請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20989完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭秋萍(Chiu-Ping Cheng) | |
| dc.contributor.author | Yi-Min Li | en |
| dc.contributor.author | 李憶敏 | zh_TW |
| dc.date.accessioned | 2021-06-08T03:14:08Z | - |
| dc.date.copyright | 2017-02-20 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-02-11 | |
| dc.identifier.citation | 趙鐸駿(2009).一個番茄鋅指蛋白基因之鑑任與功能分析。國立台灣大學植物科學研究所碩士論文。
蔡詠竹(2012).番茄microtubule-associated RING E3 ligase 1之功能分析。國立台灣大學植物科學研究所碩士論文。 何宜學(2014).新穎微管E3 ligase RED基因群之功能分析。國立台灣大學植物科學研究所碩士論文。 Alexander, M.P. (1969). Differential staining of aborted and nonaborted pollen. Stain Tech. 44, 117-122. Ardley, H.C., and Robinson, P.A. (2005). E3 ubiquitin ligases. Essays Biochem. 41, 15-30. Ardley, H.C., Tan, N.G., Rose, S.A., Markham, A.F., and Robinson, P.A. (2001). Features of the parkin/ariadne-like ubiquitin ligase, HHARI, that regulate its interaction with the ubiquitin-conjugating enzyme, Ubch7. J. Biol. Chem. 276, 19640-19647. Beck, M., Komis, G., Muller, J., Menzel, D., and Samaj, J. (2010). Arabidopsis homologs of nucleus- and phragmoplast-localized kinase 2 and 3 and mitogen-activated protein kinase 4 are essential for microtubule organization. Plant cell 22, 755-771. Bisgrove, S.R., Lee, Y.R., Liu, B., Peters, N.T., and Kropf, D.L. (2008). The microtubule plus-end binding protein EB1 functions in root responses to touch and gravity signals in Arabidopsis. Plant Cell 20, 396-410. Boutte, Y., Vernhettes, S., and Satiat-Jeunemaitre, B. (2007). Involvement of the cytoskeleton in the secretory pathway and plasma membrane organisation of higher plant cells. Cell Biol. Int. 31, 649-654. Buchwald, G., van der Stoop, P., Weichenrieder, O., Perrakis, A., van Lohuizen, M., and Sixma, T.K. (2006). Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b. EMBO J. 25, 2465-2474. Cao, L., Wang, L., Zheng, M., Cao, H., Ding, L., Zhang, X., and Fu, Y. (2013). Arabidopsis AUGMIN subunit8 is a microtubule plus-end binding protein that promotes microtubule reorientation in hypocotyls. Plant cell 25, 2187-2201. Cai, S., and Lashbrook, C.C. (2008). Stamen abscission zone transcriptome profiling reveals new candidates for abscission control: enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis ZINC FINGER PROTEIN2. Plant Physiol. 146, 1305-1321. Chan, J., Calder, G.M., Doonan, J.H., and Lloyd, C.W. (2003). EB1 reveals mobile microtubule nucleation sites in Arabidopsis. Nat. Cell Biol. 5, 967-971. Collings, D.A., Lill, A.W., Himmelspach, R., and Wasteneys, G.O. (2006). Hypersensitivity to cytoskeletal antagonists demonstrates microtubule-microfilament cross-talk in the control of root elongation in Arabidopsis thaliana. New Phytol. 170, 275-290. de Bie, P., and Ciechanover, A. (2011). Ubiquitination of E3 ligases: self-regulation of the ubiquitin system via proteolytic and non-proteolytic mechanisms. Cell Death Differ. 18, 1393-1402. de Forges, H., Bouissou, A., and Perez, F. (2012). Interplay between microtubule dynamics and intracellular organization. Int. J. Biochem. Cell Biol. 44, 266-274. de Graaf, P., Little, N.A., Ramos, Y.F., Meulmeester, E., Letteboer, S.J., and Jochemsen, A.G. (2003). Hdmx protein stability is regulated by the ubiquitin ligase activity of Mdm2. J. Biol. Chem. 278, 38315-38324. Deshaies, R.J., and Joazeiro, C.A. (2009). RING domain E3 ubiquitin ligases. Annu. Rev. Biochem. 