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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張惠婷 | |
| dc.contributor.author | Guan-Rong Chen | en |
| dc.contributor.author | 陳冠融 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:43:51Z | - |
| dc.date.copyright | 2015-08-11 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-11 | |
| dc.identifier.citation | 王松永 (1983) 商用木材。中華民國林產事業協會。台北市。pp. 86-90。
中央研究院生物多樣性研究中心 (2014) 臺灣物種名錄。中央研究院生物多樣性研究中心網址：http://taibnet.sinica.edu.tw/home.php 世界衛生組織 (2012) 實況報導─癌症。世界衛生組織網站：http://www.who.int/mediacentre/factsheets/fs297/zh/ 行政院衛生署食品藥物管理局 (2002) 癌症防治。行政院衛生署食品藥物管理局網址：http://www.fda.gov.tw/TC/index.aspx 行政院衛生署國民健康局 (2012) 肝癌是沉默殺手。行政院衛生署國民健康局網址：http://health99.doh.gov.tw/TXT/HealthyHeadlineZone/ HealthyHeadlineDetai.aspx?TopIcNo=6405 行政院衛生署統計室 (2014) 民國100年國人主要死因統計。行政院統計處網址：http://www.mohw.gov.tw/cht/DOS/ 吳佩芳 (2013) 遠離「肝炎--肝硬化--肝癌」肝病三部曲的魔咒。高醫醫訊月刊 32 (9): 23。 吳定國 (2013) 肝癌之治療--經導管肝動脈栓塞術。高醫醫訊月刊 32 (9): 20。 林務局 (2014) 森林資源調查專案成果：全島各縣市森林地面積及森林覆蓋率。林務局網址：http://www.forest.gov.tw/ct.asp?xItem=891&ctNode=1582&mp=1 國家中醫藥管理局（中華本草）編委會 (1999) 中華本草6。上海科學技術出版社。上海。pp. 466-467。 莊萬龍 (2013) 綜說肝病。高醫醫訊月刊 32 (9): 4。 陳信成 (2013) 肝癌之局部治療--純酒精注射與電燒。高醫醫訊月刊 32 (9): 19。 陳泰安 (2006) 肝炎與肝癌之防治。聲洋防癌之聲春季號 pp. 17-20。 黃鈞民和黃志仁 (2013) 放射線治療對肝癌有幫助嗎？高醫醫訊月刊 32 (9): 21。 楊少萱 (2002) 肝癌患者醫療成本分析-以某醫學中心患者為例。國立臺北醫學大學醫務管理學系碩士論文。pp. 4-8。 楊培銘（1998） 肝癌不再是絕症：當前對肝細胞癌之治療方法。肝癌防治會刊 5:10-13。 楊寧蓀、溫致群、王倩俁 (2010) 發炎、癌症、及以草藥防治之應用。中央研究院週報 1260: 7-9。 鄧富元、龔素芳、夏毅然、高文斌、謝耀東 (2006) NF-κB在發炎反應、細胞凋亡與癌症生成過程中的調控。中華牙醫學雜誌 25(1)：12-24。 謝明彥 (2013) 肝癌的標靶治療。高醫醫訊月刊 32 (9): 22。 王子定、郭寶章、袁強 (1979) 松鼠對臺灣林木之為害。國立臺灣大學國科會研究報告。 王子定、郭寶章 (1980) 松鼠對臺灣經濟林木之為害。科學發展月刊 8 (6):527-550。 伊肇基 (1957) 松鼠防治簡說。臺灣省政府農林廳林產管理局編印。 朱紀實 (1986) 臺灣杉、柳杉和杉木內皮化學成分與松鼠危害之相關性。國立臺灣大學森林學系研究所。碩士論文。第29頁。 邱健介 (1986) 臺灣杉粗樹脂抗松鼠機制之研究。國立臺灣大學森林學系碩士論文。第43頁。 張萬福 (1982) 臺灣赤腹松鼠食性與活動範圍。東海大學環境科學研究中心。 張誌益 (1999) 黃心柿莖部與臺灣杉樹皮之化學成份硏究。國立臺灣大學化學研究所博士論文。第17-30頁。 簡世昌 (2004) 臺灣杉皮部與臺灣扁柏心材之成分研究。國立臺灣大學化學研究所博士論文。第11-19頁。 謝興邦 (2007) 簡介癌症標靶治療藥物研究現況。國家衛生研究院電子報。第 199 期。 Aggarwal, B. B. and S. Shishodia (2006) Molecular targets of dietary agents for prevention and therapy of cancer. Biochemical Pharmacology 71: 1397 – 1421. American Cancer Society (2014) Liver cancer. American cancer society. America Atlanta pp. 1-66. Amiri, F., A. H. Zarnani, H. Zand, F. Koohdani, J. Tehrani Mahmood, and M. Vafa (2013) Synergistic anti-proliferative effect of resveratrol and etoposide on human hepatocellular and colon cancer cell lines. European Journal of Pharmacology 718: 34-40. Bai, N., K. He, Z. Zhou, M. L. Tsai, L. Zhang, Z. Quan, X. Shao, M. H. Pan, and C. T. Ho (2010) Ent-kaurane diterpenoids from rabdosia rubescens and their cytotoxic effects on uman cancer cell