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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王愛玉 | |
| dc.contributor.author | Jen-Shuang Wang | en |
| dc.contributor.author | 王人雙 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:26:39Z | - |
| dc.date.copyright | 2015-08-25 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-18 | |
| dc.identifier.citation | Alexandrov, N.N., Brover, V.V., Freidin, S., Troukhan, M.E., Tatarinova, T.V., Zhang, H., Swaller, T.J., Lu, Y.P., Bouck, J., Flavell, R.B., Feldmann, K.A. (2009) Insights into corn genes derived from large-scale cDNA sequencing. Plant Mol Biol, 69(1-2), 179-194. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (1987) Current Protocols in Molecular Biology, John Wiley Sons, Inc, New York. Aviv, D.H., Rusterucci, C., Holt, B.F., Dietrich, R.A., Parker, J.E., Dangl, J.L. (2002) Runaway cell death, but not basal disease resistance, in lsd1 is SA‐and NIM1NPR1‐dependent. The Plant Journal, 29(3), 381-391. Bhatti, K.B., Ahmed, N., Shah, A., Iqbal, M., Iqbal, T., Jiahe, W. (2011) Transgenic tobacco with rice zinc-finger gene OsLOL2 exhibits an enhanced resistance against bacterial-wilt. Australasian Plant Pathology, 40, 133-140. Bhatti, K.H., Xu, C., Wu, J., He, C. (2008) Overexpression of rice OsLOL2 gene confers disease resistance in tobacco to Pseudomonas syringae pv. tabaci. Progress in Natural Science, 18(7), 807-812. Chai, T., Zhou, J., Liu, J., Xing, D. (2015) LSD1 and HY5 antagonistically regulate red light induced-programmed cell death in Arabidopsis. Front Plant Sci, 6, 292. Dietrich, R.A., Delaney, T.P., Uknes, S.J., Ward, E.R., Ryals, J.A., Dangl, J.L. (1994) Arabidopsis mutants simulating disease resistance response. Cell, 77, 565-577. Dietrich, R.A., Richberg, M.H., Schmidt, R., Dean, C., Dangl, J.L. (1997) A novel zinc finger protein is encoded by the Arabidopsis LSD1 gene and functions as a negative regulator of plant cell death. Cell, 88, 685-694. Epple, P., Mack, A.A., Morris, V.R., Dangl, J.L. (2003) Antagonistic control of oxidative stress-induced cell death in Arabidopsis by two related, plant-specific zinc finger proteins. PNAS, 100(11), 6831-6836. Guo, J., Bai, P., Yang, Q., Liu, F., Wang, X., Huang, L., Kang, Z. (2013) Wheat zinc finger protein TaLSD1, a negative regulator of programmed cell death, is involved in wheat resistance against stripe rust fungus. Plant Physiol Biochemistry, 71, 164-172. He, S., Huang, K., Zhang, X., Yu, X., Huang, P., An, C. (2011) The LSD1-type zinc finger motifs of Pisum sativa LSD1 are a novel nuclear localization signal and interact with importin alpha. PLOS one, 6(7), e22131. Huang, X., Li, Y., Zhang, X., Zuo, J., Yang, S. (2010) The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death. New Phytol, 187(2), 301-312. Jabs, T., Dietrich, R.A., Dangl, J.L. (1996) Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide. Science, 273, 1853-1856. Kaminaka, H., Nake, C., Epple, P., Dittgen, J., Schutze, K., Chaban, C., Holt, B.F., Merkle, T., Schafer, E., Harter, K., Dangl, J.L. (2006) bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection. 