78, 399-434. Ding, S., Zhang, B., and Qin, F. (2015). Arabidopsis RZFP34/CHYR1, a ubiquitin E3 ligase, regulates stomatal movement and drought tolerance via SnRK2.6-mediated phosphorylation. Plant cell 27, 3228-3244. Fan, L.M., Wang, Y.F., Wang, H., and Wu, W.H. (2001). In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts. J. Exp. Bot. 52, 1603-1614. Feltham, R., Bettjeman, B., Budhidarmo, R., Mace, P.D., Shirley, S., Condon, S.M., Chunduru, S.K., McKinlay, M.A., Vaux, D.L., Silke, J., and Day, C.L. (2011). Smac mimetics activate the E3 ligase activity of cIAP1 protein by promoting RING domain dimerization. J. Biol. Chem. 286, 17015-17028. Feng, J. (2006). Microtubule: a common target for parkin and Parkinson's disease toxins. Neuroscientist 12, 469-476. Foissner, I., Menzel, D., and Wasteneys, G.O. (2009). Microtubule-dependent motility and orientation of the cortical endoplasmic reticulum in elongating characean internodal cells. Cell Motil. Cytoskeleton 66, 142-155. Fu, Y. (2015). The cytoskeleton in the pollen tube. Curr. Opin. Plant Biol. 28, 111-119. Hashizume, R., Fukuda, M., Maeda, I., Nishikawa, H., Oyake, D., Yabuki, Y., Ogata, H., and Ohta, T. (2001). The RING heterodimer BRCA1-BARD1 is a ubiquitin ligase inactivated by a breast cancer-derived mutation. J. Biol. Chem. 276, 14537-14540. Hershko, A., and Ciechanover, A. (1998). The ubiquitin system. Annu. Rev. Biochem. 67, 425-479. Hotta, T., Kong, Z., Ho, C.M., Zeng, C.J., Horio, T., Fong, S., Vuong, T., Lee, Y.R., and Liu, B. (2012). Characterization of the Arabidopsis augmin complex uncovers its critical function in the assembly of the acentrosomal spindle and phragmoplast microtubule arrays. Plant Cell 24, 1494-1509. Iyengar, P.V., Hirota, T., Hirose, S., and Nakamura, N. (2011). Membrane-associated RING-CH 10 (MARCH10 protein) is a microtubule-associated E3 ubiquitin ligase of the spermatid flagella. J. Biol. Chem. 286, 39082-39090. Jia, H., Li, J., Zhu, J., Fan, T., Qian, D., Zhou, Y., Wang, J., Ren, H., Xiang, Y., and An, L. (2013). Arabidopsis CROLIN1, a novel plant actin-binding protein, functions in cross-linking and stabilizing actin filaments. J. Biol. Chem. 288, 32277-32288. Johnson, A.E., Collier, S.E., Ohi, M.D., and Gould, K.L. (2012). Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic checkpoint function. J. Biol. Chem. 287, 25741-25748. Kawamura, E., Himmelspach, R., Rashbrooke, M.C., Whittington, A.T., Gale, K.R., Collings, D.A., and Wasteneys, G.O. (2006). MICROTUBULE ORGANIZATION 1 regulates structure and function of microtubule arrays during mitosis and cytokinesis in the Arabidopsis root. Plant Physiol. 140, 102-114. Kawashima, T., and Berger, F. (2014). Epigenetic reprogramming in plant sexual reproduction. Nat. Rev. Genet. 