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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳進庭 | |
| dc.contributor.author | Mei-Yan Chen | en |
| dc.contributor.author | 陳玫延 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:14:48Z | - |
| dc.date.copyright | 2013-08-07 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-07-31 | |
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Sinsheimer, Inhibition of protein synthesis in E. coli protoplasts by actinomycin-D. J Mol Biol, 1963. 6: p. 247-9. 46. MacAlister, T.J., J.W. Costerton, and K.J. Cheng, Effect of the removal of outer cell wall layers on the actinomycin susceptibility of a gram-negative bacterium. Antimicrob Agents Chemother, 1972. 1(5): p. 447-9. 47. Coratza, G. and A.M. Molina, Enhanced sensitivity to the photodynamic effect of 3,4-benzpyrene in ethylenediaminetetraacetate-treated Escherichia coli. J Bacteriol, 1978. 133(1): p. 411-2. 48. Ferro, S., et al., Efficient photoinactivation of methicillin-resistant Staphylococcus aureus by a novel porphyrin incorporated into a poly-cationic liposome. International Journal of Biochemistry & Cell Biology, 2007. 39(5): p. 1026-1034. 49. Merchat, M., et al., Meso-substituted cationic porphyrins as efficient photosensitizers of gram-positive and gram-negative bacteria. J Photochem Photobiol B, 1996. 32(3): p. 153-7. 50. Minnock, A., et al., Photoinactivation of bacteria. Use of a cationic water-soluble zinc phthalocyanine to photoinactivate both gram-negative and gram-positive bacteria. J Photochem Photobiol B, 1996. 32(3): p. 159-64. 51. Suci, P.A., et al., Investigation of Ciprofloxacin Penetration into Pseudomonas-Aeruginosa Biofilms. Antimicrob Agents Chemother, 1994. 38(9): p. 2125-2133. 52. Duguid, I.G., et al., Effect of Biofilm Culture Upon the Susceptibility of Staphylococcus-Epidermidis to Tobramycin. Journal of Antimicrobial Chemotherapy, 1992. 30(6): p. 803-810. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17467 | - |
| dc.description.abstract | 日常環境周遭甚至人體身上存在的許多伺機性感染源易感染免疫力本身就低的住院病人，且對其威脅性高。這些病原菌的治療傳統上是使用抗生素，但近年來抗生素濫用導致的抗藥性問題日益嚴重。光動力治療（photodynamic therapy, PDT）應用於感染性疾病治療又稱為光動力殺菌（photodynamic inactivation, PDI），使用於PDI之光感物質中有許多屬於疏水性物質，其於水相溶液中的聚集現象會降低PDI效應。本研究以疏水性光感物質chlorin e6（Ce6）為對象，期望透過微胞（micelle）包埋的策略改善此一問題並增進對三株院內感染常見菌株之PDI效應。菌株分別為革蘭氏陽性菌之金黃色葡萄球菌（Staphylococcus aureus）、革蘭氏陰性菌之綠膿桿菌（Pseudomonas aeruginosa）、真菌之白色念珠菌（Candida albicans）。吸收和螢光光譜、粒徑、Ce6回收率等特性分析結果顯示，三種微胞材料Pluronic P123、Lutrol F127、Synperonic L121中P123最適於Ce6的包埋，此劑型確實能增進對S. aureus之光動力殺菌效果，但對P. aeruginosa和C. albicans仍效果不佳，micelle form Ce6之Reactive oxygen species（ROS）產率較高，但與S. aureus結合量較低，可知微胞的包埋能透過分散Ce6分子增進PDI效應。為增進另外二株菌之PDI效果，改良微胞配方為帶正電荷P123-CTAB micelle Ce6，此一劑型增進了對C. albicans而非P. aeruginosa的PDI效果，由C. albicans與Ce6分子結合量分析結果推測雖然攝入較多free form Ce6但其於菌體內仍為聚集狀態，導至PDI效應不佳。最後我們將帶正電荷micelle Ce6應用於S. aureus和C. albicans生物膜之PDI測試，推測由於extracellular matrix之保護使PDI效果仍不佳。 | zh_TW |
| dc.description.abstract | Opportunistic pathogens will cause disease when the function of immune system is reduced. Due to the extensive use of antibiotics, drug-resistant microbia spread fast. Photodynamic inactivation (PDI) is a potential treatment for infectious disease. Most of the photosensitizers (PSs) are hydrophobic and easy to aggregate, which will reduce the PDI efficacy. To increase the PDI efficacy, we encapsulated chlorin e6 (Ce6) into micelle and explored the PDI efficacy of micellar Ce6 against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Pseudomonas aeruginosa, and fungus Candida albicans, as well as the biofilm. The UV-Vis and fluorescence spectrophotometric spectra show that the three materials PF127, L121 and P123 can successfully encapsulate and disperse Ce6. Micellar Ce6 can improve PDI efficacy to S. aureus, but not to P. aeruginosa and C. albicans. Though P123 micelle Ce6 can produce more reactive oxygen species, their binding to S. aureus was less compared to free-form Ce6.