lines. Planta Medica 76: 140–145. Bharat, B. A., S. Shishodia, S. K. Sandur, M. K. Pandey, and G. Sethi (2006) Inflammation and cancer: How hot is the link? Biochem Pharmacol 72:1065-0621. Chang, C. I, J. Y. Chang, C. C. Kuo, W. Y. Pan, and Y. H. Kuo (2005) Four new abietane type diterpenes and antitumoral cytotoxic diterpene constituents from the bark of Taiwania cryptomerioides. Planta Medica 71: 72-76. Chang, S. T., P. F. Chen, S. Y. Wang, and H. H. Wu (2001) Antimite activity of essential oils and their constituents from Taiwania cryptomerioides. Journal of Medical Entomology 38 (3): 455-457. Chang, S.T., S. S. Cheng, and S. Y. Wang (2001) Antitermitic activity of essential oils and components from Taiwania (Taiwania crypotomerioids). Journal of Chemical Ecology 27 (4): 717-724. Christine, L. C. and R. A. Weinberg (2011) A perspective on cancer cell metastasis. Science 331: 1559-1564. Christopher, P. L., M. Witvrouw, M. P. De Bethune, M. T. Burch, H. F. Mower, H. Davis, A. Lasure, R. Pauwels , E. D. Clercq, and A. J. Vlletinck (1996) Antiviral activity of Hawaiian medicinal plants against human immunodeficiency virus type-1 (HIV-1). Phytomedicine 2 (3): 259-264. Cooper, G. M. (2000) The Cell: A Molecular Approach. 2nd edition. Sinauer Associates. Sunderland. Available from: http://www.ncbi.nlm.nih.gov/books/NBK9963/ Estela, I.G., J. A.Moran-Pinzona, L. G. Ortiz, D. Olmedo, E. del Olmo, J. L.López-Pérez, A. S. Feliciano, and M. P.Gupta (2010) Vasoactive effects of different fractions from two Panamanian plants used in Amerindian traditional medicine. Journal of Ethnopharmacology 131:497-501. Eufemio, M., T. Varughese, C. Spadafora, A. E. Arnold, P. D. Coley, T. A. Kursar, W. H. Gerwick, and L. C. Rios (2011) Chemical constituents of the new endophytic fungus Mycosphaerella sp. nov. and their anti-parasitic activity. Natural Product Communications 6(6): 835–840. Faedmaleki, F., F. H Shirazi, A.-A. Salarian, H. A. Ashtiani, and H. Rastegar (2014) Toxicity effect of silver nanoparticles on mice liver primary cell culture and Hep G2 cell line. Iranian Journal of Pharmaceutical Research 13 (1): 235-242. Fukumi, S., J. H.Yamada, S. Iwase, T. Ohno, and H. Yamada (2000) Concentration-dependent variable effects of etoposide on the cell cycle of CML cell. Anticancer Research 20 (5A) :3105-3010. Gagandeep, S., P. M. Novikoff, M. Ott, and S. Gupta (1999) Paclitaxel shows cytotoxic activity in human hepatocellular carcinoma cell lines. Cancer Letters 136: 109-118. Gugulethu, N., G. Fouche, M. Tselanyane, W. Cordier, and V. Steenkamp (2013) In vitro determination of the anti-aging potential of four southern African medicinal plants. BioMed Central Complementary and Alternative