15, 613-624. Keerthisinghe, S., Nadeau, J.A., Lucas, J.R., Nakagawa, T., and Sack, F.D. (2015). The Arabidopsis leucine-rich repeat receptor-like kinase MUSTACHES enforces stomatal bilateral symmetry in Arabidopsis. Plant J. 81, 684-694. Khanna, R., Li, J., Tseng, T.S., Schroeder, J.I., Ehrhardt, D.W., and Briggs, W.R. (2014). COP1 jointly modulates cytoskeletal processes and electrophysiological responses required for stomatal closure. Mol. Plant 7, 1441-1454. Komaki, S., Abe, T., Coutuer, S., Inze, D., Russinova, E., and Hashimoto, T. (2010). Nuclear-localized subtype of end-binding 1 protein regulates spindle organization in Arabidopsis. J. Cell Sci. 123, 451-459. Komander, D., and Rape, M. (2012). The ubiquitin code. Annu. Rev. Biochem. 81, 203-229. Kopczak, S.D., Haas, N.A., Hussey, P.J., Silflow, C.D., and Snustad, D.P. (1992). The small genome of Arabidopsis contains at least six expressed alpha-tubulin genes. Plant cell 4, 539-547. Krtkova, J., Benakova, M., and Schwarzerova, K. (2016). Multifunctional microtubule-associated proteins in plants. Front. Plant Sci. 7, 474. Lansbergen, G., and Akhmanova, A. (2006). Microtubule plus end: a hub of cellular activities. Traffic 7, 499-507. Lee, K., and Seo, P.J. (2014). The Arabidopsis E3 ubiquitin ligase HOS1 contributes to auxin biosynthesis in the control of hypocotyl elongation. Plant Growth Regul. 76, 157-165. Ligon, L.A., Shelly, S.S., Tokito, M.K., and Holzbaur, E.L. (2006). Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes. FEBS letters 580, 1327-1332. Li, W., Ahn, I.P., Ning, Y., Park, C.H., Zeng, L., Whitehill, J.G., Lu, H., Zhao, Q., Ding, B., Xie, Q., Zhou, J.M., Dai, L., and Wang, G.L. (2012). The U-Box/ARM E3 ligase PUB13 regulates cell death, defense, and flowering time in Arabidopsis. Plant Physiol. 159, 239-250. Lim, S.D., Jung, C.G., Park, Y.C., Lee, S.C., Lee, C., Lim, C.W., Kim, D.S., and Jang, C.S. (2015). Molecular dissection of a rice microtubule-associated RING finger protein and its potential role in salt tolerance in Arabidopsis. Plant Mol. Biol. 89, 365-384. Lindeboom, J.J., Nakamura, M., Hibbel, A., Shundyak, K., Gutierrez, R., Ketelaar, T., Emons, A.M., Mulder, B.M., Kirik, V., and Ehrhardt, D.W. (2013). A mechanism for reorientation of cortical microtubule arrays driven by microtubule severing. Sci. 342, 1245533. Ling, Q., and Jarvis, P. (2016). Plant signaling: ubiquitin pulls the trigger on chloroplast degradation. Curr. Biol. 26, R38-40. Linke, K., Mace, P.D., Smith, C.A., Vaux, D.L., Silke, J., and Day, C.L. (2008). Structure of the MDM2/MDMX RING domain heterodimer reveals dimerization is required for their ubiquitylation in trans. Cell Death Differ. 15, 841-848. Liu, H., and Stone, S.L. (2010). Abscisic acid increases Arabidopsis ABI5 transcription factor levels by promoting KEG E3 ligase self-ubiquitination and proteasomal degradation. The Plant cell 22, 2630-2641. Liu, J., Zhang, C., Wei, C., Liu, X., Wang, M., Yu, F., Xie, Q., and Tu, J. (2016). The RING finger ubiquitin E3 ligase OSHTAS enhances heat tolerance by promoting H2O2-induced stomatal closure in rice. Plant physiol. 