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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王愛玉 | |
dc.contributor.author | Bing-Heng Lee | en |
dc.contributor.author | 李秉衡 | zh_TW |
dc.date.accessioned | 2021-06-08T00:31:16Z | - |
dc.date.copyright | 2013-07-25 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-07-02 | |
dc.identifier.citation | Abid, G., Silue, S., Muhovski, Y., Jacquemin, J.M., Toussaint, A., and Baudoin, J.P. (2009). Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development. Gene 439, 1-10.
Albrecht, G., and Mustroph, A. (2003). Localization of sucrose synthase in wheat roots: increased in situ activity of sucrose synthase correlates with cell wall thickening by cellulose deposition under hypoxia. Planta 217, 252-260. Amor, Y., Haigler, C.H., Johnson, S., Wainscott, M., and Delmer, D.P. (1995). A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc Natl Acad Sci USA 92, 9353-9357. Andersen, M.N., Asch, F., Wu, Y., Jensen, C.R., Naested, H., Mogensen, V.O., and Koch, K.E. (2002). Soluble invertase expression is and early target of drought stress during the critical, abortion-sensitive phase of young ovary development in maize. Plant Physiol 135, 591-604. Angeles-Nunez, J.G., and Tiessen, A. (2010). Arabidopsis sucrose synthase 2 and 3 modulate metabolic homeostasis an direct carbon towards starch synthesis in developing seeds. Planta 232 ,701-718. Anguenot, R. Nguyen-Quoc, B., Yelle, S., and Michaud, D. (2006). Protein phosphorylation and membrane association of sucrose synthase in developing tomato fruit. Plant Physiol Biochem 44, 294-300. Anguenot, R., and Nguyen-Quoc, B. (1999). Purification of tomato sucrose synthase phosphorylated isoforms by Fe(III)-immobilized metal affinity chromatography. Arch Biochem Biophys 365, 163-169. Aoki, N., Scofield, G.N., Wang, X.D., Offler, C.E., Patrick, J.W., and Furbank, R.T. (2006). The pathway of sugar transport in germinating wheat seeds. Plant Physiol Preview 141, 1255–1263. Baroja-Fernandez, E., Munoz, F.J., Saikusa, T., Rodriguez-Lopez, M., Akazawa, T., and Pozueta-Romero, J. (2003). Sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants. Plant Cell Physiol 44, 500-509. Barratt, D.H., Barber, L., Kruger, N.J., Smith, A.M., Wang, T.L., and Martin, C. (2001). Multiple, distinct isoforms of sucrose synthase in pea. Plant Physiol 127, 655-664. Barratt, D.H., Derbyshire, P., Findlay, K., Pike, M., Wellner, N., Lunn, J., Feil, R., Simpson, C., Maule, A.J., and Smith, A.M. (2009). Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase. Proc Natl Acad Sci USA 106, 13124-13129. Baud, S., Vaultier, M.N., and Rochat, C. (2004). Structure and expression profile of the sucrose synthase multigene family in Arabidopsis. J Exp Bot 55, 397-409. Bertin, P., Gallais, A. (2001). Genetic variation for nitrogen use efficiency in a set of recombinant inbred lines. II - QTL detection and coincidence. Maydica 46, 53-68. Bieniawska, Z., Barratt, D.H.P., Garlick, A.P., Thole, V., Kruger, N.J., Martin, C., Zrenner, R., and Smith, A.M. (2007). Analysis of the sucrose synthase gene family in Arabidopsis. Plant J 49, 810-828. Bologa, K.L., Fernie, A.R., Leisse, A., Loureiro, M.E., and Geigenberger, P. (2003). A bypass of sucrose synthase leads to low internal oxygen and impaired metabolic performance in growing potato tubers. Plant Physiol 132, 2058-2072. Cardini, C.E., Leloir, L.F., and Chiriboga, J. (1955). The biosynthesis of sucrose. J Biol Chem 214, 149-155. Carlson, S.J., Chourey, P.S., Helentjaris, T., and Datta, R. (2002). Gene expression studies on developing kernels of maize sucrose synthase (SuSy) mutants show evidence for a third SuSy gene. Plant Mol Biol 49, 15-29. Castleden, C.K., Aoki, N., Gillespie, V.J., MacRae, E.A., Quick, W.P., Buchner, P., Foyer, C.H., Furbank, R.T., and Lunn, J.E. (2004). Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. Plant Physiol 135, 1753-1764. Chan, H.Y., Ling, T.Y., Juang R.H., Ting, I.N., Sung, H.Y., and Su, J.C. (1990). Sucrose synthase in rice plants - growth-associated changes in tissue specific distributions. Plant Physiol 94, 1456-1461. Chavez-Barcenas, A.T., Valdex-Alarcon, J.J., Martinez-Trujillo, M., Chen, L., Xoconostle-Cazares, B., Lucas, W.J., and Herrera-Estrella, L. (2000). Tissue-specific and developmental pattern of expression of the rice sps1 gene. Plant Physiol 124, 641-653. Chen A., He S., Li F., Li Z., Ding M., Liu Q., Rong J.. (2012) Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns. BMC Plant Biol 12, 85-101. Chen, Y.C., and Chourey, P.S. (1989). Spatial and temporal expression of the two sucrose synthase genes in maize: Immunohistological evidence. Theor Appl Genet 78, 553-559. Chen, H.Y., Ling, T.Y., Juang, R.H., Ting, I.N., Sung, H.Y., and Su, J.C. (1990). Sucrose synthase in rice plants. Plant Physiol 94, 1456-1461. Cheng, W.H., Endo, A., Zhou, L., Penney, J., Chen, H.C., Arroyo, A., Leon, P., Nambara, E., Asami, T., Seo, M., Koshiba, T., and Sheen, J. (2002). A unique shortchain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell 14, 2723-2743. Chiu, W.B., Lin, C.H., Chang, C.J., Hsieh, M.H., and Wang, A.Y. (2006). Molecular characterization and expression of four cDNAs encoding sucrose from green bamboo Bambusa oldhamii. New Phytol 170, 53-63. Chourey, P.S. (1981). Genetic control of sucrose synthase in maize endosperm. Mol Gen Gent 184, 372-376. Chourey, P.S., Latham, M., and Still, P.E. (1986). Expression of two sucrose synthetase genes in endosperm and seedling cells of maize: evidence of tissue specific polymerization of promoters. Mol Gen Gent 103, 251-255. Chourey, P.S., Taliercio, E.W., Carlson, S.J., and Ruan, Y.L. (1998). Genetic evidence that the two isozymes of sucrose synthase present in developing maize endosperm are critical, one for cell wall integrity and the other for starch biosynthesis. Mol Gen Genet 259, 88-96. Chiou, T.J., and Bush, D.R. (1998). Sucrose is a signal molecule in assimilate partitioning. Proc Natl Acad Sci USA 95, 4784-4788. Coleman, H.D., Yan, J., and Mansfield, S.D. (2009). Sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure. Proc Natl Acad Sci USA 106, 13118-13123. Denyer, K., Dunlap, F., Thorbjornsen, T., Keeling, P., and Smith, A.M. (1996). The major form of ADP-glucose pyrophosphorylase in maize endosperm is extra-plastidial. Plant Physiol 112, 779-785. Dorak, M.T. (ed.) (2006). Real-time PCR. UK: Taylor & Francis Group. Duncan, K.A., Hardin, S.C., Huber, S.C. (2006). The three maize sucrose isoforms differ in distribution, localization, and phosphorylation. Plant Cell Physiol 47, 959-971. Echt, C.S., and Chourey, P.S. (1985). A comparison of two sucrose synthase isozymes from normal and shrunken-1 maize. Plant Physiol 79, 530-536. Elion, E.A. (1999). Detection of protein-protein interactions by copreciptation. Current Protocols in Protein Science, published by John Wiley & Sons, 19.4.1-19.4.9. Elling, L., Grothus, M. and Kula, M.R. (1993). Investigation of sucrose synthase from rice for the synthesis of various nucleotide sugar and saccharides. Glycobiology 3, 349-355. Fallahi, H., Scofield, G.N., Badger, M.R., Chow, W.S., Furbank, R.T., and Ruan, Y.L. (2008). Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development. J Exp Bot 59, 3283-3295. Feng, Q., Zhang, Y., Hao, P., et al. (2002). Sequence and analysis of rice chromosome 4, Nature 420, 316-320. Fu, H., and Park, W.D. (1995). Sink- and vascular-associated sucrose synthase functions are encoded by different gene classes in potato. Plant Cell 7, 1369-1385. Fujii, S., Hayashi, T., and Mizuno, K. (2010). Sucrose synthase is an integral component of the cellulose synthesis machinery. Plant Cell Physiol 51, 294-301. Geigenberger, P., and Stitt, M. (1993). Sucrose synthase catalyses a readily reversible reaction in vivo in developing potato tubers and other plant tissues. Planta 189, 329-339. Hanggi, E., and Fleming, A.J. (2001). Sucrose synthase expression pattern in young maize leaves: implications for phloem transport. Planta 214, 326-329. Hardin, S.C., and Huber, S.C. (2006). Proteasome activity and the post-translational control of sucrose synthase stability in maize leaves. Plant Physiol Biochem 42, 197-208. Hiei, Y., Ohta, S., Komari, T., and Kumashiro, T. (1994). Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6, 271-282. Hirose, T., Scofield, G.N., and Terao, T. (2008). An expression analysis profile for the entire sucrose synthase gene family in rice. Plant Sci 174, 534-543. Horst, I., Welham, T., Kelly, S., Kaneko, T., Sato, S., Tabata, S., Parniske, M., and Wang, T.L. (2007). Tilling mutants of lotus japonicus reveal that nitrogen assimilation and fixation can occur in the absence of nodule-enhanced Sucrose synthase. Plant Physiol 144, 806-820. Huang, J.W., Chen, J.T., Yu, W.P., Shyur, L.F., Wang, A.Y., Sung, H.Y., Lee, P.D., and Su, J.C. (1996). Complete structures of three rice sucrose synthase isogenes and differential regulation of their expressions. Biosci Biotechnol Biochem 60, 233-239. Huang, D.Y., and Wang, A.Y. (1998). Purification and characterization of sucrose synthase isozymes from etiolated rice seedlings. Biochem Mol Biol Int 46, 107-113. Hubbard, N.L., Huber, S.C. and Pharrr, D.M. (1989). Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing mushmelon (Cucumis melo L.) fruit. Plant Physiol 91, 1527-1534. Huber, S.C., and Huber, J.L. (1996). Role and regulation of sucrose- phosphate synthase in higher plants. Annu Rev Plant Physiol Plant Mol Biol 47, 431-444. Huber, S.C., Huber, J.L., Liao, P.C., Gage, D.A., McMichael, R.W., Jr., Chourey, P.S., Hannah, L.C., and Koch, K. (1996). Phosphorylation of serine-15 of maize leaf sucrose synthase. Occurrence in vivo and possible regulatory significance. Plant Physiol 112, 793-802. Ishimaru, K., Ono, K., and Kashiwagi, T. (2003). Identification of a new gene controlling plant height in rice using the candidate-gene approach. Planta 218, 388-395. Kikuchi, S., Satoh, K., Nagata, T., et al. (2003). Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science 301, 376-370. Koch, K.E., Nolte, K.D., Duke, E.R., McCarty, D.R., and Avigne, W.T. (1992). Sugar levels modulated differential expression of maize sucrose synthase genes. Plant Cell 4, 59-69. Koch, K.E. (1996). Carbohydrate-modulated gene expression in plant. Ann Rev Plant Physiol Plant Molec Biol 47, 509-540. Koch, K.E. (2004). Sucrose metabolism: regulatory mechanism and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7, 235-246. Koch, K.E., Wu, Y., and Xu, J. (1996). Sugar and metabolic regulation of genes for sucrose metabolism: Potential influence of maize sucrose synthase and soluble invertase responses on carbon partitioning and sugar sensing. J Exp Bot 47, 1179-1185. Koch, K.E., and Zeng, Y. (2002). Molecular approaches to altered C partitioning: genes for sucrose metabolism. J Amer Soc Hort Sci 127, 474-483. Komatsu, A., Moriguchi, T., Koyama, K., Omura, M., and Akihama, T. (2002). Analysis of sucrose synthase genes in citrus suggests different roles and phylogenetic relationships. J Exp Bot 53, 61-71. Konishi, T., Ohmiya, Y., and Hayashi, T. (2004). Evidence that sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem. Plant Physiol 134, 1146-1152. Langenkamper, G., Fung, R.W.M., Newcomb, R.D., Atkinson, R.G., Gardner, R.C., and MacRae, E.A. (2002). Sucrose phosphate synthase genes in plants belong to three different families. J Mol Evol 54, 322-332. Larsen, A.E., Salerno, G.L., and Pontis, H.G. (1985). Sucrose synthase from wheat leaves. Comparison with the wheat germ enzyme. Physiol Plant 67, 37-42. Li J., Baroja-Fernandez E., Bahaji A., Munoz F.J., Ovecka M., Montero M., Sesma M.T., Alonso-Casajus N., Almagro G., Sanchez-Lopez A.M., Hidalgo M., Zamarbide M., Pozueta-Romero J. (2013). Enhancing sucrose synthase activity results in increased