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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭石通(Shih-Tong Jeng) | |
| dc.contributor.author | Kai-Hung Cheng | en |
| dc.contributor.author | 鄭凱鴻 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:03:30Z | - |
| dc.date.copyright | 2013-08-29 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-14 | |
| dc.identifier.citation | Akman, Z. (2009). Comparison of high temperature tolerance in maize, rice and sorghum seeds by plant growth regulators. Journal of Animal and Veterinary Advances 8, 358-361.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17258 | - |
| dc.description.abstract | miRNA是小片段的non-coding RNA，這些小片段的RNA在調控植物的生長和發育上扮演著相當重要的角色。miR169在不同物種之間是一段高度保守性的序列。在阿拉伯芥當中已知miR169家族一共有14個不同的成員。前人的研究中也指出miR169會參與在不同的非生物性逆境之調控當中。除了乾旱和缺氮逆境外，miR169也會參與在逆境當中。miR169的表現會受熱誘導而上升，目標基因轉錄因子Nuclear factor Y（NF-Y）家族中的NF-YA則是會下降。但是，miR169和熱相關的調控機制之研究仍然有限。因此，本實驗的目的是想了解miR169和NF-YA在熱逆境下，如何進行調控進而使植物耐熱。利用35S啟動子大量表現miR169的前驅序列（OE Line），或大量表現一股與mature mir169互補的序列（Target mimicry line） 抑制mir169的活性，進行NF-YA的調控，藉以了解miR169與NF-YA在熱的情況下所扮演的角色。發芽率的檢測於熱處理後，OE Line有較佳的發芽情形。先天性耐熱能力的測試中，Target mimicry line有較高的存活率，相反的，在後天性的耐熱能力測試則是OE Line有較高的存活率。也因此，下胚軸長度檢測OE Line中有些微較長的情形。根長則是部分Target mimicry line有較短的情況。總結以上的結果，miR169可藉由調控NF-YA進而使植物在不同的熱逆境條件下維持其恆定性，進而使植物更耐熱。 | zh_TW |
| dc.description.abstract | MicroRNAs (miRNAs) are small non-coding RNAs, and they play important roles in plant growth and development. miR169 is a conserved miRNA in many plant species, and it contains 14 members of the miR169 family in Arabidopsis. In addition, miR169 is known to be involved in various stress responses, including drought and nitrogen deficient. Previous studies have shown that the expression of miR169 can be induced by heat treatment, but the research on miR169-regulated mechanism in heat response is still limited. Here, we generated transgenics overexpressing miR169 and transgenics expressing target mimic inhibitor, which could inhibit miR169 activity. After heat treatment, the germination rates of seeds were slightly increased in miR169 overexpression lines compared to those in wild type. Under basal thermotolerance, the mimicry lines were also greener than the wild type. But they showed opposite result under acquired thermotolerance. In addition, the hypocotyls lengths of the miR169 overexpression lines were slightly longer compared to those of the wild type plants. Although the miR169 overexpression lines did not show any difference, but the root lengths of miR169 target mimicry lines were shorter than those of the wild types. Overall, these results showed that miR169 might regulate NF-YA expression homeostasis to be involved in different thermotolerance. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:03:30Z (GMT). No. of bitstreams: 1 ntu-102-R00b42036-1.pdf: 1173694 bytes, checksum: 9d25a146629a0dbe361b612b83f37957 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 i 誌謝 ii 摘要 iii Abstract iv 縮寫與中英對照表 v 目錄 vi 圖目錄 ix 第一章 緒論 1 壹、 研究動機 1 一、 熱逆境 1 二、 熱逆境對植物的影響 1 2.1 植物的生長 1 2.2 植物的生殖 2 2.3 植物內部的生理反應 2 三、 熱逆境下植物的生理調控 2 3.1 過氧化物（ROS） 2 3.2 熱休克因子（HSF） 3 3.3 熱休克蛋白（HSP） 3 3.4 賀爾蒙 4 四、 miRNA 5 4.1 miRNA的生合成 5 4.2 miRNA與逆境 5 4.3 miRNA與熱逆境 6 五、 miR169與NF-YA 6 六、 實驗目的 8 第二章 材料與方法 9 壹、 材料 9 貳、 方法 10 一、 DNA 10 1.1 DNA純化 10 1.2 DNA電泳 10 二、 RNA 10 2.1 RNA純化 10 2.2 RNA跑膠 11 三、 聚合酶鏈鎖反應（PCR） 11 3.1 聚合酶鏈鎖反應（RT-PCR) 11 3.2 即時定量聚合酶鏈鎖反應(QRT-PCR) 11 3.3 cDNA合成 11 3.4 Mature miRNA cDNA合成 12 四、 質體構築 12 4.1 限制酶切割 12 4.2 DNA切膠純化 12 4.3 DNA黏合 12 4.4 轉型作用 13 4.5 小量質體抽取 13 4.6 DNA定序 13 五、 農桿菌轉染及阿拉伯芥轉殖 13 5.1 農桿菌勝任細胞製備 13 5.2 農桿菌轉型 14 5.3 阿拉伯芥轉殖 14 六、 阿拉伯芥外表型態與生理分析 14 6.1 無菌播種與熱逆境下發芽率 14 6.2 先天性耐熱能力測試（Basal thermotolerance） 14 6.3 後天性的耐熱能力測試（Acquired thermoltolerance） 15 6.4 熱逆境下胚軸長度檢測 15 6.5 熱逆境根長檢測 15 第三章 結果 16 一、 miR169b於熱的情況下之表現 16 二、 miR169b目標基因於熱的情況下之表現 16 三、 miR169b OE lines之基因檢測 16 四、miR169 target mimicry line 的原理 17 五、miR169轉殖株當中目標基因的檢測 17 六、熱處理後之發芽率檢測 17 七、熱處理之先天性耐熱能力檢測（Basal thermotolerance） 18 八、熱處理之後天性的耐熱能力檢測 （Acquired thermoltolerance） 18 九、熱處理之下胚軸長度檢測 18 十、熱處理之根長檢測 19 第四章 討論 20 一、 miR169與NF-YA的調控。 20 二、 miR169轉殖株與基因表現 20 三、 miR169與發芽 22 四、 miR169與先天性耐熱能力檢測 23 五、 miR169與後天性耐熱能力檢測 24 六、 熱處理下之下胚軸長度檢測 25 七、 熱處理下之根長度檢測 25 圖 27 參考文獻 37 附錄 44 圖目錄 圖一、 miR169b於熱的情況下之表現 27 圖二、 miR169b目標基因於熱的情況下之表現 28 圖三、 miR169b OE line前驅物之基因檢測 29 圖四、 miR169 target mimicry line 的原理 30 圖五、 miR169轉殖珠中目標基因的檢測 31 圖六、熱處理後之發芽率檢測 32 圖七、熱處理之先天性耐熱能力檢測（Basal thermotolerance） 33 圖八、熱處理之後天性的耐熱能力檢測（Acquired thermoltolerance） 34 圖九、熱處理之下胚軸長度檢測 35 圖十、熱處理之根長檢測 36 | |
| dc.language.iso | zh-TW | |
| dc.title | 阿拉伯芥miR169於熱逆境下的功能性分析 | zh_TW |
| dc.title | Functional analyses of miR169 in Arabidopsis under heat stress | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陸重安,黃麗芬 | |
| dc.subject.keyword | miR169,NF-YA,熱逆境,耐熱性,先天性耐熱能力,後天性的耐熱, | zh_TW |
| dc.subject.keyword | miR169,NF-YA,,Heat,Thermotolerance,Basal thermotolerance,Acquired thermotolerance., | en |
| dc.relation.page | 46 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-08-14 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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| ntu-102-1.pdf 目前未授權公開取用 | 1.15 MB | Adobe PDF |
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