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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林文貞 | |
| dc.contributor.author | Tien-Ru Chen | en |
| dc.contributor.author | 陳恬如 | zh_TW |
| dc.date.accessioned | 2021-06-08T03:12:04Z | - |
| dc.date.copyright | 2017-09-12 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-03-10 | |
| dc.identifier.citation | Akmaz S, Adıgüzel ED, Yasar M, Erguven O. The effect of Ag content of the chitosan-silver nanoparticle composite material on the structure and antibacterial activity. Advances in Materials Science and Engineering. 2013;2013:1-6.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20951 | - |
| dc.description.abstract | 銀的抗菌性已被發現數千年之久，本篇選用具有生物相容性的幾丁聚醣在鹼性環境下還原硝酸銀成為奈米銀(CS-AgNPs)，所形成的CS-AgNPs與銀離子(Ag+)、奈米銀(AgNPs)比較其抗菌性與細胞毒性；除此之外生物實驗中也另外比較幾丁聚醣(CS)與奈米幾丁聚醣(CS-TPP NPs)。
物性測試的部分分別以粒徑分析儀、穿透式電子顯微鏡、紫外光/可見光光譜儀、紅外線分光光譜儀、火焰式原子吸收光譜進行分析。在生物性實驗的部分，以L929細胞進行細胞毒性試驗並利用細胞存活率計算IC50；抗菌性實驗檢測大腸桿菌與金黃色葡萄球菌的最小抑菌濃度(MIC)、最小殺菌濃度(MBC)與抑制圈大小。 CS-AgNPs系列中的AgNPs大小約為10 nm左右，而AgNPs與CS-TPP NPs的大小分別為40~50 nm與200~300 nm。生物性實驗比較細菌抗性與細胞毒性的結果發現，含銀系列樣品的抗菌效果與細胞毒性皆大於幾丁聚醣系列。在含銀樣品的結果中Ag+和AgNPs相互比較，MBC與抑制圈的結果呈現Ag+優於AgNPs，在細胞毒性上48小時的毒性比較未見統計差異；Ag+與CS-AgNPs相互比較，在MIC與MBC的結果發現，CS-AgNPs的抗菌性更勝Ag+，而48小時細胞毒性中Ag+與CS-AgNPs的IC50數值皆在0.3~0.5 µg/mL區間，但CS-AgNPs的IC50多小於Ag+。當CS與CS-TPP NPs相比較，在最高劑量下皆未見其細胞毒性，而抗菌性的部分則觀察到CS的抗菌效果優於CS-TPP NPs的現象。 | zh_TW |
| dc.description.abstract | The antibacterial property of silver has been discovered for thousand years. This study used biocompatible chitosan to reduce silver ion and form silver nanoparticles (CS-AgNPs). The antibacterial activity and cell toxicity among Ag+, AgNPs, and CS-AgNPs were compared. Moreover, chitosan (CS) and chitosan nanoparticles (CS-TPP NPs) were also evaluated, too.
The properties of samples were determined by using Zetasizer, TEM, UV-Vis spectroscope, FT-IR , and Flame Atomic Absorption Spectrometer. The cytotoxicity was preformed in L929 cell line and IC50 values were calculated. The antibacterial activity was evaluated in the Escherichia coli as well as Staphylococcus aureus, and the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and inhibition zone were measured. The size of AgNPs in CS-AgNPs was about 10 nm.The particle diameter of AgNPs and CS-TPP NPs was about 40~50 nm and 200~300 nm. The bacterial activity and cell cytotoxicity of silver products were higher than those of chitosan products. According to the MBC and inhibition zone results, the antibacterial activity of the Ag+ was higher than AgNPs. However, the was no significant difference in 48-hours cell toxicity. In addition, CS-AgNPs had higher antibacterial activity than Ag+ according to MIC and MBC results. The half maximal inhibitory concentrations (IC50) of Ag+ and CS-AgNPs were 0.3-0.5 µg/mL in 48-hours cell experiment, and most of the CS-AgNPs were less than Ag+. There was no cytotoxicity of CS and CS-TPP NPs, even at the highest concentration. The antibacterial activity of CS are higher than CS-TPP NPs. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T03:12:04Z (GMT). No. of bitstreams: 1 ntu-106-R02423018-1.pdf: 3640400 bytes, checksum: 37b8c74ac0c8cebe11e67ffa3200f752 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 致謝 I
