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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 金洛仁(Laurent Zimmerli) | |
| dc.contributor.author | Yi-Chun Huang | en |
| dc.contributor.author | 黃義竣 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:56:31Z | - |
| dc.date.copyright | 2017-08-04 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-02 | |
| dc.identifier.citation | Acharya, B.R., Raina, S., Maqbool, S.B., Jagadeeswaran, G., Mosher, S.L., Appel, H.M., Schultz, J.C., Klessig, D.F., and Raina, R. (2007). Overexpression of CRK13, an Arabidopsis cysteine-rich receptor-like kinase, results in enhanced resistance to Pseudomonas syringae. Plant J. 50, 488–499.
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Nature 428, 764–767. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20639 | - |
| dc.description.abstract | 訊息胜肽調控植物的生長發育以及多種生理反應，如根部組織生長、葉形調控、防禦反應、癒合組織生長、頂端分生組織構型組成等。然而訊息胜肽如何調控植物組織之發育仍有許多未知，本研究透過比較植物在不同胜肽處理後之發育情形，篩選出阿拉伯芥CUE胜肽家族中的KottoiA/B胜肽，實驗顯示KottoiA/B會透過不同受體激酶調控葉部stomatal lineage stem cell數量以及根部之維管束發育。植物不僅能透過受體激酶感受內在訊號，也能藉由受體激酶接收環境訊息做出反應，本研究發現，富含半胱胺酸受體激酶18與絲裂原活化蛋白質激酶6能夠維持模式誘發免疫反應，使其不被菜籽類固醇訊號傳遞路徑抑制。透過對植物轉錄調控以及表型的分析發現，半胱胺酸重複受體激酶18及有絲分裂活化蛋白質激酶6會負向調控菜籽類固醇訊號傳遞路徑。因此推論半胱胺酸重複受體激酶18及有絲分裂活化蛋白質激酶6可能參與在模式誘發免疫反應抑制菜籽類固醇訊號傳遞路徑中。 | zh_TW |
| dc.description.abstract | Peptide signaling molecules are increasingly known to have important and diverse roles in regulation of developmental and physiological processes in plants. Here, we found that the peptide KottoiA/B, belonging to the CLAVATA3/ESR-unlike (CUE) family, regulates two different developmental processes, namely stomatal lineage and xylem developments in Arabidopsis thaliana. We also found that Kottoi A/B is perceived by two distinct receptor-like kinases (RLKs) systems in different tissues regulating distinct developmental processes. Plant cell use RLKs as transmembrane sensors to perceive and process peptide signals. RLKs not only sense endogenous signals but also exogenous signals. Our study revealed a novel RLK, CCR18 and it upstream MPK6 maintain the activation of PTI. Notably, by transcriptional and phenotype analyses, we show that CCR18 and MPK6 negatively regulate BRs signaling and positively regulate flg22-mediated growth inhibition. Therefore, CRK18 and MPK6 may be necessary for PTI-mediated reduction of BR-signaling. Our results further provide insight into understanding the fine-tune of plant immunity and may have potential for agricultural application. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:56:31Z (GMT). No. of bitstreams: 1 ntu-106-R04b42010-1.pdf: 12502684 bytes, checksum: 7c4657c95ea7135a8547f07c33026fb6 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | Contents
致謝 II Abstract III 摘要 IV Contents V Part 1. Abstract 1 第一部分摘要 2 Introduction 3 Materials and Methods 7 Biological materials and growth conditions 7 Chemical Treatments 7 RNA extraction and gene expression 8 Hypocotyl and Root length measurements 9 Results 9 CRK18 does not directly modulate BR signaling 10 CRK18 modulates the BR response in a flg22 -dependent manner 11 MPK6 is upstream of CRK18 in the flg22-mediated repression of BR signaling 12 MPK6 is involved in BR biosynthesis pathway in a BL-dependent manner 13 Discussion 15 Conclusions and Future Perspectives 18 Figures 19 Figure 1. BR Responses in crk18 Mutants after BL treatment 19 Figure 2. Characterization of crk18 mutants after flg22 treatment 21 Figure 3 Characterization of mpk6 mutant after flg22 treatment 23 Figure 4. mpk6 mutant is not responsive to flg22 treatment 25 Figure 5. Phenotype of mpk6 mutant after BL treatment 26 Figure 6. Characterization of mpk6 mutant after BL treatment 27 Figure S1. Characterization of CRK18 mutants and OE Lines 29 Table S1 Primer list in this study 30 Part 2. Abstract 31 第二部分摘要 32 Introduction 33 Materials and Methods 36 Plant materials and growth conditions. 36 Microscopy and quantitative analysis of the epidermis. 36 Peptide treatment on plants 38 CRISPR 38 CLE peptide treatment on plants 39 Peptide synthesis 39 VISUAL 39 Results 41 KottoiA and KottoiB are preferentially expressed in stomatal lineage precursor cells and guard cells 41 kottoiB mutants show increased cell proliferation during stomatal development 42 KottoiA/B is perceived through a novel receptor 43 Identification of KottoiB receptor HSL1 in stomatal development 44 KottoiA/B negatively regulates metaxylem formation through BAM receptors 47 Discussion 49 Conclusions and Future Perspectives 51 Figures 52 Fig. 1. Promoter activities of KottoiB and KottoiA. 52 Fig. 2. The KottoiA/B peptide negatively regulates the formation of stomatal precursor cells. 53 Fig. 3. Development of epidermal cells in the er erl1 erl2 triple mutant is sensitive to KottoiA/B. 55 Fig. 4. Effects of mutations in BAMs/CLV1 class LRR RLK genes on epidermal cell development and root elongation. 56 Fig. 5. The HSL1 receptor-like kinase is indispensable for the KottoiA/B action for regulation of stomatal development. 58 Fig. 6. KottoiA/B negatively regulates metaxylem formation through BAM receptors. 60 Fig. 7. Hypothetical model. 61 Supplementary Data 62 Fig. S1. KottoiA/B did not decrease epidermal cell number in cotyledons in spch. Number of epidermal cells in adaxial side of cotyledons were counted (n= 5). Data done by Dr.Pingping Qian. 62 Table S1. A gene and mutant list of LRR-RLK XI family used for KottoiA/B receptor screening 66 Table S2 Primer list in this study. 67 References 68 | |
| dc.language.iso | en | |
| dc.title | 阿拉伯芥富含半胱胺酸受體激酶18及絲裂原活化蛋白質激酶6在抗病反應抑制菜籽類固醇路徑及KottoiA/B胜肽透過不同受體調控植物氣孔和維管束之發育之功能性分析 | zh_TW |
| dc.title | Functional Characterization of CYSTEINE-RICH RECEPTOR-LIKE Arabidopsis PTI repression of BR signaling And KottoiA/B peptide regulates development of stomata and vasculature through distinct receptors | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 謝旭亮(Hsu-Liang Hsieh),林乃君(Nai-Chun Lin),鄭貽生(Yi-Sheng Cheng),張英?(Ing-Feng Chang) | |
| dc.subject.keyword | 阿拉伯芥,免疫反應,賀爾蒙,生物逆境, | zh_TW |
| dc.subject.keyword | arabidopsis,immunity,mpk6,pti, | en |
| dc.relation.page | 75 | |
| dc.identifier.doi | 10.6342/NTU201702431 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2017-08-03 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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