leger, P.H., Bengtson, D.A., Simpson, K.L., Sorgeloos, P., 1986. The use and nutritional value of Artemia as a food source. Oceanography and Marine Biology, An Annual Review 24, 521-623. Mourente, G., Tocher, D., Sargent, J., 1991. Specific accumulation of docosahenaenoic acid (22:6(n-3)) in brain lipids during development of juvenile turbot Scophthalmus maximus L. Lipids 26, 871–877. Naz, M., 2008. The changes in the biochemical compositions and enzymatic activities of rotifer (Brachionus plicatilis, Muller) and Artemia during the enrichment and starvation periods. Fish physiology and biochemistry 34,391-404 Reitan, K.I., Rainuzzo, J.R., Oie, G., 1993. Nutritional effects of algal addition in first feeding of turbot (Scopthalnus maximus L.) larvae. Aquaculture 118,257–275 Reitan, K.I., Rainuzzo, J.R., Olsen, Y., 1994. Influence of lipid composition of live feed on growth, survival and pigmentation of turbot larvae. Aquaculture international : journal of the European Aquaculture Society 2, 33–48 Shields, R.J., Bell, J.G., Luizi, F.S., Gara, B., Bromage, N.R., Sargent, J.R., 1999. Natural Copepods Are Superior to Enriched Artemia Nauplii as Feed for Halibut Larvae (Hippoglossus hippoglossus) in Terms of Survival, Pigmentation and Retinal Morphology: Relation to Dietary Essential Fatty Acid. The Journal of Nutrition 129, 1186–1194. Su, H.-M., Su, M.-S., Liao, I.-C., 1994. Fatty acid composition of the rotifer Brachionus plicatilis fed selected microalgae or yeast alone or in combination with various oils. In L. M. Chou et al. (eds), The Third Asian Fisheries Forum. Asian Fisheries Society, Manila, pp.207-210. Toledo, J.D., Golez, M.S.N., Doi, M., Ohno, A., 1999. Use of copepod naupli in during the early feeding stage of grouper, Epinephelus coioides. Fisheries Science 65, 390-397. Watanabe, T., Oowa, F., Kitajima, C., Fujita, S., 1980. Relationship between dietary value of brine shrimp, Artemia salina, and their content of ω3 highly unsaturated fatty acids. Bulletin of the Japanese Society of Scientific Fisheries 46, 35-41. Watanabe, T., 1982. Lipid nutrition in fish. Comparative biochemistry and physiology B 73, 3-15. Watanabe, T., 1993. Importance of docosahexaenoic acid in marine larval fish. J World Aquacult Soc 24:152–161 Wu, F.-C., Ting, Y.-Y., Chen, H.-Y., 2002. Docosahexaenoic Acid Is Superior to Eicosapentaenoic Acid as the Essential Fatty Acid for Growth of Grouper, Epinephelus malabaricus. The Journal of nutrition 132, 72-79 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23022 | - |
dc.description.abstract | 石斑魚是台灣重要的高經濟養殖魚種,其魚苗培育中期的餌料生物多以豐年蝦與橈足類為主,為了解不同餌料生物對魚苗發育之影響,我們首先以離水測試餵食不同餌料生物之石斑魚苗其抗緊迫之存活率,結果得豐年蝦餵食組8.13%、超級熊克滋養豐年蝦餵食組72.28%、橈足類餵食組98.21%。進一步探討這些餵料生物與所餵食石斑魚魚的脂肪酸組成。在餌料生物方面,豐年蝦含,DHA 0.22%,EPA 1.80%,ARA 0.48%,超級熊克滋養豐年蝦含DHA 4.53%,EPA 5.12%,ARA 0.66%,橈足類含DHA 6.30%,EPA 8.48%,ARA 1.67%;在魚苗方面,餵食豐年蝦組DHA 1.66%,EPA5.24%,ARA 3.28%,餵食超級熊克滋養豐年蝦組DHA 7.14%,EPA 6.44%,ARA 3.81%,餵食橈足類組DHA 15.47%,EPA 7.13%,ARA 1.57%。其中只有餵食橈足類組在14天餵食期結束後完成魚苗變態。此結果顯示,食物鏈中的高度不飽和脂肪酸含量對於石斑魚苗的發育、變態、抗緊迫存活率有很高的影響。以微陣列分析餵食橈足類與豐年蝦之基因表現差異,發現2.5倍以上促進表現的基因有253 個,抑制表現的基因有123 個,這些差異性表現基因包括醣解作用相關的基因如:muscle-beta-nolase 3、pyruvate kinase、muscle-phosphoglycerate mutase 2-1、glyceraldehyde-3-phosphate dehydrogenase及Fructose-bisphosphate aldolase A等;與核酸合成,細胞調控相關的基因如:Nucleoside diphosphate kinase B、 nucleoside diphosphate kinase-Z1;與結構相關的基因如:lily-type 、lectinmuscle-specific beta 1 integrin binding protein 2及similar to Histone H1.5。 | zh_TW |