170, 429-443. Liu, J., Zhang, Y., Qin, G., Tsuge, T., Sakaguchi, N., Luo, G., Sun, K., Shi, D., Aki, S., Zheng, N., Aoyama, T., Oka, A., Yang, W., Umeda, M., Xie, Q., Gu, H., and Qu, L.J. (2008). Targeted degradation of the cyclin-dependent kinase inhibitor ICK4/KRP6 by RING-type E3 ligases is essential for mitotic cell cycle progression during Arabidopsis gametogenesis. Plant Cell 20, 1538-1554. Liu, L., Zhang, Y., Tang, S., Zhao, Q., Zhang, Z., Zhang, H., Dong, L., Guo, H., and Xie, Q. (2010). An efficient system to detect protein ubiquitination by agroinfiltration in Nicotiana benthamiana. Plant J. 61, 893-903. Liu, M., Shi, S., Zhang, S., Xu, P., Lai, J., Liu, Y., Yuan, D., Wang, Y., Du, J., and Yang, C. (2014). SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis. BMC Plant Biol. 14, 153. Luo, G., Gu, H., Liu, J., and Qu, L.J. (2012). Four closely-related RING-type E3 ligases, APD1-4, are involved in pollen mitosis II regulation in Arabidopsis. J. Integr. Plant Biol.. 54, 814-827. McCormick, S. (2004). Control of male gametophyte development. Plant Cell 16 Suppl, S142-153. Metzger, M.B., Hristova, V.A., and Weissman, A.M. (2012). HECT and RING finger families of E3 ubiquitin ligases at a glance. J. Cell Sci. 125, 531-537. Metzger, M.B., Pruneda, J.N., Klevit, R.E., and Weissman, A.M. (2014). RING-type E3 ligases: master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination. Biochim. Biophys. Acta 1843, 47-60. Miao, M., Niu, X., Kud, J., Du, X., Avila, J., Devarenne, T.P., Kuhl, J.C., Liu, Y., and Xiao, F. (2016). The ubiquitin ligase SEVEN IN ABSENTIA (SINA) ubiquitinates a defense-related NAC transcription factor and is involved in defense signaling. New phytol. 211, 138-148. Oda, Y. (2015). Cortical microtubule rearrangements and cell wall patterning. Front. Plant Sci. 6, 236. Oh, S.A., Jeon, J., Park, H.J., Grini, P.E., Twell, D., and Park, S.K. (2016). Analysis of gemini pollen 3 mutant suggests a broad function of AUGMIN in microtubule organization during sexual reproduction in Arabidopsis. Plant J. 87, 188-201. Oh, S.A., Park, K.S., Twell, D., and Park, S.K. (2010). The SIDECAR POLLEN gene encodes a microspore-specific LOB/AS2 domain protein required for the correct timing and orientation of asymmetric cell division. Plant J. 64, 839-850. Oh, S.A., Twell, D., and Park, S.K. (2011). SIDECAR POLLEN suggests a plant-specific regulatory network underlying asymmetric microspore division in Arabidopsis. Plant Signal. Behav. 6, 416-419. Ohi, M.D., Vander Kooi, C.W., Rosenberg, J.A., Chazin, W.J., and Gould, K.L. (2003). Structural insights into the U-box, a domain associated with multi-ubiquitination. Nat. Struct. Biol. 10, 250-255. Pan, R., Satkovich, J., and Hu, J. (2016). E3 ubiquitin ligase SP1 regulates peroxisome biogenesis in Arabidopsis. Proc. Natl. Acad. Sci. USA 113, E7307-E7316. Park, G.T., Frost, J.M., Park, J.S., Kim, T.H., Lee, J.S., Oh, S.A., Twell, D., Brooks, J.S., Fischer, R.L., and Choi, Y. (2014). Nucleoporin MOS7/Nup88 is required for mitosis in gametogenesis and seed development in Arabidopsis. Proc. Natl. Acad. Sci. USA 111, 18393-18398. Plechanovova, A., Jaffray, E.G., McMahon, S.A., Johnson, K.A., Navratilova, I., Naismith, J.H., and Hay, R.T. (2011). Mechanism of ubiquitylation by dimeric RING ligase RNF4. Nat. Struct. Mol. Biol. 18, 1052-1059. Poruchynsky, M.S., Sackett, D.L., Robey, R.W., Ward, Y., Annunziata, C., and Fojo, T. (2008). Proteasome inhibitors increase tubulin polymerization and stabilization in tissue culture cells: a possible mechanism contributing to peripheral neuropathy and cellular