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標題: | 常壓非熱電漿對蔗渣水解液進行解毒生產生質酒精 Detoxification of Sugarcane Bagasse Hydrolysate by Atmospheric Cold Plasma for Bioethanol Production |
作者: | Tai-Ching Kuo 郭岱青 |
指導教授: | 鄭光成 |
關鍵字: | 木質纖維素,生質酒精,甘蔗渣,常溫電漿,解毒, lignocellulose,bioethanol,sugarcane bagasse,cold plasma,detoxification, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 生質酒精具有取代石化燃料的潛能,屬於其中一種再生性能源。相較於利用糧食作物為原料,以農林業副產物作為原料所生產的木質纖維素酒精,可避免排擠糧食供應的疑慮。由於木質纖維素資材質地堅固,無法直接為微生物所發酵利用,故於發酵前需先經過預處理及水解步驟以破壞結構並釋放出可發酵糖。然而在此過程中可能生成部份不利後續酵母菌發酵的有毒抑制物,使得生質酒精產量降低。本研究以甘蔗渣作為原料,以 1.25%的硫酸水溶液進行混合,並於 121oC 的環境中進行 2 小時的預處理,待蔗渣烘乾後再以 2%的硫酸進行酸水解,此法所製備之蔗渣水解液在纖維素水解率上可達 62%。另外,此蔗渣水解液中可測得部份有毒抑制物,包含甲酸 0.94 g/L、乙酸 2.73 g/L、糠醛 0.36 g/L 及羥甲基糠醛 1.28 g/L,在常壓非熱電漿功率 200 W、25 分鐘處理時間的條件下可有效降解 31%甲酸、45%乙酸、100%糠醛及 80%羥甲基糠醛,順利降至酵母菌可耐受範圍。將電漿處理後之蔗渣水解液進行發酵,其酒精產率可自 0.25 g/L/h 提升至 0.65 g/L/h,大幅縮減生質酒精生產時程及成本。從本研究中顯示常壓非熱電漿具有降解木質纖維素水解液中有毒抑制物的能力,並可因此提高生質酒精的產量。我們期待此技術日後於再生能源領域中所能帶來的貢獻。 Bioethanol offers a sustainable solution for transition from fossil-based fuels to renewable alternatives. The one produced from agricultural and forest residues, which is called lignocellulosic bioethanol, shows less conflicts with the food supply compared tothe one made from food crops. Since the recalcitrant structure of lignocellulosic material, the process of pretreatment and hydrolysis should be done prior to fermentation. However, toxic compounds that inhibit fermentation were also produced during the process, which reduce the productivity of bioethanol. In this study, sugarcane bagasse was chosen as lignocellulosic material and was first pretreated by 1.25% H2SO4 at 121oC for 2 hours. Later a diluted acid hydrolysis was conducted by using 2% H2SO4 before fermentation. The hydrolysis rate of the sugarcane hydrolysate could reach 62%. Within the hydrolysate, inhibitory compounds such as formic acid, acetic acid, furfural and hydroxymethylfurfural (HMF) were detected at the concentration of 0.94, 2.73, 0.36, 1.28 g/L, respectively. By applying atmospheric cold plasma for detoxification, 31% of formic acid, 45% of acetic acid, 80% of HMF and 100% of furfural were degraded under 200W treatment for 25 minutes, which the toxicity degree can be tolerated by fermenting yeast. While applied plasma-treated hydrolysate in fermentation, the ethanol productivity enhanced from 0.25 g/L/h to 0.65 g/L/h, which can considerably improve the efficiency in industry. Our results demonstrate that atmospheric cold plasma could effectively degrade the inhibitors within hydrolysate, therefore enhance the productivity of bioethanol. We anticipate the great potential of the technique in the field of renewable energy. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21223 |
DOI: | 10.6342/NTU201903616 |
全文授權: | 未授權 |
顯示於系所單位: | 生物科技研究所 |
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