請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17191
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭石通(Shih-Tong Jeng) | |
dc.contributor.author | Sheng-Chih Fan | en |
dc.contributor.author | 范盛之 | zh_TW |
dc.date.accessioned | 2021-06-08T00:00:17Z | - |
dc.date.copyright | 2013-08-29 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-16 | |
dc.identifier.citation | Abe, H., Shimoda, T., Ohnishi, J., Kugimiya, S., Narusaka, M., Seo, S., Narusaka, Y., Tsuda, S., and Kobayashi, M. (2009). Jasmonate-dependent plant defense restricts thrips performance and preference. BMC Plant Biol 9, 97.
Albrechtova, J.T.P., and Ullmann, J. (1994). Methyl Jasmonate Inhibits Growth and Flowering in Chenopodium-Rubrum. Biol Plant 36, 317-319. Bai, S., Li, M., Yao, T., Wang, H., Zhang, Y., Xiao, L., Wang, J., Zhang, Z., Hu, Y., Liu, W., and He, Y. (2012). Nitric oxide restrain root growth by DNA damage induced cell cycle arrest in Arabidopsis thaliana. Nitric Oxide 26, 54-60. Bricchi, I., Leitner, M., Foti, M., Mithofer, A., Boland, W., and Maffei, M.E. (2010). Robotic mechanical wounding (MecWorm) versus herbivore-induced responses: early signaling and volatile emission in Lima bean (Phaseolus lunatus L.). Planta 232, 719-729. Chen, W.W., Yang, J.L., Qin, C., Jin, C.W., Mo, J.H., Ye, T., and Zheng, S.J. (2010). Nitric oxide acts downstream of auxin to trigger root ferric-chelate reductase activity in response to iron deficiency in Arabidopsis. Plant Physiol 154, 810-819. Delledonne, M., Xia, Y.J., Dixon, R.A., and Lamb, C. (1998). Nitric oxide functions as a signal in plant disease resistance. Nature 394, 585-588. Desikan, R., Griffiths, R., Hancock, J., and Neill, S. (2002). A new role for an old enzyme: nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana. Proc Natl Acad Sci U S A 99, 16314-16318. Durner, J., Wendehenne, D., and Klessig, D.F. (1998). Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc Natl Acad Sci U S A 95, 10328-10333. Ellis, C., Karafyllidis, I., and Turner, J.G. (2002). Constitutive activation of jasmonate signaling in an Arabidopsis mutant correlates with enhanced resistance to Erysiphe cichoracearum, Pseudomonas syringae, and Myzus persicae. Mol Plant Microbe Interact 15, 1025-1030. Farmer, E.E., Almeras, E., and Krishnamurthy, V. (2003). Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr Opin Plant Biol 6, 372-378. Flores-Perez, U., Sauret-Gueto, S., Gas, E., Jarvis, P., and Rodriguez-Concepcion, M. (2008). A mutant impaired in the production of plastome-encoded proteins uncovers a mechanism for the homeostasis of isoprenoid biosynthetic enzymes in Arabidopsis plastids. Plant Cell 20, 1303-1315. Garces, H., Durzan, D., and Pedroso, M.C. (2001). Mechanical stress elicits nitric oxide formation and DNA fragmentation in Arabidopsis thaliana. Ann Bot 87, 567-574. Godber, B.L.J., Doel, J.J., Sapkota, G.P., Blake, D.R., Stevens, C.R., Eisenthal, R., and Harrison, R. (2000). Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase. J Biol Chem 275, 7757-7763. Guo, F.Q., Okamoto, M., and Crawford, N.M. (2003). Identification of a plant nitric oxide synthase gene involved in hormonal signaling. Science 302, 100-103. Halitschke, R., and Baldwin, I.T. (2004). Jasmonates and related compounds in plant-insect interactions. J Plant Growth Regul 23, 238-245. Hass, C., Lohrmann, J., Albrecht, V., Sweere, U., Hummel, F., Yoo, S.D., Hwang, I., Zhu, T., Schafer, E., Kudla, J., and Harter, K. (2004). The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis. EMBO. J. 23, 3290-3302. Huijser, P., and Schmid, M. (2011). The control of developmental phase