請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17759
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 許輔 | |
dc.contributor.author | Mei-Ju Chen | en |
dc.contributor.author | 陳美如 | zh_TW |
dc.date.accessioned | 2021-06-08T00:43:06Z | - |
dc.date.copyright | 2015-09-17 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-13 | |
dc.identifier.citation | 王竹鑫、章迺榮(民95)。袖珍中藥快速鑑別手冊。長沙:湖南科學技術出版社。
于龍順、趙國學、任世蘭、楊教文、夏敬生、嚴丹方、李曉蘭(民78)。天麻抗炎效應的研究。中草藥,20(5),19-21。 李慶業(民95)。方劑學。北京:中國醫藥出版社。 李婉慈(民99)。探討台灣金線連免疫調節蛋白對小鼠腹腔巨噬細胞之活化作用 (未出版之碩士論文)。國立臺灣大學,臺北市。 沈立言(民98)。天麻水萃物在動物實驗及臨床試驗中的抗憂鬱效果及其機制之 探討。中醫藥年報,27(1),279-314。 行政院衛生署編印(民93)。中華中藥典。行政院衛生署中醫藥委員會編修小組。 台北。 邱德文(民95)。方劑學。貴州:貴州人民出版社。 林世航(民101)。以蛋白質體學探討天麻水萃物抗憂鬱之功效(未出版之碩士論 文)。國立臺灣大學,臺北市。 徐錦堂(民82)。中國天麻栽培學。北京:北京醫科大學中國協和醫科大學聯合 出版社。 陳怡敏(民77)。天麻注射液對小鼠免疫功能的影響。上海免疫學雜誌,8(5), 337-338。 許如君(民94)。台灣金線連免疫調節蛋白 IPAF 活化免疫細胞之機制(未出版 之碩士論文)。國立臺灣大學,臺北市。 張賢哲、蔡貴花(民73)。中藥炮製學,初版。台中:中國醫藥學院出版組。 項平(民83)。中醫食療方全錄。北京:人民衛生出版社 黃正良(民74)。我國天麻的藥理研究及臨床應用現狀。中西醫結合雜誌,5, 251-254。 黃順爵(民88)。天麻之生藥鑑定、成分分析與降血壓作用研究(未出版之碩士 論文)。中國醫藥學院,臺中市。 謝明村(民83)。抗衰老中藥藥理活性之研究。行政院國家科學委員會專題研究 計畫成果報告,未出版。 嚴正華(民92)。中藥學(下),第一版。臺北:知音出版社。 Ahn, E. K., Jeon, H. J., Lim, E. J., Jung, H. J., Park, E. H. (2007). Anti- inflammatory and anti-angiogenic activities of Gastrodia elata Blume. J Ethnopharmacol, 110, 476-482. An, S. J., Park, S. K., Hwang, I. K., Choi, S. Y., Kim, S. K., Kwon, O. S., Jung, S. J., Baek, N. I., Lee, H. Y., Won, M. H., Kang, T. C. (2003). Gastrodin decreases immunoreactivities of gamma-aminobutyric acid shunt enzymes in the hippocampus of seizure-sensitive gerbils. J Neurosci Res, 71, 534-543. Andersson, M., Bergendorff, O., Nielsen, M., Sterner, O., Witt R, A. J., Lu, A., Wang , A. M. (1995). Inhibition of kainic acid binding to glutamate receptors by extracts of Gastrodia. Phytochemistry. 38(4), 835-836. Banchereau, J., Briere F, C. C., Davoust, J., Lebeque, S., Liu, Y. J., Pulendran, B., Palucka, K. (2000). Immunobiology of dendritic cells. Annu. Rev. Immunol. 18, 767-811. Bliss K, B. B., Denkers, E. (2000). Rapid recruitment of neutrophils containing prestored IL-12 during microbial infection. J. Immunol. 165, 4515-4521. Bredt, D.S., Hwang, P. M., Glatt, C. E., Lowenstein, C., Reed, R. R., Snyder, S. H. (1991). Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature. 351, 714-718. Cao, Y., Zhang, X., Fang, Y., Ye, J. (2001). Determination of the active ingredients in Gastrodia rhizoma by capillary electrophoresis with electrochemical detection. Analyst. 126, 1524-1528. Chen, P. J., Sheen, L. Y. (2011). Gastrodiae Rhizoma (天麻tiān má): a review of biological activity and antidepressant mechanisms. J. Tradit. Complement. Med. 8, 31-40. Gutierrez RMP. (2010). Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology. J. Med. Plants Res. 4, 592-638. Ha, J. H., Lee, D. U., Lee, J. T., Kim, J. S., Yong, C. S., Kim, J. A., Ha, J. S., Huh, K. (2000). 4-Hydroxybenzaldehyde from Gastrodia elata B1 is active in the antioxidation and GABAergic neuromodulation of the rat brain. J. Ethnopharmacol. 73, 329-333 Hibbs, J. B., Taintor, R. R., Vavrin, Z. (1987). Macrophage cytotoxicity: Role of L-arginine deiminase and imino nitrogen oxidation to nitrite. Science. 235, 473-476. Higuchi, M., Higashi, N., Taki, O. T. (1990). Cytolytic mechanisms of activated macrophages: Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. J. Immunol. 