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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 徐善慧(Shan-hui Hsu) | |
dc.contributor.author | Hsin-Hua Lin | en |
dc.contributor.author | 林欣樺 | zh_TW |
dc.date.accessioned | 2021-06-08T02:12:24Z | - |
dc.date.copyright | 2016-02-24 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-01-18 | |
dc.identifier.citation | [1] Bak D. Rapid prototyping or rapid production 3D printing processes move industry towards the latter. Assembly Autom. 2003;23:340-5.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19668 | - |
dc.description.abstract | 3D列印製備組織工程客製化支架是一項極具潛力的加工技術。其中以水膠材料作為生物列印墨水結合細胞列印更可以提供基礎研究及未來醫學治療生物平台。在本研究中,製備三種水性生物可降解聚己內酯(poly(ε-caprolactone), PCL)基底的聚胺酯(polyurethane, PU)奈米水分散液,其PU流體-膠體(sol-gel)的相轉變會受到溫度及電解質影響,以動態光散射(dynamic light scattering, DLS)、小角度X-ray散射(small angle X-ray scattering, SAXS)、X-ray繞射(X-ray diffraction, XRD)及流變學(rheology)等分析方法探討凝膠行為及成膠機制。PU軟鏈段中由20 mol% L型聚乳酸二元醇(poly (L-lactide) , PLLA diol)或DL型聚乳酸二元醇(poly (D,L-lactide), PDLLA diol)及80 mol% PCL二元醇所組成之奈米分散液可在高於37°C時形成緊密堆積的結構。PLLA-PCL軟鏈段組成之PU對溫度提升較有響應性,而PDLLA-PCL軟鏈段組成的PU對額外添加的電解質較為敏感。具有溫感性的PU分散液可於3分鐘內成膠並在30分鐘後達到膠體模數6-8 kPa。此PU水膠可藉由預熱及均勻混合細胞製備生物列印墨水,並於37°C平台3D列印。另外,更進一步引入大豆分離蛋白(soy protein isolate, SPI)製備PU混合膠(PU/SPI hybrid)以增加列印過程中的結構堆疊完整性。PU/SPI混合膠相較於PU有較低的黏度,卻可在37°C更快速的成膠並在1分鐘內達到130 Pa的膠體模數。且PU/SPI hybrid可省去預熱過程直接在室溫(25°C)混入細胞並於37°C直接進行列印。48小時細胞培養在PU/SPI hybrid比純PU膠中有較佳的細胞相容性。此種由SPI與溫度敏感性PU形成的混合膠可能是未來生物3D列印具潛力新型的生物列印墨水。 | zh_TW |
dc.description.abstract | 3D printing technique shows great promises for fabricating customized structural scaffolds for tissue regeneration. Using hydrogel as bioink for cell printing provides a biological platform for basic research and potential medical treatment. In this study, the waterborne dispersions of poly(ε-caprolactone) (PCL)-based biodegradable polyurethane (PU) nanoparticles were prepared. The sol-gel transition of the PU dispersions affected by temperature or electrolytes was examined by dynamic light scattering (DLS), small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and rheology. The dispersion of PU nanoparticles with 20 mol% poly (L-lactide) (PLLA) diol or poly (D,L-lactide) (PDLLA) diol and 80 mol% PCL diol in soft segement composition could form compact packing structure at temperatures ≥37°C. The dispersion of PU with PLLA-PCL soft segment was more responsive to the temperature increase, while that with PDLLA-PCL soft segment was more responsive to the added electrolytes. With thermally-responsive properties, both PU dispersions could gel in 3 min with the gel modulus increased to about 6-8 kPa after 30 min. PU hydrogels were preheated and blended with cells, and the mixture was later printed at 37°C. The hybrid hydrogel of PU and soy protein isolate (PU/SPI hybrid) was further developed to enhance the structural integrity of the cell-laden constructs during deposition. The viscosity of the PU/SPI hybrid was lower than that of PU gel, but could undergo rapid gelation at 37°C with modulus increased to 130 Pa in 1 min. Moreover, the PU/SPI hybrid gel may be directly mixed with cells at room temperature and subsequently printed at 37°C without preheating. Cells cultured in the PU/SPI hybrid gel for 48 h proliferated faster than those in PU gel. The hybrid gel made of thermoresponsive PU and SPI may be a new type of bioink for 3D biopriniting applications. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T02:12:24Z (GMT). No. of bitstreams: 1 ntu-105-R02549044-1.pdf: 8401953 bytes, checksum: 562512fb69367b0cd81151ebd28f5354 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 致謝 i
摘要 ii Abstract iv 目錄 vi 圖目錄 xi 表目錄 xiii 第一章 文獻回顧 1 1.1. 3D列印及3D列印材料 1 1.2. 生物3D列印水膠材料 2 1.3. 天然型水膠材料 3 1.4. 合成型水膠材料 4 1.5. 混合型水膠材料 5 1.6. 聚胺酯 5 1.6.1. 水性聚胺酯 6 1.7. 研究動機 7 第二章 研究方法 8 2.1. 研究架構 8 2.2. DL型聚乳酸二元醇(poly(DL-lactic acid diol), PDLLA diol)或L型聚乳酸二元醇(poly(L-lactic acid diol), PLLA diol)合成與純化 10 2.2.1. PDLLA diol (或PLLA diol)合成 10 2.2.2. PDLLA diol (或PLLA diol)純化 11 2.3. 核磁共振光譜儀末端基法測定PDLLA diol及PLLA diol分子量 11 2.4. 水性生物可降解聚胺酯(PU)軟鏈段熱性質分析 11 2.5. 水性PU合成 12 2.5.1. PU原料與配方設計 12 2.5.2. PU合成 13 2.6. PU奈米粒基礎性質分析 16 2.6.1. 動態光散射(Dynamic light scattering, DLS)分析 16 2.7 小角度X光散射(Small- angle light scattering, SAXS)分析 16 2.8. 溫度敏感性PU sol-gel相轉換動態流變性質分析 17 2.9. X光繞射(X-ray diffraction, XRD)分析 17 2.10. 衰退全反射傅立葉紅外光譜儀(ATR-FTIR) 18 2.11. 鈣離子吸附原子吸收分光光度法(atomic absorption, AA)分析 18 2.12. 組織工程實驗 18 2.12.1. 細胞培養 18 2.12.2. 細胞染色與標定 19 2.12.3. 製備PU水膠及大豆蛋白混合膠與細胞包覆 19 2.12.4. 3D列印參數及支架尺寸 20 2.12.5. 細胞存活率測定 20 2.13. 統計分析 21 第三章 實驗結果 22 3.1. L型聚乳酸二元醇及DL型聚乳酸二元醇核磁共振分析 22 3.2. 水性PU軟鏈段熱性質分析 22 3.3. 水性PU合成及基本特性分析 23 3.4. 溫度敏感性PU奈米粒動態光散射粒徑分析 23 3.5. PU分散液小角度X光散射分析 23 3.6. 電解質敏感性PU奈米粒動態光散射分析 24 3.7. PU分散液動態流變性質分析 25 3.8. PU分散液於電解質中動態流變性質分析 25 3.9. PCL80DL20及SPI混合膠流變性質分析 26 3.10. PU結晶性質X光繞射分析 27 3.11. PU氫鍵作用力衰退式全反射傅立葉紅外光譜分析 27 3.12. PU鈣離子吸附原子吸收分光光度法分析 28 3.13. PU水膠及混合膠細胞包覆3D列印 28 第四章 討論 29 4.1. PU軟鏈段基本性質分析 29 4.1.1. PLLA diol及PDLLA diol核磁共振分析 29 4.1.2. PU軟鏈段熱性質分析 29 4.2. 水性PU奈米粒基礎性質分析 29 4.3. 溫度敏感性PU奈米粒粒徑分析 30 4.4. 電解質敏感性PU奈米粒動態光散射分析 31 4.5. 電解質敏感性PU SAXS分析 31 4.6. PU分散液動態流變性質分析 32 4.7. PU分散液於細胞培養液中動態流變性質分析 33 4.8. PCL80DL20及SPI混合膠流變性質分析 33 4.9. PCL80DL20及SPI混合膠SAXS分析 34 4.10. PU結晶性質X光繞射分析 34 4.11. PU氫鍵作用力衰退式全反射傅立葉紅外光譜分析 35 4.12. PU鈣離子吸附原子吸收分光光度法分析 35 4.13. PU水膠及混合膠細胞包覆3D列印 36 4.14. 未來展望 36 第五章 結論 38 參考文獻 39 | |
dc.language.iso | zh-TW | |
dc.title | 可降解聚胺酯水膠及大豆蛋白混合膠3D生物列印 | zh_TW |
dc.title | Preparation and characterization of biodegradable polyurethane hydrogel and the hybrid gel with soy protein for 3D cell-laden bioprinting | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張振榮,戴念國,謝馥羽 | |
dc.subject.keyword | 細胞3D列印,水膠材料,生物可降解聚胺酯(PU),溫度敏感性,大豆蛋白,混合膠, | zh_TW |
dc.subject.keyword | Cell-laden 3D bioprinting,hydrogel,biodegradable polyurethane,thermoresponsive,soy protein isolate,hybrid gel, | en |
dc.relation.page | 64 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2016-01-18 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
顯示於系所單位: | 高分子科學與工程學研究所 |
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