transitions in plants. Development 138, 4117-4129. Jih, P.J., Chen, Y.C., and Jeng, S.T. (2003). Involvement of hydrogen peroxide and nitric oxide in expression of the ipomoelin gene from sweet potato. Plant Physiol 132, 381-389. Landgraf, R., Schaarschmidt, S., and Hause, B. (2012). Repeated leaf wounding alters the colonization of Medicago truncatula roots by beneficial and pathogenic microorganisms. Plant Cell Environ 35, 1344-1357. Leon, J., Rojo, E., and Sanchez-Serrano, J.J. (2001). Wound signalling in plants. J. Exp. Bot. 52, 1-9. Li, J.H., Liu, Y.Q., Lu, P., Lin, H.F., Bai, Y., Wang, X.C., and Chen, Y.L. (2009). A signaling pathway linking nitric oxide production to heterotrimeric G protein and hydrogen peroxide regulates extracellular calmodulin induction of stomatal closure in Arabidopsis. Plant Physiol 150, 114-124. Liu, W.Z., Kong, D.D., Gu, X.X., Gao, H.B., Wang, J.Z., Xia, M., Gao, Q., Tian, L.L., Xu, Z.H., Bao, F., Hu, Y., Ye, N.S., Pei, Z.M., and He, Y.K. (2013). Cytokinins can act as suppressors of nitric oxide in Arabidopsis. Proc Natl Acad Sci U S A 110, 1548-1553. Lozano-Juste, J., and Leon, J. (2010). Enhanced abscisic acid-mediated responses in nia1nia2noa1-2 triple mutant impaired in NIA/NR- and AtNOA1-dependent nitric oxide biosynthesis in Arabidopsis. Plant Physiol 152, 891-903. Lozano-Juste, J., and Leon, J. (2011). Nitric oxide regulates DELLA content and PIF expression to promote photomorphogenesis in Arabidopsis. Plant Physiol 156, 1410-1423. Moraes, M.C., Birkett, M.A., Gordon-Weeks, R., Smart, L.E., Martin, J.L., Pye, B.J., Bromilow, R., and Pickett, J.A. (2008). cis-Jasmone induces accumulation of defence compounds in wheat, Triticum aestivum. Phytochemistry 69, 9-17. Moreau, M., Lee, G.I., Wang, Y., Crane, B.R., and Klessig, D.F. (2008). AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase. J. Biol. Chem. 283, 32957-32967. Neill, S.J., Desikan, R., and Hancock, J.T. (2003). Nitric oxide signalling in plants. New Phytol 159, 11-35. Oyarce, P., and Gurovich, L. (2011). Evidence for the transmission of information through electric potentials in injured avocado trees. J. Plant Physiol. 168, 103-108. Pauwels, L., Inze, D., and Goossens, A. (2009). Jasmonate-inducible gene: what does it mean? Trends Plant Sci 14, 87-91. Rasmann, S., and Agrawal, A.A. (2009). Plant defense against herbivory: progress in identifying synergism, redundancy, and antagonism between resistance traits. Curr Opin Plant Biol 12, 473-478. Reinhart, B.J., Weinstein, E.G., Rhoades, M.W., Bartel, B., and Bartel, D.P. (2002). MicroRNAs in plants. Genes Dev 16, 1616-1626. Rossel, J.B., Wilson, P.B., Hussain, D., Woo, N.S., Gordon, M.J., Mewett, O.P., Howell, K.A., Whelan, J., Kazan, K., and Pogson, B.J. (2007). Systemic and intracellular responses to photooxidative stress in Arabidopsis. Plant Cell 19, 4091-4110. Sheard, L.B., Tan, X., Mao, H., Withers, J., Ben-Nissan, G., Hinds, T.R., Kobayashi, Y., Hsu, F.F., Sharon, M., Browse, J., He, S.Y., Rizo, J., Howe, G.A., and Zheng, N. (2010). Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature 468, 400-405. Stamler, J.S., Singel, D.J., and Loscalzo, J. (1992). Biochemistry of nitric oxide and its redox-activated forms. Science 258, 1898-1902. Staswick, P. (2009). Plant hormone conjugation: a signal decision. Plant Signal Behav 4, 757-759. Stohr, C., Strube, F., Marx, G., Ullrich, W.R., and Rockel, P. (2001). A plasma membrane-bound enzyme of tobacco roots catalyses the formation of nitric oxide from nitrite. Planta 212, 