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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王培育(Pei-Yu Wang) | |
| dc.contributor.author | Hao-Chih Hung | en |
| dc.contributor.author | 洪浩植 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:44:57Z | - |
| dc.date.copyright | 2015-09-25 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-05 | |
| dc.identifier.citation | Abbott, F. V., Franklin, K. B., Westbrook, R. F. (1995). The formalin test: scoring properties of the first and second phases of the pain response in rats. Pain, 60(1), 91-102.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17862 | - |
| dc.description.abstract | 在先前的研究中指出，透過血清素濃度的調節，能夠影響與傷害性感覺相關的行為及生理功能，然而血清素是如何調控癢覺訊號的傳遞至今仍尚未清楚。血清素的受體可分為許多種亞型，其中血清素受體亞型6型(HTR6)表現在背根神經節與脊椎背角，而此處與癢覺訊息傳遞有很大的關聯性。因此，在本研究之中，便是想探討HTR6是否參與了癢覺傳遞調控。為了理解HTR6是否參與了組織胺引發的癢覺訊號傳遞路徑，我們使用了搔抓行為實驗來檢測其癢覺程度在剔除HTR6 基因小鼠是否有所差異。結果表明，剃除HTR6基因的小鼠比起正常的小鼠，在皮下注射組織胺後有較為明顯的搔抓行為。這代表HTR6信號可能參與了抑制了組織胺引發的癢覺訊號傳導。除此之外，我們也檢測了HTR6是否也參與非組織胺引發的癢覺傳導路徑，在此實驗中，我們使用了氯奎寧做為非組織胺性的致癢源。然而結果顯示，HTR6可能僅作用於組織胺引發的癢覺傳遞路徑，因為剃除HTR6基因小鼠與正常小鼠，在皮下注射氯奎寧後搔抓行為實驗中並沒有顯著差異。 | zh_TW |
| dc.description.abstract | It has been shown in previous studies that serotonin could modulate behavioral and physiological functions of nociception by ascending or descending pathway, but how it regulates itch signal transduction is still unclear. There are several subfamilies of serotonin receptors and serotonin 6 receptor (HTR6) is expressed in dorsal root ganglion and dorsal spinal cord, which may involve in itch responses. Here I will investigate whether HTR6 is involved in the regulation of itch responses. To quantify my findings, I will evaluate the level of itch response in HTR knock-out by scratching behavior test. In this study, I found that the number of scratching bouts of HTR6 knock-out mice was significantly higher than wild-type mice after an intradermal injection of histamine. The results suggested that HTR6 signaling may play a role in inhibition of itch signal transduction. Moreover, HTR6-mediated itch response was likely to be a histamine-dependent effect since intradermal injection of chloroquine did not elicit alternation of scratching behavior between two genotypes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:44:57Z (GMT). No. of bitstreams: 1 ntu-104-R02454002-1.pdf: 1837721 bytes, checksum: 472819a394797ac90d391b6ba63da532 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | Content
Acknowledgment -Ⅰ 中文摘要 - Ⅱ Abstract - Ⅲ Content - Ⅳ Chapter 1. Introduction. - p.1 Chapter 2. Material and method - p.8 Ⅰ. Animals - p.8 Ⅱ. Molecular tests - p.9 Ⅱ-1. Tissue dissection - p.9 Ⅱ-2. RNA extractionp - .9 Ⅱ-3. Quantitative Real-Time PCR - p.10 Ⅱ-4. Data analysis - p.11 Ⅲ. Behavior tests - p.12 Ⅲ-1. Drugs and reagents - p.12 Ⅲ-2. Scratching behavior test - p.12 Ⅲ-3. Data analysis - p.13 Chapter 3.Results - p.14 Ⅰ.Molecular tests - p.14 Ⅰ-1. HTR6 mRNA was expressed in spinal cord - p.14 Ⅰ-2. HTR6 mRNA was expressed in HTR6 WT mice spinal cord but not HTR6 KO mice spinal cord - p.15 Ⅱ. Scratching behavior tests - p.16 Ⅱ-1. Scratching behavior induced by different concentrations of histamine - p.16 Ⅱ-2. Histamine at higher concentration enhances scratching behavior in HTR6 KO mice - p.17 Ⅱ-3. Histamine at lower concentration causes normal scratching behavior in HTR6 KO mice - p.18 Ⅱ-4. Histamine at higher concentration causes normal scratching behavior in mice injected with HTR6 antagonist - p.19 Ⅱ-5. CQ induces normal scratching behavior in HTR6 KO mice - p.20 Chapter 4.Discussion - p.22 Chapter 5.Figures - p.27 Fig.1. HTR6 mRNA is expressed in spinal cord and DRG - p.27 Fig.2. HTR6 mRNA expression level in spinal cord in WT and HTR6 KO mice - p.28 Fig.3. Scratching behavior induced by different concentrations of histamine - p.29 Fig.4. Histamine at higher concentration enhances scratching behavior in HTR6 KO mice - p.30 Fig.5. Histamine at lower concentration causes normal scratching behavior in HTR6 KO mice - p.32 Fig.6. Histamine at higher concentration causes normal scratching behavior in mice injected with HTR6 antagonist - p.34 Fig.7. Histamine at higher concentration causes normal scratching behavior in mice injected with HTR6 agonist - p.36 Fig.8. CQ induces normal scratching behavior in HTR6 KO mice………….…p.38 Chapter 6. References - p.40 Chapter 7.Supplementary Figures - p.47 Supplementary figure.1. The equipment of itch-response scratching behavior - p.47 Supplementary figure.2. Histamine at higher concentration causes normal scratching behavior in HTR6 heterozygous KO mice - p.48 Supplementary Video.1. The demonstrating video of scratching behavior induced by higher concentration histamine between WT an HTR6 KO mice - p.49 | |
| dc.language.iso | en | |
| dc.title | HTR6在癢覺搔抓行為中所扮演之角色 | zh_TW |
| dc.title | The role of HTR6 on itch-associated scratching behavior | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳小梨(Show-Li Chen),曾明宗(Ming-Tsung Tseng) | |
| dc.subject.keyword | 傷害性感覺,癢覺,血清素,血清素受體亞型6型,脊椎背角,脊椎,搔抓行為, | zh_TW |
| dc.subject.keyword | nociception,itch,serotonin,HTR6,dorsal horn,spinal cord,scratching behavior, | en |
| dc.relation.page | 49 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2015-08-05 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 腦與心智科學研究所 | zh_TW |
| 顯示於系所單位： | 腦與心智科學研究所 | |
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