144, 1245-1251. Hsieh, M. T., Wu, C. R., Chen, C. F. (1997). Gastrodin and p-hydroxybenzyl alcohol facilitate memory consolidation and retrieval, but not acquisition, on the passive avoidance task in rats. J. Ethnopharmacol. 56, 45-54. Hsieh, C. L., Chang, C. H., Chiang, S.Y., Li, T.C., Tang, N.Y., Pon, C. Z., Hsieh, C. T., Lin, J. G. (2000). Anticonvulsive and free radical scavenging activities of vanillyl alcohol in ferric chloride-induced epileptic seizures in Sprague-Dawley rats. Life Sci. 67,1185-1195. Hsieh, M. T., Peng, W. H., Wu, C. R., Wang, W. H. (2000). The ameliorating effects of the cognitive-enhancing Chinese herbs on scopolamine-induced amnesia in rats. Phytother. Res. 14, 375-377. Hsieh, C. L., Chiang, S. Y., Cheng, K. S., Lin, Y. H., Tang, N.Y., Lee, C. J., Pon, C. Z., Hsieh, C. T. 2001. Anticonvulsive and free radical scavenging activities of Gastrodia elata Bl. in kainic acid-treated rats. Am. J. Chin Med. 29, 331-341. Hsieh, C. L., Chen , C. L., Tang, N. Y., Chuang, C. M., Hsieh, C. T., Chiang, S.Y., Lin, J.G., Hsu, S. F. (2005). Gastrodia elata B1. mediates the suppression of nNOS and microglia activation to protect against neuronal damage in kainic acid-treated rats. Am. J. Chin Med. 33, 599-611. Jung, J. W., Yoon, B. H., Oh, H. R., Ahn, J. H., Kim, S. Y., Park, S.Y., Ryu, J. H. (2006). Anxiolytic-like effects of Gastrodia elata and its phenolic constituents in mice. Biol. Pharm Bull. 29(2), 261-265. Karupiah, G., Xie, Q. W., Buller, R. L., Nathan, C., Duarte, C. M, Micking, J. D. (1993). Inhibition of viral replication by interferon-γ-induced nitric oxide synthase. Science 261, 1445-1448. Kim, B.W., Koppula, S., Kim, J. W., Lim, H.W., Hwang, J. W., Kim, I. S., Park, P. J., Choi, D. K. (2012). Modulation of LPS-stimulated neuroinflammation in BV-2 microglia by Gastrodia elata: 4-Hydroxybenzyl alcohol is the bioactive candidate. J. Ethnopharmacology. 139, 549-557. Kim, H. J., Moon, K. D., Oh, S. Y., Kim, S. P., Lee, S.R. (2001). Ether fraction of methanol extracts of Gastrodia elata, a traditional medicinal herb, protects against kainic acid-induced neuronal damage in the mouse hippocampus. Neurosci Lett. 314,65-68. Kim, H. J., Lee, S. R., Moon, K. D. (2003a). Ether fraction of methanol extracts of Gastrodia elata, medicinal herb protects against neuronal cell damage after transient global ischemia in gerbils. Phytother Res. 17, 909-912. Kim, H. J., Moon, K. D., Lee, D. S., Lee, S. H. (2003b). Ethyl ether fraction of Gastrodia elata Blume protects amyloid beta peptide-induced cell death. J. Ethnopharmacol. 84, 95-98. Kuan, Y. C., Wu, T. J., Kuo, C. Y., Hsu, J. C., Chang, W. Y., Sheu, F. (2011). Molecular cloning of a new immunomodulatory protein from Anoectochilus formosanus which induces B cell IgM secretion through a T-independent mechanism. PLOS ONE 6(6),e21004. Lee, Y. S., Ha, J. H., Yong, C.S., Lee, D. U., Huh, K., Kang, Y. S., Lee, S. H., Jung, M. W., Kim, J. A. (1999). Inhibitory effects of constituents of Gastrodia elata Bl. On glutamate-induced apoptosis in IMR-32 human neuroblastoma cells. Arch Pharm Res.22(4), 404-409. Liu, J., Mori, A. (1992). Antioxidant and free radical scavenging activities of Gastrodia elata Bl. and Uncaria rhynchophylla (Miq.) Jacks. Neuropharmacology. 