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標題: | 以固相微萃取發展雙酚A及雙酚A擬似物之分析方法 Determinations of Bisphenol A and Bisphenol A Analogues by Solid-Phase Microextraction with On-Fiber Derivatizations |
作者: | Fu-Chiung Hsu 許芙瓊 |
指導教授: | 蔡詩偉 |
關鍵字: | 雙酚 A,雙酚 A 擬似物,固相微萃取,氣相層析質譜儀, BPA,BPA analogues,SPME,On-fiber derivatization,GC/MS, |
出版年 : | 2016 |
學位: | 碩士 |
摘要: | 雙酚 A (bisphenol A; BPA)常出現在電子產品和塑膠製品中,包括:聚碳酸脂塑膠、罐頭內層塗料和電器電腦用品等。雙酚 A 廣泛流布在空氣、水體和土壤環境中,並主要經由攝食進入人體。目前已知雙酚 A 是一種內分泌干擾物質,其會擾亂人體內分泌系統,破壞荷爾蒙平衡,造成不孕和生殖異常的機率增加,且會增加與荷爾蒙有關之癌症(如乳癌、睪丸癌及前列腺癌)發生率。
各國法規逐漸禁止雙酚 A 之使用,因此使用雙酚 A 的業者開始選擇結構相似之雙酚 A 擬似物(如雙酚 S、雙酚 B、雙酚 E、雙酚 F 等)代替;然而這些取代物亦有研究發現具有跟雙酚 A 相似之毒性,但過去對於同時分析多種雙酚 A 擬似物之方法較少,且分析檢測雙酚 A 與其擬似物之需求日漸增高,因此開發一穩定之分析方法可同時測量多種雙酚類具有其必性。 本研究利用固相微萃取(Solid-phase microextraction, SPME)技術搭配氣相層析質譜儀進行相關分析,並著重於開發新的分析方法;此方法可以一次檢測九種雙酚類物質(包含: 雙酚 A、雙酚 B、雙酚 E、雙酚 F、雙酚 S、雙酚AP、雙酚 AF、雙酚 AP、雙酚 P),而固相微萃取之優點為較過去之方法更為快速且無使用任何有機溶劑,較為環保。 此研究驗證最適之萃取條件為在攝氏 50 度之環境下萃取 20 分鐘,衍生條件則是添加 10 微升之衍生試劑 BSTFA 在攝氏 60 度的環境下衍生一分鐘;衍生後將固相微萃取裝置置於氣相層析儀在攝氏 280 度下脫附 4 分鐘,此脫附效率為 100%,隨後以氣相層析儀分析九種化合物,包含雙酚 A 及雙酚 A 擬似物,此分析方法的線性範圍分別為 0.1 μg/L ~ 10 μg/L。 雙酚 A 議題已被討論多年,近年來其慢性暴露所造成之健康影響更是受到重視;而雙酚 A 及其擬似物需要更深入的研究,無論是對環境的影響或是健康危害方面,建立更完整的風險評估模式是被需要的。因此,本研究所完成雙酚 A 及其擬似物之方析方法,將可提供一個更快速且環境友善之方法,並應用於環境流佈調查、暴露評估等方面,以提供更完整之資訊。 Bisphenol A (BPA) is widely used in consumer products, such as the lining in various types of cans. The leaching of BPA from the products can result in human exposures. For example, it was indicated that BPA could be released into canned foods and cause possible adverse effects, especially for infants. Many countries have banned the use of BPA in food packaging. Hence, various substitutes of BPA, including bisphenol S (BPS), bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPAP), bisphenol P (BPP), bisphenol AP (BPAP), bisphenol AF (BPAF), and bisphenol BP (BPBP), become available in the market. However, previous studies also showed that exposing to hose substitutes might still cause same or more serious health concerns. Therefore, to assess the health risk from possible sources of exposures, there is a need to determine the concentrations of BPA and BPA analogues. In this study, BPA and BPA analogues were first extracted by using solidphase microextraction (SPME) with direct sample immersions. The analytes adsorbed on the SPME fiber were then derivatized with N,O-Bis(trimethylsilyl) trifluoroacetamide (BSTFA). Afterwards, the derivatives were determined with gas chromatograph and mass pectrometry (GC/MS). The method developed in this research was performed to determine the concentrations of BPA and BPA analogues in infant formula, which were purchased from the markets. The samples were mixed with water before centrifuged and the SPME procedures. The results showed that the desorption efficiency was 100% when the desorption time was 4 min under 280℃. The best suitable fiber coating was 85μm Polyacrylate (PA) fiber. The optimum condition of extraction was 20 minutes at 50℃ and derivatization was 1 minutes at 60 ℃ by adding 10 μL of BSTFA. The working range for the analysis was between 0.1 – 10 μg/L. Different levels of BPA and BPA analogues were found in commercially available infant formula. Compared with conventional extraction methods, the SPME provides a timesaving and solve-free procedure. In addition, the sensitivities of the method for BPA and BPA analogues were low enough to determine the concentrations from various types of samples. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19078 |
DOI: | 10.6342/NTU201602774 |
全文授權: | 未授權 |
顯示於系所單位: | 環境衛生研究所 |
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