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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蕭信宏(Shin-Hong Shiao) | |
| dc.contributor.author | Ya-Yin Cheng | en |
| dc.contributor.author | 鄭雅尹 | zh_TW |
| dc.date.accessioned | 2021-06-08T01:12:38Z | - |
| dc.date.copyright | 2014-10-09 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-14 | |
| dc.identifier.citation | Ahmed, A. M., Maingon, R., Romans, P., & Hurd, H. (2001). Effects of malaria infection on vitellogenesis in Anopheles gambiae during two gonotrophic cycles. Insect Mol Biol, 10(4), 347-356.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18575 | - |
| dc.description.abstract | 蚊子在許多傳染疾病當中扮演著相當重要的角色，主要是瘧疾、登革熱、黃熱病、西尼羅熱、絲蟲病等疾病傳播的媒介。其中，登革熱是一種不可輕忽而且具有威脅性的傳染病，根據世界衛生組織的統計，全世界至少有二十五億的人口是暴露在感染登革熱的風險當中，並且估計全球每年大約有5000萬個登革熱病例；而臺灣在2013年就有將近1000個登革熱病例。由於至今尚未有登革疫苗問世，再加上仍無有效對抗登革熱的藥物，所以目前為止對於登革熱只能採取支持性的療法；因此，藉由控制病媒蚊的數量來抑制蟲媒疾病傳播，並且發展新的病媒控制方法來防治登革熱，是現今重要的研究主題。本研究以埃及斑蚊做為研究模式，來探討病媒蚊在感染登革病毒時，其免疫系統與生殖調控之相關機制，希望藉由釐清其詳細調控機制，找出控制登革病毒傳播的可能性。從初步的實驗結果當中，我們發現埃及斑蚊感染登革病毒之後，埃及斑蚊之母蚊產卵數量有顯著下降的趨勢。為了深入瞭解埃及斑蚊感染登革病毒後對生殖調控的影響與其相關機制，我們首先想釐清登革病毒感染與埃及斑蚊卵黃生成的關係。初步結果發現當埃及斑蚊受到登革病毒感染之後，Vitellogenin (Vg) 的表現量無論是在轉錄還是轉譯的階段皆明顯被抑制；此外，我們更進一步發現母蚊之脂肪體中TOR訊息傳遞路徑下游之S6K磷酸化反應受到抑制。因此，我們認為登革病毒極有可能藉由影響TOR訊息傳遞路徑，以達到抑制埃及斑蚊產卵能力的效果。本研究結果將有助於未來應用在病媒傳病能力之研究及病媒控制策略之制訂。 | zh_TW |
| dc.description.abstract | The female mosquito Aedes aegypti plays a pivotal role in bloodmeal acquisition and reproduction, and thereby dengue virus (DENV) transmission. Dengue fever is one of the most devastating arthropod-borne diseases. The WHO reported some 2.5 billion people were at risk from dengue and estimated that there may be 50 million cases of dengue infection worldwide every year. Almost 1000 cases of dengue infection were reported in Taiwan at 2013. Up to now, no effective dengue vaccine or drug has been developed. Therefore, any possible avenue for developing novel control strategies against mosquito-borne diseases is urgently needed. In our preliminary results, we showed a significant reduction of the egg production was exhibited in the mosquitoes infected with dengue virus. To elucidate the impact and underlying mechanisms of DENV infection on mosquito reproduction, we first examined the effects of vitellogenesis upon DENV infection. The expression of vitellogenin was significantly inhibited in terms of transcription and translation level when female mosquitoes were fed by infectious blood meal. Components of a nutrient-sensitive signaling pathway, the TOR signaling pathway, were examined in the dengue virus infected mosquitoes. We showed a significant inhibition of the phosphorylation of S6K, a downstream target of TOR signaling, in the mosquito fat body when taking an infectious blood meal. Our results indicated that DENV infection in the mosquito leads to the inhibition of egg production. This particular effect is regulated by TOR signaling pathway. Information gathered in this study will pave the way toward the establishment of efficient strategies for vector control by using molecular engineering approaches. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T01:12:38Z (GMT). No. of bitstreams: 1 ntu-103-R01445203-1.pdf: 13994651 bytes, checksum: ef3a1d7e3d8f0fa139821b7c9d9fb0c7 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 i 誌謝 ii 中文摘要 iv Abstract v 目錄 vi 圖目錄 ix 表目錄 xi 第一章 緒論 1 1.1. 病媒蚊傳播疾病 (Mosquito-borne disease) 1 1.1.1. 瘧疾 (Malaria) 3 1.1.2. 登革熱 (Dengue fever) 4 1.1.3. 台灣地區登革熱流行病學 5 1.2. 埃及斑蚊之生活史 (Mosquito life cycle) 6 1.3. 埃及斑蚊卵黃生成機制 (Vitellogenesis) 7 1.4. TOR訊息傳遞路徑介紹 (TOR signaling pathway) 8 1.5. 感染病原體對於病媒蚊之影響 10 1.6. 實驗動機與假說 11 第二章 實驗材料與方法 12 2.1. 實驗步驟與流程 12 2.1.1. 埃及斑蚊之飼養與繼代 12 2.1.2. 埃及斑蚊之餵血 13 2.1.3. 病毒注射 (Virus injection) 13 2.1.4. 添加胺基酸餵血實驗 13 2.1.5. 20-hydroxyecdysone (20E) 混合糖水餵食實驗 14 2.1.6. 產卵實驗 (Egg production assay) 14 2.1.7. 孵化率實驗 (Hatching assay) 15 2.1.8. 卵泡 (Follicle) 大小之量化與計數 15 2.1.9. 埃及斑蚊RNA萃取 (RNA extraction) 15 2.1.10. 病毒RNA萃取 (RNA extraction) 16 2.1.11. 反轉錄作用 (Reverse transcription, RT) 16 2.1.12. 聚合酶連鎖反應 (Polymerase chain reaction, PCR) 17 2.1.13. 即時定量聚合酶連鎖反應 (Real-time PCR, Quantitative PCR) 18 2.1.14. 質體建構 (Plasmid construction) 18 2.1.15. 西方點墨法 (Western blot) 19 2.1.16. 細胞培養 (Cell culture) 20 2.1.17. 病毒培養 (Virus culture) 21 2.1.18. 溶斑試驗 (Plaque assay) 21 2.2. 實驗試劑之製備 22 第三章 結果 26 3.1. 埃及斑蚊感染登革病毒之RNA表現情形 26 3.2. 埃及斑蚊體內登革病毒之Plaque assay 26 3.3. 埃及斑蚊感染登革病毒對於母蚊產卵與蚊卵孵化之影響 27 3.4. 埃及斑蚊感染登革病毒對於母蚊Ovary與Follicle之影響 28 3.5. 注射病毒於埃及斑蚊內之RNA表現情形 29 3.6. 注射病毒於埃及斑蚊內對於母蚊產卵之影響 30 3.7. 注射病毒於埃及斑蚊內對於母蚊Ovary與Follicle之影響 30 3.8. 埃及斑蚊感染登革病毒對於母蚊二次產卵之影響 32 3.9. 埃及斑蚊感染登革病毒之Vg表現情形 32 3.10. 埃及斑蚊感染登革病毒之其他YPPs RNA表現情形 33 3.11. 埃及斑蚊感染登革病毒之P-S6K表現情形 34 3.12. 埃及斑蚊感染登革病毒之TOR pathway其他相關基因RNA表現情形 34 3.13. 埃及斑蚊感染登革病毒之Amino acid transporters RNA表現情形 35 3.14. 胺基酸對於埃及斑蚊感染登革病毒後母蚊產卵之影響 36 3.15. 胺基酸對於TOR訊息傳遞路徑與其下游之影響 37 3.16. 登革病毒之各胺基酸含量與其對埃及斑蚊感染登革病毒後產卵之影響 38 3.17. 20E對於埃及斑蚊感染登革病毒之影響 39 3.18. EcR-A對於埃及斑蚊感染登革病毒之影響 39 第四章 討論 41 附圖 50 附表 87 參考文獻 92 圖目錄 圖一 埃及斑蚊卵黃生成機制 50 圖二 TOR訊息傳遞路徑 51 圖三 埃及斑蚊感染登革病毒之RNA表現情形 53 圖四 埃及斑蚊體內登革病毒之Plaque assay 55 圖五 埃及斑蚊感染登革病毒對於母蚊產卵之影響 56 圖六 埃及斑蚊感染登革病毒對於蚊卵孵化之影響 57 圖七 埃及斑蚊感染登革病毒對於母蚊卵巢 (Ovary) 之影響 58 圖八 埃及斑蚊感染登革病毒對於母蚊卵泡 (Follicle) 之影響 60 圖九 注射病毒於埃及斑蚊內之RNA表現情形 61 圖十 注射病毒於埃及斑蚊內對於母蚊產卵之影響 62 圖十一 注射病毒於埃及斑蚊內對於母蚊卵巢 (Ovary) 之影響 63 圖十二 注射病毒於埃及斑蚊內對於母蚊卵泡 (Follicle) 之影響 65 圖十三 埃及斑蚊感染登革病毒對於母蚊二次產卵之影響 66 圖十四 埃及斑蚊感染登革病毒之Vg表現情形 67 圖十五 埃及斑蚊感染登革病毒之其他YPPs RNA表現情形 68 圖十六 埃及斑蚊感染登革病毒之P-S6K表現情形 69 圖十七 埃及斑蚊感染登革病毒之TOR pathway其他相關基因RNA表現情形 72 圖十八 埃及斑蚊感染登革病毒之CATs RNA表現情形 75 圖十九 埃及斑蚊感染登革病毒之HATs RNA表現情形 78 圖二十 胺基酸對於埃及斑蚊感染登革病毒後母蚊產卵之影響 79 圖二十一 胺基酸對於TOR訊息傳遞路徑與其下游之影響 80 圖二十二 組成登革病毒之各胺基酸含量 82 圖二十三 個別胺基酸對於埃及斑蚊感染登革病毒後產卵之影響 84 圖二十四 20E對於埃及斑蚊感染登革病毒之影響 85 圖二十五 EcR-A對於埃及斑蚊感染登革病毒之影響 86 表目錄 表一 實驗所使用之PCR引子序列 87 表二 西方點墨法所用之抗體 90 表三 西方點墨法所用之抗體濃度與操作條件 91 | |
| dc.language.iso | zh-TW | |
| dc.title | 埃及斑蚊感染登革病毒對其生殖調控之影響 | zh_TW |
| dc.title | Effect of dengue virus infection in the regulation of reproduction in the mosquito Aedes aegypti | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 伍安怡(Betty Wu-Hsieh),陳俊宏(Chun-Hong Chen) | |
| dc.subject.keyword | 埃及斑蚊,登革病毒,TOR 訊息傳遞路徑,卵黃生成作用,生殖, | zh_TW |
| dc.subject.keyword | Aedes aegypti,dengue virus,TOR signaling,vitellogenesis,reproduction, | en |
| dc.relation.page | 98 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2014-08-15 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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