Medicine 13: 304-310. He, K., L. Zeng, G. Shi, G. X, Zhao, J. F. Kozlowski, and J. L. McLaughlin (1997) Bioactive compounds from Taiwania cryptomerioides Hayata. Journal of National Products 60: 38-40. Ho, P. J., C. K. Chou, Y. H. Kuo, L. C. Tu, and S. F. Yeh (2007) Taiwanin A induced cell cycle arrest and p53-dependent apoptosis in human hepatocellular carcinoma HepG2 cell. Life Sciences 80: 493–503. Ho, C. L., and Y. C. Su (2012) Composition, antioxidant and antimicrobial activities of the leaf essential oil of Machilus japonica from Taiwan. Natural Product Communications 7 (1): 109-112. Hsieh, T. J., T. Z. Liu, C. L. Chern, D. A. Tsao, Fung-Jou Lu, Y. H. Syu, P. Y. Hsieh, H. S. Hu, T. T. Chang, and C. H. Chen (2005) Liriodenine inhibits the proliferation of human hepatoma cell lines by blocking cell cycle progression and nitric oxide-mediated activation of p53 expression. Food and Chemical Toxicology 43: 1117–1126. Huang, G. J., J. S. Deng, S. S. Huang, C. I Chang, T. N. Chang, P. H. Shie, and Y. H. Kuo (2011) Anti-inflammatory activities of 6β-acetoxy-7α-hydroxyroyleanone from Taiwania cryptomerioides Hayata ex vivo and in vivo. Journal of Agricultural and Food Chemistry 59: 11211-11218. Jie, S., H. Li, Y. Tian, D. Guo, J. Zhu, S. Gao, and L. Jiang (2010) Berberine inhibits angiogenic potential of Hep G2 cell line through VEGF down-regulation in vitro. Journal of Gastroenterology and Hepatology 26: 79–185. Kafuku, K. and R. Kato (1931) On the essential oil of Taiwania cedar. Bulletin of The Chemical Society of Japan 27: 5337-5341. Kuo, Y. H., S. C. Chien, and C. C. Kuo (2002) Antioxidative 7-oxodehydropodocarpane-type trinorditerpenes from the bark of Taiwania cryptomerioides. Planta Medica 68: 1020-1023. Krysko, D. V., T. V. Berghe, K. D’Herde, and P. Vandenabeele (2008) Apoptosis and necrosis: Detection, discrimination and phagocytosis. Methods 44:205–221. Lam, K. L., K. L. Yang, E. Sunderasan, and M. T. Ong (2012) Latex C-serum from Hevea brasiliensis induces non-apoptotic cell death in hepatocellular carcinoma cell line (Hep G2). Cell Proliferation 45: 577–585. Lin, C. T., F. H. Chu2, S. T. Chang, P.J. Chueh, Y. C. Su, K.T. Wu, and S.Y. Wang (2007) Secoaggregatalactone A from Lindera aggregate induces apoptosis in human hepatoma Hep G2 cell. Planta Medica 73: 1548–1553. Liu, J., E. E. Puscheck, F. Wang, A. Trostinskaia, D. Barisic, G. Maniere, D. Wygle, W. Zhong, E. H. H. M. Rings, and D. A. Rappolee (2004) Serine-threonine kinases and transcription factors active in signal transduction are detected at high levels of phosphorylation during mitosis in preimplantation embryos and trophoblast stem cell. Reproduction 128: 643–654. Liu, T.Z., J. T. Cheng, S. J. Yiin, C. Y. Chen, C. H. Chen, M. J. Wu, and