請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19646完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 孫錦虹(Chin-hung Sun) | |
| dc.contributor.author | Yu-Chien Chen | en |
| dc.contributor.author | 陳宇芊 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:11:10Z | - |
| dc.date.copyright | 2016-02-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-01-26 | |
| dc.identifier.citation | Adam, R. D. (2001). Biology of Giardia lamblia. Clin Microbiol Rev, 14(3), 447-475.
Ankarklev, J., Jerlstrom-Hultqvist, J., Ringqvist, E., Troell, K., & Svard, S. G. (2010). Behind the smile: cell biology and disease mechanisms of Giardia species. Nat Rev Microbiol, 8(6), 413-422. An, X. J., Deng, Z. Y., & Wang, T. (2011). OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB. PLoS One, 6(5), e20327. Baudat, F., Manova, K., Yuen, J. P., Jasin, M., & Keeney, S. (2000). Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11. Mol Cell, 6(5), 989-998. Bekker-Jensen, S., & Mailand, N. (2010). Assembly and function of DNA double-strand break repair foci in mammalian cells. DNA Repair (Amst), 9(12), 1219-1228. Berger, J. M., Gamblin, S. J., Harrison, S. C., & Wang, J. C. (1996). Structure and mechanism of DNA topoisomerase II. Nature, 379(6562), 225-232. Bergerat, A., de Massy, B., Gadelle, D., Varoutas, P. C., Nicolas, A., & Forterre, P. (1997). An atypical topoisomerase II from Archaea with implications for meiotic recombination. Nature, 386(6623), 414-417. Blanco, M. G., Boan, F., Barros, P., Castano, J. G., & Gomez-Marquez, J. (2005). Generation of DNA double-strand breaks by two independent enzymatic activities in nuclear extracts. J Mol Biol, 351(5), 995-1006. Buhler, C., Gadelle, D., Forterre, P., Wang, J. C., & Bergerat, A. (1998). Reconstitution of DNA topoisomerase VI of the thermophilic archaeon Sulfolobus shibatae from subunits separately overexpressed in Escherichia coli. Nucleic Acids Res, 26(22), 5157-5162. Buhler, C., Lebbink, J. H., Bocs, C., Ladenstein, R., & Forterre, P. (2001). DNA topoisomerase VI generates ATP-dependent double-strand breaks with two-nucleotide overhangs. J Biol Chem, 276(40), 37215-37222. Carpenter, M. L., Assaf, Z. J., Gourguechon, S., & Cande, W. Z. (2012). Nuclear inheritance and genetic exchange without meiosis in the binucleate parasite Giardia intestinalis. J Cell Sci, 125(Pt 10), 2523-2532. Carranza, P. G., & Lujan, H. D. (2010). New insights regarding the biology of Giardia lamblia. Microbes Infect, 12(1), 71-80. Chen, Y. H., Su, L. H., & Sun, C. H. (2008). Incomplete nonsense-mediated mRNA decay in Giardia lamblia. Int J Parasitol, 38(11), 1305-1317. Christensen, G. L., Ivanov, I. P., Atkins, J. F., Mielnik, A., Schlegel, P. N., & Carrell, D. T. (2005). Screening the SPO11 and EIF5A2 genes in a population of infertile men. Fertil Steril, 84(3), 758-760. Corbett, K. D., & Berger, J. M. (2004). Structure, molecular mechanisms, and evolutionary relationships in DNA topoisomerases. Annu Rev Biophys Biomol Struct, 33, 95-118. Diaz, R. L., Alcid, A. D., Berger, J. M., & Keeney, S. (2002). Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation. Mol Cell Biol, 22(4), 1106-1115. Ekdahl, K., & Andersson, Y. (2005). Imported giardiasis: impact of international travel, immigration, and adoption. Am J Trop Med Hyg, 72(6), 825-830. Erlandsen, S. L., Bemrick, W. J., & Pawley, J. (1989). High-resolution electron microscopic evidence for the filamentous structure of the cyst wall in Giardia muris and Giardia duodenalis. J Parasitol, 75(5), 787-797. Erlandsen, S. L., Bemrick, W. J., Schupp, D. E., Shields, J. M., Jarroll, E. L., Sauch, J. F., & Pawley, J. B. (1990). High-resolution immunogold localization of Giardia cyst wall antigens using field emission SEM with secondary and backscatter electron imaging. J Histochem Cytochem, 38(5), 625-632. Forterre, P., Gribaldo, S., Gadelle, D., & Serre, M. C. (2007). Origin and evolution of DNA topoisomerases. Biochimie, 89(4), 427-446. Gadelle, D., Filee, J., Buhler, C., & Forterre, P. (2003). Phylogenomics of type II DNA topoisomerases. Bioessays, 25(3), 232-242. Gibson, C., Schanen, B., Chakrabarti, D., & Chakrabarti, R. (2006). Functional characterisation of the regulatory subunit of cyclic AMP-dependent protein kinase A homologue of Giardia lamblia: Differential expression of the regulatory and catalytic subunits during encystation. Int J Parasitol, 36(7), 791-799. Gillin, F. D., Reiner, D. S., Gault, M. J., Douglas, H., Das, S., Wunderlich, A., & Sauch, J. F. (1987). Encystation and expression of cyst antigens by Giardia lamblia in vitro. Science, 235(4792), 1040-1043. Grelon, M., Vezon, D., Gendrot, G., & Pelletier, G. (2001). AtSPO11-1 is necessary for efficient meiotic recombination in plants. EMBO J, 20(3), 589-600. Lin, B. C., Su, L. H., Weng, S. C., Pan, Y. J., Chan, N. L., Li, T. K., . . . Sun, C. H. (2013). DNA topoisomerase II is involved in regulation of cyst wall protein genes and differentiation in Giardia lamblia. PLoS Negl Trop Dis, 7(5), e2218. Hagen, K. D., Hirakawa, M. P., House, S. A., Schwartz, C. L., Pham, J. K., Cipriano, M. J., . . . Dawson, S. C. (2011). Novel structural components of the ventral disc and lateral crest in Giardia intestinalis. PLoS Negl Trop Dis, 5(12), e1442. Hartung, F., & Puchta, H. (2001). Molecular characterization of homologues of both subunits A (SPO11) and B of the archaebacterial topoisomerase 6 in plants. Gene, 271(1), 81-86. Hartung, F., Angelis, K. J., Meister, A., Schubert, I., Melzer, M., & Puchta, H. (2002). An archaebacterial topoisomerase homolog not present in other eukaryotes is indispensable for cell proliferation of plants. Curr Biol, 12(20), 1787-1791. Hetsko, M. L., McCaffery, J. M., Svard, S. G., Meng, T. C., Que, X., & Gillin, F. D. (1998). Cellular and transcriptional changes during excystation of Giardia lamblia in vitro. Exp Parasitol, 88(3), 172-183. Huang, Y. C., Su, L. H., Lee, G. A., Chiu, P. W., Cho, C. C., Wu, J. Y., & Sun, C. H. (2008). Regulation of cyst wall protein promoters by Myb2 in Giardia lamblia. J Biol Chem, 283(45), 31021-31029. Hwang, P. Y., & Hunter, N. (2011). Mapping meiotic breaks: Spo11 oligonucleotides precisely mark the spots. Genome Biol, 12(4), 111. Januschka, M. M., Erlandsen, S. L., Bemrick, W. J., Schupp, D. G., & Feely, D. E. (1988). A comparison of Giardia microti and Spironucleus muris cysts in the vole: an immunocytochemical, light, and electron microscopic study. J Parasitol, 74(3), 452-458. Keeney, S. (2001). Mechanism and control of meiotic recombination initiation. Curr Top Dev Biol, 52, 1-53. Keeney, S. (2008). Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis. Genome Dyn Stab, 2, 81-123. Keeney, S., & Kleckner, N. (1995). Covalent protein-DNA complexes at the 5' strand termini of meiosis-specific double-strand breaks in yeast. Proc Natl Acad Sci U S A, 92(24), 11274-11278. Keister, D. B. (1983). Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg, 77(4), 487-488. Kim, K. T., Mok, M. T., & Edwards, M. R. (2005). Protein kinase B from Giardia intestinalis. Biochem Biophys Res Commun, 334(2), 333-341. Klapholz, S., Waddell, C. S., & Esposito, R. E. (1985). The role of the SPO11 gene in meiotic recombination in yeast. Genetics, 110(2), 187-216. Lauwaet, T., Davids, B. J., Reiner, D. S., & Gillin, F. D. (2007). Encystation of Giardia lamblia: a model for other parasites. Curr Opin Microbiol, 10(6), 554-559. Lauwaet, T., Davids, B. J., Torres-Escobar, A., Birkeland, S. R., Cipriano, M. J., Preheim, S. P., . . . Gillin, F. D. (2007). Protein phosphatase 2A plays a crucial role in Giardia lamblia differentiation. Mol Biochem Parasitol, 152(1), 80-89. Li, S., Fu, Q., Chen, L., Huang, W., & Yu, D. (2011). Arabidopsis thaliana WRKY25, WRKY26, and WRKY33 coordinate induction of plant thermotolerance. Planta, 233(6), 1237-1252. Lujan, H. D., Mowatt, M. R., Byrd, L. G., & Nash, T. E. (1996). Cholesterol starvation induces differentiation of the intestinal parasite Giardia lamblia. Proc Natl Acad Sci U S A, 93(15), 7628-7633. Lujan, H. D., Mowatt, M. R., Conrad, J. T., Bowers, B., & Nash, T. E. (1995). Identification of a novel Giardia lamblia cyst wall protein with leucine-rich repeats. Implications for secretory granule formation and protein assembly into the cyst wall. J Biol Chem, 270(49), 29307-29313. Malewicz, M., & Perlmann, T. (2014). Function of transcription factors at DNA lesions in DNA repair. Exp Cell Res, 329(1), 94-100. Miao, Y., Jiang, J., Ren, Y., & Zhao, Z. (2013). The single-stranded DNA-binding protein WHIRLY1 represses WRKY53 expression and delays leaf senescence in a developmental stage-dependent manner in Arabidopsis. Plant Physiol, 163(2), 746-756. Mori, T., Kurahashi, H., Shinka, T., Nakahori, Y., Taniguchi, M., Toda, T., & Iwamoto, T. (2006). Candidate genes for male factor infertility--validation. Fertil Steril, 86(5), 1553-1554; author reply 1554. Mowatt, M. R., Lujan, H. D., Cotten, D. B., Bowers, B., Yee, J., Nash, T. E., & Stibbs, H. H. (1995). Developmentally regulated expression of a Giardia lamblia cyst wall protein gene. Mol Microbiol, 15(5), 955-963. McKim, K. S., & Hayashi-Hagihara, A. (1998). mei-W68 in Drosophila melanogaster encodes a Spo11 homolog: evidence that the mechanism for initiating meiotic recombination is conserved. Genes Dev, 12(18), 2932-2942. Nichols, M. D., DeAngelis, K., Keck, J. L., & Berger, J. M. (1999). Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11. EMBO J, 18(21), 6177-6188. Paget, T. A., Jarroll, E. L., Manning, P., Lindmark, D. G., & Lloyd, D. (1989). Respiration in the cysts and trophozoites of Giardia muris. J Gen Microbiol, 135(1), 145-154. Pan, Y. J., Cho, C. C., Kao, Y. Y., & Sun, C. H. (2009). A novel WRKY-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia. J Biol Chem, 284(27), 17975-17988. Postow, L., Crisona, N. J., Peter, B. J., Hardy, C. D., & Cozzarelli, N. R. (2001). Topological challenges to DNA replication: conformations at the fork. Proc