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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 鄭景暉 | |
dc.contributor.author | Chia-Mei Tang | en |
dc.contributor.author | 唐佳鋂 | zh_TW |
dc.date.accessioned | 2021-06-08T02:57:01Z | - |
dc.date.copyright | 2017-09-08 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-01 | |
dc.identifier.citation | Abraham, R. T. (2001). Cell cycle checkpoint signaling through the ATM and ATR kinases. Genes development, 15(17), 2177-2196. Ågren, M. (1993). Zinc oxide increases degradation of collagen in necrotic wound tissue. British Journal of Dermatology, 129(2), 221-222. Bartek, J., Lukas, J. (2003). Chk1 and Chk2 kinases in checkpoint control and cancer. Cancer Cell, 3(5), 421-429. Boonstra, J. (2003). Progression through the G1-phase of the on-going cell cycle. J Cell Biochem, 90(2), 244-252. Boonstra, J., Post, J. A. (2004). Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells. Gene, 337, 1-13. Camargo, C. H., Camargo, S. E., Valera, M. C., Hiller, K. A., Schmalz, G., Schweikl, H. (2009). The induction of cytotoxicity, oxidative stress, and genotoxicity by root canal sealers in mammalian cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 108(6), 952-960. Cerutti, P. A. (1985). Prooxidant states and tumor promotion. Science, 227, 375-382. Chang, M. C., Lin, L. D., Chen, Y. J., Tsai, Y. L., Cheng, Y. A., Kuo, C. S., . . . Jeng, J. H. (2010). Comparative cytotoxicity of five root canal sealers on cultured human periodontal ligament fibroblasts. Int Endod J, 43(3), 251-257. Chi, P. L., Lin, C. C., Chen, Y. W., Hsiao, L. D., Yang, C. M. (2015). CO Induces Nrf2-Dependent Heme Oxygenase-1 Transcription by Cooperating with Sp1 and c-Jun in Rat Brain Astrocytes. Mol Neurobiol, 52(1), 277-292. Chithrani, B. D., Ghazani, A. A., Chan, W. C. (2006). Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano letters, 6(4), 662-668. Choi, S. J., Choy, J. H. (2014). Biokinetics of zinc oxide nanoparticles: toxicokinetics, biological fates, and protein interaction. Int J Nanomedicine, 9 Suppl 2, 261-269. Condello, M., De Berardis, B., Ammendolia, M. G., Barone, F., Condello, G., Degan, P., Meschini, S. (2016). ZnO nanoparticle tracking from uptake to genotoxic damage in human colon carcinoma cells. Toxicol In Vitro, 35, 169-179. Deng, X., Luan, Q., Chen, W., Wang, Y., Wu, M., Zhang, H., Jiao, Z. (2009). Nanosized zinc oxide particles induce neural stem cell apoptosis. Nanotechnology, 20(11), 115101. Economides, N., Kotsaki-Kovatsi, V.-P., Poulopoulos, A., Kolokuris, I., Rozos, G., Shore, R. (1995). Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues. Journal of Endodontics, 21(3), 122-127. Fubini, B., Hubbard, A. (2003). Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis. Free Radical Biology and Medicine, 34(12), 1507-1516. doi:10.1016/s0891-5849(03)00149-7 Fukui, H., Horie, M., Endoh, S., Kato, H., Fujita, K., Nishio, K., . . . Iwahashi, H. (2012). Association of zinc ion release and oxidative stress induced by intratracheal instillation of ZnO nanoparticles to rat lung. Chem Biol Interact, 198(1-3), 29-37. Gao, F., Ma, N., Zhou, H., Wang, Q., Zhang, H., Wang, P., . . . Li, L. (2016). Zinc oxide nanoparticles-induced epigenetic change and G2/M arrest are associated with apoptosis in human epidermal keratinocytes. Int J Nanomedicine, 11, 3859-3874. Geurtsen, W., Leyhausen, G. (1997). Biological aspects of root canal filling materials – histocompatibility, cytotoxicity, and mutagenicity. Clinical Oral Investigations, 1(1), 5-11. Granchi, D., Stea, S., Ciapetti, G., Cavedagna, D., Stea, S., Pizzoferrato, A. (1995). Endodontic cements induce alterations in the cell cycle of in vitro cultured osteoblasts. