請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22751完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊建志 | |
| dc.contributor.author | Ai-Ling Kao | en |
| dc.contributor.author | 高艾玲 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:26:52Z | - |
| dc.date.copyright | 2010-02-24 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-02-10 | |
| dc.identifier.citation | Alonso, J. M., Stepanova, A. N., Leisse, T. J., Kim, C. J., Chen, H., Shinn, P., Stevenson, D. K., Zimmerman, J., Barajas, P., Cheuk, R., Gadrinab, C., Heller, C., Jeske, A., Koesema, E., Meyers, C. C., Parker, H., Prednis, L., Ansari, Y., Choy, N., Deen, H., Geralt, M., Hazari, N., Hom, E., Karnes, M., Mulholland, C., Ndubaku, R., Schmidt, I., Guzman, P., Aguilar-Henonin, L., Schmid, M., Weigel, D., Carter, D. E., Marchand, T., Risseeuw, E., Brogden, D., Zeko, A., Crosby, W. L., Berry, C. C. & Ecker, J. R. (2003). Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633): 653-657.
Anzi, C., Pelucchi, P., Vazzola, V., Murgia, I., Gomarasca, S., Piccoli, M. B. &Morandini, P. (2008). The proton pump interactor (Ppi) gene family of Arabidopsis thaliana: expression pattern of Ppi1 and characterisation of knockout mutants for Ppi1 and 2. Plant Biol (Stuttg) 10(2): 237-249. Baba, K., Nakano, T., Yamagishi, K. &Yoshida, S. (2001). Involvement of a nuclear-encoded basic helix-loop-helix protein in transcription of the light-responsive promoter of psbD. Plant Physiol 125(2): 595-603. Bartalesi, I., Bertini, I., Ghosh, K., Rosato, A. &Turano, P. (2002). The unfolding of oxidized c-type cytochromes: the instructive case of Bacillus pasteurii. J Mol Biol 321(4): 693-701. Berry, E. A. &Trumpower, B. L. (1987). Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra. Anal Biochem 161(1): 1-15. Bhat, R. A., Lahaye, T. &Panstruga, R. (2006). The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods. Plant Methods 2: 12. Bonza, M. C., Fusca, T., Homann, U., Thiel, G. &De Michelis, M. I. (2009). Intracellular localisation of PPI1 (proton pump interactor, isoform 1), a regulatory protein of the plasma membrane H(+)-ATPase of Arabidopsis thaliana. Plant Biol (Stuttg) 11(6): 869-877. Boonyaratanakornkit, V. &Edwards, D. P. (2004). Receptor mechanisms of rapid extranuclear signalling initiated by steroid hormones. Essays Biochem 40: 105-120. Boonyaratanakornkit, V., Scott, M. P., Ribon, V., Sherman, L., Anderson, S. M., Maller, J. L., Miller, W. T. &Edwards, D. P. (2001). Progesterone receptor contains a proline-rich motif that directly interacts with SH3 domains and activates c-Src family tyrosine kinases. Mol Cell 8(2): 269-280. Boursiac, Y., Chen, S., Luu, D. T., Sorieul, M., van den Dries, N. &Maurel, C. (2005). Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression. Plant Physiol 139(2): 790-805. Boyes DC, Zayed AM, Ascenzi R, McCaskill AJ, Hoffman NE, Davis KR, Gorlach J. (2001) 'Growth stage-based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants.' Plant Cell 13(7): 1499-510. Chang S, Puryear J, Cairney J. (1993) 'A simple and efficient method for isolating RNA from pine tree.' Plant Molecular Biology Reporter 11: 113-116. Chomczynski P, and Sacchi N. (1987) 'Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.' Anal Biochem 162(1): 156-159. Clouse, S. D. &Sasse, J. M. (1998). BRASSINOSTEROIDS: Essential Regulators of Plant Growth and Development. Annu Rev Plant Physiol Plant Mol Biol 49: 427-451. Cooper AJ, Krasnikov BF, Okuno E, Jeitner TM. (2003) “L-alanine-glyoxylate aminotransferase II of rat kidney and liver mitochondria possesses cysteine S-conjugate beta-lyase activity:a contributing factor to the nephrotoxicity/hepatotoxicity of halogenated alkenes?” Biochem J. 376(Pt 1): 169-78. Craven, R. J., Mallory, J. C. &Hand, R. A. (2007). Regulation of iron homeostasis mediated by the heme-binding protein Dap1 (damage resistance protein 1) via the P450 protein Erg11/Cyp51. J Biol Chem 282(50): 36543-36551. Crudden, G., Chitti, R. E. &Craven, R. J. (2006). Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. J Pharmacol Exp Ther 316(1): 448-455. Crudden, G., Loesel, R. &Craven, R. J. (2005). Overexpression of the cytochrome p450 activator hpr6 (heme-1 domain protein/human progesterone receptor) in tumors. Tumour Biol 26(3): 142-146. Damkjaer, J. T., Kereiche, S., Johnson, M. P., Kovacs, L., Kiss, A. Z., Boekema, E. J., Ruban, A. V., Horton, P. &Jansson, S. (2009). The photosystem II light-harvesting protein Lhcb3 affects the macrostructure of photosystem II and the rate of state transitions in Arabidopsis. Plant Cell 21(10): 3245-3256. Danpure, C. J. (1997). Variable peroxisomal and mitochondrial targeting of alanine: glyoxylate aminotransferase in mammalian evolution and disease. Bioessays 19(4): 317-326. Dharmasiri, N., Dharmasiri, S. &Estelle, M. (2005). The F-box protein TIR1 is an auxin receptor. Nature 435(7041): 441-445. Di Fiore, P. P., Polo, S. &Hofmann, K. (2003). When ubiquitin meets ubiquitin receptors: a signalling connection. Nat Rev Mol Cell Biol 4(6): 491-497. Dioum, E. M., Rutter, J., Tuckerman, J. R., Gonzalez, G., Gilles-Gonzalez, M. A. &McKnight, S. L. (2002). NPAS2: a gas-responsive transcription factor. Science 298(5602): 2385-2387. Does, M. P., Dekker, B. M., de Groot, M. J. &Offringa, R. (1991). A quick method to estimate the T-DNA copy number in transgenic plants at an early stage after transformation, using inverse PCR. Plant Mol Biol 17(1): 151-153. Dudley, C. A., Cooper, K. J. &Moss, R. L. (1980). Progesterone-induced mating behavior in rats on the day following spontaneous heat. Physiol Behav 25(5): 759-762. Edwards, D. P. (2005). Regulation of signal transduction pathways by estrogen and progesterone. Annu Rev Physiol 67: 335-376. Falkenstein, E., Meyer, C., Eisen, C., Scriba, P. C. &Wehling, M. (1996). Full-length cDNA sequence of a progesterone membrane-binding protein from porcine vascular smooth muscle cells. Biochem Biophys Res Commun 229(1): 86-89. Falkenstein, E. &Wehling, M. (2000). Nongenomically initiated steroid actions. Eur J Clin Invest 30 Suppl 3: 51-54. Furuyama, K., Kaneko, K. &Vargas, P. D. (2007). Heme as a magnificent molecule with multiple missions: heme determines its own fate and governs cellular homeostasis. Tohoku J Exp Med 213(1): 1-16. Gerdes, D., Wehling, M., Leube, B. &Falkenstein, E. (1998). Cloning and tissue expression of two putative steroid membrane receptors. Biol Chem 379(7): 907-911. Gfeller, A., Liechti, R. &Farmer, E. E. (2006). Arabidopsis jasmonate signaling pathway. Sci STKE 2006(322): cm1. Ghosh, K., Thompson, A. M., Goldbeck, R. A., Shi, X., Whitman, S., Oh, E., Zhiwu, Z., Vulpe, C. &Holman, T. R. (2005). Spectroscopic and biochemical characterization of heme binding to yeast Dap1p and mouse PGRMC1p. Biochemistry 44(50): 16729-16736. Hanano, A., Burcklen, M., Flenet, M., Ivancich, A., Louwagie, M., Garin, J. &Blee, E. (2006). Plant seed peroxygenase is an original heme-oxygenase with an EF-hand calcium binding motif. J Biol Chem 281(44): 33140-33151. Hand, R. A. &Craven, R. J. (2003). Hpr6.6 protein mediates cell death from oxidative damage in MCF-7 human breast cancer cells. J Cell Biochem 90(3): 534-547. Hand, R. A., Jia, N., Bard, M. &Craven, R. J. (2003). Saccharomyces cerevisiae Dap1p, a novel DNA damage response protein related to the mammalian membrane-associated progesterone receptor. Eukaryot Cell 2(2): 306-317. Haubrick, L. L. &Assmann, S. M. (2006). Brassinosteroids and plant function: some clues, more puzzles. Plant Cell Environ 29(3): 446-457. He, X. J., Mu, R. L., Cao, W. H., Zhang, Z. G., Zhang, J. S. &Chen, S. Y. (2005). AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. Plant J 44(6): 903-916. Hershko, A. &Ciechanover, A. (1998). The ubiquitin system. Annu Rev Biochem 67: 425-479. Hershko, A., Ciechanover, A. &Varshavsky, A. (2000). Basic Medical Research Award. The ubiquitin system. Nat Med 6(10): 1073-1081. Hicke, L., Schubert, H. L. &Hill, C. P. (2005). Ubiquitin-binding domains. Nat Rev Mol Cell Biol 6(8): 610-621. Hoeller, D., Hecker, C. M., Wagner, S., Rogov, V., Dotsch, V. &Dikic, I. (2007). E3-independent monoubiquitination of ubiquitin-binding proteins. Mol Cell 26(6): 891-898. Hughes, A. L., Powell, D. W., Bard, M., Eckstein, J., Barbuch, R., Link, A. J. &Espenshade, P. J. (2007). Dap1/PGRMC1 binds and regulates cytochrome P450 enzymes. Cell Metab 5(2): 143-149. Ishikawa, H., Kato, M., Hori, H., Ishimori, K., Kirisako, T., Tokunaga, F. &Iwai, K. (2005). Involvement of heme regulatory motif in heme-mediated ubiquitination and degradation of IRP2. Mol Cell 19(2): 171-181. Itoh, H., Matsuoka, M. &Steber, C. M. (2003). A role for the ubiquitin-26S-proteasome pathway in gibberellin signaling. Trends Plant Sci 8(10): 492-497. Iwai, K., Drake, S. K., Wehr, N. B., Weissman, A. M., LaVaute, T., Minato, N., Klausner, R. D., Levine, R. L. &Rouault, T. A. (1998). Iron-dependent oxidation, ubiquitination, and degradation of iron regulatory protein 2: implications for degradation of oxidized proteins. Proc Natl Acad Sci U S A 95(9): 4924-4928. Kendziorek, M. &Paszkowski, A. (2008). Properties of serine:glyoxylate aminotransferase purified from Arabidopsis thaliana leaves. Acta Biochim Biophys Sin (Shanghai) 40(2): 102-110. Kim, S. Y., Kim, S. G., Kim, Y. S., Seo, P. J., Bae, M., Yoon, H. K. &Park, C. M. (2007). Exploring membrane-associated NAC transcription factors in Arabidopsis: implications for membrane biology in genome regulation. Nucleic Acids Res 35(1): 203-213. Kim, Y. S., Kim, S. G., Park, J. E., Park, H. Y., Lim, M. H., Chua, N. H. &Park, C. M. (2006). A membrane-bound NAC transcription factor regulates cell division in Arabidopsis. Plant Cell 18(11): 3132-3144. Kraft, E., Stone, S. L., Ma, L., Su, N., Gao, Y., Lau, O. S., Deng, X. W. &Callis, J. (2005). Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis. Plant Physiol 139(4): 1597-1611. Krebs, C. J., Jarvis, E. D., Chan, J., Lydon, J. P., Ogawa, S. &Pfaff, D. W. (2000). A membrane-associated progesterone-binding protein, 25-Dx, is regulated by progesterone in brain regions involved in female reproductive behaviors. Proc Natl Acad Sci U S A 97(23): 12816-12821. Kurita, T., Lee, K., Saunders, P. T., Cooke, P. S., Taylor, J. A., Lubahn, D. B., Zhao, C., Makela, S., Gustafsson, J. A., Dahiya, R. &Cunha, G. R. (2001a). Regulation of progesterone receptors and decidualization in uterine stroma of the estrogen receptor-alpha knockout mouse. Biol Reprod 64(1): 272-283. Kurita, T., Wang, Y. Z., Donjacour, A. A., Zhao, C., Lydon, J. P., O'Malley, B. W., Isaacs, J. T., Dahiya, R. &Cunha, G. R. (2001b). Paracrine regulation of apoptosis by steroid hormones in the male and female reproductive system. Cell Death Differ 8(2): 192-200. Lambert, J. J., Belelli, D., Peden, D. R., Vardy, A. W. &Peters, J. A. (2003). Neurosteroid modulation of GABAA receptors. Prog Neurobiol 71(1): 67-80. Lathrop, J. T. &Timko, M. P. (1993). Regulation by heme of mitochondrial protein transport through a conserved amino acid motif. Science 259(5094): 522-525. Leonhardt, S. A., Boonyaratanakornkit, V. &Edwards, D. P. (2003). Progesterone receptor transcription and non-transcription signaling mechanisms. Steroids 68(10-13): 761-770. Li, J., Nam, K. H., Vafeados, D. &Chory, J. (2001). BIN2, a new brassinosteroid-insensitive locus in Arabidopsis. Plant Physiol 127(1): 14-22. Liechti, R., Gfeller, A. &Farmer, E. E. (2006). Jasmonate signaling pathway. Sci STKE 2006(322): cm2. Liepman, A. H. &Olsen, L. J. (2001). Peroxisomal alanine : glyoxylate aminotransferase (AGT1) is a photorespiratory enzyme with multiple substrates in Arabidopsis thaliana. Plant J 25(5): 487-498. Liepman, A. H. &Olsen, L. J. (2003). Alanine aminotransferase homologs catalyze the glutamate:glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis. Plant Physiol 131(1): 215-227. Lim-Tio, S. S., Keightley, M. C. &Fuller, P. J. (1997). Determinants of specificity of transactivation by the mineralocorticoid or glucocorticoid receptor. Endocrinology 138(6): 2537-2543. Losel, R. M., Falkenstein, E., Feuring, M., Schultz, A., Tillmann, H. C., Rossol-Haseroth, K. &Wehling, M. (2003). Nongenomic steroid action: controversies, questions, and answers. Physiol Rev 83(3): 965-1016. Luciano, A. M., Pappalardo, A., Ray, C. &Peluso, J. J. (1994). Epidermal growth factor inhibits large granulosa cell apoptosis by stimulating progesterone synthesis and regulating the distribution of intracellular free calcium. Biol Reprod 51(4): 646-654. Mahesh, V. B. &Muldoon, T. G. (1987). Integration of the effects of estradiol and progesterone in the modulation of gonadotropin secretion. J Steroid Biochem 27(4-6): 665-675. Mallory, J. C., Crudden, G., Johnson, B. L., Mo, C., Pierson, C. A., Bard, M. &Craven, R. J. (2005). Dap1p, a heme-binding protein that regulates the cytochrome P450 protein Erg11p/Cyp51p in Saccharomyces cerevisiae. Mol Cell Biol 25(5): 1669-1679. Mense, S. M. &Zhang, L. (2006). Heme: a versatile signaling molecule controlling the activities of diverse regulators ranging from transcription factors to MAP kinases. Cell Res 16(8): 681-692. Meyer, C., Schmid, R., Schmieding, K., Falkenstein, E. &Wehling, M. (1998a). Characterization of high affinity progesterone-binding membrane proteins by anti-peptide antiserum. Steroids 63(2): 111-116. Meyer, C., Schmid, R., Scriba, P. C. &Wehling, M. (1996). Purification and partial sequencing of high-affinity progesterone-binding site(s) from porcine liver membranes. Eur J Biochem 239(3): 726-731. Meyer, C., Schmieding, K., Falkenstein, E. &Wehling, M. (1998b). Are high-affinity progesterone binding site(s) from porcine liver microsomes members of the sigma receptor family? Eur J Pharmacol 347(2-3): 293-299. Mifsud, W. &Bateman, A. (2002). Membrane-bound progesterone receptors contain a cytochrome b5-like ligand-binding domain. Genome Biol 