Natl Acad Sci U S A, 98(15), 8219-8226. Poxleitner, M. K., Carpenter, M. L., Mancuso, J. J., Wang, C. J., Dawson, S. C., & Cande, W. Z. (2008). Evidence for karyogamy and exchange of genetic material in the binucleate intestinal parasite Giardia intestinalis. Science, 319(5869), 1530-1533. Romanienko, P. J., & Camerini-Otero, R. D. (2000). The mouse Spo11 gene is required for meiotic chromosome synapsis. Mol Cell, 6(5), 975-987. Ramesh, M. A., Malik, S. B., & Logsdon, J. M., Jr. (2005). A phylogenomic inventory of meiotic genes; evidence for sex in Giardia and an early eukaryotic origin of meiosis. Curr Biol, 15(2), 185-191. Reiner, D. S., Hetsko, M. L., Meszaros, J. G., Sun, C. H., Morrison, H. G., Brunton, L. L., & Gillin, F. D. (2003). Calcium signaling in excystation of the early diverging eukaryote, Giardia lamblia. J Biol Chem, 278(4), 2533-2540. Reiner, D. S., McCaffery, M., & Gillin, F. D. (1990). Sorting of cyst wall proteins to a regulated secretory pathway during differentiation of the primitive eukaryote, Giardia lamblia. Eur J Cell Biol, 53(1), 142-153. Rizhsky, L., Liang, H., & Mittler, R. (2002). The combined effect of drought stress and heat shock on gene expression in tobacco. Plant Physiol, 130(3), 1143-1151. Robertson, L. J., Hanevik, K., Escobedo, A. A., Morch, K., & Langeland, N. (2010). Giardiasis--why do the symptoms sometimes never stop? Trends Parasitol, 26(2), 75-82. Shingu, Y., Mikawa, T., Onuma, M., Hirayama, T., & Shibata, T. (2010). A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis. FEBS J, 277(10), 2360-2374. Singer, S. M., Yee, J., & Nash, T. E. (1998). Episomal and integrated maintenance of foreign DNA in Giardia lamblia. Mol Biochem Parasitol, 92(1), 59-69. Stacey, N. J., Kuromori, T., Azumi, Y., Roberts, G., Breuer, C., Wada, T., Maxwell, A. Roberts, K.,Sugimoto-Shirasu, K. (2006). Arabidopsis SPO11-2 functions with SPO11-1 in meiotic recombination. Plant J, 48(2), 206-216. Su, L. H., Lee, G. A., Huang, Y. C., Chen, Y. H., & Sun, C. H. (2007). Neomycin and puromycin affect gene expression in Giardia lamblia stable transfection. Mol Biochem Parasitol, 156(2), 124-135. Sugimoto-Shirasu, K., Stacey, N. J., Corsar, J., Roberts, K., & McCann, M. C. (2002). DNA topoisomerase VI is essential for endoreduplication in Arabidopsis. Curr Biol, 12(20), 1782-1786. Sun, C. H., Palm, D., McArthur, A. G., Svard, S. G., & Gillin, F. D. (2002). A novel Myb-related protein involved in transcriptional activation of encystation genes in Giardia lamblia. Mol Microbiol, 46(4), 971-984. Teodorovic, S., Braverman, J. M., & Elmendorf, H. G. (2007). Unusually low levels of genetic variation among Giardia lamblia isolates. Eukaryot Cell, 6(8), 1421-1430. Vos, S. M., Tretter, E. M., Schmidt, B. H., & Berger, J. M. (2011). All tangled up: how cells direct, manage and exploit topoisomerase function. Nat Rev Mol Cell Biol, 12(12), 827-841. Yin, Y., Cheong, H., Friedrichsen, D., Zhao, Y., Hu, J., Mora-Garcia, S., & Chory, J. (2002). A crucial role for the putative Arabidopsis topoisomerase VI in plant growth and development. Proc Natl Acad Sci U S A, 99(15), 10191-10196. Yoder, J. S., Beach, M. J., Centers for Disease, C., & Prevention. (2007). Giardiasis surveillance--United States, 2003-2005. MMWR Surveill Summ, 56(7), 11-18. Yoder, J. S., Harral, C., Beach, M. J., Centers for Disease, C., & Prevention. (2010). Giardiasis surveillance - United States, 2006-2008. MMWR Surveill Summ, 59(6), 15-25. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19646 | - |