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 79(3), 359-366. Grossman, L. I. (1958). An improved root canal cement. The Journal of the American Dental Association, 56(3), 381-385. Guo, C., Yang, M., Jing, L., Wang, J., Yu, Y., Li, Y., . . . Sun, Z. (2016). Amorphous silica nanoparticles trigger vascular endothelial cell injury through apoptosis and autophagy via reactive oxygen species-mediated MAPK/Bcl-2 and PI3K/Akt/mTOR signaling. Int J Nanomedicine, 11, 5257-5276. Huang, F.-M., Chang, Y.-C. (2007). Induction of heme oxygenase-1 expression by root canal sealers in human gingival fibroblasts is augmented by oxidative stress. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 80B(2), 499-504. Huang, F.-M., Chou, M.-Y., Chang, Y.-C. (2003). Induction of cyclooxygenase-2 mRNA and protein expression by epoxy resin and zinc oxide-eugenol based root canal sealers in human osteoblastic cells. Biomaterials, 24(11), 1869-1875. Huang, F.-M., Tsai, C.-H., Yang, S.-F., Chang, Y.-C. (2005). Induction of Interleukin-6 and Interleukin-8 Gene Expression by Root Canal Sealers in Human Osteoblastic Cells. Journal of Endodontics, 31(9), 679-683. Huang, F. M., Tai, K. W., Chou, M. Y., Chang, Y. C. (2002). Cytotoxicity of resin‐, zinc oxide–eugenol‐, and calcium hydroxide‐based root canal sealers on human periodontal ligament cells and permanent V79 cells. International Endodontic Journal, 35(2), 153-158. Jeong, S. H., Kim, H. J., Ryu, H. J., Ryu, W. I., Park, Y. H., Bae, H. C., . . . Son, S. W. (2013). ZnO nanoparticles induce TNF-alpha expression via ROS-ERK-Egr-1 pathway in human keratinocytes. J Dermatol Sci, 72(3), 263-273. Kasraei, S., Sami, L., Hendi, S., Alikhani, M. Y., Rezaei-Soufi, L., Khamverdi, Z. (2014). Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus. Restor Dent Endod, 39(2), 109-114. Keller, A. A., McFerran, S., Lazareva, A., Suh, S. (2013). Global life cycle releases of engineered nanomaterials. Journal of Nanoparticle Research, 15(6), 1692. Kishen, A., Shi, Z., Shrestha, A., Neoh, K. G. (2008). An investigation on the antibacterial and antibiofilm efficacy of cationic nanoparticulates for root canal disinfection. J Endod, 34(12), 1515-1520. Kumar, H., Kim, I. S., More, S. V., Kim, B. W., Choi, D. K. (2014). Natural product-derived pharmacological modulators of Nrf2/ARE pathway for chronic diseases. Nat Prod Rep, 31(1), 109-139. Lin, W., Xu, Y., Huang, C.-C., Ma, Y., Shannon, K. B., Chen, D.-R., Huang, Y.-W. (2008). Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells. Journal of Nanoparticle Research, 11(1), 25-39. Mao, Z., Zhou, X., Gao, C. (2013). Influence of structure and properties of colloidal biomaterials on cellular uptake and cell functions. Biomaterials Science, 1(9), 896. Meyer, K., Rajanahalli, P., Ahamed, M., Rowe, J. J., Hong, Y. (2011). ZnO nanoparticles induce apoptosis in human dermal fibroblasts via p53 and p38 pathways. Toxicol In Vitro, 25(8), 1721-1726. Moezzi, A., McDonagh, A. M., Cortie, M. B. (2012). Zinc oxide particles: Synthesis, properties and applications. Chemical Engineering