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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 羅禮強 | |
| dc.contributor.author | Yi-Ling Hu | en |
| dc.contributor.author | 胡依伶 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:14:35Z | - |
| dc.date.copyright | 2010-08-18 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-10 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22263 | - |
| dc.description.abstract | 蛋白質SH2結構區在蛋白質-蛋白質相互作用上扮演關鍵的角色，而此類作用主要是透過SH2結構區與特定位置的磷酸化酪胺酸來進行。因此我們設計並合成出仿磷酸酪胺酸的單體分子，利用Fmoc 方法進行組合式固相胜肽合成，用以建構包含此仿磷酸酪胺酸單體的三胜肽和四胜肽分子庫，作為篩選蛋白質SH2結構區的抑制劑。其中，三胜肽是以Grb2 SH2結構區所對應的YXN序列，而四胜肽則是以STAT SH2結構區所對應的YXXQ序列來設計。此外，這些胜肽分子庫N 端上涵蓋有三種不同基團，以增加其分子庫的規模。 | zh_TW |
| dc.description.abstract | The Src-homology 2 (SH2) domains play an important role in protein-protein interaction, and are specific for the phosphotyrosine-containing peptides. Herein, we design and synthesis the phosphotyrosyl mimetic as a building block to set up the compound library using Fmoc solid phase peptide synthesis. And the library is composed of the phosphotyrosyl mimetic-containing tri- and tetrapeptides as SH2 domain inhibitors. In this library, the Grb2-SH2 domain preferably binds phosphotyrosyl peptides with the consensus sequence YXN, and the STAT-SH2 domain recognizes YXXQ tetrapeptides sequence. Additionally, in order to increase the scope of the library, we perform three different N-terminal modification in this peptides. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:14:35Z (GMT). No. of bitstreams: 1 ntu-99-R97223201-1.pdf: 7849430 bytes, checksum: a2d693f89dfff09bab0dd547e74eea53 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書.....i 誌謝.....ii 英文縮寫.....iv 胺基酸縮寫.....v 中文摘要.....vi 英文摘要.....viii 第一章 緒論.....1 1.1 Grb2和STAT蛋白質.....1 1.2 Grb2抑制劑之設計.....3 1.3 STAT抑制劑之設計.....5 1.4 固相胜肽合成.....6 1.5 Competition inhibition assays.....9 1.6 目標分子庫之設計.....10 第二章 結果與討論.....13 2.1 三胜肽和四胜肽分子庫之逆合成分析......13 2.2 仿磷酸酪胺酸單體之逆合成分析.....14 2.3 仿磷酸酪胺酸單體之合成方法.....15 2.3.1 化合物3的合成.....15 2.3.2 化合物4的合成.....17 2.3.3 化合物6的合成.....17 2.4 典型固相合成.....19 2.4.1 探討去保護試劑對於化合物7的影響.....22 2.4.2 去除Fmoc保護基.....22 2.4.3 胜肽鍵的耦合.....23 2.4.4側鏈保護基移除及胜肽脫離樹脂.....25 2.5 X-cpTyr-AA2-Asn-NH2三胜肽的合成......25 2.6 X-cpTyr-AA2-AA1-Gln四胜肽的合成......28 2.7 ELISA assay.....30 2.8 SH2天然受質的合成.....31 2.9三胜肽、四胜肽和SH2天然受質之數據分析.....32 2.10 結論.....37 第三章 實驗部分.....38 3.1一般敘述.....38 3.1.1測試及實驗儀器.....38 3.2 反應試劑.....39 3.3有機合成實驗步驟及光譜數據.....40 3.4胜肽合成.....44 3.4.1三胜肽分子庫之合成.....44 3.4.2四胜肽分子庫之合成.....46 參考文獻.....47 附錄 化合物之核磁共振光譜圖.....51 | |
| dc.language.iso | zh-TW | |
| dc.subject | 抑制劑 | zh_TW |
| dc.subject | 固相胜肽 | zh_TW |
| dc.subject | 合成 | zh_TW |
| dc.subject | 酪胺酸 | zh_TW |
| dc.subject | solid phase peptide synthesis | en |
| dc.subject | SH2 domain | en |
| dc.subject | Phosphotyrosine | en |
| dc.title | 合成含有仿磷酸酪胺酸的三胜肽和四胜肽作為蛋白質SH2 結構區的抑制劑 | zh_TW |
| dc.title | Synthesis of Phosphotyrosine Mimetic-Containing Tri-and Tetrapeptides as SH2 Domain Inhibitors | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林敬哲,吳世雄 | |
| dc.subject.keyword | 固相胜肽,合成,酪胺酸,抑制劑, | zh_TW |
| dc.subject.keyword | SH2 domain,Phosphotyrosine,solid phase peptide synthesis, | en |
| dc.relation.page | 108 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2010-08-11 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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