dc.description.abstract | Grouper is important culture fish with high economic value in Taiwan, the major living feed during the middle stage of grouper fry culture are artemia and Copepods. To understand the effect of different living feed on fish fry development, we first test the anti-stress survival rate that remove from water of grouper fry fed with different living feed, the survival rate of the group fed with artemia was 8.13%, the group fed with artemia enriched with super SELCO was 72.28%, and the group fed with Copepods was 98.21%. Then we further investigated the fatty acid composition of living feed and grouper. In the living feed, artemia contains DHA 0.22%, EPA 1.80%, ARA 0.48%; artemia enriched with super SELCO contains DHA 4.53%, EPA 5.12%, ARA 0.66%; Copepods contain DHA 6.30%, EPA 8.48%, ARA 1.67%. And in fish fry, the group fed with artemia contains DHA 1.66%, EPA 5.24%, ARA 3.28%, fed with artemia enriched with super SELCO contains DHA 7.14%, EPA 6.44%, ARA 3.81%, and the group fed with Copepods contains DHA 15.47%, EPA 7.13%, ARA 1.57%. In which, only the group fed with Copepods completes fry metamorphosis after 14 days of feeding period. The results showed that the content of highly unsaturated fatty acid in food chain has significant influence on the development, metamorphosis and anti-stress survival rate of grouper fry. We also used microarray to analyze the differential gene expression of fry fed with artemia and copepods, and found that there were 253 genes with up-regulated and 123 genes with down-regulated. These differentially expressed genes include glycosylation related genes: muscle-beta-nolase 3, pyruvate kinase, muscle-phosphoglycerate mutase 2-1, glyceraldehyde-3-phosphate dehydrogenase and fructose-bisphosphate aldolase A; nucleic acid synthesis and cellular regulatory related genes: nucleoside diphosphate kinase B and nucleoside diphosphate kinase-Z1; structural gene:lily-type, lectinmuscle-specific beta 1 integrin binding protein 2 and similar to Histone H1.5. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:38:10Z (GMT). No. of bitstreams: 1 ntu-98-R96b45024-1.pdf: 1487524 bytes, checksum: 51347fae6e420ea2265b228e351a871a (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 摘要 I
Abstract II 目錄 IV 表目次 VI 圖目次 VII 壹、 前言 1 1.1 臺灣養殖現況與石斑魚苗簡介 1 1.2 石斑魚苗孵育之餌料生物 2 1.2.1 輪蟲 3 1.2.2 豐年蝦 3 1.2.3 橈足類 4 1.3 餌料生物的高度不飽和脂肪酸組成 4 1.4 不飽和脂肪酸對魚苗發育的重要性 4 1.5 研究目的 5 貳、 材料與方法 7 2.1 生物性材料 7 2.2 不同食物條件餵食點帶石斑魚苗 7 2.3 石斑魚苗λ噬菌體cDNA基因庫之構築 8 2.3.1 石斑魚苗mRNA之萃取及RNA電泳 10 2.3.2 cDNA之合成與修飾 11 2.3.3 分離不同大小之cDNA片段 13 2.3.4 cDNA嵌入片段與載體之接合反應 13 2.3.5 λ噬菌體之包裝 13 2.3.6 宿主細胞之製備 14 2.3.7 石斑魚苗組織cDNA基因庫力價之測定 14 2.3.8 轉換噬菌體載體為質體載體 14 2.3.9 候選基因藍白篩選和質體純化 14 2.4 不同的餵食條件對魚苗的影響 15 2.5 不同食物與石斑魚苗的脂肪酸分析 15 2.5.1 粗脂肪萃取 16 2.5.2 皂化 16 2.5.3 甲基酯化 17 2.5.4 氣相層析 (Gas Chromatograph,GC) 17 2.6 cDNA微陣列之雜合反應 17 2.6.1 Total RNA之萃取、純化與RNA電泳 19 2.6.2 基因微陣列晶片之前準備 20 2.6.3 cDNA合成與標定 20 2.6.4 cDNA微陣列之雜合反應 21 2.6.5 候選基因定序及序列比對分析 21 參、 結果 23 3.1 石斑魚苗組織cDNA基因庫建立及力價之測定 23 3.2 不同的餵食條件對魚苗成長及抗緊迫之影響 23 3.3 餵食不同食物對石斑魚苗脂肪酸組成之影響 23 3.3.1 餵食不同食物對石斑魚苗粗脂肪比例之影響 23 3.3.2 餵食不同食物對石斑魚苗脂肪酸組成之影響 24 3.4 差異表現基因之分析 24 3.4.1 cDNA 微陣列雜合反應之分析 24 3.4.2 cDNA 微陣列雜合反應之分析 25 肆、 討論 26 伍、 參考資料 28 | |
dc.language.iso | zh-TW | |
dc.title | 比較橈足類與豐年蝦餵食點帶石斑魚苗之發生與基因表現 | zh_TW |
dc.title | Comparison of gene expression profile and development of red spotted grouper (Epinephelus coioides) larvae fed with copepod and Artemia | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 張繼堯(Chi-Yao Chang) | |
dc.contributor.oralexamcommittee | 林正輝(Cheng-Hui Lin) | |
dc.subject.keyword | 石斑魚, 高度不飽和脂肪酸, | zh_TW |
dc.subject.keyword | grouper, DHA, EPA, | en |
dc.relation.page | 46 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2009-08-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 1.45 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。