31, 1287-1298. Liu, J., Mori, A. (1993). Antioxidant and pro-oxidant activities of p-hydroxybenzyl alcohol and vanillin: effects on free radicals, brain peroxidation and degradation of benzoate, deoxyribose, amino acids and DNA. Neuropharmacology. 32, 659. MacMicking, J. D., Nathan, C., Hom, G., Chartrain, N., Fletcher, D., Trumbauer, M., Stevens, K., Xie, Q. W., Sokol, K., Hutchinson, N., Chen, H., Mudgett, J.S. (1995). Altered responses to bacterial infection and endotoxic shock in mice lacking inducible nitric oxide synthase. Cell 81, 641-650. Mori, A., Yokoi, I., Noda, Y., Willmore, L. J. (2004). Natural antioxidants may prevent posttraumatic epilepsy: a proposal based on experimental animal studies. Acta Med Okayama. 58, 111-118. Wick, J. (2004). Living in the danger zone: innate immunity to Salmonella. Curr. Opin. Microbiol. 7, 51-57. Ojemann, L. M., Nelson, W. L., Shin, D. S., Rowe, A. O., Buchanan, R. A. (2006). Tianma, an ancient Chinese herb, offers new options for the treatment of epilepsy and other conditions. Epilepsy Behavio . 8, 376-383. Raychaudhuri, B., Dweik, R. A., Connors, M., Buhrow, L., Malur, A., Drazba, J., Arroliga, A. C., Erzurum, S.C., Kavuru, M., Thomassen, M. J. (1999). Nitric oxide blocks nuclear factor-κB activation in alveolar macrophage. Am. J. Respir. Cell Mol. Biol. 21, 311-316. Siebenlist , U., Franzoso, G., Brown, K. (1994). Structure, regulation and function of NF-kappa B. Annu. Rev. Cell Biol. 10, 405-455. Stuehr, D., Nathan, C. F. (1989). Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells. J. Exp. Med. 169, 1543-1555. Xie, Q. W., Cho, H., Calaycay, J., Mumford, R. A., Swiderek, K. M., Lee, T. D., Ding, A., Troso, T., Nathan, C. (1992). Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256, 225-228. Xie, Q. W., Cho, H., Kashiwabara, Y., Baum, M., Weidner, J. R., Elliston, K., Mumford, R., Nathan, C. (1994). Carboxyl terminus of inducible nitric oxide synthase: Contribution to NADPH binding and enzymatic activity. J. Biol. Chem. 269,28500-28505. Wick J. (2004). Living in the danger zone : innate immunity to Salmonella. Curr. Opin. Microbiol. 7,51-57. Wu, C. R., Hsieh, M. T., Huang, S. C., Peng, W. H., Chang, Y. S., Chen, C. F. (1996a). Effects of Gastrodia elata and its active constituents on scopolamine-induced amnesia in rats. Planta Med. 62, 317-321. Wu, C. R., Hsieh, M. T., Liao, J. (1996b). p-Hydroxybenzyl alcohol attenuates learning deficits in the inhibitory avoidance task: involvement of serotonergic and dopaminergic systems. Chin J. Physiol. 39, 265-273. Xul, Q., Lid, Y., Wang, X., Gu, H., Chen, Z. (1998). Purification and characterization of a novel anti-fungal protein from Gastrodia elata. Plant Physiology Biochemistry. 36(12),899-905. Zhou, B. H., Li, X. J., Liu, M., Wu, Z., Ming, Hu. X. (2006). Antidepressant-like activity of the Gastrodia elata ethanol extract in mice. Fitoterapia . 77, 592-594. Zhu, W., Ma, S., Qu, R., Kang, D. (2006). Antidepressant-like effect of saponins extracted from Chaihu-jia-longgu-muli-tang and its possible mechanism. Life Sci. 79, 749-756. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17759 | - |