levels of starch and ADP-glucose in maize (Zea mays L.) seed endosperms. Plant Cell Physiol 54, 282-94. Li, Z.W., and Trick, H.N. (2005). Rapid method for high-quality RNA isolation from seed endosperm containing high levels of starch. BioTechniques 38, 872-876. Martin, T., Frommer, W.B., Salanoubat, M., and Willmitzer, L. (1993). Expression of an Arabidopsis sucrose synthase gene indicates a role in metabolization of sucrose both during phloem loading and in sink organs. Plant J 4, 367-377. Mishra P., Dubey R.S.. (2013). Excess nickel modulates activities of carbohydrate metabolizing enzymes and induces accumulation of sugars by upregulating acid invertase and sucrose synthase in rice seedlings. Biometals 26, 97-111. Moore, B., Zhou, L., Rolland, F., Hall, Q., Cheng, W.H., Liu, Y.X., Hwang, I., Jones, T., and Sheen, J. (2003). Role of the Arabidopsis glucose sensor HXK1 in nutrient, light, and hormonal signaling. Science 300, 332-336. Murata, T. (1972). Sucrose synthetase of rice grain and potatoes. Agric Biol Chem 36, 1815-1818. Munoz, F.J., Baroja-Fernandez, E., Moran-Zorzano, M.T., Viale, A.M., Etxeberria, E., Alonso-Casajus, N., and Pozueta-Romero J. (2005). Sucrose synthase controls both intracellular ADP glucose levels and transitory starch biosynthesis in source leaves. Plant Cell Physiol 46, 1366-1376. Nakai, T., Tonouchi, N., Konishi, T., Kojima, Y., Tsuchida, T., Yoshinaga, F., Sakai, F., and Hayashi, T. (1999). Enhancement of cellulose production by expression of sucrose synthase in acetobacter xylinum. Proc Natl Acad Sci USA 96, 14-18. Neubohn, B., Gubatz, S. Wobus, U., and Weber, H. (2000). Sugar levels altered by ectopic expression of a yeast-derived invertase affect cellular differentiation of developing cotyledons of Vicia narbonensis L. Planta 211, 325-334. Nomura, T., and Akazawa, T. (1973). Enzymic mechanism of starch synthesis in ripening rice grains. VII. Purification and enzymic properties of sucrose synthetase. Arch Biochem Biophys 156, 644-652. Persia, D., Cai, G., Casino, C.D., Faleri, C., Willemse, M.T.M., and Cresti, M. (2008). Sucrose synthase is associated with the cell wall of tobacco pollen tubes. Plant Physiol 147, 1603-1618. Peterson N.V., Astaf'Eva E.V. (1962). On the method of determination of saccharase activity in the soil. Mikrobiologiia 31, 918-922. Pozueta-Romero, J., Yamaguchi, J., and Akazawa, T. (1991). ADPG formation by the ADP-specific cleavage of sucrose-reassessment of sucrose synthase. FEBS Lett 291, 233-237. Ricard, B., Toai, T.V., Chourey, P., and Saglio, P. (1998). Evidence for the critical role of sucrose synthase for anoxic tolerance of maize roots using a double mutant. Plant Physiol 116, 1323-1331. Rook, F., Corke, F., Card, R., Munz, G., Smith, C., and Bevan, M.W. (1998). Sucrose-specific signaling represses translation of the Arabidopsis ATB2 bZIP transcription factor gene. Plant J 15, 253-263. Romer, U., Schrader, H., Gunther’ N., Nettelstroth, N., Frommer’, W.B., and Lothar Elling, L. (2004). Expression, purification and characterization of recombinant sucrose synthase 1 from Solanum tuberosum L. for carbohydrate engineering. J Biotechnol 107, 135-149. Ruan, Y.L., Llewellyn, D.J., and Furbank, R.T. (2003). Suppression of sucrose synthase gene expression represses cotton fiber cell initiation, elongation, and seed development. Plant Cell 15, 952-964. Sakamoto, M., Satozawa, T., Kishimoto, N., Higo, K., Shimada, H., and Fujimura, T. (1995). Structure and RELP mapping of a rice sucrose-phosphate synthase (SPS) gene that is specifically expressed in the source organ. Plant Sci 112, 207-217. Salnikov, V.V., Grimson, M.J., Seagull, R.W., and Haigler, C.H. (2003). Localization of sucrose synthase and callose in freeze-substituted secondary-wall-stage cotton fibers. Protoplasma 221, 175-184. Sayion, Y., Huang, Y.W., Chen, H.W., Liao, Y.C., and Wang, A.Y. (1999). Expression and characterization of rice sucrose synthase in Escherichia