toxicity following proteasome inhibition. Cell Cycle 7, 940-949. Qin, T., Liu, X., Li, J., Sun, J., Song, L., and Mao, T. (2014). Arabidopsis microtubule-destabilizing protein 25 functions in pollen tube growth by severing actin filaments. Plant Ccell 26, 325-339. Qu, X., Zhang, H., Xie, Y., Wang, J., Chen, N., and Huang, S. (2013). Arabidopsis villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars. Plant Cell 25, 1803-1817. Qu, X., Jiang, Y., Chang, M., Liu, X., Zhang, R., and Huang, S. (2014). Organization and regulation of the actin cytoskeleton in the pollen tube. Front. Plant Sci. 5, 786. Rasmussen, C.G., Wright, A.J., and Muller, S. (2013). The role of the cytoskeleton and associated proteins in determination of the plant cell division plane. Plant J. 75, 258-269. Sadanandom, A., Bailey, M., Ewan, R., Lee, J., and Nelis, S. (2012). The ubiquitin-proteasome system: central modifier of plant signalling. New Phytol. 196, 13-28. Seo, D.H., Ahn, M.Y., Park, K.Y., Kim, E.Y., and Kim, W.T. (2016). The N-terminal UND motif of the Arabidopsis U-box E3 ligase PUB18 is critical for the negative regulation of ABA-mediated stomatal movement and determines its ubiquitination specificity for exocyst subunit Exo70B1. Plant Cell. doi:10.1105/tpc.16.00347. Shabek, N., and Zheng, N. (2014). Plant ubiquitin ligases as signaling hubs. Nat. Struct. Mol. Biol. 21, 293-296. Sheng, X., Hu, Z., Lu, H., Wang, X., Baluska, F., Samaj, J., and Lin, J. (2006). Roles of the ubiquitin/proteasome pathway in pollen tube growth with emphasis on MG132-induced alterations in ultrastructure, cytoskeleton, and cell wall components. Plant Physiol. 141, 1578-1590. Smertenko, A., and Franklin-Tong, V.E. (2011). Organisation and regulation of the cytoskeleton in plant programmed cell death. Cell Death Differ. 18, 1263-1270. Snustad, D.P., Haas, N.A., Kopczak, S.D., and Silflow, C.D. (1992). The small genome of Arabidopsis contains at least nine expressed beta-tubulin genes. Plant cell 4, 549-556. Srivastava, D., and Chakrabarti, O. (2014). Mahogunin-mediated alpha-tubulin ubiquitination via noncanonical K6 linkage regulates microtubule stability and mitotic spindle orientation. Cell death disease 5, e1064. Staiger, C.J., Poulter, N.S., Henty, J.L., Franklin-Tong, V.E., and Blanchoin, L. (2010). Regulation of actin dynamics by actin-binding proteins in pollen. J. Exp. Bot. 61, 1969-1986. Stommel, J.M., and Wahl, G.M. (2004). Accelerated MDM2 auto-degradation induced by DNA-damage kinases is required for p53 activation. EMBO J. 23, 1547-1556. Stone, S.L. (2014). The role of ubiquitin and the 26S proteasome in plant abiotic stress signaling. Front. Plant Sci. 5, 135. Stone, S.L., Hauksdottir, H., Troy, A., Herschleb, J., Kraft, E., and Callis, J. (2005). Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis. Plant Physiol. 137, 13-30. Tian, M., Lou, L., Liu, L., Yu, F., Zhao, Q., Zhang, H., Wu, Y., Tang, S., Xia, R., Zhu, B., Serino, G., and Xie, Q. (2015). The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt-stress response in Arabidopsis thaliana. Plant J. 82, 81-92. Trockenbacher, A., Suckow, V., Foerster, J., Winter, J., Krauss, S., Ropers, H.H., Schneider, R., and Schweiger, S. (2001). MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation. Nat. Genet. 