dc.description.abstract | Tianma (Gastrodia elata), also known as fixed wind grass or barbed arrows, is a medicinal orchid used in traditional Chinese medicine to treat vertigo, paralysis, convulsions, cramps, epilepsy, high blood pressure and other symptoms. We have previously purified an immunomodulatory protein IPAF from Anoectochilus formosanus. The protein IPAF has an amino acid sequence highly similar to proteins from Orchidaceae plants, including Epipactis helleborine lectin, Listera ovata lectin, and G. elata-gastrodianin-4B. Therefore, we hypothesized that Orchidaceae plants might possess proteins similar to IPAF. To test our hypothesis, the crude protein was extracted from G. elata, Dendrobium huoshanense, Dendrobium nobile, and Dendrobium loddigesii and then analyzed by polyacrylamide gel electrophoresis and Western blotting. Subsequently, a 15.1 kDa protein G. elata (GEP), which exhibited similar biochemical characteristics to IPAF, was purified via anion exchange chromatography using DE-52 and HiTrap Q columns. Finally, RAW 264.7 murine macrophage cells were used for preliminary activity tests. GEP (5 μg/mL) could activate nitric oxide (NO) (10.4 ± 0.6 μM), TNF-α (935.4 ± 118.5 pg/mL), and IL-1β (25.1 ± 3.0 pg/mL) secretion by RAW 264.7 murine macrophage. In addition, GEP enhanced nucleic acid replication by 1.16-fold as analyzed by BrdU analysis, and promoted 1.43-fold of MTT metabolic activity in murine splenocytes. In this study we purified a new orchid protein, which exhibited similar biochemical characteristics as the IPAF, and we also assessed its immunomodulatory function. GEP activated RAW 264.7 macrophages by enhancing NO, TNF-α and IL-1β secretion. Moreover, GEP also stimulated cell proliferation in mouse splenocytes. On the basis of previous the findings, in our laboratory GEP, as well as other orchid proteins, might play important roles in the medicinal functions of Orchidaceae plants. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T00:43:06Z (GMT). No. of bitstreams: 1 ntu-104-R00628214-1.pdf: 1577974 bytes, checksum: e6f38d6d4e67172242552035753c95a7 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 誌謝 I
摘要 III Abstract IV 目錄 VI 圖目錄 IX Chapter 1 緒論 1 1.1 序言 1 1.1.1 天麻典故 1 1.1.2 天麻之分部和栽培 1 1.1.3 天麻之傳統藥理與藥用潛力 3 1.1.4 天麻之生理活性 3 1.2 台灣金線連 8 1.3 免疫調節 10 1.3.1 免疫功能 10 1.3.2 發炎反應 11 1.3.3 巨噬細胞的免疫系統角色 12 1.4 研究動機與架構 16 Chapter 2 材料與方法 17 2.1 天麻、霍山石斛、川石斛和金釵石斛等四種蘭科植物粗蛋白製備 17 2.1.1 天麻、霍山石斛、川石斛和金釵石斛等四種蘭科植物粗蛋白萃取 17 2.1.2 天麻、霍山石斛、川石斛和金釵石斛粗蛋白之特性分析 19 2.2 天麻蛋白 GEP 純化 23 2.2.1 DE-52 離子交換樹脂層析 23 2.2.2 快速蛋白質層析系統(FPLC SYSTEM)分析 25 2.3 天麻蛋白 GEP 免疫調節活性試驗 25 2.3.1 一氧化氮 (NITRIC OXIDE) 產量分析 25 2.3.2 Α腫瘤壞死因子 (TNF-α) 產量之分析 27 2.3.3 介白質 (IL-1β) 產量之分析 28 2.3.4 小鼠脾細胞之取得 29 2.3.5 脾臟細胞的代謝活性 (MTT ASSAY) 29 2.3.6 脾臟細胞的細胞增生效應 (BRDU ASSAY) 30 2.3.7 Γ干擾素 (IFN-γ)產量之分析 31 Chapter 3 結果 32 3.1 四種藥用蘭科植物全蛋白萃取及特性分析 32 3.1.1 聚丙烯醯胺膠體電泳分析 32 3.1.2 西方轉漬分析 32 3.2 天麻蛋白 GEP 的純化 33 3.2.1 DE-52陰離子交換樹脂 33 3.2.2 快速蛋白層析系統 (FPLC SYSTEM) 分析 33 3.3 天麻蛋白 GEP 的免疫調節活性 34 3.3.1 天麻蛋白 GEP 對 RAW 264.7 MACROPHAGES 之影響 34 3.3.2 天麻蛋白 GEP 對小鼠脾細胞之影響 38 Chapter 4 討論 42 Chapter 5 結論 45 參考文獻 47 圖表 55 | |
dc.language.iso | zh-TW | |
dc.title | 天麻免疫調節蛋白之純化與活性分析 | zh_TW |
dc.title | Purification and Function Analysis of the Immunomodulatory Protein from Gastrodia elata | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 周志輝,潘敏雄 | |
dc.subject.keyword | 天麻,純化,免疫調節蛋白, | zh_TW |
dc.subject.keyword | Gastrodia elata,Purification,Immunomodulatory Protein, | en |
dc.relation.page | 66 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2015-08-13 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 1.54 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。