Journal, 185-186, 1-22. Moos, P. J., Chung, K., Woessner, D., Honeggar, M., Cutler, N. S., Veranth, J. M. (2010). ZnO particulate matter requires cell contact for toxicity in human colon cancer cells. Chemical research in toxicology, 23(4), 733-739. Murphy, G., Reynolds, J. J. (1985). Current views of collagen degeradation. Progress towards understanding the resorption of connective tissues. Bioessays, 2(2), 55-60. Mutoh, N., Tani-Ishii, N. (2011). A biocompatible model for evaluation of the responses of rat periapical tissue to a new zinc oxide-eugenol sealer. Dental Materials Journal, 30(2), 176-182. Nair, S., Sasidharan, A., Divya Rani, V. V., Menon, D., Nair, S., Manzoor, K., Raina, S. (2009). Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells. J Mater Sci Mater Med, 20 Suppl 1, S235-241. PAUTKE, C., SCHIEKER, M., TISCHER, T., KOLK, A., NETH, P., MUTSCHLER, W., MILZ, S. (2004). Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer research, 24(6), 3743-3748. Perez de Obanos, M. P., Lopez-Zabalza, M. J., Arriazu, E., Modol, T., Prieto, J., Herraiz, M. T., Iraburu, M. J. (2007). Reactive oxygen species (ROS) mediate the effects of leucine on translation regulation and type I collagen production in hepatic stellate cells. Biochim Biophys Acta, 1773(11), 1681-1688. Podhorecka, M., Skladanowski, A., Bozko, P. (2010). H2AX Phosphorylation: Its Role in DNA Damage Response and Cancer Therapy. J Nucleic Acids, 2010. Pollycove, M., Feinendegen, L. E. (2003). Radiation-induced versus endogenous DNA damage: possible effect of inducible protective responses in mitigating endogenous damage. Human experimental toxicology, 22(6), 290-306. Sahu, D., Kannan, G. M., Vijayaraghavan, R. (2014). Size-dependent effect of zinc oxide on toxicity and inflammatory potential of human monocytes. J Toxicol Environ Health A, 77(4), 177-191. Shrestha, A., Shi, Z., Neoh, K. G., Kishen, A. (2010). Nanoparticulates for antibiofilm treatment and effect of aging on its antibacterial activity. J Endod, 36(6), 1030-1035. Singh, N., Manshian, B., Jenkins, G. J., Griffiths, S. M., Williams, P. M., Maffeis, T. G., . . . Doak, S. H. (2009). NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterials, 30(23-24), 3891-3914. Stone, K., Pryor, W. A. (1994). Cigarettes: then and now. The Journal of laboratory and clinical medicine, 124(3), 313. Suh, K. S., Lee, Y. S., Seo, S. H., Kim, Y. S., Choi, E. M. (2013). Effect of zinc oxide nanoparticles on the function of MC3T3-E1 osteoblastic cells. Biol Trace Elem Res, 155(2), 287-294. Tavassoli Hojati, S., Alaghemand, H., Hamze, F., Ahmadian Babaki, F., Rajab-Nia, R., Rezvani, M. B., . . . Atai, M. (2013). Antibacterial, physical and mechanical properties of flowable resin composites containing zinc oxide nanoparticles. Dent Mater, 29(5), 495-505. Van Landuyt, K. L., Geebelen, B., Shehata, M., Furche, S. L., Durner, J., Van Meerbeek, B., . . . Reichl, F. X. (2012). No evidence for DNA double-strand breaks caused by endodontic sealers. J Endod, 38(5), 636-641. Versiani, M. A., Abi Rached-Junior, F. J., Kishen, A., Pecora, J. D., Silva-Sousa, Y. T., de Sousa-Neto, M. D. (2016). Zinc Oxide Nanoparticles Enhance Physicochemical Characteristics of Grossman Sealer. J Endod, 42(12), 1804-1810. Xia, T., Kovochich, M., Liong, M., Madler, L., Gilbert, B., Shi, H., . . . Nel, A. E. (2008). Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano, 2(10), 2121-2134. Yameen, B., Choi, W. I., Vilos, C., Swami, A., Shi, J., Farokhzad, O. C. (2014). Insight into nanoparticle cellular uptake and intracellular targeting. J Control Release, 190, 485-499. Yu, K. N., Yoon, T. J., Minai-Tehrani, A., Kim, J. E., Park, S. J., Jeong, M. S., . . . Cho, M. H. (2013). Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation. Toxicol In Vitro, 27(4), 1187-1195. Yuan, L., Wang, Y., Wang, J., Xiao, H., Liu, X. (2014). Additive effect of zinc oxide nanoparticles and isoorientin on apoptosis in human hepatoma cell line. Toxicol Lett, 225(2), 294-304. Zhang, Z., Leonard, S. S., Huang, C., Vallyathan, V., Castranova, V., Shi, X. (2003). Role of reactive oxygen species and MAPKs in vanadate-induced G2/M phase arrest. Free Radical Biology and Medicine, 34(10), 1333-1342. Zhuang, S., Yan, Y., Daubert, R. A., Han, J., Schnellmann, R. G. (2007). ERK promotes hydrogen peroxide-induced apoptosis through caspase-3 activation and inhibition of Akt in renal epithelial cells. Am J Physiol Renal Physiol, 292(1), F440-447. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20650 | - |
dc.description.abstract | 實驗目的: 氧化鋅是根管封填劑的主要成分之一。本篇研究的目的主要是在比較不同劑量之市售氧化鋅丁香油酚基底的根管充填劑粉末與微米及奈米等級的氧化鋅顆粒在MG-63類骨細胞中對細胞週期和活性氧產生的影響。 實驗方法: 我們先在掃描電子顯微鏡下觀察藥物粉末的外觀。並將MG-63類骨細胞暴露在不同濃度(0-100μg/ ml)的市售氧化鋅丁香油酚基底根管充填劑 (Roth,canals),微米氧化鋅以及奈米氧化鋅24小時。於光學顯微鏡下觀察加藥24小時後MG-63細胞形態變化。並以反轉錄聚合酶連鎖反應(RT-PCR)西方墨點法(Western Blot)和免疫螢光法 (Immunofluorescence) 探討藥物對MG-63類骨細胞細胞週期,抗氧化反應和第一型膠原蛋白表現的影響。 實驗結果: 市售氧化鋅丁香油酚基底根管充填劑 (Roth®、canals®),微米氧化鋅以及奈米氧化鋅在抑制細胞增殖,細胞週期相關酵素以及抗氧化酵素的表現均類似。在光學顯微鏡下發現,當藥物濃度增加時,細胞數量有顯著的下降。而在反轉錄聚合酶連鎖反應(RT-PCR)西方墨點法(Western Blot)和免疫螢光法 (Immunofluorescence)的檢測中,我們發現cdc25c、cdc2、cycin b1表現量下降而GADD45α 表現量會增加,表示細胞週期停滯在G2 / M期。第一型血紅素氧化酶(HO-1)的表現量也隨著試劑濃度升高而增加。然而,p-Nrf2的表現量卻是減少,這可能表示HO-1的表現並不經由Nrf2途徑的調節。 p-p38和p-ERK的升高和p-Akt的下降也顯示她們在細胞功能的調節扮演重要的角色。 結論: 市售氧化鋅丁香油酚基底根管充填劑 (Roth®、canals®),微米氧化鋅以及奈米氧化鋅對於抑制細胞增殖,細胞週期相關酵素以及抗氧化酵素的表現均類似,它們使MG-63類骨細胞出現細胞週期停滯在G2 / M期和HO-1的表現量增加,其可能與p-p38和p-ERK路徑有關。 | zh_TW |
dc.description.abstract | Aim: Zinc oxide is one of the main ingredient of root canal sealer. The purpose of the study focus on the effect of commercial ZOE root canal sealer powder and different size of Zinc oxide particles in the cell cycle and Reactive Oxygen Species production of MG63 osteoblast-like-cell. Materials and methods: MG63 osteoblast-like-cell were treated with Canals, Roth, micro-ZnO and nano-ZnO in different concentration (10-100 µg/ml) for 24 hours. Canals®, Roth®, micro-ZnO and nano-ZnO were observed under scanning electron microscope. The MG-63 cell morphology was observed under optical microscope. Reverse Transcription Polymerase Chain Reaction) (RT-PCR), Western Blot and Immunofluorescence were used to detect the effect of reagents on cell-cycle regulatory, antioxidant responses and collagen-1 expression. Results: All reagents inhibited cell proliferation in a dose-dependent manner. The decrease of cdc25c, cdc2, cycin b1 and the increase of GADD45α expression suggest the cell cycle arrest at G2/M phase after treatment. Antioxidant enzyme: heme oxygenase-1(HO-1) also expression as the reagents concentration rise. However, the p-Nrf2 expression is decrease which may suggest the activation of HO-1 may not regulated through Nrf2 pathway. The elevation of p-p38 and p-ERK and decline of p-Akt may also play a role in regulation cell functions. Conclusions: Canals®, Roth®, micro-ZnO and nano-ZnO have the similarly effect on inhibited cell proliferation as the concentration rise. They also induced cell cycle arrest at G2/M phase and HO-1 expression which may have relation with p-p38 and p-ERK pathway. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T02:57:01Z (GMT). No. of bitstreams: 1 ntu-106-R03422005-1.pdf: 3872149 bytes, checksum: 9f89fb983e44f6ca9c30c8526951f389 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 中文摘要 ii Abstract iv Table of Contents vi Figures viii Tables xii Abbreviations list xiii Chapter 1 Introduction 1 1.1 Root canals filling materials 1 1.1.1 Classification 1 1.1.2 The biocompatibility of root canal sealers 2 1.2 Zinc oxide particles 3 1.2.1 Zinc oxide particles 3 1.2.2 Cellular uptake particles 4 1.2.3 The mechanisms of particles induced cytotoxicity 6 1.2.4 The biocompatibility comparison between nano-ZnO and micro-ZnO 7 1.2.5 The application of nano-ZnO in dental materials 8 1.3 Reactive oxygen species (ROS) 9 1.4 Cell cycle control 11 1.4.1 Cell cycle regulatory 12 1.4.2 Cell cycle checkpoint 13 1.4.3 DNA damage repair 14 1.4.4 ROS and cell cycle 15 1.5 MG-63 osteoblast-like cell 16 Chapter 2 The Purpose of the Study 18 Chapter 3 Materials and Methods 19 3.1 Materials 19 3.2 Particles observation under SEM 21 3.3 Culture of MG-63 osteoblast-like cell 21 3.4 Reverse transcription-Polymerase Chain Reaction (RT-PCR) 22 3.4.1 Total RNA Isolation 22 3.4.2 RNA quantification 24 3.4.3 Reverse transcription 24 3.4.4 Polymerase chain reaction (PCR) 25 3.4.5 Gel Electrophoresis 26 3.5 Western Blot 27 3.5.1 Protein extraction 27 3.5.2 Protein quantification 28 3.5.3 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) 29 3.5.4 Western Blot 30 3.6 Immunofluorescence 31 3.7 Statistical analysis 34 Chapter 4 Results 35 4.1 Reagents morphology observation under SEM 35 4.2 Cell morphology observation 35 4.3 Effect of reagents on cell cycle progression 36 4.4 Effect of reagents on the expression of antioxidant -related genes and enzyme 37 4.5 Effect of reagents on the expression of MAPK and p-Akt 37 4.6 Effect of reagents on the expression of collagen-1 gene and collagen-1 α 1 protein 38 4.7 Effect of reagents on the expression of p-ATM, p-ATR, p-Chk1, p-Chk2 38 Chapter 5 Discussion 39 5.1 Cell morphological change and inhibition of proliferation 39 5.2 Effect on cell cycle regulation 40 5.3 Effect on antioxidant response (Nrf2, p-Nrf2, HO-1) 42 5.4 Effect on p-Akt and MAPK pathway 43 5.5 Effect on collagen-1 44 Chapter 6 Conclusion 46 References 47 Appendices 55 | |
dc.language.iso | en | |
dc.title | 不同根管封填劑和氧化鋅顆粒對MG-63類骨細胞之細胞週期及活性氧產生的影響及其機制 | zh_TW |
dc.title | The effect and mechanism of root canal sealers and Zinc oxide particles on cell cycle control and reactive oxygen species production of MG-63 osteoblast-like cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李勝揚,楊淑芬,楊台鴻,張美姬 | |
dc.subject.keyword | MG-63類骨細胞,氧化鋅,G2 / M期停滯,第一型血紅素氧化?,氧化鋅丁香油酚根管充填劑, | zh_TW |
dc.subject.keyword | MG-63 osteoblast-like cells,Zinc oxide-eugenol-based root canal,Zinc oxide,cell cycle G2/M arrest,Hemo oxygenase-1, | en |
dc.relation.page | 77 | |
dc.identifier.doi | 10.6342/NTU201702360 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2017-08-01 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
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