3(12): RESEARCH0068. Min, L., Strushkevich, N. V., Harnastai, I. N., Iwamoto, H., Gilep, A. A., Takemori, H., Usanov, S. A., Nonaka, Y., Hori, H., Vinson, G. P. &Okamoto, M. (2005). Molecular identification of adrenal inner zone antigen as a heme-binding protein. Febs J 272(22): 5832-5843. Min, L., Takemori, H., Nonaka, Y., Katoh, Y., Doi, J., Horike, N., Osamu, H., Raza, F. S., Vinson, G. P. &Okamoto, M. (2004). Characterization of the adrenal-specific antigen IZA (inner zone antigen) and its role in the steroidogenesis. Mol Cell Endocrinol 215(1-2): 143-148. Morey, A. K., Pedram, A., Razandi, M., Prins, B. A., Hu, R. M., Biesiada, E. &Levin, E. R. (1997). Estrogen and progesterone inhibit vascular smooth muscle proliferation. Endocrinology 138(8): 3330-3339. Morishita, T., Kojima, Y., Maruta, T., Nishizawa-Yokoi, A., Yabuta, Y. &Shigeoka, S. (2009). Arabidopsis NAC transcription factor, ANAC078, regulates flavonoid biosynthesis under high-light. Plant Cell Physiol 50(12): 2210-2222. Morris, J. R. &Solomon, E. (2004). BRCA1 : BARD1 induces the formation of conjugated ubiquitin structures, dependent on K6 of ubiquitin, in cells during DNA replication and repair. Hum Mol Genet 13(8): 807-817. Muldoon, T. G. &Mahesh, V. B. (1987). Receptor-weighted mechanistic approach to analysis of the actions of estrogen and progesterone on gonadotropin secretion. Adv Exp Med Biol 219: 47-64. Mussig, C. &Altmann, T. (2001). Brassinosteroid signaling in plants. Trends Endocrinol Metab 12(9): 398-402. Nordeen, S. K., Moyer, M. L. &Bona, B. J. (1994). The coupling of multiple signal transduction pathways with steroid response mechanisms. Endocrinology 134(4): 1723-1732. Ogawa, K., Sun, J., Taketani, S., Nakajima, O., Nishitani, C., Sassa, S., Hayashi, N., Yamamoto, M., Shibahara, S., Fujita, H. &Igarashi, K. (2001). Heme mediates derepression of Maf recognition element through direct binding to transcription repressor Bach1. EMBO J 20(11): 2835-2843. Peltier, J. B., Ytterberg, A. J., Sun, Q. &van Wijk, K. J. (2004). New functions of the thylakoid membrane proteome of Arabidopsis thaliana revealed by a simple, fast, and versatile fractionation strategy. J Biol Chem 279(47): 49367-49383. Peluso, J. J. (2006). Multiplicity of progesterone's actions and receptors in the mammalian ovary. Biol Reprod 75(1): 2-8. Peluso, J. J. &Pappalardo, A. (2004). Progesterone regulates granulosa cell viability through a protein kinase G-dependent mechanism that may involve 14-3-3sigma. Biol Reprod 71(6): 1870-1878. Peluso, J. J., Pappalardo, A., Losel, R. &Wehling, M. (2005). Expression and function of PAIRBP1 within gonadotropin-primed immature rat ovaries: PAIRBP1 regulation of granulosa and luteal cell viability. Biol Reprod 73(2): 261-270. Peluso, J. J., Pappalardo, A., Losel, R. &Wehling, M. (2006). Progesterone membrane receptor component 1 expression in the immature rat ovary and its role in mediating progesterone's antiapoptotic action. Endocrinology 147(6): 3133-3140. Petersen, N. H., Joensen, J., McKinney, L. V., Brodersen, P., Petersen, M., Hofius, D. &Mundy, J. (2009). Identification of proteins interacting with Arabidopsis ACD11. J Plant Physiol 166(6): 661-666. Petersen, N. H., McKinney, L. V., Pike, H., Hofius, D., Zakaria, A., Brodersen, P., Petersen, M., Brown, R. E. &Mundy, J. (2008). Human GLTP and mutant forms of ACD11 suppress cell death in the Arabidopsis acd11 mutant. FEBS J 275(17): 4378-4388. Pickart, C. M. &Eddins, M. J. (2004). Ubiquitin: structures, functions, mechanisms. Biochim Biophys Acta 1695(1-3): 55-72. Polo, S., Sigismund, S., Faretta, M., Guidi, M., Capua, M. R., Bossi, G., Chen, H., De Camilli, P. &Di Fiore, P. P. (2002). A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins. Nature 416(6879): 451-455. Qi, Z., Hamza, I. &O'Brian, M. R. (1999). Heme is an effector molecule for iron-dependent degradation of the bacterial iron response regulator (Irr) protein. Proc Natl Acad Sci U S A 96(23): 13056-13061. Qi, Z. &O'Brian, M. R. (2002). Interaction between the bacterial iron response regulator and ferrochelatase mediates genetic control of heme biosynthesis. Mol Cell 9(1): 155-162. Reddy, D. S., Castaneda, D. C., O'Malley, B. W. &Rogawski, M. A. (2004). Anticonvulsant activity of progesterone and neurosteroids in progesterone receptor knockout mice. J Pharmacol Exp Ther 310(1): 230-239. Rohe, H. J., Ahmed, I. S., Twist, K. E. &Craven, R. J. (2009). PGRMC1 (progesterone receptor membrane component 1): a targetable protein with multiple functions in steroid signaling, P450 activation and drug binding. Pharmacol Ther 121(1): 14-19. Ruban, A. V., Solovieva, S., Lee, P. J., Ilioaia, C., Wentworth, M., Ganeteg, U., Klimmek, F., Chow, W. S., Anderson, J. M., Jansson, S. &Horton, P. (2006). Plasticity