C. L. Chern (2008) Isoobtusilactone A induces both caspase-dependent and -independent apoptosis in Hep G2 cell. Food and Chemical Toxicology 46: 321–327. Locher, C. P., M. T. Burch, H. F. Mower, J. Berestecky, H. Davis, B. V. Poel, A. Lasure, D. A. V. Berghe, and A. J. Vlietinck (1995) Anti-microbial activity and anti-complement activity of extracts obtained from selected Hawaiian medicinal plants. Journal of Ethnopharmacology 49: 23-32. National Cancer Institute (2009) What you need to know about liver cancer. National Cancer Institute pp: 1-42. Nayeli, M. B., M. L. A. Ocampo, E. J. Ferrer, A. R. Ji. Aparicio, A. Zamilpa, M. G. Cortazar, . Tortoriello, and M. H. Ruiz (2013) Anti-Inflammatory activity of different agave plants and the compound cantalasaponin-1. Molecules 18: 8136-8146. Ng, K. W., S. M. Salhimi, A. M. S. A. Majid, and K. L. Chan (2010) Anti-angiogenic and cytotoxicity studies of some medicinal plants. Planta Medica 76: 935–940. Ogunwande, I. A., N. O. Olawore, O. O. Ogunmola, T. M. Walker, J. M. Schmidt, and W. N. Setzer (2007) Cytotoxic effects of Taxodium distichum oils. Pharmaceutical Biology 45 (2):106-110. Rao, M. M. and D. G. I. Kingston (1982) Plant anticancer agents. xii. isolation and structure elucidation of new cytotoxic quinones from Tabebuia cassinoides. Journal of Natural Products 45: 600-604. Ratnasooriya, W. D. and M. G. (1999) Dharmasiri, a water extract of leaf and stems of Psychotria sarmentosa has analgesic and antihyperalgesic activity in rats. Medical Science Research 27 (10): 715-718. Saha, K., N. H. Lajis, D. A. Israf, A. S. Hamzahd, S. Khozirah, S. Khamisa, and A. Syahida (2004) Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. Journal of Ethnopharmacology 92: 263-267. Samantha, L. G., R. Rathinakumar, G. Chakravarty, U. Göransson, W. C. WimLey, S. P. Darwin, and D. Mondal (2010) Anticancer and chemosensitizing abilities of cycloviolacin O2 from Viola odorata and Psyle cyclotides from Psychotria leptothyra. Peptide Science 94 (5): 617-625. Sarris, J., E. McIntyre, and D. A. Camfield (2013) Plant-based medicines for anxiety disorders, Part 2: A review of clinical studies with supporting preclinical evidence. Central Nervous System Drug 27: 301-319. Selvarajah, J., A. Elia, V. A. Carroll, and A. Moumen (2014) DNA damage-induced S and G2/M cell cycle arrest requires mTORC2-dependent regulation of Chk1. Oncotarget 6(1): 427-440. Shin, H. Y., S. H. Kim, H. J. Jeong, S. Y. Kim, T. Y. Shin, J. Y. Um, S. H. Hong, and H. M. Kim (2007) Epigallocatechin-3-gallate inhibits secretion of TNF-α, IL-6 and IL-8 through the attenuation of ERK and NF- κB in HMC-1 cell. Int Arch Allergy Immunol 