29, 287-294. Trujillo, M., and Shirasu, K. (2010). Ubiquitination in plant immunity. Curr. Opin. Plant Biol. 13, 402-408. Twell, D. (2011). Male gametogenesis and germline specification in flowering plants. Sex Plant Reprod 24, 149-160. Valencia, J.P., Goodman, K., and Otegui, M.S. (2016). Endocytosis and endosomal trafficking in plants. Ann. Rev. Plant Biol. 67, 309-335. Vander Kooi, C.W., Ohi, M.D., Rosenberg, J.A., Oldham, M.L., Newcomer, M.E., Gould, K.L., and Chazin, W.J. (2006). The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases. Biochem. 45, 121-130. Wang, H., Lu, Y., Jiang, T., Berg, H., Li, C., and Xia, Y. (2013). The Arabidopsis U-box/ARM repeat E3 ligase AtPUB4 influences growth and degeneration of tapetal cells, and its mutation leads to conditional male sterility. Plant J. 74, 511-523. Wang, S., Cao, L., and Wang, H. (2016). Arabidopsis ubiquitin-conjugating enzyme UBC22 is required for female gametophyte development and likely involved in Lys11-linked ubiquitination. J. Exp. Bot. 67, 3277-3288. Wang, S., Kurepa, J., Hashimoto, T., and Smalle, J.A. (2011). Salt stress-induced disassembly of Arabidopsis cortical microtubule arrays involves 26S proteasome-dependent degradation of SPIRAL1. Plant cell 23, 3412-3427. Wang, Y., Zhang, W.Z., Song, L.F., Zou, J.J., Su, Z., and Wu, W.H. (2008). Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol. 148, 1201-1211. Wasteneys, G.O., and Yang, Y.B. (2004). New views on the plant cytoskeleton. Plant Physiol. 136, 3884-3891. Watkins, G.R., Wang, N., Mazalouskas, M.D., Gomez, R.J., Guthrie, C.R., Kraemer, B.C., Schweiger, S., Spiller, B.W., and Wadzinski, B.E. (2012). Monoubiquitination promotes calpain cleavage of the protein phosphatase 2A (PP2A) regulatory subunit alpha4, altering PP2A stability and microtubule-associated protein phosphorylation. J. Biol. Chem. 287, 24207-24215. Yadegari, R., and Drews, G.N. (2004). Female gametophyte development. Plant Cell 16 Suppl, S133-141. Yang, Y., Fu, D., Zhu, C., He, Y., Zhang, H., Liu, T., Li, X., and Wu, C. (2015). The RING-finger ubiquitin ligase HAF1 mediates HEADING DATE 1 degradation during photoperiodic flowering in rice. Plant cell 27, 2455-2468. Ye, J., Zhang, W., and Guo, Y. (2013). Arabidopsis SOS3 plays an important role in salt tolerance by mediating calcium-dependent microfilament reorganization. Plant Cell Rep. 32, 139-148. Yin, Q., Lin, S.C., Lamothe, B., Lu, M., Lo, Y.C., Hura, G., Zheng, L., Rich, R.L., Campos, A.D., Myszka, D.G., Lenardo, M.J., Darnay, B.G., and Wu, H. (2009). E2 interaction and dimerization in the crystal structure of TRAF6. Nat. Struct. Mol. Biol. 16, 658-666. Zhang, H., Cui, F., Wu, Y., Lou, L., Liu, L., Tian, M., Ning, Y., Shu, K., Tang, S., and Xie, Q. (2015). The RING finger ubiquitin E3 ligase SDIR1 targets SDIR1-INTERACTING PROTEIN1 for degradation to modulate the salt stress response and ABA signaling in Arabidopsis. Plant Cell 27, 214-227. Zhao, Q., Tian, M., Li, Q., Cui, F., Liu, L., Yin, B., and Xie, Q. (2013). A plant-specific in vitro ubiquitination analysis system. Plant J. 74, 524-533. Zheng, Y., Xie, Y., Jiang, Y., Qu, X., and Huang, S. (2013). Arabidopsis