To improve the PDI efficacy against P. aeruginosa and C. albicans, cationic P123-CTAB micelle Ce6 was developed. No dark toxicity was found in P123-CTAB micelle Ce6 but cause significant killing against C. albicans after light irradiation. Compared to free-form Ce6, P123-CTAB micelle Ce6 induce higher PDI effect, though C. albicans uptake higher content of free-form Ce6. The lower PDI efficacy might relate to the aggregation of free Ce6. Finally, we found that P123-CTAB micelle Ce6 can not improve PDI efficacy against the biofilms of S. aureus and C. albicans, which might relate to the protection of extracellular polymeric substances (EPS). | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:14:48Z (GMT). No. of bitstreams: 1 ntu-102-R00b22031-1.pdf: 1319485 bytes, checksum: dfe4743904e736a866df403db837eae0 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 摘要 I
Abstract II 圖目錄 V 表目錄 VI 第一章、 緒論 1 1. 微生物與人類 1 1.1人體正常菌叢（Normal flora） 1 1.2伺機性感染（Opportunistic infection） 2 1.3院內感染 2 2.光動力治療(Photodynamic therapy, PDT) 3 2.1發展起源 3 2.2作用機制 4 2.3本研究使用之光感物質chlorin e6（Ce6） 6 2.4光動力殺菌(Photodynamic inactivation, PDI) 6 3.高分子微胞(Polymeric micelle) 7 4.本研究使用菌種介紹 9 4.1金黃色葡萄球菌(Staphylococcus aureus) 9 4.2綠膿桿菌(Pseudomonas aeruginosa) 9 4.3白色念珠菌(Candida albicans) 10 5.實驗動機與目的 11 第二章、材料與方法 12 1.藥品 12 2.儀器 13 3.菌種來源與保存 14 3.1菌種來源 14 3.2菌種保存與活化 14 4.實驗方法 15 4.1微胞製備 15 4.2 Ce6定量及回收率分析 15 4.3 Micelle光譜性質分析 16 4.4 Micelle粒徑分析 16 4.5懸浮菌體培養 16 4.6懸浮菌體之光動力殺菌 16 4.7菌體與光感物質結合量分析 17 4.8生物膜之光動力殺菌 17 第三章、結果 18 1.光感物質特性分析 18 1.1 chlorin e6於不同溶劑中之光譜性質 18 2. 微胞包埋Ce6之性質分析 19 2.1光譜性質分析 19 2.2回收率與粒徑分析 19 3. Micellar Ce6應用於懸浮菌體之光動力殺菌 20 3.1金黃色葡萄球菌 20 3.2綠膿桿菌 20 3.3白色念珠菌 20 4.帶正電微胞包埋Ce6之性質分析 21 4.1光譜性質分析 21 4.2回收率、粒徑、帶電量分析 22 5.帶正電Micellar Ce6應用於光動力殺菌 22 5.1懸浮菌體 22 5.1.1綠膿桿菌 22 5.1.2白色念珠菌 23 5.1.3金黃色葡萄球菌 24 6.Micelle form Ce6增進光動力效應的可能作用機制 24 6.1Micellar Ce6之ROS產率分析 24 6.2菌體與光感物質Ce6結合量分析 25 7.Micelle form Ce6應用於生物膜之光動力殺菌 26 7.1金黃色葡萄球菌 26 7.2白色念珠菌 26 第四章、討論 27 1. 微胞包埋Ce6之基本性質 27 2. 中性微胞與帶正電微胞 30 3. 微胞包埋Ce6應用於光動力殺菌之成效 31 圖表 35 附錄 60 | |
| dc.language.iso | zh-TW | |
| dc.title | 以微胞包埋chlorin e6之光動力殺菌研究 | zh_TW |
| dc.title | Photodynamic inactivation against bacteria and fungi by micellar chlorin e6 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃慶燦,蔡翠敏,許瑞祥 | |
| dc.subject.keyword | 光動力殺菌,chlorin e6,微胞,Pluronic P123,金黃色葡萄球菌,綠膿桿菌,白色念珠菌, | zh_TW |
| dc.subject.keyword | Photodynamic inactivation,chlorin e6,micelle,Pluronic P123,Staphylococcus aureus,Pseudomonas aeruginosa,Candida albicans, | en |
| dc.relation.page | 65 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-07-31 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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