coli. Food Sci Agric Chem 1, 122-128. Scofield, G.N., Hirose, T., Gaudron, J.A., Upadhyaya, N.M., Ohsugi, R., and Furbank R.T. (2002). Antiaense suppression of the sucrose transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis. Funct Plant Biol 29, 815-826. Scholes, J., Bundock, N., Wilde, R., and Rolfe, S. (1996). The impact of reduced vacuolar invertase activity on the photosynthetic and carbonhydrate metabolism of tomato. Planta 200, 265-272. Seneweera, S.P., Basra, A.S., Barlow, E.W., and Conroy, J.P. (1995). Diurnal regulation of leaf blade elongation in rice by CO2. Plant Physiol 108, 1471-1477. Shaw, J.R., Ferl, R.J., Baier, J., Sr Clair, D., Carson, C., McCarty, D.R., and Hannah, L.C. (1994). Structural features of the maize sus1 gene and protein. Plant Physiol 106, 1659-1665. Smeekens, S., Wind, J., Hanson, J. (2010). Sucrose: Metabolite and signaling molecule. Phytochemistry 71, 1610-1614. Sturm, A., and Tang, G.Q. (1999). The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. Trends Plant Sci 4, 401-407. Tang, G-Q., Luacher, M., and Sturm, A. (1999). Antisense repression of vacuolar and cell wall invertase in transgenic carrot alters early plant development and sucrose partitioning. Plant Cell 11, 177-189. Toki, S. (1997). Rapid and efficiency Agrobacterium-mediated transformation in rice. Plant Mol Biol Rep 15, 16-21. Tsai, C.Y., and Wang, A.Y. (2003). Identification of rice manganese-dependent protein kinases that phosphorylate sucrose synthase at multiple serine residues. Bot Bull Acad Sin 44, 141-150. Valdez-Alarcon, J.J., Ferrando, M., Salerno, G., Jimenez-Moraila, B., and Herrera-Estrella, L. (1996). Characterization of a rice sucrose- phosphate synthase encoding gene. Gene 170, 217-222. Van den Ende, W., Bolouri-Moghaddam, M. R., Le Roy, K., Xiang, L., Rolland, F. (2010). Sugar signalling and antioxidant net work connections in plant cells. Febs J 277, 2022-2037. Van Handel, E. (1968). Direct microdetermination fo sucrose. Anal Biochem 22, 280-283. Vinci, J., Nolte, K.D., and Koch, K.E. (1993). Sucrose synthase localization during seed and hair development: Cotton ovules as a model system. Plant Physiol 102, 43-45. Wachter, R., Langhans, M., Aloni, R., Gotz, S., Weilmunster, A., Koops, A., Temguia, L., Mistrik, I., Pavlovkin, J., and Rascher, U. (2003). Vascularization, high-volume solution flow, and localized roles for enzymes of sucrose metabolism during tumorigenesis by Agrobacterium tumefaciens. Plant Physiol 133, 1024-1037. Wang, A.Y., Yu, W.P., Juang, R.H., Huang, J.W., Sung, H.Y., and Su, J.C. (1992). Presence of three rice sucrose synthase genes as revealed by cloning and sequencing of cDNA. Plant Mol Biol 18, 1191-1194. Wang, F., Smith, A.G., and Brenner, M.L. (1994). Temporal and spatial expression pattern of sucrose synthase during tomato fruit development. Plant Physiol 104, 535-540. Wang, A.Y., Kao, M.H., Yang, W.H., Sayion, Y., Liu, L.F., Lee, P.D., and Su, J.C. (1999). Differentially and developmentally regulated expression of three rice sucrose synthase genes. Plant Cell Physiol 40, 800-807. Weschke, W., Panitz, R., Gubatz, S., Wang, Q. Radchuk, R., and Wobus, U. (2003). The role of invertases and hexose transporters in controlling sugar ratios in maternal and filial tissues of barley caryopses during early development. Plant J 33, 395-411. Winter, H., Huber, J.L., and Huber, S.C. (1997). Membrane association of sucrose synthase: changes during the graviresponse and possible control by protein phosphorylation. FEBS Lett 420, 151-155. Winter, H., Huber, J.L., and Huber, S.C. (1998). Identification of sucrose synthase as an actin-binding protein. FEBS Lett 430, 205-208. Wobus, U., and Weber, H. (1999). Sugars as signal molecules in plant seed development. Biol Chem 390, 937-944. Wolosiuk, R.W., and Pontis, H.G. (1971). Evidence of the existence of two forms of sucrose synthetase. FEBS Lett 16, 237-240. Worrell, A.C., Bruneau, J.M., Summerfelt, K., Boersig, M., and