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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 王汎熒 | |
dc.contributor.author | Kuo-Chao Chiu | en |
dc.contributor.author | 邱國詔 | zh_TW |
dc.date.accessioned | 2021-06-08T04:15:55Z | - |
dc.date.copyright | 2010-08-16 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-04 | |
dc.identifier.citation | Auerbach, J., Prager, D., Neuhaus, S., Loss, U., Witte, K.H., 1994, Grouping of porcine enteroviruses by indirect immunofluorescence and description of two new serotypes. Zentralbl Veterinarmed B 41, 277-282.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22345 | - |
dc.description.abstract | 豬鐵士古病毒(PTV)為Picornaviridae科Teschovirus屬之無封套、大小約25-30 nm之單股正向RNA病毒。早期被歸類為Enterovirus,之後獨立為Teschovirus,並有至少11個血清型。鐵縣病( Teschen disease)最早於1929年於捷克的鐵縣鎮發現而命名之,為強毒株之PTV-1,在1930-1950年間造成豬的嚴重急性腦脊髓炎,導致歐洲養豬產業極大損失。1950年代之後,另一較弱毒性的病毒則在英國發現並造成Talfan disease (泰發病)。其後此弱毒株在世界廣為流行。台灣則在2000年首度發現並分離出PTV-1,之後不同型別的PTV在各地流行。本研究的目的為使用反轉錄-聚合酶鏈鎖反應(RT-PCR), 聚合酶鏈鎖反應(PCR), 巢式聚合酶鏈鎖反應(nested-PCR)結合免疫組織化學染色(IHC)及組織病理學檢查,研究PTVs感染在野外豬隻發病所扮演的角色。常見的病毒性疾病如豬瘟、假性狂犬病、日本腦炎亦使用PCR方法進行檢測。本研究蒐集了104隻淘汰之離乳豬,每隻豬隻的冷凍病材分成腦、淋巴組織、內臟組織及腸道四組進行PCR檢測。以頭數計PTVs之PCR陽性率為79.81%,而最常檢測到PTVs之部位為腸道(64.08%)及淋巴組織(45.63%),之後為腦(25.96%)及內臟組織(23.53%)。從屏東科技大學獸醫系病理診斷中心蒐集到57隻先前診斷為非化膿性腦炎的豬隻作為回溯性樣本,並使用IHC方法進行檢測。PTV-1抗原之分布於各臟器之情形為: 腦: 5.26%;扁桃腺: 15.79%;脾臟: 31.58%;淋巴結: 26.32%;腸系膜淋巴結: 22.81%;心臟: 0%;肝臟: 28.07%;腎臟:17.54%;胃: 0%;小腸: 26.32%;大腸: 24.56%。由PCR及IHC之結果得知,最常檢測到PTVs之部位為腸道及淋巴組織。在大多數非化膿性腦炎之病灶並無發現PTV-1病毒之抗原,但PTVs之感染可能對內臟、淋巴結及腸道既有的病變有誘發加重的效果。 | zh_TW |
dc.description.abstract | Porcine teschovirus (PTV) is a single-stranded, positive sense, spherical, nonenveloped RNA virus of the genus Teschovirus in the family Picornaviridae. PTVs were previously classified as Enterovirus genus, and were recently reclassified into at least 11 serotypes. Virulent PTV-1was first recognized as Teschen virus inducing polioencephalomyelitis in the Republic of Czechoslovakia in 1929. After year 1957, the lower virulent strain of PTV-1 occurred in the United Kindom called Talfan virus/disease. Nowadays, low virulent viruses spread all over the world. In Taiwan, PTV-1 was first isolated from pigs in year 2000, and the virus has since spread to most pig herds. The aim of this study was to analyze the role of PTVs infection in causing diseases in the fields by using reverse transcription polymerase chain reaction (RT-PCR) and Immunohistochemistry (IHC) combined with pathological examinations. Common swine viral infection like classical swine fever, pseudorabies, and Japanese encephalitis were also screened by PCR. Fresh frozen tissues of 104 culled postweaning pigs were collected. The tissues of each pig were pooled into four parts (groups) as brain, visceral organs, lymphoid organs, and the intestines. The positive rate of PTVs infection was 79.81% by heads, and most commonly detected in the intestines (64.08%) and lymphoid organs (45.63%), followed by the brain (25.96%) and visceral organs (23.53%). To analyze the role of PTV-1 infection in causing encephalitis, formalin-fixed paraffin-embeded (FFPE) archive tissues from 57 pigs formerly diagnosed as non-suppurative encephalitis were collected for IHC. The rates of PTV-1 antigen detection in each organ were brain: 5.26%, tonsil: 15.79%, spleen: 31.58%, lymph nodes: 26.32%, mesenteric lymph node: 22.81%, heart: 0%, liver: 28.07%, kidney: 17.54%, stomach: 0%, small intestines: 26.32%, large intestines: 24.56%. Both PCR and IHC showed that PTVs were most commonly detected in lymphoid organs and intestines. In most cases of encephalitis, there was no intralesional PTV-1 antigen detected. However, the presence of PTVs may have significance in aggravating pre-existed lesions in the visceras, lymphoid organs, and intestines, because these lesions were associated with higher PTVs detection. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:15:55Z (GMT). No. of bitstreams: 1 ntu-99-R97629015-1.pdf: 7581315 bytes, checksum: efcb30c7786612624b30f3cc4f29328f (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract II Contents IV List of figures VII List of Tables IX Chapter I INTRODUCTION 1 Chapter II LITERATURE REVIEW 4 2.1 Porcine Teschovirus 4 2.1.1 History 4 2.1.2 Taxonomy, Classification and Characteristics 5 2.1.3 Morphology, Genomic Organization and Gene Expression 6 2.1.4 Clinical signs 7 2.1.5 Lesions 11 2.1.6 Pathogenesis 11 2.1.7 Porcine teschovirus in Taiwan 13 2.1.8 Co-infection of Porcine teschovirus with other diseases 14 2.1.9 Vaccination of Porcine teschovirus 14 2.2 Polymerase chain reaction 15 2.3 Immunohistochemistry (IHC) 16 2.3.1 Types of antibodies 16 2.3.2 Antigen retrieval (AR) 17 2.3.3 Direct immunohistochemistry 18 2.3.4 Indirect immunohistochemistry 18 2.3.5 Modified Immunohistochemistry 19 Chapter III MATERIALS AND METHODS 21 3.1 Samples preparation 22 3.1.1 Fresh samples from field 22 3.1.2 FFPE archive samples from NPUST 23 3.2 Histopathological examination 24 3.3 Molecular analysis 24 3.3.1 RNA extraction 24 3.3.2 DNA extraction 25 3.3.3 Primer selection 26 3.3.4 Reverse transcription polymerase chain reaction (RT-PCR) and One-step RT-PCR 29 3.3.5 Polymerase chain reaction (PCR) and Nested-PCR amplification 30 3.3.6 Gel electrophoresis 31 3.4 Cloning and plasmid extraction 32 3.4.1 Cloning 32 3.4.2 Plasmid extraction 33 3.5 Immunohistochemistry 34 3.5.1 Antibodies 34 3.5.2 Classical indirect immunohistochemistry 35 3.5.3 Modified indirect immunohistochemistry 36 Chapter IV RESULTS 38 4.1 Sample collection 38 4.2 PCR assays 39 4.2.1 Results of G3PDH 39 4.2.2 Results of PCR and nested-PCR for PTV 39 4.2.3 Results of PCR and nested-PCR for CSFV 40 4.2.4 PCR Results for PrV 41 4.2.5 PCR Results for JEV 41 4.3 Gross pathological and histopathological examinations of culled postweaner pigs 41 4.4 Immunohistochemical examination of archive specimens 43 4.4.1 PTV-1 antigen in archive tissues 44 4.4.2 CSFV antigen in archive tissues 46 4.4.3 PrV antigen in archive tissues 47 4.4.4 JEV antigen in archive tissues 48 4.4.5 Summary of viral antigen detection in archive specimens of pigs at different growth stages 48 4.4.6 Co-infection or co-detection of PTV-1, CSFV and PrV antigen in archive tissues 49 Chapter V DISSCUSSION AND CONCLUSION 50 Chapter VI References 94 | |
dc.language.iso | en | |
dc.title | 由離乳後淘汰豬隻組織中偵測豬鐵士古病毒 | zh_TW |
dc.title | Detection of Porcine Teschovirus from Culled Postweaner Pigs | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡信雄,許天來,黃金城,李龍湖 | |
dc.subject.keyword | 豬;豬鐵士古病毒;組織病理學;聚合酶,鏈鎖反應;免疫組織化學染色, | zh_TW |
dc.subject.keyword | Pig; Porcine teschovirus;Histopathology;Polymerase chain reaction;Immunohistochemistry, | en |
dc.relation.page | 99 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2010-08-04 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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