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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭光成(Kuan-Chen Cheng) | |
dc.contributor.author | Chih-Chan Chou | en |
dc.contributor.author | 周志展 | zh_TW |
dc.date.accessioned | 2021-06-08T03:28:44Z | - |
dc.date.copyright | 2019-08-19 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-18 | |
dc.identifier.citation | Abeer, M. M., Mohd Amin, M. C., & Martin, C. (2014). A review of bacterial cellulose-based drug delivery systems: their biochemistry, current approaches and future prospects. J Pharm Pharmacol, 66(8), 1047-1061.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21209 | - |
dc.description.abstract | 細菌纖維素( Bacterial cellulose, BC ) 是一種具有優良物理性質,例如高抗壓強度、高保水性以及高生物相容性的生物聚合物,在許多應用領域的開發潛力。本實驗採用Komactobacter intermedius BCRC 910677作為纖維素生產的菌株 ,透過反應曲面法( Response surface methodlogy, RSM ) 優化培養基提升細菌纖維素產量。利用果糖和蛋白腖作為碳、氮源而得到相較於其他碳、氮源有著相對較佳的產量的結果。本實驗並利用Box-Behnken design (BBD) 作為反應曲面法 (RSM) 的實驗設計並分別以果糖濃度、蛋白腖濃度以及 pH 值作為參數優化培養基組成。依據 RSM 的理論最佳條件而設計最佳化培養基為果糖 41 g/L蛋白腖38 g/L 及 pH 5.2 促使最終產量相較於Hestrin-Schramm (HS) 培養基提升3.8 倍,證實利用統計方法透過 RSM 的評估設計調整培養基成分是提升細菌纖維素產量的有效方式。另外透過掃描式電子顯微鏡 (Scanning electron microscope, SEM) 觀察,使用優化培養基生成的纖維平均寬度 (38 nm) 以及纖維寬度分布 (14-70 nm) 皆低於HS 培養基 (50 nm 以及21-147 nm )。 | zh_TW |
dc.description.abstract | Bacterial cellulose (BC) is a biopolymer with extraordinary material properties such as high tensile strength, high water holding capability and high bio-compatibility; thus possessing great potential for diverse applications. This study aims to enhance BC productivity of an indigenous cellulose producing bacteria Komactobacter intermedius BCRC 910677 via response surface methodology (RSM) optimized medium. Fructose and peptone were used as carbon and nitrogen sources respectively for this medium as they both showed good productivity compared to other carbon and nitrogen sources in the initial test. In RSM study, Box-Behnken design (BBD) is applied for experimental design as the three factors, fructose concentrations, peptone concentrations and pH values were tested in order to obtain optimal medium composition for BC production. The resulting optimized medium contains fructose 41 g/L, peptone 38 g/L at pH 5.2 and increased 3.8 fold production compared to Hestrin-Schramm (HS) medium. In this study it can be seen that RSM optimized composition is a promising apprpaoch to increase BC productivity. Inspection under scanning electron microscope (SEM) reveals that fiber diameter range from 14-70 nm, with an average diameter of 38 nm, while the range for fiber diameter of the HS medium was 21-147 nm with an average of 50 nm. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T03:28:44Z (GMT). No. of bitstreams: 1 ntu-108-R05642015-1.pdf: 3979986 bytes, checksum: 811414c17144d9d95c4322fa02c60b9c (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 摘要 i
Abstract ii Table of contents iii Figures vi Tables viii 1. Introduction 1 2. Literature review 3 2.1 General outline 3 2.2 Bacterial cellulose 3 2. 3 Bacterial cellulose biosynthesis 7 2.3.1. Biosynthesis of bacterial cellulose 7 2.3.2 Komagataeibacter 12 2.3.3 K. intermedius BCRC 910677 12 2.4 Production of bacterial cellulose 13 2.4.1 Static culture 13 2.4.2 Submerged culture 13 2.4.3 Culture media 15 2.4.4. Additives 18 2.4.5. Response surface methodology 19 2.5 Application of bacterial cellulose 20 2.5.1 Food 20 2.5.2 Pharmaceutical applications 21 2.5.3 Paper additives 21 2.5.4 Acoustic diagram 22 2.5.6 Energy storage 22 2.5.6 Other applications 23 3. Frame work 28 3.1 Graphical abstract 28 3.2 Frame work 29 4. Material and methods 30 4.1 Materials 30 4.1.1 Chemical reagents 30 4.1.2 Equipments 30 4.2 Methods 31 4.2.1 Microorganism 31 4.2.2 Medium preparation 31 4.2.3 BC cultivation and harvest 32 4.2.4 Response surface methodology 32 4.2.5 Scanning Electron Microscope 32 4.2.6 Statistical analysis 33 5. Results and discussion 33 5.1 The effect of different culturing times 33 5.2 Optimal carbon sources 34 5.3 Optimal nitrogen sources 38 5.4 Response surface methodology 39 5.4.1 Optimal pH 39 5.4.2 Carbon source concentration 42 5.4.3 Nitrogen source concentration 43 5.4.4 Response surface methodology with Box-Behnken design 45 5.5 Scanning Electron Microscope 55 6. Conclusion 60 7. Future work 62 8. References 63 Index ix | |
dc.language.iso | en | |
dc.title | 以反應曲面法提升 Komactobacter intermedius 之細菌纖維素產量 | zh_TW |
dc.title | Enhanced Bacterial Cellulose Production by Komactobacter intermedius Using Response Surface Methodology | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉?睿,劉啟德,丁俞文,邱致穎,陳彥卉 | |
dc.subject.keyword | 細菌纖維素,Komactobacter intermedius,反應曲面法,優化,材料性質分析, | zh_TW |
dc.subject.keyword | Bacterial cellulose,Komactobacter intermedius,response surface methodology (RSM),optimization,material property analysis, | en |
dc.relation.page | 117 | |
dc.identifier.doi | 10.6342/NTU201903699 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2019-08-18 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
顯示於系所單位: | 生物科技研究所 |
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