Voelker, T.A. (1991). Expression of maize sucrose phosphate synthase in tomato alters leaf carbonhydrate partitioning. Plant Cell 3, 1121-1130. Xu S.M., Brill E., Llewellyn D.J., Furbank R.T., Ruan Y.L. (2012). Overexpression of a potato sucrose synthase gene in cotton accelerates leaf expansion, reduces seed abortion, and enhances fiber production. Mol Plant 5, 430-41. Yu, S.F.,Wang, A.Y., Juang, R.H., Sung, H.Y., and Su, J.C. (1992). Isolation and sequence of rice sucrose synthase cDNA and genomic DNA. Plant Mol Biol 18, 139-142. Zhai, W.X., Chen, C.Y., Zhu, X.F., Chen, X.W., Zhang, D.C., Li, X.B. and Zhu, L.H. (2004). Analysis of T-DNA loci and bacterial blight resistance effects of the trangene Xa21 in transgenic rice. Theor Appl Genet 109, 534-542. Zhang, X.Q., Lund, A.A., Sarath, G., Cerny, R.L., Roberts, D.M., and Chollet, R. (1999). Soybean nodule sucrose synthase (nodulin-100): further analysis of its phosphorylation using recombinat and authentic root-nodule enzymes. Arch Biochem Biophys 371, 70-82. Zrenner, R., Salanoubat, M., Willmitzer, L., and Sonnewald, U. (1995). Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.) Plant J 7, 97-107. 莊榮輝 (1985) 水稻蔗糖合成酶之研究-其純化、生物化學及免疫性質之比較。博士論文,國立台灣大學農業化學研究所。 岩素芬 (1992) 水稻蔗糖合成異構酶之純化與鑑定。博士論文,國立台灣大學農業化學研究所。 陳任道 (1995) 水稻蔗糖合成酶異構基因之研究。博士論文,國立台灣大學農業化學研究所。 郭益全、范明仁 (1999) 稻種原演化與環境適應性。環境與稻作生產。臺灣省農業試驗所。33-51。 廖憶純 (2002) 水稻懸浮培養細胞中蔗糖合成酶基因表現受糖調控之研究。博士論文,國立台灣大學農業化學研究所。 蔡承佳 (2003) 蛋白質磷酸化對水稻蔗糖合成酶酵素功能及基因表現的影響。博士論文,國立台灣大學農業化學研究所。 黃德宜 (2003) 水稻蔗糖合成酶 RSuS3 基因表現與酵素功能之探討。博士論文,國立台灣大學農業化學研究所。 張德清 (2006) 具不同蔗糖合成酶 RSuS3 表現量之轉殖水稻建立及性狀分析。碩士論文,國立台灣大學微生物化學研究所。 黃卓萱 (2007) 水稻蔗糖合成酶 RSuS3 突變株之分析與結構性質探討。碩士論文,國立台灣大學微生物化學研究所。 邱文彬 (2008) 綠竹蔗糖合成酶異構酶之基因表現與生理功能探討。博士論文,國立台灣大學微生物與生化學研究所。 張睿哲 (2011) 水稻蔗糖合成酶 RSuS1 之研究:受糖調控之基因表現與細胞內定位。博士論文,國立台灣大學生化科技學系。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17691 | - |
dc.description.abstract | 在植物中,蔗糖合成酶可催化蔗糖與 UDP 形成果糖與 UDP-glucose 的可逆反應。在水稻中,有六種不同的蔗糖合成酶基因 (RSus1-RSus6),而其中 RSus3 主要表現於水稻種子內。為了瞭解 RSus3 之生理功能,本實驗室先前已建構在水稻中恆常表現 RSus3 及在發育種子中過量表現 RSus3 之水稻轉殖株,然而在轉殖株中,RSus3 轉基因具有一個胺基酸突變 (F680S),會導致轉基因表現之 RSuS3 活性降低。本論文藉由 RSuS3(F680S) 轉殖水稻研究異位表現 (ectopic expression) 失活之RSuS3(F680S) 突變株對水稻生長的影響。另外也建構 RSus3 表現受抑制的 RNAi 轉殖株來探討抑制種子內 RSus3表現對水稻的影響。
針對不同轉殖水稻,其子代已篩選出具單拷貝轉基因之同型合子,並找出其轉基因插入染色體位置。由野生型水稻 (台農 67 號,TNG67) 種子發育時期的蔗糖合成酶異構酶表現分析,RSus3 主要在種子發育初期表現。以 real-time reverse transcription-polymerase chain reaction (qPCR) 與免疫呈色分析 RNAi 轉殖水稻中 RSus3 表現量的差異,TNG67 水稻植株之內生性 RSus3 主要表現在授粉後第 7 天的種子中,而在 RNAi 轉殖株中其內生性 RSus3表現則是受到抑制。在授粉後第 7 與 14 天的種子中,RNAi 轉殖株之蔗糖合成酶活性分別比野生型下降 53-63% 和 43-52%。RNAi 轉殖株之授粉後 7 天種子內果糖含量比 TNG67增加,但蔗糖含量與成熟種子內澱粉含量則較野生型下降。RNAi 轉殖水稻的生長外觀與 TNG67 相比並無差異,只有充實率比野生型低 5-11% 以及種子發芽率出現 24 小時之延遲。以 actin 啟動子控制RSus3(F680S) 轉基因表現之轉殖水稻,其葉片分別以qPCR 與免疫呈色分析顯示具有轉基因表現之 mRNA 與蛋白質。轉殖株之葉片內蔗糖合成酶活性與植株高度均比野生型下降。除此之外,轉殖株葉片外觀也出現改變。轉殖水稻之農藝性狀也與 TNG67 不同。由這些結果推測 RSuS3 對於種子發育初期內蔗糖/六碳糖濃度的動態平衡與澱粉合成具有重要的影響,而且 RSuS 活性對於水稻正常生長也扮演重要的角色。 關鍵詞:蔗糖合成酶、RSus3、轉殖株、基因表現、酵素活性、糖含量、農藝性狀 | zh_TW |
dc.description.abstract | In plants, sucrose synthase (SuS) catalyzes the reversible conversion of sucrose and UDP into UDP-glucose and fructose. In rice, six genes (RSus1-6) encoding SuS have been identified and RSus3 is predominantly expressed in the developing seeds. To elucidate the physiological roles of RSus3, our laboratory had previously constructed different rice transgenic plants, which constitutively expressed RSuS3 in various tissues and overexpressed RSuS3 in developing seeds, respectively. However, in these transgenic plants, the RSus3 transgene had an amino acid mutation (F680S), which resulted in a decrease in enzymatic activity of SuS. This study is aimed at investigating the effect of ectopic expression of an inactive RSuS3(F680S) mutant on the growth of rice using RSus3(F680S) transgenic plants. In addition, the effect of suppression of the RSus3 expression in rice seeds was also investigated by using the RSus3 RNAi transgenic plants.