142:335–344. Shyur, L. F., S. H. Leea, S. T. Chang, C. P. Lo, Y. H. Kuoa, and S. Y. Wang (2010) Taiwanin A inhibits MCF-7 cancer cell activity through induction of oxidative stress, upregulation of DNA damage checkpoint kinases, and activation of p53 and FasL/Fas signaling pathways. Phytomedicine 18: 16-24. Sleiman, R. J., D. R. Catchpoole, and B. W. Stewart (1998) Drug-induced death of leukaemic cell after G/M arrest: higher order DNA fragmentation as an indicator of mechanism. British Journal of Cancer 77(1): 40-50. Tao, Z., X. Ding, D. Wei, P. Cheng, X. Su, H. Liu, D. Wang, and H. Gao (2010) Sorafenib improves the survival of patients with advanced hepatocellular carcinoma: a meta-analysis of randomized trials. Anti-Cancer Drugs 21(3): 326 – 332. Tarsila, M. da S. M., M. H. de Araújo, N. R. Bernardes, D. B. de Oliveira, E. B. Lasunskaia, M. F. Muzitano, and M. D. Cunha (2011) Antimycobacterial activity and alkaloid prospection of Psychotria species (rubiaceae) from the brazilian atlantic rainforest. Planta Medica 77: 964–970. Toshimitsu, H., F. T. Smith, and K. H. Lee (1987) Antitumor agents. 89, psychorubrin, a new cytotoxic naphthoquinone from Psychotria rubra and its structure-activity relationships. Journal of Medicinal Chemistry 30 (11): 2005-2008. Yang, T. P., H. J. Lee, T. T. Ou, Y. J. Chang, and C. J. Wang (2012) Mulberry leaf polyphenol extract induced apoptosis involving regulation of adenosine monophosphate-activated protein kinase / fatty acid synthase in a p53-negative hepatocellular carcinoma cell. Journal of Agricultural and Food Chemistry 60: 6891−6898. Yang, X. R., Y. Y. Wang, K. K. La, L. Peng, X. H. Song, X. G. Shi, X. F. Zhu, P. C. Leung, C. H. Ko, and C. X. Ye (2012) Inhibitory effects of cocoa tea (Camellia ptilophylla) in human hepatocellular carcinoma Hep G2 in vitro and in vivo through apoptosis. Journal of Nutritional Biochemistry 23: 1051–1057. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17805 | - |
| dc.description.abstract | 本研究之目的為探討臺灣杉 (Taiwania cryptomerioides) 樹皮精油抑制Hep G2肝癌細胞株生長活性，期盼開發臺灣杉樹皮精油在醫療保健的新用途。以臺灣杉樹皮精油及其成分對Hep G2細胞進行細胞生長抑制評估，發現臺灣杉樹皮精油對Hep G2細胞具有顯著的細胞毒性，樹皮精油的6個分離部中，以S3分離部的細胞毒性最為顯著，S3分離部中的化合物S3A亦同樣具有顯著細胞毒性，效果優於正對照組Etoposide。以流式細胞儀分析經臺灣杉樹皮精油處理後的Hep G2細胞死亡型態，正對照組Etoposide、臺灣杉樹皮精油、S3分離部及化合物S3A處理後的組別，皆可使Hep G2細胞產生細胞凋亡。分析臺灣杉樹皮精油及其成分對Hep G2細胞的細胞週期影響，正對照組Etoposide主要作用於細胞的G2/M期，臺灣杉樹皮精油處理24 hr可輕微抑制Hep G2細胞的G0/G1期，S3分離部及化合物S3A可使Hep G2肝癌細胞G0/G1期的生長停滯。綜合上述結果，顯示臺灣杉樹皮精油及其成分可抑制Hep G2肝癌細胞的生長，具有抗肝癌細胞的潛力。 | zh_TW |
