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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 余明俊(Ming-Jiun Yu) | |
dc.contributor.author | Pei-Yu Chen | en |
dc.contributor.author | 陳珮毓 | zh_TW |
dc.date.accessioned | 2021-06-08T01:07:19Z | - |
dc.date.copyright | 2014-10-09 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-19 | |
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(2004) Delivery of raft-associated, GPI-anchored proteins to the apical surface of polarized MDCK cells by a transcytotic pathway. Nat Cell Biol 6: 297-307. 23. Newman TM, Tian M & Gomperts BD. (1996) Ultrastructural characterization of tannic acid-arrested degranulation of permeabilized guinea pig eosinophils stimulated with GTP-gamma-S. Eur J Cell Biol 70(3): 209-220. 24. Nielsen S, Terris J, Smith CP, Hediger MA, Ecelbarger CA & Knepper MA. (1996) Cellular and subcellular localization of the vasopressin-regulated urea transporter in rat kidney. Proc Natl Acad Sci USA 93(11): 5495-5500. 25. Nielsen S, DiGiovanni SR, Christensen EI, Knepper MA & Harris HW. (1993) Cellular and subcellular immunolocalization of vasopressin-regulated water channel in rat kidney. Proc Natl Acad Sci USA 90(24): 11663-11667. 26. Perez P, Puertollano R & Alonso MA. (1997) Structural and biochemical similarities reveal a family of proteins related to the MAL proteolipid, a component of detergent-insoluble membrane microdomains. Biochem Biophys Res Commun 232(3): 618-621. 27. Madrid R, Aranda JF, Rodriguez-Fraticelli AE, Ventimiglia L, Andres-Delgado L, Shehata M, Fanayan S, Shahheydari H, Gomez S, Jimenez A, Martin-Belmonte F, Byrne JA & Alonso MA. (2010) The formin INF2 regulates basolateral-to-apical transcytosis and lumen formation in association with Cdc42 and MAL2. Dev Cell 18(5): 814-827. 28. Yu MJ, Pisitkun T, Wang G, Aranda JF, Gonzales PA, Tchapyjnikov D, Shen RF, Alonso MA & Knepper MA. (2008) Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct. Am J Physiol Cell Physiol 295(3): C661-C678 29. Sanchez-Pulido L, Martin-Belmonte F, Valencia A & Alonso MA. (2002) MARVEL: a conserved domain involved in membrane apposition events. Trends Biochem Sci 27(12): 599-601. 30. Marazuela M & Alonso MA. (2004) Expression of MAL and MAL2, two elements of the protein machinery for raft-mediated transport, in normal and neoplastic human tissue. Histol Histopathol 19(3): 925-933. 31. Kamsteeg EJ, Duffield AS, Konings IB, Spencer J, Pagel P, Deen PM & Caplan MJ. (2007) MAL decreases the internalization of the aquaporin-2 water channel. Proc Natl Acad Sci USA 104(42): 16696-16701. 32. Jakob V, Schreiner A, Tikkanen R & Starzinski-Powitz A. (2006) Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs. Mol Biol Cell 17(8): 3397-3408. 33. Paladino S, Pocard T, Catino MA & Zurzolo C. (2006) GPI-anchored proteins are directly targeted to the apical surface in fully polarized MDCK cells. J Cell Biol 172(7): 1023-1034. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18479 | - |