actin-depolymerizing factor7 severs actin filaments and regulates actin cable turnover to promote normal pollen tube growth. Plant Cell 25, 3405-3423. Zhou, J.J., Liang, Y., Niu, Q.K., Chen, L.Q., Zhang, X.Q., and Ye, D. (2013). The Arabidopsis general transcription factor TFIIB1 (AtTFIIB1) is required for pollen tube growth and endosperm development. J. Exp. Bot. 64, 2205-2218. Zhu, L., Zhang, Y., Kang, E., Xu, Q., Wang, M., Rui, Y., Liu, B., Yuan, M., and Fu, Y. (2013). MAP18 regulates the direction of pollen tube growth in Arabidopsis by modulating F-actin organization. Plant Cell 25, 851-867. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20989 | - |
| dc.description.abstract | 泛素化系統是生物體內相當重要之後轉譯修飾，在植物的許多功能皆是相當重要的調控機制，其中與目標蛋白結合之E3泛素接合酶 (E3 ligase) 更扮演極重要之角色，然而目前絕大多數之E3 ligase尚未被研究。本研究室先前發現在番茄與阿拉伯芥中有一群坐落於微管且功能未知之RING-H2 type E3 ligase，將其命名為RING-type E3 ligase with an uncharacterized DAR1 domain (REDs)；番茄SlRED1參與植物抵抗病原菌，且在花粉管萌發扮具正向調控功能；阿拉伯芥有三個REDs基因，其中AtRED1與AtRED2主要在花粉與精細胞表現。本研究旨在探討阿拉伯芥AtRED1/2之蛋白質特性、在生殖功能所扮演的角色及與微管相關蛋白之交互作用，並尋找其作用蛋白質，以利探討其功能與調控機制。實驗結果顯示，AtRED1/2皆具有E3 ligase活性並與E2具蛋白質交互作用，且RING domain之保守性胺基酸皆在其中扮演關鍵角色；AtRED1/2可形成homodimer與heterodimer且可能具有自我或彼此泛素化之能力。AtRED1與AtRED2之單基因突變株無生殖缺陷，但雙基因減量表現之植株其花粉粒活性與花粉管萌發率下降、果莢變短、種子發育不良、未受精胚珠比例提高，故有功能冗餘 (functional redundancy) 特性。本研究發現AtRED1/2可能與tubulins無直接交互作用，但與+TIPs AtEB1a/b具交互作用且AtEB1a/b可能為其泛素化對象，但目前尚未了解與其交互作用相關之生理功能。此外，以酵母菌雙雜交系統篩選AtRED1/2交互作用對象，但測試數個與生殖相關蛋白質後卻未能找到確切交互蛋白質。以上結果指出AtRED1/2為植物生殖功能之關鍵正向調控者，且可能藉AtEB1a/b間接座落於微管並泛素化AtEB1a/b。未來須進一步搜尋AtRED1/2之交互作用蛋白以釐清其作用機制。 | zh_TW |
| dc.description.abstract | Protein ubiquitination is an important post-translation modification and involved in various plant functions. The E3 ligases play critical roles in specific recognition of the targets; however, most E3 ligases are not characterized. In previous studies, we identified a group of uncharacterized plant microtubule-localized RING-H2 type E3 ligases, named RING-type E3 ligase with an uncharacterized DAR1 domain (REDs). SlRED1 was involved in plants resistance to pathogens and played a positive role in pollen germination. There are three RED homologs in Arabidopsis and microarray analyses revealed predominant expression of AtRED1 and AtRED2 in pollens and sperm cells. This study aimed to further determine properties of AtRED1/2, their roles in reproduction and their interactions with microtubule-associated proteins. The results revealed an essential role of the conserved residue in the RING domain for AtRED1/2 E3 activity and their interactions with the E2 ligase. In addition, AtRED1/2 may form homodimer and heterodimer, and may self-ubiquitinate or ubiquitinate each other. Furthermore, double-knockdown lines of AtRED1 and AtRED2, but not single gene mutants, displayed significant reduction in pollen viability, pollen tube germination, siliques length and seed production, suggesting functional redundancy of AtRED1/2. Moreover, AtRED1/2 may not interact with tubulins, but interacted with AtEB1a/b, a group of microtubule plus end tracking proteins and may ubiquitinate AtEB1a/b. However, the physiological function of the AtRED1/2-AtEB1a/b interactions remaine to be determined. In addition, a yeast-two-hybrid systemic screening for AtRED1/2-interacting proteins failed to identify candidates related to reproduction. Together these results uncover the critical positive role of AtRED1/2 in regulating reproduction and reveal the AtRED1/2-AtEB1a/b interactions. Further identification and study of AtRED1/2 interacting proteins would help to elucidate the underlying regulatory mechanisms for their functions. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T03:14:08Z (GMT). No. of bitstreams: 1 ntu-106-R03b42029-1.pdf: 9247544 bytes, checksum: 0b335958d4cd1fd88a5b9318e4e0f697 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 口試委員審定書 I
謝誌 II 中文摘要 IV Abstract V 常用縮寫與全名對照表 VI 目錄 VIII 圖目錄 XI 附錄目錄 XII 第一章 前言 1 1. 植物泛素化系統及其功能 1 1.1. 泛素化系統 1 1.2. 阿拉伯芥之E3 ligase 1 2. 細胞骨架與其功能 3 2.1. 細胞骨架 3 2.2. 微管結合蛋白(microtubule-associated proteins, MAPs) 4 2.3. 阿拉伯芥細胞骨架與MAPs參與之功能 5 3. 植物之生殖 5 3.1. 植物配子體之發育 5 3.2. 阿拉伯芥配子體發育之調控因子 6 3.3. 阿拉伯芥花粉管萌發之調控因子 7 4. 前人研究與研究動機 7 第二章 材料與方法 9 1. 植物材料簡介 9 2. 實驗用菌體與培養條件 9 3. 基因選殖常用實驗 10 3.1. 聚合酶連鎖反應 10 3.2. DNA 瓊脂糖凝膠電泳 10 3.3. DNA 純化 10 3.4. DNA 限制酶消化水解 11 3.5. DNA 片段接合 11 3.6. TOPO® 質體構築 11 3.7. LR 重組互換反應 11 3.8. 大腸桿菌勝任細胞熱休克轉型作用 12 3.9. 電穿孔轉型作用之勝任細胞置備 12 3.10. 電穿孔轉型作用 12 3.11. 質體萃取 12 4. 植物DNA萃取 13 5. 植物RNA萃取 13 6. 反轉錄聚合酶連鎖反應 14 7. 半定量RT-PCR 15 8. 即時定量PCR (Real-time PCR) 15 9. 植物蛋白質檢測 15 9.1. 植物蛋白質萃取 15 9.2. 蛋白質定量 15 9.3. 蛋白質電泳 16 9.4. 西方墨點法 16 10. 蛋白質降解試驗 (In planta degradation assay) 17 10.1. 構築載體 17 10.2. 菸草葉部基因短暫表現 17 11. 自體泛素化試驗 (In vitro self-ubiquitination assay) 17 11.1. 構築載體 17 11.2. 蛋白質表現 17 11.3. 蛋白質純化 18 11.4. In vitro self-ubiquitination assay 18 12. 酵母菌雜合系統 (Yeast tow-hybrid system，Y2H) 19 12.1. 構築載體 19 12.2. 製備酵母菌勝任細胞 19 12.3. 酵母菌雙雜合系統 19 13. 雙分子螢光互補 19 14. 植物生殖表現型檢測 20 14.1. 果莢與種子特性之檢測 20 14.2. 花粉活性、花粉管萌發與細胞核染色檢測 20 15. 根部反應表現型檢測 21 16. 生物統計分析 21 第三章 結果 22 1. AtRED1/2之E3 ligase活性測試 22 2. AtRED1/2之交互作用檢測 23 3. AtRED1/2是否為彼此受質之檢測 23 4. AtRED1/2對果莢與種子型態之影響 24 5. AtRED1/2對花粉特性之影響 24 6. AtRED1/2與微管相關蛋白之交互作用 25 7. AtEB1a/b是否為AtRED1/2受質之檢測 25 8. 探討AtEB1a/b在植物生殖之功能 26 9. 探討AtRED1/2在根部反應之功能 26 10. 篩選AtRED1/2之交互作用蛋白質 26 第四章 討論 28 1. RING domain在AtRED1/2酵素活性以及與E2結合具關鍵性 28 2. AtRED1/2有交互作用與相互調控關係 28 3. AtRED1/2與微管相關蛋白有交互作用與調控關係 30 4. AtRED1/2為生殖功能之關鍵調控者 31 5. 結語 33 第五章 參考文獻 34 | |
| dc.language.iso | zh-TW | |
| dc.subject | 植物生殖 | zh_TW |
| dc.subject | RED | zh_TW |
| dc.subject | 泛素化系統 | zh_TW |
| dc.subject | E3 ligase | zh_TW |
| dc.subject | 微管 | zh_TW |
| dc.subject | EB1 | zh_TW |
| dc.subject | RED | en |
| dc.subject | reproduction | en |
| dc.subject | EB1 | en |
| dc.subject | microtubule | en |
| dc.subject | E3 ligase | en |
| dc.subject | ubiquitination | en |
| dc.title | 阿拉伯芥E3 ligases REDs 在生殖功能之研究 | zh_TW |
| dc.title | Function of Arabidopsis E3 ligases REDs in reproduction | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 符宏勇,趙光裕,王中茹,林讚標 | |
| dc.subject.keyword | RED,泛素化系統,E3 ligase,微管,EB1,植物生殖, | zh_TW |
| dc.subject.keyword | RED,ubiquitination,E3 ligase,microtubule,EB1,reproduction, | en |
| dc.relation.page | 113 | |
| dc.identifier.doi | 10.6342/NTU201700514 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2017-02-13 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-106-1.pdf 未授權公開取用 | 9.03 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。