in the composition of the light harvesting antenna of higher plants preserves structural integrity and biological function. J Biol Chem 281(21): 14981-14990. Sakamoto, H., Ukena, K., Takemori, H., Okamoto, M., Kawata, M. &Tsutsui, K. (2004). Expression and localization of 25-Dx, a membrane-associated putative progesterone-binding protein, in the developing Purkinje cell. Neuroscience 126(2): 325-334. Santi, S. &Schmidt, W. (2009). Dissecting iron deficiency-induced proton extrusion in Arabidopsis roots. New Phytol 183(4): 1072-1084. Schmid, M., Davison, T. S., Henz, S. R., Pape, U. J., Demar, M., Vingron, M., Scholkopf, B., Weigel, D. &Lohmann, J. U. (2005). A gene expression map of Arabidopsis thaliana development. Nat Genet 37(5): 501-506. Selmin, O., Lucier, G. W., Clark, G. C., Tritscher, A. M., Vanden Heuvel, J. P., Gastel, J. A., Walker, N. J., Sutter, T. R. &Bell, D. A. (1996). Isolation and characterization of a novel gene induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rat liver. Carcinogenesis 17(12): 2609-2615. Siironen, P., Nordling, S., Louhimo, J., Haapiainen, R. &Haglund, C. (2005). Immunohistochemical expression of Bcl-2, Ki-67, and p21 in patients with papillary thyroid cancer. Tumour Biol 26(1): 50-56. Simoncini, T., Mannella, P., Fornari, L., Caruso, A., Varone, G. &Genazzani, A. R. (2003). In vitro effects of progesterone and progestins on vascular cells. Steroids 68(10-13): 831-836. Song, J., Vinarov, D., Tyler, E. M., Shahan, M. N., Tyler, R. C. &Markley, J. L. (2004). Hypothetical protein At2g24940.1 from Arabidopsis thaliana has a cytochrome b5 like fold. J Biomol NMR 30(2): 215-218. Song, L., Shi, Q. M., Yang, X. H., Xu, Z. H. &Xue, H. W. (2009). Membrane steroid-binding protein 1 (MSBP1) negatively regulates brassinosteroid signaling by enhancing the endocytosis of BAK1. Cell Res 19(7): 864-876. Stone, S. L., Hauksdottir, H., Troy, A., Herschleb, J., Kraft, E. &Callis, J. (2005). Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis. Plant Physiol 137(1): 13-30. Suzuki, H., Tashiro, S., Hira, S., Sun, J., Yamazaki, C., Zenke, Y., Ikeda-Saito, M., Yoshida, M. &Igarashi, K. (2004). Heme regulates gene expression by triggering Crm1-dependent nuclear export of Bach1. EMBO J 23(13): 2544-2553. Takahashi, T., Gasch, A., Nishizawa, N. &Chua, N. H. (1995). The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation. Genes Dev 9(1): 97-107. Taler, D., Galperin, M., Benjamin, I., Cohen, Y. &Kenigsbuch, D. (2004). Plant eR genes that encode photorespiratory enzymes confer resistance against disease. Plant Cell 16(1): 172-184. Thomas, P. E., Ryan, D. &Levin, W. (1976). An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels. Anal Biochem 75(1): 168-176. Thompson, A. M., Reddi, A. R., Shi, X., Goldbeck, R. A., Moenne-Loccoz, P., Gibney, B. R. &Holman, T. R. (2007). Measurement of the heme affinity for yeast dap1p, and its importance in cellular function. Biochemistry 46(50): 14629-14637. Tong, Y. &Guo, M. (2007). Cloning and characterization of a novel periplasmic heme-transport protein from the human pathogen Pseudomonas aeruginosa. J Biol Inorg Chem 12(6): 735-750. Torii, K. &Ogura, Y. (1968). Studies on the EPR and light absorption spectra of horse erythrocyte catalase and its derivatives. J Biochem 64(2): 171-179. Viero, C., Mechaly, I., Aptel, H., Puech, S., Valmier, J., Bancel, F. &Dayanithi, G. (2006). Rapid inhibition of Ca2+ influx by neurosteroids in murine embryonic sensory neurones. Cell Calcium 40(4): 383-391. Viotti, C., Luoni, L., Morandini, P. &De Michelis, M. I. (2005). Characterization of the interaction between the plasma membrane H-ATPase of Arabidopsis thaliana and a novel interactor (PPI1). FEBS J 272(22): 5864-5871. Wehling, M. (1997). Specific, nongenomic actions of steroid hormones. Annu Rev Physiol 59: 365-393. Weig, A., Deswarte, C. &Chrispeels, M. J. (1997). The major intrinsic protein family of Arabidopsis has 23 members that form three distinct groups with functional aquaporins in each group. Plant Physiol 114(4): 1347-1357. Weigel, D. &Glazebrook, J. (2002). ARABIDOPSIS - A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press. Woelk, T., Oldrini, B., Maspero, E., Confalonieri, S., Cavallaro, E., Di Fiore, P. P. &Polo, S. (2006). Molecular mechanisms of coupled monoubiquitination. Nat Cell Biol 8(11): 1246-1254. Yamanaka, K., Ishikawa, H., Megumi, Y., Tokunaga, F., Kanie, M., Rouault, T. A., Morishima, I., Minato, N., Ishimori, K. &Iwai, K. (2003). Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2. Nat Cell Biol 5(4): 336-340. Yang, J., Ishimori, K. &O'Brian, M. R. (2005a). Two heme binding sites are involved in the regulated degradation of the bacterial iron response regulator (Irr) protein. J Biol Chem 280(9): 7671-7676. Yang, X., Song, L. &Xue, H. W. (2008). Membrane steroid binding protein 1 (MSBP1) stimulates tropism by regulating vesicle trafficking and auxin redistribution. Mol Plant 1(6): 1077-1087. Yang, X. H., Xu, Z. H. &Xue, H. W. (2005). Arabidopsis membrane steroid binding protein 1 is involved in inhibition of cell elongation. Plant Cell 17(1): 116-131. Yoshitani, N., Satou, K., Saito, K., Suzuki, S., Hatanaka, H., Seki, M., Shinozaki, K., Hirota, H. &Yokoyama, S. (2005). A structure-based strategy for discovery of small ligands binding to functionally unknown proteins: combination of in silico screening and surface plasmon resonance measurements. Proteomics 5(6): 1472-1480. Zeng, G. (1998). Sticky-end PCR: new method for subcloning. Biotechniques 25(2): 206-208. Zenke-Kawasaki, Y., Dohi, Y., Katoh, Y., Ikura, T., Ikura, M., Asahara, T., Tokunaga, F., Iwai, K. &Igarashi, K. (2007). Heme induces ubiquitination and degradation of the transcription factor Bach1. Mol Cell Biol 27(19): 6962-6971. Zhu, Y., Bond, J. &Thomas, P. (2003a). Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor. Proc Natl Acad Sci U S A 100(5): 2237-2242. Zhu, Y., Rice, C. D., Pang, Y., Pace, M. &Thomas, P. (2003b). Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes. Proc Natl Acad Sci U S A 100(5): 2231-2236. Zimmermann, P., Hirsch-Hoffmann, M., Hennig, L. &Gruissem, W. (2004). GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol 136(1): 2621-2632. 林盈涵 (2009). 阿拉伯芥中AtMAPR家族蛋白質與其潛在自身泛素化修飾功能研究. 碩士論文 國立台灣大學微生物與生化學研究所. 范凱亭 (2005). 阿拉伯芥AtMAPRs之組織及細胞內定位探討. 碩士論文 國立台灣大學農業化學研究所. 高艾玲 (2002). 阿拉伯芥中MAPR同源蛋白質之選殖、表現與功能分析. 碩士論文 國立台灣大學農業化學研究所. 莊榮輝 (1985) 水稻蔗糖合成酶之研究. 博士論文 國立台灣大學農業化學研究所. 張碩修 (2003). 阿拉伯芥中與 AtMAPR 有交互作用的蛋白質篩選. 碩士論文 國立台灣大學農業化學研究所. 黃勻彥 (2008). 阿拉伯芥泛素化分析方法之建立與非生物性逆境相關 RING-型泛素黏合酶選殖. 碩士論文 國立台灣大學微生物與生化學研究所. 葉尚烜 (2006). 阿拉伯芥中AtMAPRs 基因靜默轉殖植株之建構與性狀分析. 碩士論文 國立台灣大學微生物與生化學研究所. 蔡佩璇 (2004). 阿拉伯芥中AtMAPRs基因靜默載體建構與表現分析. 碩士論文 國立台灣大學微生物與生化學研究所. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22751 | - |
| dc.description.abstract | 阿拉伯芥中發現四個與豬的MAPR (membrane associated progesterone receptor conponent 1) 有30~40% 相似度的同源性蛋白質，AtMAPR2、AtMAPR3、AtMAPR4與AtMAPR5。研究指出AtMAPR5 (putative membrane steroid binding protein, MSBP1) 位在細胞膜，藉由促進brassinosteroid (BR) 受體走向內噬作用途徑，負向調控其訊息傳導 (Song et al. 2009)。但對於其他AtMAPRs蛋白質的功能仍然缺乏了解。於大腸桿菌中表現AtMAPRs，並分析其蛋白質特性。AtMAPRs之UV-visible光譜顯示AtMAPRs具有血基質蛋白的特性，綜合過去研究，血基質結合能力似乎是MAPRs蛋白質家族的特徵之一。進一步利用EPR光譜研究AtMAPR2與血基質結合模式，結果顯示AtMAPR2與血基質為高自旋度五配位共價結合形式，並利用定位點突變方式找到兩個影響AtMAPR血基質親和力的重要殘基Tyr44與Tyr92。
之前研究發現AtMAPR3與AtMAPR5在胞外具有E3-independent自身泛素化修飾能力，在本論文中，證實AtMAPR2及AtMAPR4在E3不存在的情況下，也可以進行自身泛素化修飾，且其修飾方式為接上一個泛素。序列比對結果顯示AtMAPRs上具有類似UIM (ubiquitini-interacting motif，為泛素結合區塊UBD的一種) 的序列，其位於血基質結合區塊上。血基質的添加會降低AtMAPR2的自身泛素化修飾反應，血基質結合與泛素化修飾作用有競爭的現象，可能由於這兩種修飾作用都發生在AtMAPR2疏水性口袋結構上。即時定量聚合酶鏈鎖反應顯示AtMAPR2的基因表現會受brassinosteroid的刺激而下降，且AtMAPR2基因表現下降的RNAi植株對brassinosteroid的刺激較不敏感，以上結果顯示AtMAPR2具有血基質結合能力且可能參與brassinosteroid訊息傳導或生合成途徑。 以酵母菌雙雜合法找到AtMAPR3的交互作用蛋白AGT1 (alanine glyoxylate transaminase)，AGT1為參與光呼吸作用的重要酵素，本研究藉由GST pull-down與BiFC (bimolecular fluorescence complementation) 分析驗證兩者交互作用的真實性及專一性，且發現血基質結合區塊對其交互作用的發生扮演重要的角色。在AtMAPR3::GUS突變株GUS活性分析中顯示，AtMAPR3的基因表現會受到光照的誘導。不論在洋蔥表皮或阿拉伯芥原生質體的細胞內定位實驗指出，AtMAPR3會位在細胞膜上與囊狀構造 (vesicles) 中。AtMAPR3基因靜默突變株對過氧化氫的處理較為敏感，且在鹽逆境下種子發芽率較野生型高，AtMAPR3基因表現會受到與植物防禦機制相關的植物荷爾蒙 (茉莉酸與水楊酸) 及鹽逆境刺激而增加。綜合以上結果，AtMAPR3可能藉由與AGT1的交互作用而參與光呼吸作用的調控機制。 AtMAPR4基因靜默突變株與基因表現下降之突變株，在強光培養下呈現淡綠色葉子與開花時間較晚之性狀改變，以酵母菌雙雜合法篩選到AtMAPR4的交互作用蛋白質，為屬於位在ER膜上的轉錄因子NTL11與細胞核孔蛋白WIP1，並進一步以BiFC驗證其交互作用之專一性。NTL11基因表現會受到高溫強光而誘導，調控flavonoids生合成基因的表現，促進花青素合成因應強光傷害。WIP1可調控RanGAP1結合上細胞核膜的過程，調控物質進出細胞核，再者AtMAPR4細胞內定位位在細胞核膜與細胞膜等膜系構造上。推測AtMAPR4在細胞內可能藉由泛素化修飾作用，在強光或高溫的情況下，參與NTL11由ER釋放至細胞核的過程，進一步調控flavinoids生合成基因表現。 | zh_TW |
| dc.description.abstract | Four AtMAPRs (AtMAPR2, AtMAPR3, AtMPAR4, and AtMAPR5) in Arabidopsis sharing 30-40% similarity with porcine MAPR (membrane associated progesterone receptor component 1) have been identified and investigated. AtMAPR5 (putative membrane steroid binding protein, MSBP1) is a membrane protein and proposed to negatively regulate brassinosteroid signaling by enhancing the endocytosis (Song et al. 2009). However, the functions of other AtMAPRs are still unclear. In this study, the recombinant AtMAPRs were purified from E.coli and their molecular properties were investigated. The UV-visible absorption spectra showed that AtMAPRs were hemoproteins. The heme binding affinity seems to be a common feature of MAPR family proteins in vitro. The EPR spectra of the purified proteins showed that AtMAPR2 bound a heme chromophore in high-spin, five-coordinated type. Site-directed mutagenesis indicated that two Tyr residues, Y44 and Y92, were important for anchoring heme into a hydrophobic pocket.