dc.description.abstract | 當腎臟集尿管細胞受到抗利尿激素(antidiuretic hormone vasopressin)的刺激後,會促使細胞中的第二型水通道蛋白(aquaporin-2, AQP2)由細胞內液胞被運送到細胞頂膜(apical plasma membrane),藉此調節腎臟集尿管對水分的排除。在先前的蛋白質體學研究中鑑定到Mal2,一個已知與細胞頂膜蛋白質之胞轉作用(transcytosis)有關的脂筏(lipid raft)蛋白質,因此在這個研究中,我們想探討Mal2是否會參與並調解受抗利尿激素刺激後,AQP2運送至細胞頂膜的胞轉作用。在抗利尿激素類似物(dDAVP)刺激小鼠集尿管細胞株(mpkCCD)後的不同時間點,以免疫螢光染色(immunofluorescence staining)的方法觀察到,在短時間dDAVP的處理過後,AQP2會出現在細胞的側膜。而隨著dDAVP處理時間的增加,位於細胞側膜的AQP2逐漸減少,伴隨著在細胞頂膜的AQP2逐漸增加,這意味著當細胞受到dDAVP的刺激後,AQP2被運送到頂膜的過程會透過胞轉機制。當mpkCCD細胞的側膜被單寧酸(tannic acid)固定,再經dDAVP或forskolin刺激後,可以觀察到一部分的AQP2聚集在細胞的側邊區域,而有另一部分的AQP2則是聚集在細胞頂膜,表示當細胞受到dDAVP或forskolin刺激後,AQP2從細胞內液胞被送至頂膜的路徑分為兩條:其一為直接運送,其二則透過胞轉機制。然而在不表現Mal2的mpkCCD 細胞中,當此細胞的側膜被單寧酸(tannic acid)固定後,此時forskolin的刺激無法誘導AQP2聚集在細胞的側邊區域,此結果說明了Mal2會參與並調解集尿管細胞受抗利尿激素刺激後,AQP2運送至細胞頂膜的胞轉作用。 | zh_TW |
dc.description.abstract | In kidney collecting duct cells, vasopressin (AVP) triggers redistribution of aquaporin-2 (AQP2) water channel protein from intracellular vesicles to the apical plasma membrane to regulate renal water excretion. In our previous proteomic study, we identified Mal2, a lipid-raft membrane protein known to mediate apical membrane protein trafficking via transcytosis. This study examined roles of Mal2 in AVP-induced apical AQP2 trafficking via transcytosis. Time course immunofluorescence showed staining of AQP2 in the lateral plasma membrane of the mpkCCD cells after short-term AVP analog dDAVP stimulation. The lateral AQP2 staining disappeared gradually with concomitant appearance of apical AQP2 staining, suggesting indirect transcytotic mechanism for apical AQP2 targeting upon dDAVP stimulation. When the lateral membrane was fixed with tannic acid, a portion of AQP2 accumulated in the lateral space of the cells in response to dDAVP or forskolin. Note that another portion of AQP2 was detected in the apical space of the cells. The observations indicated that dDAVP or forskolin induced apical AQP2 targeting via both direct and indirect transcytotic pathway. The forskolin-induced lateral AQP2 staining in the presence of tannic acid was completely absent in the cells lacking Mal2. Our data are compatible with a role of Mal2 in vasopressin-induced indirect apical AQP2 targeting via the transcytotic mechanism. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T01:07:19Z (GMT). No. of bitstreams: 1 ntu-103-R01442011-1.pdf: 1376392 bytes, checksum: e593c7232300d71afa36d73ba15fe3bd (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 誌謝 I
中文摘要 II Abstract III 1. Introduction 1 2. Materials 7 3. Methods 13 4. Results 21 Vasopressin induced indirect apical AQP2 targeting 22 Vasopressin induced lateral AQP2 accumulation of AQP2 when the lateral membrane fusion was blocked with tannic acid 24 A Mal2-depleted mpkCCD cell colony was cloned 26 In the cells lacking Mal2, forskolin-induced lateral AQP2 staining in the presence of tannic acid was completely absent 27 5. Figures and Legends 28 6. Discussion 38 7. References 44 | |
dc.language.iso | en | |
dc.title | 抗利尿激素誘導第二型水通道蛋白透過Mal2調節的胞轉機制運送到腎臟集尿管細胞的頂膜 | zh_TW |
dc.title | Vasopressin Induces Apical Aquaporin-2 Trafficking via Mal2-Mediated Transcytosis in Renal Collecting Duct Cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李芳仁(Fang-Jen Lee),林水龍(Shuei-Liong Lin),龔秀妮(Hsiu-Ni Kung) | |
dc.subject.keyword | 抗利尿激素,第二型水通道蛋白,腎臟集尿管細胞,蛋白Mal2, | zh_TW |
dc.subject.keyword | Vasopressin,Aquaporin-2,Renal collecting duct cells,Mal2, | en |
dc.relation.page | 47 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2014-08-19 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 生物化學暨分子生物學研究所 | zh_TW |
顯示於系所單位: | 生物化學暨分子生物學科研究所 |
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