835-841. Tsutsui, T., Kato, W., Asada, Y., Sako, K., Sato, T., Sonoda, Y., Kidokoro, S., Yamaguchi-Shinozaki, K., Tamaoki, M., Arakawa, K., Ichikawa, T., Nakazawa, M., Seki, M., Shinozaki, K., Matsui, M., Ikeda, A., and Yamaguchi, J. (2009). DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis. J Plant Res 122, 633-643. Vaucheret, H. (2008). Plant ARGONAUTES. Trends Plant Sci 13, 350-358. Vazquez, F., Legrand, S., and Windels, D. (2010). The biosynthetic pathways and biological scopes of plant small RNAs. Trends Plant Sci 15, 337-345. Voinnet, O. (2009). Origin, biogenesis, and activity of plant microRNAs. Cell 136, 669-687. Wang, J.W., Czech, B., and Weigel, D. (2009). miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana. Cell 138, 738-749. Weitzberg, E., and Lundberg, J.O. (1998). Nonenzymatic nitric oxide production in humans. Nitric Oxide 2, 1-7. Wojtaszek, P. (2000). Nitric oxide in plants. To NO or not to NO. Phytochemistry 54, 1-4. Yamaguchi, A., Wu, M.F., Yang, L., Wu, G., Poethig, R.S., and Wagner, D. (2009). The microRNA-regulated SBP-Box transcription factor SPL3 is a direct upstream activator of LEAFY, FRUITFULL, and APETALA1. Dev Cell 17, 268-278. Zhao, M.G., Tian, Q.Y., and Zhang, W.H. (2007). Nitric oxide synthase-dependent nitric oxide production is associated with salt tolerance in Arabidopsis. Plant Physiol 144, 206-217. Zimmermann, M.R., Maischak, H., Mithofer, A., Boland, W., and Felle, H.H. (2009). System potentials, a novel electrical long-distance apoplastic signal in plants, induced by wounding. Plant Physiol 149, 1593-1600. Zottini, M., Costa, A., De Michele, R., Ruzzene, M., Carimi, F., and Lo Schiavo, F. (2007). Salicylic acid activates nitric oxide synthesis in Arabidopsis. J. Exp. Bot. 58, 1397-1405. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17191 | - |
dc.description.abstract | 為了研究一氧化氮 (NO)在阿拉伯芥傷害下所扮演的訊息傳遞角色,使用傷害處理過的野生型 (WT)與nitric oxide synthase 1 (noa1)突變型阿拉伯芥cDNA進行基因晶片分析,並經過gene spring軟體篩選,將noa1與WT基因表現量達到兩倍以上差異的轉錄調控因子挑選出來進行後續的實驗。從不處理傷害的 noa1/WT晶片中挑選出表現量下降,且在處理傷害下的noa1/WT晶片中表現量下降幅度更大的轉錄調控因子。希望能由晶片的資料中找出經由傷害處理後在WT中基因表現量會受到誘導上升,而在noa1中不會被誘導的轉錄調控因子。經由real-time polymerase chain reaction (real-time PCR)的檢測,找到NAC domain containing protein 96 (ANAC096)、DREB and EAR motif protein 4 (RAP2.10)、WRKY DNA-binding protein (WRKY) 55.1與WRKY55.2等基因具有與晶片資料相符合的基因表現量趨勢。為了確定那些轉錄調控因子是否在傷害下經由NO訊息傳導而誘導表現量上升,而使用了NO釋放劑sodium nitroprusside (SNP)去處理WT與noa1植株,結果顯示ANAC096、WRKY55.1、和WRKY55.2在noa1植株處理SNP後,其基因表現和WT單純傷害後相似,則可以證明這些轉錄調控因子的表現量在傷害後會經由NO的訊息調控所誘導。在另一部分的實驗,將篩選出的轉錄調控因子,與Arabidopsis Small RNA Project網路資料庫比對後,挑選出11個與已知miRNA有連結的目標基因。經過real-time PCR檢測後,確認了四個目標基因的表現量可能與NO相關聯,其中Squamosa promoter binding protein-like (SPL) 3, SPL4和SPL5與晶片上的表現量趨勢符合,而miR156已知可以調控SPL基因,SPL基因與miR156與生長時期轉換和開花調控有關。利用real-time PCR與北方墨點法確認precursor form miR156a與mature form miR156的表現量。然而miR156的表現量卻顯示與NO不相關,經過傷害後的植株顯示出開花時間延後。實驗結果發現傷害處理下SPL基因與miR156與NO不相關,但是傷害可能透過其他的機制去影響開花時間。 | zh_TW |
dc.description.abstract | To study nitric oxide signaling upon wounding, Arabidopsis gene chips were hybridized with cDNA probes from wild-type (WT) Arabidopsis and mutant with noa1 (nitric oxide synthase 1) after wounding. Genes, whose expression levels were different from two folds between WT and mutants, were selected, and, especially, those encoding transcription factors were chosen for further study. The putative transcription factors were chosen from the microarray data whose values were reduced in noa1/WT wounding chip compared to those in noa1/WT non-wounding chip. Hence, genes encoding the transcription factors which were up regulated by wounding in WT plant but not in noa1 mutant were screened for further study. The real-time PCRs were used to confirm the gene expression profiles in