Previous study on AtMAPR3 and AtMAPR5 showed they had E3-independent autoubiquitination ability in vitro. In this study, AtMAPR2 and truncated AtMAPR4 also possessed monoubiquitination ability without E3 ligase in vitro. The putative UIM (ubiquitin-interacting motif, a member of UBDs) was located in conserved heme/steroid binding domain of AtMAPRs. The autoubiquitination ability of AtMAPR2 seems to be decreased by adding heme in vitro. Heme binding might compete with the ubiquitin modification owing to their binding to the same hydrophobic pocket in AtMAPRs. The expression of AtMAPR2 was down-regulated by brassinosteroid as revealed by the real-time PCR analysis and the knockdown mutant of AtMAPR2 showed less sensitivity to brassinosteroid. These results indicated that AtMAPR2 had heme binding affinity in vitro and might be involved in brassinosteroid signaling or synthesis pathway. By GST pull-down and bimolecular fluorescence complementation (BiFC) assay, AtMAPR3 showed protein-protein interaction with alanine glyoxylate transaminase (AGT1) which participated in the plant photorespiration. The heme/steroid binding domain of AtMAPR3 played an important role in the interaction between AtMAPR3 and AGT1. In AtMAPR3::GUS plants, the GUS activity was up-regulated by light. AtMAPR3-GFP was located in plasma membrane and vesicles in onion epidermal cells or Arabidopsis protoplasts. The AtMAPR3 knockout mutant was more sensitive to H2O2 in 16-day-old seedlings and more resistant to salt stress by seed germination bioassay. The gene expression of AtMAPR3 was increased by treatments with defense-related hormones, jasmonate and salicylic acid, and NaCl. These results revealed that AtMAPR3 might be involved in the regulation on photorespiration by interacting with AGT1. The knockout and knockdown mutants of AtMAPR4 showed pale green leaves under strong light intensity and longer flowering time. Two interacting protein candidates of AtMAPR4, NTL11 (NTM1-like protein) and WIP1 (WPP-domain interacting protein), were found using yeast two hybrid experiments. The specific interaction between AtMAPR4 and NTL11 was confirmed by BiFC assay. NTL11 is involved in flavonoids biosynthesis. These results showed that AtMAPR4 might regulate flavonoids synthesis by interacting with NTL11. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:26:52Z (GMT). No. of bitstreams: 1 ntu-99-D92b47202-1.pdf: 11389346 bytes, checksum: 9ed6ac6a2c8c4e968d9646521ca54fb7 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 目錄 I
縮寫表 VIII 摘要 XI Abstract XIII 第一章 緒論 1 1.1類固醇類荷爾蒙之重要性及作用模式 1 1.1.1類固醇類荷爾蒙的作用模式 2 1.1.2基因體調控模式 (Genomic action) 及非基因體調控模式 (Non- genomic action) 2 1.2黃體激素及其作用模式 3 1.3黃體激素受體及訊息傳導模式 5 1.3.1 Nuclear Progesterone Receptor -- PR-A及PR-B 5 1.3.2 Membrane Progestin Receptor --mPRα、mPRβ及mPRγ 5 1.3.3 Membrane Associated Progesterone Receptor component 1 – MAPR (PGRMC1) 6 1.4 MAPR 家族 8 1.4.1 Cytochrome b5 like Heme/Steroid binding domain 8 1.4.2 MAPR家族成員 8 1.4.3 MAPR家族可能的受質 (Ligands) 10 1.4.3.1黃體激素/固醇類 10 1.4.3.2血基質 (heme) 11 1.5模式植物：阿拉伯芥 (Arabidopsis thaliana) 13 1.6 AtMAPRs家族蛋白質 15 1.6.1 AtMAPR2之NMR結構 17 1.6.2 AtMAPR5/MSBP1之功能 20 1.7研究方向及重點 21 第二章 材料與方法 22 2.1實驗材料 22 2.1.1植物材料 22 2.1.2載體 (vectors) 22 2.1.3菌種 24 2.2實驗藥品 24 2.2.1一般化學藥劑 24 2.2.2酵素 (限制酶及核酸修飾酶) 25 2.2.3培養基 25 2.3儀器設備 26 2.4實驗方法 27 2.4.1阿拉伯芥種植 27 2.4.1.1培養基之配製 27 2.4.1.2種子之表面消毒及低溫處理 29 2.4.1.3種子之無菌培養 29 2.4.1.4種子之土壤培養 29 2.4.1.5種子之收集 29 2.4.2 DNA之抽取與分析 30 2.4.2.1阿拉伯芥染色體DNA之抽取 (CTAB method) 30 2.4.2.2質體DNA之抽取 30 2.4.2.3 DNA限制酶切割之分析 32 2.4.2.4 DNA瓊脂糖膠體電泳 32 2.4.2.5 DNA片段之分離與純化 32 2.4.2.6 DNA之去磷酸化反應 33 2.4.2.7 DNA之磷酸化反應 33 2.4.2.8 DNA之定量 33 2.4.3 RNA之抽取與分析 33 2.4.3.1 Total RNA之抽取 (TRIzol reagent method) 33 2.4.3.2 Total RNA之抽取 (Pine tree method) 34 2.4.3.3 RNA濃度估算 34 2.4.3.4 DNase treatment 35 2.4.3.5 RT-PCR 36 2.4.4 AtMAPRs表現載體之建構 37 2.4.4.1表現重組蛋白質實驗之載體建構 37 2.4.4.2細胞、組織定位、BiFC及基因轉殖實驗之載體建構 40 2.4.4.3 AtMAPRs啟動子驅動GUS表現之載體建構 44 2.4.5重組蛋白質表現與檢定 44 2.4.5.1重組蛋白質表現條件之探討 44 2.4.5.2重組蛋白質之純化與分析 45 2.4.5.3蛋白質電泳檢定 46 2.4.6重組蛋白質血基質特性測定 50 2.4.6.1蛋白質UV-visible光譜測定 50 2.4.6.2 ICP-OES測定 50 2.4.6.3 EPR光譜測定 51 2.4.6.4 CD光譜測定 51 2.4.6.5血基質染色 51 2.4.7胞外泛素化修飾分析實驗 52 2.4.7.1胞外泛素化修飾反應 (in vitro ubiquitination assay) 52 2.4.7.2梯度電泳 (gradient SDS-PAGE) 52 2.4.8 AtMAPRs之T-DNA突變株鑑定與分析 53 2.4.8.1 T-DNA突變株同型結合子 (homozygote) 之確定 53 2.4.8.2單一T-DNA插入突變株 (single T-DNA insertional mutant) 之確定 54 2.4.8.3突變株中AtMAPRs基因表現之確認 56 2.4.9 AtMAPRs啟動子驅動GUS表現轉殖株之建立與GUS活性 分析……………………………………………………………..