| dc.description.abstract | 梨形鞭毛蟲是一種常造成腸道疾病的原蟲類寄生蟲，其主要的症狀有腹瀉、噁心嘔吐、脫水、體重減輕、營養不良等。梨形鞭毛蟲在其生活史上主要有兩種型態：滋養體與囊體，在宿主體內時主要是以滋養體寄生，而當宿主由腸道排出體外後，滋養體會囊體化形成囊體以適應宿主體外惡劣的環境。梨形鞭毛蟲主要行二元分裂，在囊體化過程中會進行核複製，使得染色體套數由4N變為16N，這時可能透過大量表現meiotic gene homologs Spo11, Dmc1a 和 Hop1等，使雙核中的染色體進行類似減數分裂中才會進行的homologous recombination，以交換遺傳物質。
本研究想了解，梨形鞭毛蟲的gSpo11在囊體化過程中所扮演的角色。首先我們利用RT-PCR和Q-PCR來偵測gSpo11在滋養體和囊體化時期的RNA表現量，結果顯示不管是RT-PCR或是Q-PCR，在囊體化時期gSpo11的RNA表現量都明顯增加。接著建構gSpo11-HA重組蛋白質的質體並轉染到梨形鞭毛蟲內，以螢光免疫染色法偵測出gSpo11蛋白質多數分布於梨形鞭毛蟲的細胞核中，少數則分布於細胞質內。而從DNA切割活性實驗中得知gSpo11蛋白質具有切割雙股DNA的能力，並且過程中需要Mg2+離子或Ca2+離子的參與。藉由電泳位移實驗，發現gSpo11蛋白質具有結合雙股DNA與單股DNA的能力。然後由Q-PCR、西方點墨法與囊體計數得知，大量表現gSpo11蛋白質會增加囊體化相關基因cwp1-3、myb2、wrky以及topoII基因的表現與囊體形成的能力。然而當我們將gSpo11蛋白質在Toprim domain上兩個高保留性的胺基酸進行突變後，可發現gSpo11突變蛋白質其多數分布位置由梨形鞭毛蟲的細胞核改為細胞質，並且會使降低囊體化相關基因的表現與囊體形成的能力。而由染色質免疫沉澱的結果可得知gSpo11蛋白質能與囊體壁蛋白質CWP1-3的基因啟動子結合，並且由免疫沉澱法的結果顯示，gSpo11蛋白質具有與調控梨形鞭毛蟲囊體化相關轉錄因子WRKY和MYB2結合的現象。 我們的研究結果顯示，梨形鞭毛蟲的gSpo11會在囊體化時期大量表現，並且能活化囊體化相關基因的表現，我們認為這可能與gSpo11蛋白質能與囊體壁蛋白質的基因啟動子結合，並能與調控囊體化相關轉錄因子產生交互作用有關。 | zh_TW |
| dc.description.abstract | Giardia lamblia is a kind of intestinal protozoan parasite which can cause disease called giardiasis. Symptoms include diarrhea, dehydration, nausea, vomiting and malnutrition. The life cycle of G. lamblia has two stages: a binucleate trophozoite and a quadrinucleate infectious cyst. G. lamblia divides through asexual, binary fission to reproduce. Evidence shows that Giardia has low levels of allelic heterozygosity in two nuclei. Some homologs of meiosis genes (HMGs) have been found in Giardia, including Spo11, Dmc1, Hop1, which only express in the cyst. It might indicate that the genetic materials of two nuclei exchange in encystation.
To identify the role of Spo11 protein in G. lamblia encystation, we used quantitative RT-PCR and Western blots to detect the gSpo11 RNA and proteins in G. lamblia. Result shows that gSpo11 gene was up-regulated expression during the encystation stage. Immunofluorescence assay revealed that gSpo11 was major localized to nucleus. DNA cleavage assay indicated that gSpo11 can cleavage dsDNA, with the cofactors Mg2+ or Ca2+. By electrophoretic mobility shift assay, we showed that gSpo11 is able to bind ssDNA and dsDNA. Also we found the overexpression of gSpo11 can induce the expression of cwp1-3、myb2、wrky and topoII genes, and cyst formation. But when we introduced G202A and R209A mutations in the toprim domain of gSpo11, we found that mutant protein changed localization to cytoplasm, also the function of induction the expression of genes and cyst formation was inhibited comparing with wild-type protein. Use chromatin immunoprecipitation, we found that gSpo11 can bind the promotors of cwp1-3 gene. By co-immunoprecipitation assay, it indicated that gSpo11 can interact with encystation relating transcription factors, Myb2 and WRKY. To sum up, this study suggests that gSpo11 plays an important role in induction of the cwp genes and encystation relating transcription factors. It might be related to gSpo11 can interact with Myb2 and WRKY, and bind the promotors of cwp genes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:11:10Z (GMT). No. of bitstreams: 1 ntu-105-R02445201-1.pdf: 2607423 bytes, checksum: 655fe802608158f08b538fa30dd7e6ac (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 誌謝………………………………………………...……………………………...….. i