microarray, showing the expression patterns of transcription factor NAC domain containing protein 96 (ANAC096), DREB and EAR motif protein 4 (RAP2.10), WRKY DNA-binding protein (WRKY)27, WRKY55.1 and WRKY55.2 were the same as those in microarray. To study the transcription factors induced by nitric oxide through wounding, NO donor sodium nitroprusside (SNP) was used to treat WT and noa1 Arabidopsis. SNP treatment can recover the expression levels of genes encoding ANAC096,WRKY55.1, or WRKY55.2 on noa1 mutant plant after wounding, indicating these transcription factors were involved in the nitric oxide signal pathway induced by wounding. In another part of study, the putative miRNAs for NO-related transcription factors were suggested by Arabidopsis Small RNA Project database, indicating 11 target transcription factors have corresponding miRNAs. The real-time PCRs were used to confirm the gene expression in microarray, showing the expression patterns of transcription factor Squamosa promoter binding protein-like (SPL) 3, SPL4 and SPL5 were the same as those in microarray. It has been known that some of the SPL genes can be regulated by miR156. The expression levels of precursor and mature miR156a, analyzed by real-time PCR and Northern, respectively, upon wounding are different. Moreover, the expression pattern of mature form miR156 was not related to nitric oxide. SPL gene and miR156 have been known to play important roles in regulating phase transition and flowering time. Hence, plants wounded repeatedly showed delay flowering. Conclusively, the effect of wounding on SPL genes and miR156 is not mainly dependent on nitric oxide, but wounding affects SPL gene, miR156 and flowering time by a mechanism needed for further study. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T00:00:17Z (GMT). No. of bitstreams: 1 ntu-102-R00b42035-1.pdf: 1540906 bytes, checksum: a22393d3dcf2402461227f6130c42219 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 中文摘要: I
Abstract: III 目錄 V 圖表與附錄 VII 第一章、前言 1 1. 一氧化氮(NO)在植物中扮演角色 1 1.1 NO訊息傳遞 1 1.2 NO生合成 1 1.3 NOS之功能 2 1.4 NO參與許多生長發育及各種逆境下的調控 3 1.5 noa1性狀特性 3 2. 植物中小分子RNA 4 2.1 小分子RNA分類與生合成 4 2.2 小分子RNA調控目標基因方式 4 2.3 miR156與SPL基因 5 3. 傷害對植物的影響 5 4. 研究目的與方向 6 第二章 材料方法 7 1. 植物材料與處理 7 2. 實驗方法 7 2.1 Total RNA萃取 7 2.2 RNeasy plant mini kit RNA萃取 8 2.3 DNase 處理 8 2.4 RNA cleanup protocol 8 2.5 RNA膠體電泳 9 2.6 RT 9 2.7 PCR 9 2.8 Microarray 10 2.9 Real-time PCR 10 2.10 Small RNA分離 11 2.11 Small RNA電泳 11 2.12 北方墨點法-Small RNA轉印 12 2.13 Small RNA探針製備 12 2.14 Small RNA 雜合反應及分析 13 第三章 結果 14 1. 確認noa1 (nos)T-DNA突變株 14 2. 阿拉伯芥微矩陣晶片 (microarray gene chip) 14 3. 目標基因篩選 15 4. 與NO相關的轉錄調控因子表現量驗證 15 5. SNP藥劑處理回復noa1中目標基因的表現量 16 6. 目標基因篩選(miRNA) 16 7. 與miRNA及NO相關的轉錄調控因子表現量驗證 16 8. 分析傷害下precursor form miR156a在植株中的表現量 17 9. 分析傷害下mature form miR156在植株中的表現量 17 10.重複傷害處理對植株型態影響 17 第四章 討論 19 1. NOA在植物中產生NO 19 2. 微陣列晶片設置 19 3. 添加SNP回復noa1中目標基因的表現量 20 4. miR156a與SPL表現量不一致 21 5. miR156、miR156a與SPL的表現量不一致 21 6. 傷害影響開花 21 7. 總結 22 第五章 參考文獻 37 | |
dc.language.iso | zh-TW | |
dc.title | 利用阿拉伯芥noa1突變株在傷害下研究訊息調控因子 | zh_TW |
dc.title | Study of target genes in noa1 Arabidopsis thaliana by wounding | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃皓瑄(Hau-Hsuan Hwang),陸重安(Chung-An Lu),黃麗芬(Li-Fen Huang),陳玉琪(Yu-Chi Chen) | |
dc.subject.keyword | noa1,一氧化氮,傷害,阿拉伯芥,miR156,轉錄調控因子, | zh_TW |
dc.subject.keyword | noa1,nitric oxide,wounding,Arabidopsis,miR156,transcription factor, | en |
dc.relation.page | 42 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2013-08-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 1.5 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。