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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 譚婉玉(Woan-Yuh Tarn) | |
| dc.contributor.author | Ting-An Lin | en |
| dc.contributor.author | 林庭安 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:14:22Z | - |
| dc.date.copyright | 2010-09-09 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-11 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22249 | - |
| dc.description.abstract | TRAP150起初被發現為轉錄活化複合體TRAP/Mediator的一個次單位。此外，TRAP150亦被發現為剪接體的其中一員，說明其可能在訊息核醣核酸剪接中扮演特定的角色。我們先前的研究指出，TRAP150可與其他剪接分子共同位於細胞核內的核斑點中，並活化剪接的過程。TRAP150可與完成剪接的訊息核醣核酸結合，並與數個外顯子接合複合體的組成蛋白及參與訊息核醣核酸運送出核的受器TAP有直接的交互作用。意料外地，當TRAP150被約束在先驅訊息核醣核酸的三端非轉譯區上時，可促進細胞核內此訊息核醣核酸的降解。我們試圖對TRAP150所引發的訊息核醣核酸的降解機制有更深入的了解。我們發現到不論TRAP150被指派到訊息核醣核酸五端的非轉譯區或是內含子上都能有促進訊息核醣核酸降解的效果，並且此作用似乎不依賴轉譯，顯示這並非典型的無意義誘導訊息核醣核酸降解的機制。另外，染色質免疫沉澱的實驗結果指出TRAP150座落在基因上的各個區域。由於它可與一些轉錄複合體的組成蛋白或參與訊息核醣核酸修飾的分子進行結合，因此，TRAP150可能會協調細胞核內核醣核酸處理過程的不同步驟，並使被異常加工的核醣核酸被降解。但TRAP150對於核內訊息核醣核酸修飾的確切作用仍需進一步的研究。 | zh_TW |
| dc.description.abstract | TRAP150 has been identified as a subunit of the transcription regulatory complex TRAP/Mediator, and also a component of the spliceosome. TRAP150 contains an arginine/serine-rich domain and has sequence similarity with the cell death-promoting transcriptional repressor BCLAF1. My colleagues found that TRAP150 colocalizes with splicing factors in nuclear speckles and activates splicing in vivo. TRAP 150 remains associated with the spliced mRNA after splicing, and accordingly, it interacts with the integral exon-junction complex. Unexpectedly, when tethered to the 3’end of a precursor mRNA, TRAP150 can trigger mRNA degradation in the nucleus. However, unlike nonsense-mediated decay, TRAP150-mediated mRNA decay is irrespective of the presence of upstream stop codon and occurs in the nucleus. I attempted to understand more about the mechanism underlying TRAP150-mediated mRNA degradation. I found that TRAP150 induced mRNA degradation no matter whether it was tethered to the 5’-UTR, coding region, or intron, even for the mRNA lacking long open reading frames. Chromatin-immunoprecipitation showed that TRAP150 localized throughout the gene. Because TRAP150 interacted with several transcriptional complex and RNA processing factors, it may function in coordinating different steps of nuclear mRNA processing and perhaps target aberrantly processed mRNAs for degradation. How TRAP150 exactly acts in nuclear mRNA processing needs further studies. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:14:22Z (GMT). No. of bitstreams: 1 ntu-99-R97448006-1.pdf: 1774185 bytes, checksum: 0dd18e4bd7fd1cfa3e219286f9ad9e7b (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iii Abstract iv Chapter I: characterization of TRAP150 in mRNA degradation 1 1. INTRODUCTION 2 1.1. Co-transcriptional mRNP assembly and pre-mRNA processing 3 1.2. The TREX complex in mRNP biogenesis 7 1.3. The SAGA complex in epigenetic crosstalk 9 1.4. RNA-quality control 12 1.4.1. RNA-quality control by the exosome 13 1.4.2. Qulity control of nuclear RNAs 14 1.4.3. Qulity control of cytoplasmic RNAs 15 2. MATERIALS & METHODS 18 2.1. Plasmids 18 2.2. Cell culture and transfection 19 2.3. Chromatin immunoprecipitation 19 2.4. Immunoprecipitation and RT-PCR 22 2.5. NMD assays and northern blotting 24 2.6. In vitro polyadenylation and 3’end processing assay 24 3. RESULTS 27 3.1. TRAP150 is present throughout the genes 27 3.2. TRAP150 associates with pol II and transcriptional complex 28 3.3. TRAP150 induces mRNA decay in the tethering assay 29 3.4. In vitro polyadenylation and 3’end-processing assay in the presence of tethered TRAP150 31 4. DISCUSSIONS 34 4.1. Role of TRAP150 in mRNA decay 34 4.2. TRAP150 is a dual function modular protein 41 Chapter II: studying the role of Xrn2 in P body biogenesis 43 Abstract 44 1. INTRODUCTION 45 1.1. XRNs: the 5’→3’ exoribonucleases with important functions in RNA metabolism and RNA interference 45 1.2. P bodies and the control of mRNA translation and degradation 46 1.3. Post-transcriptional regulation by microRNAs 49 2. RESULTS 52 2.1. Knockdown of XRN2 results in the disappearance of P-bodies 52 2.2. Pursuance of the possible participation of Xrn2 in miRNA biogenesis 52 3. DISCUSSIONS 54 References 58 Figures 71 | |
| dc.language.iso | en | |
| dc.subject | 核醣核酸降解 | zh_TW |
| dc.subject | mRNA degradation | en |
| dc.subject | TRAP150 | en |
| dc.title | TRAP150於mRNA降解的特性分析 | zh_TW |
| dc.title | Characterization of the role of TRAP150 in mRNA degradation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李芳仁(Fang-Jen Lee),阮麗蓉(Li-Jung Juan) | |
| dc.subject.keyword | 核醣核酸降解, | zh_TW |
| dc.subject.keyword | TRAP150,mRNA degradation, | en |
| dc.relation.page | 85 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2010-08-12 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
| 顯示於系所單位： | 分子醫學研究所 | |
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