For different transgenic rice plants, the homozygous progeny lines with single-copy transgene inserted were selected, and the location of the transgene insertion in chromosome was identified. According to the expression analysis of different RSus genes in the wild-type rice (TNG67), RSus3 is mainly expressed at the early stage of seed development. Real-time reverse transcription-polymerase chain reaction (qPCR) and western analysis were conducted to determine the expression levels of RSus3 in TNG67and RSus3-RNAi transgenic plants, respectively. In TNG67 rice plants, the transcripts of RSus3 were mainly expressed in the developing seeds at 7 day after pollination (DAP), while they were reduced in the RNAi transgenic lines. SuS activities were reduced by 53-63% and 43-52% in the 7-DAP and 14-DAP seeds of RNAi plants as compared to the wild-type plant. The level of fructose in seeds at 7- DAP of RNAi mutants was higher than that of TNG67, but the levels of sucrose in 7-DAP seeds and starch in mature seeds were less than wild type. The phenotype of RNAi transgenic plants showed no difference from that of the wild type, except that the seed fertility was 5-11% lower and the germination had a 24-hour delay. In the leaves of transgenic rice plants carrying the RSus3(F680S) transgene under the control of actin promoter, the mRNA and proteins expressed from the transegnes was detected by qPCR and western analysis, respectively. The SuS activity in the leaves and the plant height in the mutant plants were lower than those of the wild type. Besides, the leaf morphology of the transgenic plants was altered. The agricultural properties of transgenic rice plant also differenent from those of TNG67 plants. These results suggest that RSuS3 is important for homeostasis of sucrose/hexose ratio and starch synthesis in seed development, and SuS activity is important for the normal development of rice plants. Keywords: sucrose synthase, RSus3, transgenic plants, gene expression, enzyme activity, sugar content, agricultural properties | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T00:31:16Z (GMT). No. of bitstreams: 1 ntu-102-D93b47203-1.pdf: 5980688 bytes, checksum: aeb71e1953125d712f4da270cef96643 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 目錄...................................................................................................................................I
縮寫表..........................................................................................................................VII中文摘要........................................................................................................................IX 英文摘要..........................................................................................................................X 第一章 緒論.....................................................................................................................1 第一節 蔗糖在植物中的生理角色...............................................................................1 1.1 植物主要醣類運輸形式 1 1.2 能量儲存與訊息傳導 1 1.3 蔗糖合成與代謝相關酵素 2 第二節 蔗糖合成酶.........................................................................................................3 2.1 SuS催化之反應3 2.2 SuS異構酶與其基因 4 2.3 SuS 異構酶的組織特異性 4 2.4 SuS 的磷酸化 5 2.5 SuS 的生理功能 6 2.5.1 參與澱粉之生合成 6 2.5.2 參與細胞壁多醣類之合成 7 2.5.3 參與蔗糖運輸 8 2.5.4 參與逆境反應 8 2.5.5 SuS 異構酶的轉殖植物研究9 第三節 水稻蔗糖合成酶..............................................................................................10 3.1 RSus 基因及異構酶 10 3.1.1 RSuS1 11 3.1.2 RSuS2 11 3.1.3 RSuS3 12 3.1.4 RSuS4-RSuS6異構酶基因 12 3.2 本實驗室其他蔗糖合成酶的研究 12 第四節 水稻...................................................................................................................13 4.1 水稻簡介13 4.2 水稻生長 13 第五節 研究目的與方向..............................................................................................14 5.1研究緣起與目的 14 5.2 研究方向與主題16 5.2.1 RSus4、RSus5 與 RSus6 之 cDNAs 選殖 16 5.2.2 水稻蔗糖合成酶異構酶 RSuS1-RSuS6 專一性抗體製備 16 5.2.3 RSuS3 基因轉殖水稻分析 16 5.2.4 RSus3 抑制表現轉殖水稻與 TNG67 之比較 16 5.2.5 表現 RSus3(F680S) 轉殖水稻與 TNG67 之比較 17 5.2.6 種子發芽期間 RSus 異構基因之表現 17 第二章 材料與方法.......................................................................................................19 第一節 