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(2004) LESION SIMULATING DISEASE 1 is required for acclimation to conditions that promote excess excitation energy. Plant Physiology, 136(1), 2818-2830. Muhlenbock, P., Plaszczyca, M., Plaszczyca, M., Mellerowicz, E., Karpinski, S. (2007) Lysigenous aerenchyma formation in Arabidopsis is controlled by LESION SIMULATING DISEASE1. Plant Cell, 19(11), 3819-3830. Muhlenbock, P., Szechynska-Hebda, M., Plaszczyca, M., Baudo, M., Mullineaux, P.M., Parker, J.E., Karpinska, B., Karpinski, S. (2008) Chloroplast Signaling and LESION SIMULATING DISEASE1 Regulate Crosstalk between Light Acclimation and Immunity in Arabidopsis. Plant Cell, 20(9), 2339-2356. Ouwerkerk, P.B. and Meijer, A.H. (2011) Yeast one-hybrid screens for detection of transcription factor DNA interactions. Methods in Molecular Biology, 678, 211-227. Pyvovarenko, T. and Lopato, S. (2011) Isolation of plant transcription factors using a yeast one-hybrid system. Methods in Molecular Biology, 754, 45-66. 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Wu, J., Zhu, C., Pang, J., Zhang, X., Yang, C., Xia, G., Tian, Y., He, C. (2014) OsLOL1, a C2C2-type zinc finger protein, interacts with OsbZIP58 to promote seed germination through the modulation of gibberellin biosynthesis in Oryza sativa. Plant J, 80(6), 1118-1130. Xu, C. and He, C. (2007) The rice OsLOL2 gene encodes a zinc finger protein involved in rice growth and disease resistance. Mol Genet Genomics, 278(1), 85-94. Yeh, S.H., Lin, C.S., Wu, F.H., Wang, A.Y. (2011) Analysis of the expression of BohLOL1, which encodes an LSD1-like zinc finger protein in Bambusa oldhamii. Planta, 234(6), 1179-1189. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19911 | - |
| dc.description.abstract | BohLOL1 (Bambusa oldhami LSD-One-Like 1) 基因轉譯出的蛋白質具有 DNA 結合能力且存在於細胞核中，其胺基酸序列具有三個 zf-LSD1 (zinc finger-Lesions Simulating Disease resistance 1) domains。在過去研究中發現於快速生長時期的綠竹筍中，BohLOL1 mRNA 含量大幅提升，此外以 auxins、cytokinins 或病原菌處理竹苗後，mRNA 含量亦會大幅提升。本研究以 PCR選殖出 BohLOL1 的genomic DNA，將其與 cDNA 序列比對後，顯示 BohLOL1 基因結構具有 5 個 exons、4 個 introns。然而，將 BohLOL1 基因與其他植物 LSD 及 LOL 相關基因之結構比較後，推測 BohLOL1 可能在轉譯起始點上游仍有一個 exon 存在。此外，利用 genome walking 法選殖 BohLOL1 轉譯起始點之上游序列，並預測此序列上之順式調控因子 (cis-elements)，發現許多與植物荷爾蒙或逆境相關的因子，且在孟宗竹 (Phyllostachys edulis) 同源基因 PH01001063G0080 (稱之為 PeLOL1) 的基因調控序列中，也有預測到相同的順式調控因子。BohLOL1 與 PeLOL1在核苷酸及胺基酸序列之同質性高，兩者的基因結構及調控序列上之順式調控因子亦具保留性，顯示 BohLOL1 與 PeLOL1 為直系同源基因，並且在植物生長與防禦的過程中具有相似的功能。 | zh_TW |
| dc.description.abstract | BohLOL1 (Bambusa oldhami LSD-One-Like 1) encodes a nuclear DNA-binding protein containing three zf-LSD1 (zinc finger-Lesions Stimulating Disease resistance 1) domains. This gene has been shown to be upregulated in bamboo shoots with higher rates of culm elongation, and also upregulated by auxins, cytokinin, and pathogen infection. In this study, BohLOL1 genomic DNA was cloned using PCR. Comparing the genomic DNA sequence of BohLOL1 with its cDNA sequence revealed that BohLOL1 gene