….58 2.4.9.1 AtMAPRs啟動子驅動GUS表現轉殖株之建立 58 2.4.9.2 GUS活性分析 59 2.4.10阿拉伯芥外表型 (phenotype) 之觀察 59 2.4.11 AtMAPRs於不同生長時期、不同器官與處理下的基因表現 60 2.4.11.1定量性即時聚合酶鏈鎖反應 (quantitative real-time PCR) 60 2.4.11.2阿拉伯芥不同生長時期、不同器官與處理之樣品收集 64 2.4.11.3各處理樣品之定量性即時聚合酶鏈鎖反應 64 2.4.12基因槍 (Particle bombardment) 轉殖及螢光顯微鏡觀察 65 2.4.12.1基因槍轉殖原理簡介 66 2.4.12.2植物材料之準備 68 2.4.12.3 DNA包裹鎢粒子之製備 68 2.4.12.4基因槍操作 68 2.4.12.5螢光顯微鏡觀察 69 2.4.12.6共軛焦螢光顯微鏡觀察 69 2.4.13阿拉伯芥原生質體轉殖及螢光顯微鏡觀察 70 2.4.13.1阿拉伯芥原生質體之製備 70 2.4.13.2 PEG轉形法 70 2.4.14 Glutathione S-transferase (GST) pull-down assay 71 2.4.14.1目標蛋白質GST-AtMAPR2及GST-AtMAPR5表現載體建構 71 2.4.14.2候選蛋白質AGT1-His6之表現載體建構 71 2.4.14.3目標與候選蛋白質之表現條件探討 71 2.4.14.4目標與候選蛋白質之pull down assay 72 2.4.15 AGT活性分析 73 2.4.15.1 GST-AtMAPR3與AGT1-His蛋白質之純化 73 2.4.15.2 AGT1-His之AGT活性分析 73 2.4.16 AtMAPR4單株抗體製備 74 2.4.16.1 AtMAPR4抗原製備 75 2.4.16.2小鼠尾巴採血 75 2.4.16.3抗原乳劑製備法 75 2.4.16.4小鼠免疫注射法 76 2.4.16.5細胞融合 76 2.4.16.6細胞保存法 78 2.4.16.7單株抗體生產 79 2.4.16.8免疫球蛋白之純化 80 2.4.16.9酵素免疫分析法 80 第三章 結果與討論 82 3.1 AtMAPRs具有結合血基質 (heme) 的特性 82 3.1.1 AtMAPRs重組蛋白質之UV-visible吸收光譜 82 3.1.2以ICP-OES檢測AtMAPRs重組蛋白質之heme含量及Soret band的消光係數 83 3.2 AtMAPR2與血基質結合模式探討 84 3.2.1 AtMAPR2電子順磁共振光譜 (Electron paramagnetic resonance spectra) 85 3.2.2以定位點突變方式探討AtMAPR2與heme結合的模式 85 3.2.2.1突變蛋白血基質結合能力測定 86 3.2.2.2突變蛋白之CD光譜測定 87 3.2.2.3雙突變蛋白血基質結合能力測定 87 3.2.2.4 AtMAPR2的原態蛋白及突變蛋白血基質染色 88 3.2.2.5 Y38、Y44及Y92對AtMAPR2血基質結合的重要影響 90 3.2.3血基質對AtMAPR2基因表現的影響 91 3.3 AtMAPRs具有E3-independent自身泛素化修飾 (autoubiqitination) 的活性 91 3.3.1 AtMAPR2具有胞外自身泛素化修飾活性 93 3.3.2 AtMAPR4具有胞外自身泛素化修飾活性 94 3.3.3 AtMAPRs自身泛素化修飾作用之討論 94 3.3.4血基質對AtMAPR2自身泛素化修飾的影響 95 3.4 AtMAPR2生理功能探討 97 3.4.1 AtMAPR2的細胞內定位 97 3.4.2 AtMAPR2基因表現情形分析 98 3.4.2.1 AtMAPR2於不同生長時期之表現情形 98 3.4.2.2 AtMAPR2於不同器官之表現情形 99 3.4.2.3 AtMAPR2於不同荷爾蒙處理下之表現情形 100 3.4.2.4 AtMAPR2於非生物性逆境下之表現情形 100 3.4.3 AtMAPR2突變株對brassinosteroid的反應 101 3.4.4 AtMAPR2可能扮演的生理功能 102 3.5 AtMAPR3生理功能探討 103 3.5.1 AtMAPR3相關T-DNA突變株之篩選 103 3.5.1.1 AtMAPR3之基因靜默突變株 103 3.5.1.2 AtMAPR3基因靜默突變株之外表型觀察 104 3.5.2 AtMAPR3基因表現情形分析 105 3.5.2.1 AtMAPR3於不同生長時期與不同器官之表現情形 105 3.5.2.2 AtMAPR2於不同荷爾蒙處理下之表現情形 106 3.5.2.3 AtMAPR3於非生物性逆境下之表現情形 107 3.5.3可能與AtMAPR3產生交互作用之蛋白質 107 3.5.3.1丙胺酸-乙醛酸轉胺酶 (alanine-glyoxylate aminotransferase 1，AGT1) 107 3.5.3.2泛素家族蛋白質 108 3.5.3.3跨膜蛋白質 (plasma membrane intrinsic protein, PIP3) 108 3.5.4 AtMAPR3與AGT1的蛋白質交互作用 109 3.5.4.1 AtMAPR3與AGT之GST pull down assay 109 3.5.4.2 AtMAPR3與AGT之BiFC assay 110 3.5.5 AtMAPR3的細胞內定位 111 3.5.6 AtMAPR3與AGT1作用關係的探討 111 3.5.6.1 AtMAPR3與對AGT1轉胺酶活性調控的探討 112 3.5.6.2 AtMAPR3對AGT1泛素化修飾作用調控之探討 112 3.5.6.3 AtMAPR3基因表現受光的調控且可能參與光呼吸作用 113 3.5.7 AtMAPR3基因靜默突變株對H2O2處裡較為敏感 113 3.5.8 AtMAPR3基因靜默突變株對NaCl處裡較為不敏感 114 3.5.9 AtMAPR3可能扮演的生理角色 114 3.6 AtMAPR4生理功能探討 115 3.6.1 AtMAPR4相關T-DNA突變株之篩選與性狀觀察 115 3.6.1.1 AtMAPR4基因靜默突變株之篩選 115 3.6.1.2突變株中AtMAPR4之基因表現偵測 116 3.6.1.3 AtMAPR4 突變株之表現型態 117 3.6.2 AtMAPR4之細胞內定位 117 3.6.3 AtMAPR4之基因表現情形 118 3.6.3.1 AtMAPR4於不同生長時期與不同器官之表現情形 118 3.6.3.2 AtMAPRA4於不同荷爾蒙處理下之表現情形 119 3.6.3.3 AtMAPR4於非生物性逆境下之表現情形 119 3.6.4可能與AtMAPR4產生交互作用之蛋白質 119 3.6.4.1 NAC家族蛋白質 120 3.6.4.2 WIP1 (WPP-domain Interacting Protein 1) 122 3.6.4.3 PPI1 (Proton Pump Interactor 1) 122 3.6.4.4 VAP27-1 (Vesicle Associated Protein 27-1) 122 3.6.4.5葉綠素A-B結合蛋白質 (Lhcb3) 123 3.6.4.6 TCP家族轉錄因子 (PTF1) 123 3.6.5 AtMAPR4與NTL11交互作用之檢驗 123 3.6.6 AtMAPR4與WIP1交互作用之檢驗 124 3.6.7 AtMAPR4 與冷、熱、強光刺激之探討 124 3.6.7.1 AtMAPR4與冷處理之探討 125 3.6.7.2 AtMAPR4與高溫逆境之探討 126 3.6.7.3 AtMAPR4與強光逆境之探討 126 3.6.8 AtMAPR4可能扮演的生理角色 127 3.6.9 AtMAPR4單株抗體之製備 127 3.6.9.1單株抗體之製備與多源抗體之取得 127 3.6.9.2抗體效價測定 128 3.6.9.3單株抗體專一性測定 128 第四章 結論未來展望 129 4.1結論…… 129 4.2 未來展望 130 4.2.1 AtMAPR2 NMR結構分析 130 4.2.2 AtMAPR2與AtMAPR4胞內血基質結合能力及泛素化飾修能力 測定 131 4.2.3 AtMAPRs突變株外表型的探討 131 4.2.4 T-DNA突變株之建立與篩選 132 4.2.5細胞定位之再確認 132 4.2.6可能與AtMAPRs產生交互作用蛋白質之探討 132 參考文獻 134 結果圖表集 144 附錄 206 | |
| dc.language.iso | zh-TW | |
| dc.subject | 血基質結合蛋白 | zh_TW |
| dc.subject | 黃酮類 | zh_TW |
| dc.subject | 光呼吸 | zh_TW |
| dc.subject | 自身泛素化修飾 | zh_TW |
| dc.subject | autoubiquitination | en |
| dc.subject | flavonoids | en |
| dc.subject | photorespiration | en |
| dc.subject | hemoprotein | en |
| dc.title | 阿拉伯芥 AtMAPRs 之分子特性研究 | zh_TW |
| dc.title | Molecular Characterization of AtMAPRs in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 蘇仲卿,莊榮輝,王愛玉,陳佩燁,俞聖法,陳炳宇 | |
| dc.subject.keyword | 血基質結合蛋白,自身泛素化修飾,光呼吸,黃酮類, | zh_TW |
| dc.subject.keyword | hemoprotein,autoubiquitination,photorespiration,flavonoids, | en |
| dc.relation.page | 221 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2010-02-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 未授權公開取用 | 11.12 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。