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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17566完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄧麗珍(Lee-Jene Teng) | |
| dc.contributor.author | Po-Yu Li | en |
| dc.contributor.author | 李柏煜 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:21:43Z | - |
| dc.date.copyright | 2013-09-24 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-07-22 | |
| dc.identifier.citation | 1. Lowy, F.D., et al. (1998). “Staphylococcus aureus infections.” N Engl J Med 399(8): 520-32.
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(1999). “Distribution of insertion sequence-like element IS1272 and its position relative to methicillin resistance genes in clinically important staphylococci.” Antimicrob. Agents Chemother. 43: 2780-2782 46. Morikawa, K., et al. (2001). 'Overexpression of sigma factor, sigma(B), urges Staphylococcus aureus to thicken the cell wall and to resist beta-lactams.' Biochemical and Biophysical Research Communications 288(2): 385-389. 47. Steven Y. C. Tong., et al. (2013) 'Virulence of endemic nonpigmented northern Australian Staphylococcus aureus clone (clonal complex 75, S. argenteus) is not augmented by staphyloxanthin.” Journal of Infectious Disease. 48. Chih-Jung Chen, et al. (2009) “Change in the molecular epidemiology of Methicillin-resistant Staphylococcus aureus bloodstream infection in Taiwan.” Diagnostic Microbiology and Infectious Disease. 65(2): 199-201. 49. Wang, J. L., et al. (2010). 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J., et al. (2013) “Agr function is upregulated by photodynamic therapy (PDT) for Staphylococcus aureus and is related to resistance against PDT.” Microbiology and Immunology. 60. Ho, C. M., et al. (2010). 'Prevalence and accessory gene regulator (agr) analysis of vancomycin-intermediate Staphylococcus aureus among methicillin-resistant isolates in Taiwan—SMART program, 2003.' European Journal of Clinical Microbiology & Infectious Diseases 29(4): 383-389. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17566 | - |
| dc.description.abstract | 從抗生素發明至今，人類由於抗生素的濫用導致抗藥性細菌的爆炸性成長，，特別是MRSA(Methicillin-resistant Staphylococcus aureus)。因此，科學家便回過頭來尋找一些古老的細菌治療方法，企圖扭轉此一頹勢。其中一項令人注目的古老療法就是所謂的光動力治療(Photodynamic inactivation)。光動力的原理是利用特定波長的光激發光感物質(Photosensitizer)，並藉著電子及能量轉移來產生自由基(Free radical)和單態氧(Singlet oxygen)來破壞細菌的細胞壁、細胞膜、胞器以及核酸等物質，進而造成細菌死亡。
有研究指出，MRSA針對光動力殺菌的感受性具有strain-dependent的特性。西元2010年Grinholc et al.評估了細菌uptake光感物質的能力以及其NorA efflux pump，發現這不是主要因素。同年，Nakonieczna et al.則是針對Superoxide dismutase進行研究，也認為這個酵素不是直接影響細菌對光動力感受性的直接因素。由於MRSA具多種基因型，不同基因型有不同特性，本研究擬探討TBO光動力對不同SCCmec之MRSA是否具不同殺菌力及可能的原因。利用Bactericidal assay分析了79株MRSA臨床分離株，發現SCCmec IV對於光動力殺菌較具有抵抗性，而SCCmec III對光動力的感受性則最佳。 由於S. aureus最明顯的特徵—金黃色葡萄球菌色素(Staphyloxanthin)也是具有幫助細菌本身抵抗氧化壓力的能力，而在本實驗觀察到不同的菌株對光動力效應的確有不同的感受性，並且感受性較佳的菌株其菌落較為蒼白；感受性差的菌株菌落較黃。經過色素萃取並初步定量之後發現，金黃色葡萄球菌的色素對光動力效應具有保護的效果。因此本實驗中可以得知：金黃色葡萄球菌對光動力反應的確有strain-dependent的現象，並且SCCmec IV較具抗性。其次，金黃色葡萄球菌色素對光動力有保護的效果，而不同SCCmec type的菌株色素表現量也可能有所差異。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:21:43Z (GMT). No. of bitstreams: 1 ntu-102-R00424003-1.pdf: 5477669 bytes, checksum: 4bbb25fb81afa6dc9afbfd6346dd4ab0 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 致謝………………………………………………………………………i
中文摘要…………………………………………………………………ii 英文摘要…...……………………………………………………………iv 目錄…………………...…………………………………………………vi 圖目錄…………………………………………………………………..vii 表目錄………………………………………………………………….viii 第一章、緒論……………………………………………………………1 第一節、MRSA…………………………………………………….1 第二節、光動力治療………………………………………………3 第三節、金黃色葡萄球菌色素……………………………………5 第二章、研究動機與實驗設計…………………………………………7 第一節、研究動機…………………………………………………7 第二節、實驗設計…………………………………………………7 第三章、材料與方法……………………………………………………8 第四章、實驗結果……………………………………………………..21 第五章、實驗討論……………………………………………………..29 第六章、實驗結果附圖………………………………………………..38 第七章、實驗結果附表………………………………………………..47 第八章、附錄…………………………………………………………..51 第九章、參考文獻……………………………………………………..58 | |
| dc.language.iso | zh-TW | |
| dc.title | TBO對Methicillin抗藥金黃色葡萄球菌之光動力殺菌效應 | zh_TW |
| dc.title | TBO Photodynamic Inactivation on Methicillin-resistant Staphylococcus aureus Isolates | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖淑貞(Shwu-Jen Liaw),邱浩傑,蔡瑞章,曾嵩斌 | |
| dc.subject.keyword | 光動力,金黃色葡萄球菌, | zh_TW |
| dc.subject.keyword | Photodynamic,MRSA, | en |
| dc.relation.page | 62 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-07-22 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
| 顯示於系所單位： | 醫學檢驗暨生物技術學系 | |
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