had 5 exons and 4 introns. However, the result of sequence alignment of BohLOL1 with LSD1- and LOL1- related genes from other plants suggested that BohLOL1 had another exon upstream the translation start site. Genome walking was performed to clone the upstream sequence. Many kinds of putative regulatory cis-elements were found in the sequence upstream the predicted transcription initiation site. Some cis-elements were related to pytohormones or stresses, and these cis-elements could also be found in the promoter region of a homologous gene (PH01001063G0080, designated PeLOL1) in moso bamboo (Phyllostachys edulis). BohLOL1 and PeLOL1 show high nucleotide and amino acid sequence identities. Conservation of structural gene sequence and cis-elements in their regulatory regions suggested that BohLOL1 and PeLOL1 were orthologs and had similar biological functions in plant growth or disease resistance. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:26:39Z (GMT). No. of bitstreams: 1 ntu-104-R02B22007-1.pdf: 2165263 bytes, checksum: 84dc96ee6d4bb99c2188c02644c4a538 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 目錄 i 縮寫表 iv 摘要 v Abstract vi 第一章 研究背景 1 1.1 緣起 1 1.2 LSD1 及 LOL1 之相關研究 1 1.3 BohLOL1 之基因表現與功能 2 1.4 本實驗之目的與策略 3 第二章 材料與方法 4 2.1 實驗材料 4 2.1.1 植物材料 4 2.1.2 菌種 4 2.1.2.1 大腸桿菌 (Escherichia coli) 4 2.1.2.2 酵母菌 (Saccharomyces cerevisiae) 4 2.1.3 質體 4 2.2 藥品與儀器 5 2.2.1 實驗藥品 5 2.2.2 儀器 5 2.3 實驗方法 5 2.3.1 DNA之抽取、檢定與分析方法 5 2.3.1.1 綠竹筍基因體 DNA 之萃取 5 2.3.1.2 大腸桿菌質體 DNA 之小量萃取 6 2.3.1.3 瓊脂糖膠體電泳 6 2.3.1.4 聚合酶鏈鎖反應 (Polymerase Chain Reaction, PCR) 7 2.3.1.5 PCR 產物之 A-tailing 7 2.3.1.6 DNA 片段之純化 8 2.3.1.7 DNA 之限制酶截切 8 2.3.1.8 DNA 片斷之粘接 (Ligation) 8 2.3.1.9 酵母菌基因體 DNA 之小量萃取 8 2.3.1.10 酵母菌質體 DNA 之粗萃取 9 2.3.1.11 基因結構分析 9 2.3.2 質體 DNA 之轉形 9 2.3.2.1 以大腸桿菌為宿主 9 2.3.2.2 以酵母菌為宿主 10 2.3.2.3 轉形株之篩選 10 2.3.3 BohLOL1 上游調控序列之選殖與序列分析 11 2.3.3.1 上游調控序列之選殖 11 2.3.3.2 啟動子序列分析 12 2.3.4 RNA 之抽取、檢定與純化 12 2.3.4.1 綠竹筍 total RNA 之萃取 (Trizol 法) 12 2.3.4.2 甲醛瓊脂糖膠體電泳 13 2.3.4.3 Poly (A)+ RNA之純化 13 2.3.5 建立綠竹筍 cDNA library 14 2.3.5.1 cDNA 之合成 14 2.3.5.2 cDNA 之純化 15 2.3.5.3 建構 cDNA library 15 2.3.6 酵母菌單雜交系統 (Yeast one-hybrid system, Y1H) 15 2.3.6.1 建立釣餌質體及檢定 15 2.3.6.2 測試 3-AT (3-Amino-1,2,4-triazole) 之使用濃度 17 2.3.6.3 cDNA library 之篩選 17 2.3.6.4 確認 cDNA library 篩選結果之正確性 17 第三章 結果與討論 18 3.1 BohLOL1基因選殖 18 3.2 BohLOL1 與孟宗竹同源基因之比較 18 3.3 不同物種 LOL1 及 LSD1 基因結構之比較 19 3.4 BohLOL1上游調控序列之選殖 19 3.5 基因調控序列分析 20 3.6 酵母菌單雜交系統建構與篩選 21 第四章 結論與未來展望 24 參考文獻 25 圖與表 27 | |
| dc.language.iso | zh-TW | |
| dc.title | 綠竹 BohLOL1 基因之選殖與分析 | zh_TW |
| dc.title | Cloning and Analysis of LSD-one-like 1 Gene from Bambusa oldhamii | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 宋賢一,廖憶純,張麗冠,楊健志 | |
| dc.subject.keyword | 竹生長發育,綠竹,抗病性,順式調控因子, | zh_TW |
| dc.subject.keyword | BohLOL1 (Bambusa oldhami LSD-One-Like 1),bamboo growth,disease resistance,cis-elements, | en |
| dc.relation.page | 59 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2015-08-18 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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