摘要…………………………………………………..……………….…............…… ii Abstract…………………………………………………………..………………..…. iv 目錄…………………………………………………………..……………............... vi 第一章 前言……………………………………..…………………………….. 1 1.1 梨形鞭毛蟲簡介………………………………………..…..…………….... 1 1.2 DNA拓樸異構酶…………………………………….…………………….. 2 1.3 Topoisomerase VI A subunit…………………………….…………………... 3 1.4 研究動機…………………………………….……………………………... 5 第二章 材料與方法..………………………………………………………….. 6 2.1 梨形鞭毛蟲細胞株的培養 (G. lamblia Culture)………………………… 6 2.2轉殖質體的建構 (Plasmid Construction)………………………………… 6 2.2.1 5’ 5N-pac…..…………………………………………………………… 6 2.2.2 pPgSpo11…………..…………………………………………………… 6 2.2.3 pPgSpo11m5…….....…………………………………………………… 7 2.3 梨形鞭毛蟲的轉染與選殖 (Stable DNA Transformation and Selection)… 7 2.4 免疫螢光染色 (Immunofluorescence Assay)…………………………… 8 2.5 西方點墨法與 (Western Blot Analysis) 與Coomassie Blue染色……… 8 2.5.1 TCA蛋白質沉澱 (TCA protein precipitation)………………………… 8 2.5.2 西方點墨法 (Western Blot Analysis)………………………………… 9 2.5.3 Coomassie Blue染色…..……………………………………………… 9 2.6 重組蛋白質的表現與純化 (Expression and Purification of Recombinatant gSpo11 Protein)………………………………………..………………………… 10 2.6.1 重組蛋白質gSPO11以及突變gSPO11蛋白質的表現載體建構…... 10 2.6.2 重組蛋白質的表現與純化…………………….…………………….. 10 2.7 負超螺旋切割DNA活性分析 (DNA Cleavage Assay)………………… 11 2.8 電泳位移實驗 (Electrophoretic Mobility Shift Assay，EMSA) ..………. 11 2.9 反轉錄聚合酶鏈式反應 (Semi-quantitative RT-PCR Analysis，RT-PCR).. 11 2.10 即時定量反轉錄聚合酶鏈式反應 (Quantitative Real-time PCR Analysis，Q-PCR )……………………………………………………………….......…… 13 2.11 囊體計數 (Cyst Count)………..……………………………………… 15 2.12 免疫共沉澱法 (Co-immunoprecipitation Assay)……………………… 15 2.13 染色質免疫沉澱 (Chromatin Immunoprecipitation，ChIP)…………… 15 第三章 實驗結果………………………………….………………….……... 18 3.1 分析梨形鞭毛蟲topoisomerase VI A (gSpo11) 序列…………………… 18 3.2 梨形鞭毛蟲的gSpo11基因在囊體化時期表現量會上升…………….… 19 3.3 梨形鞭毛蟲的gSpo11蛋白質具有對DNA的切割能力…………….… 19 3.4 梨形鞭毛蟲的gSpo11蛋白質具有結合雙股DNA與單股DNA的能力.. 20 3.5 利用突變蛋白質來分析梨形鞭毛蟲的gSpo11蛋白質的活性區域….… 20 3.6 gSpo11蛋白質能與cwp1、cwp2和cwp3的基因啟動子結合………….… 22 3.7 梨形鞭毛蟲的gSpo11蛋白質可誘導TOPOII和部分囊體化相關轉錄因子的蛋白質表現量增加…………………………………………………………. 23 3.8 gSpo11蛋白質能與囊體化相關轉綠因子WRKY和MYB2交互作用.… 23 第四章 討論….………………………….…….…………….……….…….…... 25 4.1 梨形鞭毛蟲gSpo11蛋白質功能區域分析………………….……….… 25 4.2 梨形鞭毛蟲gSpo11蛋白質能誘導TOPOII以及部分囊體化相關轉錄因子的表現量上升……………………………………………………………….… 25 4.3 梨形鞭毛蟲gSpo11蛋白質與囊體化相關轉錄因子WRKY的交互作用 26 4.4 分析gSpo11蛋白質能與cwp1、cwp2和cwp3的基因啟動子結合…....… 27 附圖………………..…………………………………………………..………….… 28 附表…………………………………………………………………..………….… 53 參考文獻……………………………………………………………..………….… 54 | |
| dc.language.iso | zh-TW | |
| dc.title | 探討Spo11在梨形鞭毛蟲囊體化過程中所扮演的角色 | zh_TW |
| dc.title | Identification of a role of Spo11 Protein in
Giardia lamblia Encystation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 詹迺立,李財坤 | |
| dc.subject.keyword | 梨形鞭毛蟲,囊體化,type IIB topoisomerase,topoisomerase VI A subunit,gSpo11,double-strand breaks (DSB), | zh_TW |
| dc.subject.keyword | Giardia lamblia,Encystation,type IIB topoisomerase,topoisomerase VI A subunit,gSpo11,double-strand breaks (DSB), | en |
| dc.relation.page | 59 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2016-01-26 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-105-1.pdf 未授權公開取用 | 2.55 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。