| dc.description.abstract | The aim of this study is to investigate the inhibition of Hep G2 cell (Human hepatocellular carcinoma cell) proliferation by Taiwania cryptomerioides bark oil. Through this study, it is expected that T. cryptomerioides bark oil can be used as pharmaceuticals or healthy products. Bark oil and its compounds are used to evaluate the cytotoxicity of Hep G2 cell. Bark oil has the significant cytotoxicity to Hep G2 cell. S3 fraction is the most effective fraction in cytotoxicity to Hep G2 cell among the 6 fractions. Compound S3A from S3 fraction also significantly induces the cytotoxicity in Hep G2 cell. The cytotoxicity of S3A was even better than that of positive control, etoposide. To confirm the cell death situation, apoptosis analysis of Hep G2 cell was analyzed via flow cytometry. Results showed that etoposide, bark oil, S3 fraction, and S3A induced apoptosis in Hep G2 cell. Effects of bark oil and its compound on the cell cycle distribution of Hep G2 cell were analyzed. Etoposide induced cell cycle arrest in G2/M phase in Hep G2 cell. Bark oil induced cell cycle arrest in G0/G1 phase in Hep G2 cell for 24 hr. S3 fraction and S3A induce cell cycle arrest in G0/G1 phase in Hep G2 cell. According to the above results, T. cryptomerioides bark oil and its compound can inhibit the proliferation of Hep G2 cell with the potential as an anticancer agent against the hepatocellular carcinoma. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:43:51Z (GMT). No. of bitstreams: 1 ntu-104-R01625035-1.pdf: 6747959 bytes, checksum: 38f320813d87dd8feb4d1fee934cec94 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 摘要 I
Abstract II 圖目錄 V 表目錄 X 壹、前言 1 貳、文獻回顧 3 一、癌症 3 (一) 癌細胞簡介及特性 3 (二) 肝癌之預防與治療 5 (三) 植物天然物抗肝癌機制 6 1. 促進細胞凋亡 9 2. 干擾細胞週期 17 3. 抑制血管新生與轉移 20 二、臺灣杉抽出成分研究 23 (一)、臺灣杉樹皮抽出物成分 24 (二)、臺灣杉樹皮抽出成分之生物活性 26 1. 臺灣杉樹皮樹脂的松鼠忌避活性 26 2. 臺灣杉樹皮抽出物抗癌生物活性 29 3. 臺灣杉樹皮抽出物的抗發炎活性 30 4. 臺灣杉樹皮抽出物抗氧化活性 36 參、材料與方法 38 一、試驗材料 38 (一)、試材 38 (二)、試驗藥品與藥劑 38 1. 藥品 38 2. 溶劑 38 3. 肝癌細胞 38 二、試驗方法 38 (一)、臺灣杉樹皮精油之萃取 38 (二)、臺灣杉樹皮精油成分分離及鑑定 39 (三)、細胞培養 39 (四)、細胞冷凍與解凍 39 (五)、細胞毒性試驗 40 (六)、細胞凋亡分析 40 (七)、細胞週期分析 41 (八)、統計分析 41 肆、結果與討論 42 一、 Hep G2細胞生長狀況及DMSO的許可劑量 42 二、臺灣杉樹皮精油、純露及熱水抽出物對Hep G2細胞之細胞毒性 44 三、臺灣杉樹皮精油及其成分對Hep G2細胞之細胞凋亡活性 54 四、臺灣杉樹皮精油及其成分對Hep G2細胞週期之影響 63 伍、結論 73 陸、參考文獻 74 | |
| dc.language.iso | zh-TW | |
| dc.title | 臺灣杉樹皮精油之抗肝癌細胞活性 | zh_TW |
| dc.title | Cytotoxicity of Taiwania cryptomerioides Bark Oil on Hepatocellular Carcinoma Cell | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳惠玲,張上鎮,張美鈴 | |
| dc.subject.keyword | 臺灣杉,Hep G2細胞,肝癌,細胞毒性,細胞凋亡,細胞週期停滯, | zh_TW |
| dc.subject.keyword | Taiwania cryptomerioides,Hep G2 cell,Human hepatocellular carcinoma,Cytotoxicity,Apoptosis,Cell cycle arrest, | en |
| dc.relation.page | 81 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2015-08-11 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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