實驗材料與樣品...............................................................................................19 1.1 植物材料 19 1.2 菌種 19 1.3 質體 19 1.4 藥品 19 第二節 實驗儀器設備...................................................................................................19 2.1 水平核酸電泳 19 2.2 蛋白質電泳、轉印設備 20 2.3 離心機 20 2.4 其他 20 第三節 實驗流程與方法...............................................................................................20 3.1 水稻之轉殖 20 3.1.1水稻癒合組織誘導 20 3.1.2 農桿菌勝任細胞製備與電轉形 21 3.1.3 農桿菌對水稻癒合組織之感染 21 3.1.3.1農桿菌之大量培養 21 3.1.3.2 農桿菌水稻癒合組織共培養 22 3.1.3.3 清洗癒合組織 22 3.1.3.4 第一次篩選 22 3.1.3.5 第二次篩選 22 3.1.3.6 分化與移植 22 3.1.4 水稻種子發芽 23 3.1.5農藝性狀分析與種子保存 23 3.2 水稻染色體 DNA 之 抽取與分析方法 23 3.2.1 水稻染色體 DNA 之抽取 23 3.2.2水稻染色體 DNA 之限制酶作用 24 3.2.3水稻染色體 DNA 瓊脂糖膠體電泳分析 24 3.2.4 轉印 24 3.2.5 放射線標定探針之製備 25 3.2.6雜合反應 25 3.2.7聚合酶連鎖反應 25 3.2.8熱不對稱交錯聚合酶連鎖反應 26 3.2.8.1 Primary TAIL-PCR 27 3.2.8.2 Secondary TAIL-PCR 28 3.7.8.3 Third TAIL-PCR 28 3.3 水稻 RNA 之 抽取與分析方法 29 3.3.1 水稻 total RNA 之抽取 29 3.3.2 殘留 DNA 之去除 29 3.3.3 RNA 之定量法 30 3.3.4 反轉錄反應 30 3.3.5 甲醛瓊脂糖膠體電泳 30 3.3.6 即時反轉錄聚合酶反應 30 3.4 水稻蛋白質抽取、定量與酵素活性測定 31 3.4.1 水稻種子蛋白質抽取 31 3.4.1.1 蛋白質粗抽液定量 31 3.4.2 蔗糖合成酶之活性分析 32 3.4.3蔗糖磷酸合成酶之活性分析 32 3.4.4蔗糖轉化酶之活性分析 33 3.4.5 Western 分析 33 3.4.5.1 SDS-PAGE 電泳 33 3.4.5.2蛋白質轉印34 3.4.5.3免疫呈色法34 3.5 相關糖類與澱粉分析 35 3.5.1葡萄糖與 6-磷酸葡萄糖含量 35 3.5.2果糖含量35 3.5.3 蔗糖含量 36 3.5.4 UDPG 含量 36 3.5.5 澱粉含量分析 36 3.6. 抗體製備 37 3.6.1 Monospecific抗原 37 3.6.2 Monospecific抗體製備 38 3.6.3 抗體效價與專一性測試 38 3.7 免疫共沈澱法 38 3.7.1 Protein A-Sepharose 膠體溶液之配製 38 3.7.2免疫共沈澱反應 39 第三章 結果與討論.......................................................................................................40 第一節 RSus4、RSus5 及 RSus6 之 cDNAs 選殖..................................................40 1.1以 RT-PCR 進行 cDNA 全長選殖 40 1.2 RSus1-RSus6 cDNA 結構與序列分析 40 第二節 RSsS1-RSuS6 專一性抗體製備....................................................................41 2.1 RSuS1-RSuS3 專一性抗體製備 41 2.2 RSuS4-RSuS6 專一性抗體製備 41 第三節 轉殖水稻的建立.................................................................................42 3.1 轉殖效率 42 3.2 轉殖株命名 43 3.3 抑制種子內 RSus3 基因表現轉殖水稻 R 之分子鑑定 43 3.3.1 T0 轉殖株 hpt II 基因之檢定 43 3.3.2 T1 轉殖株種子充實期之 RT-PCR 分析 43 3.3.3 T1 轉殖株 Southern 分析 44 第四節 轉殖水稻單拷貝數同型合子 (Homozygotes) 分析...............................44 4.1 轉殖株 T-DNA 插入位置確認 44 4.2 轉殖株單拷貝數同型合子 (homozygotes) 挑選 45 第五節 抑制 RSus3 表現之轉殖水稻 R 生理性狀分析..........................................45 5.1 種子發育期內生性 RSus3 表現量分析 45 5.2 抑制內生性 RSus3 表現對其他 RSus 基因表現之影響 46 5.3 種子發育期間 RSuS 活性與相關醣類之分析 47 5.4 種子發育期內澱粉、種子乾重與含水量之變化 49 5.5 農藝性狀分析 49 5.6 抑制內生性 RSus3 表現對種子發芽之影響 50 第六節 恆常與過量表現 RSus3(F680S) 之轉殖水稻 A 與 G 之生理性狀分析.50 6.1 恆常表現 RSus3(F680S) 對水稻生長之影響 50 6.2 恆常表現 RSus3(F680S) 對水稻幼苗中 RSus 基因表現之分析 51 6.3 恆常表現 RSus3(F680S) 對水稻葉片中 RSuS 活性與外觀之影響 52 6.4 恆常與過量表現 RSus3(F680S) 轉殖水稻之農藝性狀分析 52 6.5 恆常與過量表現 RSus3(F680S) 表現對種子發芽之影響 53 第七節 TNG67 種子發芽時期 RSus 基因表現與相關分析..................................53 7.1 種子發芽時期 RSus 異構酶、Sps 與 Ugp 基因表現 53 7.2 種子發芽時期 RSuS 與 IT 活性的比較 54 7.3種子發芽時期與 RSuS 相關醣類與澱粉含量分析54 第四章 未來展望...........................................................................................................56 4.1 結論 56 4.2 未來展望 56 參考文獻.........................................................................................................................58 圖與表............................................................................................................................66 附錄...............................................................................................................................121 | |
dc.language.iso | zh-TW | |
dc.title | 具不同蔗糖合成酶 RSuS3 表現量之轉殖水稻性狀分析 | zh_TW |
dc.title | Characterization of Transgenic Rice Plants Expressing Various Abundance of Rice Sucrose Synthase RSuS3 | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 博士 | |
dc.contributor.coadvisor | 宋賢一 | |
dc.contributor.oralexamcommittee | 陳慶三,張珍田,張世宗,廖憶純 | |
dc.subject.keyword | 蔗糖合成酶,RSus3,轉殖株,基因表現,酵素活性,糖含量,農藝性狀, | zh_TW |
dc.subject.keyword | sucrose synthase,RSus3,transgenic plants,gene expression,enzyme activity,sugar content,agricultural properties, | en |
dc.relation.page | 124 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2013-07-02 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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