中文摘要 II Abstract III 目錄 IV 表目錄 VIII 圖目錄 IX 第一章 緒論 1 一、銀 1 二、奈米銀(AgNPs) 1 三、AgNPs的毒性機轉 5 四、Ag+的毒性機轉 7 五、AgNPs的性質影響其抗菌性的作用性(Liu and Jiang, 2015) 8 六、Ag與AgNPs的應用 9 七、Ag與AgNPs的危害 12 （一）、估計暴露於含銀物質的量 12 （二）、對人的毒性 13 八、幾丁聚醣(chitosan) 14 九、幾丁聚醣的特性 14 十、幾丁聚醣應用 16 十一、三聚磷酸鈉(Tripolyphosphate, TPP) 18 十二、軟骨素(chondroitin sulfate) 19 第二章 實驗動機與目的 20 第三章 實驗試劑與儀器 21 一、藥品 21 二、細胞實驗材料 22 三、細菌實驗材料 23 四、儀器 23 五、耗材 25 六、藥品溶液與緩衝溶液製備 25 第四章 實驗方法 27 一、幾丁聚醣之製備 30 （一）、去乙醯幾丁聚醣(DACS)之製備 (Kurita, 2001) 30 （二）、去乙醯去聚合幾丁聚醣(DADPCS)之製備 (Mao et al., 2004) 30 （三）、去聚合幾丁聚醣(DPCS)之製備 (Mao et al., 2004) 31 二、幾丁聚醣的物性測定 31 （一）、傅立葉轉換紅外線光譜儀(FT-IR)：鑑別官能基 31 （二）、膠體滲透層析(GPC)：分子量評估 32 三、製備含奈米銀之幾丁聚醣複合體(CS-AgNPs) (Akmaz et al., 2013 ; Chen et al., 2014) 33 四、含奈米銀之幾丁聚醣複合體(CS-AgNPs)物性測試 33 （一）、粒徑與表面電位分析 33 （二）、紫外光/可見光光譜儀(UV/Visible Spectrophotometer)：是否形成奈米銀 33 （三）、傅立葉轉換紅外線光譜儀(FT-IR)：鑑別官能基 34 （四）、穿透式電子顯微鏡 (TEM)：觀察樣品外觀 34 （五）、火焰式原子吸收光譜儀：分析樣品中的銀含量 34 （六）、安定性試驗 35 五、奈米銀的製備(Rashid et al., 2013) 35 六、奈米幾丁聚醣的製備 (Gan et al., 2005) 36 七、細胞毒性試驗 38 八、細菌實驗 40 （一）、最小抑菌濃度(Minimum Inhibitory Concentration, MIC) (Wiegand et al., 2008) 40 （二）、最小殺菌濃度(Minimum Bactericidal Concentration, MBC) (Mofazzal Jahromi et al., 2013) 42 （三）、紙錠擴散法(Disc-diffusion method)：觀察抑制圈(Inhibition zone) (Wang et al., 2015) 42 九、統計分析 42 第五章 實驗結果 43 一、幾丁聚醣之製備 43 （一）、幾丁聚醣之官能基探討 43 （二）、幾丁聚醣之分子量探討 46 二、含奈米銀之奈米複合體 48 （一）、粒徑與電位分析 48 （二）、紫外光/可見光光譜儀(UV/Visible Spectrophotometer)分析 50 （三）、傅立葉轉換紅外線光譜儀(FT-IR) 52 （四）、穿透式電子顯微鏡 (TEM) 54 （五）、火焰式原子吸收光譜儀 56 （六）、安定性試驗 58 三、奈米銀 62 四、奈米幾丁聚醣 65 （一）、粒徑與表面電位分析 65 五、細胞毒性試驗 70 六、抗菌試驗 97 （一）、最小抑菌濃度(MIC) 97 （二）、最小殺菌濃度(MBC) 100 （三）、紙錠擴散法(Disk-diffusion method) 103 第六章 結論 107 第七章 參考文獻 110 | |
| dc.language.iso | zh-TW | |
| dc.subject | 幾丁聚醣 | zh_TW |
| dc.subject | 銀 | zh_TW |
| dc.subject | 奈米粒子 | zh_TW |
| dc.subject | 抗菌 | zh_TW |
| dc.subject | 毒性 | zh_TW |
| dc.subject | toxicity | en |
| dc.subject | antibacterial | en |
| dc.subject | nanoparticle | en |
| dc.subject | silver | en |
| dc.subject | chitosan | en |
| dc.title | 奈米銀與幾丁聚醣的抗菌性研究 | zh_TW |
| dc.title | Antibacterial study of silver and chitosan nanoparticles | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃義侑,王健珍 | |
| dc.subject.keyword | 幾丁聚醣,銀,奈米粒子,抗菌,毒性, | zh_TW |
| dc.subject.keyword | chitosan,silver,nanoparticle,antibacterial,toxicity, | en |
| dc.relation.page | 116 | |
| dc.identifier.doi | 10.6342/NTU201700679 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2017-03-10 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 藥學研究所 | zh_TW |
| 顯示於系所單位： | 藥學系 | |
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